BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,828,980	A→C	5.8%	Q12H (CAA→CAC)	xanP →	xanthine permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,828,980	0	A	C	5.8%	1418.6 / 7.0	527	Q12H (CAA→CAC)	xanP	xanthine permease
Reads supporting (aligned to +/- strand): ref base A (250/231); new base C (9/37); total (259/268)
Fisher's exact test for biased strand distribution p-value = 2.07e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 547 total aligned reads displayed.

ATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGG  >  NC_000913/3828831‑3829129

atataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                       >  1:1995421/1‑150 (MQ=255)

  ataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAcc                                                                                                                                                     <  1:5012988/150‑1 (MQ=255)

   taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCg                                                                                                                                                    >  1:7724545/1‑150 (MQ=255)

   taAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGATTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCg                                                                                                                                                    >  2:9089677/1‑150 (MQ=255)

    aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCgg                                                                                                                                                   >  1:8067281/1‑150 (MQ=255)

    aaaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCgg                                                                                                                                                   >  1:9555333/1‑150 (MQ=255)

      aaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGtt                                                                                                                                                 >  1:1024155/1‑150 (MQ=255)

       aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTg                                                                                                                                                >  1:4771441/1‑150 (MQ=255)

             cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGa                                                                                                                                          <  2:4765958/150‑1 (MQ=255)

             cATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGa                                                                                                                                          <  2:9386709/150‑1 (MQ=255)

              aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAc                                                                                                                                         >  1:9096685/1‑150 (MQ=255)

                    ctcATTTAAATATTTAGATGAGGTTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCaaaa                                                                                                                                   <  2:6876768/150‑1 (MQ=255)

                     tcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAc                                                                                                                                  >  1:7224955/1‑150 (MQ=255)

                       aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                                                                                                                <  2:3790197/150‑1 (MQ=255)

                       aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                                                                                                                >  2:4966253/1‑150 (MQ=255)

                       aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                                                                                                                >  2:663509/1‑150 (MQ=255)

                       aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACAAAAAACac                                                                                                                                >  1:368423/1‑149 (MQ=255)

                           aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGaa                                                                                                                            >  2:4182906/1‑150 (MQ=255)

                           aaaTATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGaa                                                                                                                            >  2:5890727/1‑150 (MQ=255)

                              tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTg                                                                                                                         >  2:7778036/1‑150 (MQ=255)

                              tatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTg                                                                                                                         >  2:7276236/1‑150 (MQ=255)

                                tttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAt                                                                                                                       >  1:6025366/1‑150 (MQ=255)

                                    gatgatgatGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                                   <  2:2527742/150‑1 (MQ=255)

                                    gATGTGGTTGTGTCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGATACCACGGGATTTTTTTCGATGTCTGTTTCCACCCTCGAGTCATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAc                                                                                                                   <  2:8921055/150‑1 (MQ=255)

                                       gAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAAACGGGTGCGCACACACAAAAAAGCGAACTGATTTACCgg                                                                                                                >  1:2247775/1‑149 (MQ=255)

                                           aTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGAACTGATTTACCGTCTTg                                                                                                            >  2:3556267/1‑150 (MQ=255)

                                            tgtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGa                                                                                                           <  2:5160957/150‑1 (MQ=255)

                                            tgtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAACACCCAACTGATTTACCGTCATGa                                                                                                           >  1:6719628/1‑150 (MQ=255)

                                             gtgtaaaaTGTTTGCTTTTCTCTTTCGGTCATATAACTTGCCCGCTTTGCTACCACTGTATTTTTTTCTTTTTGTGTTACCACCCTCTAGTAATAAAATGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa                                                                                                          <  1:5242172/145‑1 (MQ=255)

                                             gtgGCAAACGTTTGCTTTTATCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCACGAGTCAGAAAATGCGCCACCGGGTTGGCAGACACAAAAAAACGAACTGAATTAAAGTCTTTaa                                                                                                          >  1:826476/1‑150 (MQ=255)

                                             gtgGAAAACCTTTGCATTTCACTTTCGGTCAGCTAAAATCCCCGCTTTGCTACCACGGGATTGATTACGATGACTGTTACCACCCACGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGaa                                                                                                          <  2:9344105/150‑1 (MQ=255)

                                                gCAAACGTTTGCTATTCACTTTAGGTCATCTAAAAAGCCCGCTTTGCTACCACGGGATAGTTTTCGAATTCTGTTACCACCCTCGAGTCAGAAAAAGCGCAACATGAAGCGCAGACTCAAAACAGCGAACTGATTTACCGTCATGAAGAt                                                                                                       >  2:8030844/1‑150 (MQ=255)

                                                  aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCg                                                                                                     >  2:9624909/1‑150 (MQ=255)

                                                    tcttttGCTTTTCTGTTTGGGACAGCTAAAATGCCCGCTTTGCTACCAGGGTTTTTTTTTCGTTTTCTTTTCCCACCCGCGAGTCATAAATTGCGCAAGGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTc                                                                                                   <  1:2975301/147‑1 (MQ=255)

                                                         tGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccgccg                                                                                              <  2:4267460/150‑1 (MQ=255)

                                                           tttttCTCTTTCGGTCAGCTAAAAGGCCCGCTTTGCTACCAGGTTTTTTTTTTCTTTGTGTTTTCCCACCCTCGATTAATAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                            <  1:712742/149‑1 (MQ=255)

                                                           catttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCAACCTCGAGTCAGAATATGCGCAACCGGTGTCGCAGACTCAAAACAGCGAACAGATTTACCGTCTTGAAGATCGTCCGCCGCt                                                                                            >  1:6509909/3‑150 (MQ=255)

                                                            ttttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAAAAGCGAACAGATTTACCCTCTTGAATATCGTCCGCCGCtt                                                                                           >  1:4667187/1‑150 (MQ=255)

                                                            tgttCTCTTGCGCTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGAGGTCTGTGACCACCCTCGAGTCAGAAAAAGCGCCACCCGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGACGATCCACcgccgcgt                                                                                           >  2:5801278/3‑148 (MQ=255)

                                                              ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                         >  2:9273133/1‑150 (MQ=255)

                                                              ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTcc                                                                                         <  2:1506098/150‑1 (MQ=255)

                                                               tctcTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCTCGGGTTTGTTTTCTTTGTCTGTTCCCACCCTCGAGTCATAAAAGGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCt                                                                                        <  1:5800446/150‑1 (MQ=255)

                                                                ctctTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTc                                                                                       >  2:8822017/1‑150 (MQ=255)

                                                                           aGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                            <  2:7920090/150‑1 (MQ=255)

                                                                              tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgcc                                                                         >  2:326654/1‑150 (MQ=255)

                                                                              tAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCGGTTTgcc                                                                         >  1:5425697/1‑150 (MQ=255)

                                                                                 aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgcc                                                                      <  2:7003539/150‑1 (MQ=255)

                                                                                  aTGCCCTGTTTGCTCCCACGTGATTGTTTTGGTTTTCTGTTTACACCCGCTATTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCt                                                                     <  1:8772110/150‑1 (MQ=255)

                                                                                  aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTAAAAAAAGCGAACAGATTTAACGTCTTGAAGATCGTCCGCCGCGTCCACAAAACCAGTTTgccgccg                                                                     >  1:3380286/1‑149 (MQ=255)

                                                                                     cccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGTTGCGCAGACTCAAAACAGCGAACAGATTTAACGTCTTGAATATCGTCCCCCCCGTCCACAAACCCAGTTTGCCGCCTGTc                                                                  >  1:847610/1‑150 (MQ=255)

                                                                                     cccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTTTTTGCCGCCTGTc                                                                  >  2:6273032/1‑150 (MQ=255)

                                                                                      ccGCTTTGCTACCCGGGTTTTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCATAAAATGCGAACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCa                                                                 <  1:925/150‑1 (MQ=255)

                                                                                      ccGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCa                                                                 <  2:3910339/150‑1 (MQ=255)

                                                                                       cGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAg                                                                >  2:8136516/1‑150 (MQ=255)

                                                                                        gCTTTGCTACCACGGGATTGTTTTCTATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGc                                                               <  2:530141/150‑1 (MQ=255)

                                                                                         cTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCa                                                              <  2:9548592/150‑1 (MQ=255)

                                                                                            tGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATct                                                           <  1:7570870/150‑1 (MQ=255)

                                                                                             gatACCTCGGGTTTGTTTTCTTTGTCTGTTTCCACCCTCGAGTCATAAATTGCCCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctg                                                          <  1:172169/148‑1 (MQ=255)

                                                                                             gCTACCCCGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAAGGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctg                                                          <  1:3092003/150‑1 (MQ=255)

                                                                                             gCTACCACGGGTTTGTTTTCTTTGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctg                                                          <  1:70179/150‑1 (MQ=255)

                                                                                             gCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctg                                                          >  2:4094325/1‑150 (MQ=255)

                                                                                              cTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGTCTTGAAGATAGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                         >  1:5487790/1‑150 (MQ=255)

                                                                                               tACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGAGAAAAGATTTAACGTCTTGAAGATCGTCCGCCGCGTCCAAAAACCCAGTTTGCCGCCAGTCAGCaacagcg                                                        >  1:433307/1‑144 (MQ=255)

                                                                                                aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgctg                                                       <  2:9044279/150‑1 (MQ=255)

                                                                                                aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACACAAAACAGCGAACTGATTTACCGTCTAGAAGATCGTCCGCCGCGTCCACAAAACCTGTTTTCCGCCAGTCAGCATctgctg                                                       >  1:5693321/1‑150 (MQ=255)

                                                                                                 ccACGGTATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                      <  2:4616599/150‑1 (MQ=255)

                                                                                                 ccACGGGATTTTTTTCTCTGTCTGTTCCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                      <  1:263732/150‑1 (MQ=255)

                                                                                                 ccACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                      <  2:8693832/150‑1 (MQ=255)

                                                                                                 ccACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGACAGAAAATGCGCACCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                      <  1:858551/150‑1 (MQ=255)

                                                                                                  caggtgtttGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGAAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGc                                                     <  1:4468340/143‑1 (MQ=255)

                                                                                                    cGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGa                                                   >  2:9483723/1‑150 (MQ=255)

                                                                                                     gggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCGTCCACAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGAt                                                  >  1:1437579/1‑150 (MQ=255)

                                                                                                     gggATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACACAAAACAGCGAACAGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCCCAAAACCGGGTTGCCGCCGGGCAGCCACTGCTGGCgct                                                  >  1:353318/1‑148 (MQ=255)

                                                                                                        aTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTC‑AAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTc                                              <  2:130184/150‑1 (MQ=255)

                                                                                                             tttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTg                                          >  2:4685648/1‑150 (MQ=255)

                                                                                                              ttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGc                                         >  2:3086425/1‑150 (MQ=255)

                                                                                                               tCGATGTCTGTTTCCACCCTCGAGTCAGAAATTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCg                                        <  2:8279372/150‑1 (MQ=255)

                                                                                                                cgctGTCTGTTGCCACCCTCGAGCCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                                       <  2:2666452/147‑1 (MQ=255)

                                                                                                                cGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCgg                                       <  2:8483214/150‑1 (MQ=255)

                                                                                                                cGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGATCGTTGCgg                                       >  2:2808630/1‑150 (MQ=255)

                                                                                                                  aTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAACCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                     >  2:4392627/1‑150 (MQ=255)

                                                                                                                    gTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGAt                                   <  2:8407880/150‑1 (MQ=255)

                                                                                                                       tGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCAc                                <  2:6004617/150‑1 (MQ=255)

                                                                                                                         tttCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGc                              <  2:5420939/150‑1 (MQ=255)

                                                                                                                          ttCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGcc                             >  2:3153039/1‑150 (MQ=255)

                                                                                                                             cACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgc                          <  2:7638369/150‑1 (MQ=255)

                                                                                                                              ccccTCGAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcg                         <  2:368423/149‑1 (MQ=255)

                                                                                                                               cccTCGAGTCATAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAgcgc                        <  2:3380286/150‑1 (MQ=255)

                                                                                                                                 cTCGCGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                      <  1:3860819/150‑1 (MQ=255)

                                                                                                                                    gAGTCATAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTa                   <  1:2372307/150‑1 (MQ=255)

                                                                                                                                       tCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTAGCCGCCTGTCAGCATCTGCTGGCGATGTTCGATGCGGTGATCACGCCCGCGCTATTAATc                >  2:2140922/1‑150 (MQ=255)

                                                                                                                                       tCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCATCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATc                >  2:6099420/1‑150 (MQ=255)

                                                                                                                                         aGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTg              <  1:6251462/150‑1 (MQ=255)

                                                                                                                                          gAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCCCGCCAGCGCTATTAATCTGc             >  2:1864421/1‑150 (MQ=255)

                                                                                                                                            aaaTGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa           <  1:9706696/150‑1 (MQ=255)

                                                                                                                                              aTGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAgg         >  2:6423018/1‑150 (MQ=255)

                                                                                                                                                   cAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTg    >  2:2235696/1‑150 (MQ=255)

                                                                                                                                                   cAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTg    >  2:1239128/1‑150 (MQ=255)

                                                                                                                                                   cAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTg    >  2:5625000/1‑150 (MQ=255)

                                                                                                                                                     aCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTggg  >  2:7414043/1‑150 (MQ=255)

ATATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGCTGGG  >  NC_000913/3828831‑3829129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`