Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,959,501 T→A 11.2% intergenic (+56/+31) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,959,5010TA11.2% 1366.8 / 55.8 477intergenic (+56/+31)ilvC/ppiCketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
Reads supporting (aligned to +/- strand):  ref base T (140/265);  new base A (58/2);  total (206/271)
Fisher's exact test for biased strand distribution p-value = 9.04e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 484 total aligned reads displayed.

GAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCT  >  NC_000913/3959352‑3959650
                                                                                                                                                     |                                                                                                                                                     
gAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCACTCCCACAGGGAGAGGGAGCAAAAACt                                                                                                                                                       >  1:7853601/1‑150 (MQ=255)
  aCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCGCACCCCGACCCTCACCCACCGGGGGCGGGGGAACACACTCa                                                                                                                                                     >  1:7507449/1‑150 (MQ=255)
  aCGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCGGCCCGCTCCCCCCGGGAGAGGGAGGAAACACTcc                                                                                                                                                     >  1:5772233/1‑149 (MQ=255)
   cGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCaa                                                                                                                                                    <  1:3095629/150‑1 (MQ=255)
    gAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTCAGTGCGCGCTGATGCCCTCACCCCCACCCACAACCACAGGGAGAGGGAGAAAAAACTCAAg                                                                                                                                                   >  1:4870435/1‑150 (MQ=39)
    gAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAg                                                                                                                                                   >  1:7211984/1‑150 (MQ=255)
     aaGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAgg                                                                                                                                                  <  1:6294173/150‑1 (MQ=255)
       gCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATTACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGcc                                                                                                                                                <  2:8926456/150‑1 (MQ=255)
        cGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCt                                                                                                                                               <  1:3433174/150‑1 (MQ=255)
         gATTCGCAGCCACGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCtg                                                                                                                                              <  2:922518/150‑2 (MQ=255)
              gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTGACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCTAGGCCTTCTCCt                                                                                                                                         <  2:6532659/150‑1 (MQ=255)
              gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                                                                         <  1:201880/150‑1 (MQ=255)
              gCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCt                                                                                                                                         <  1:6904970/150‑1 (MQ=255)
               cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTg                                                                                                                                        <  2:1408373/150‑1 (MQ=255)
               cAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTGGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTccag                                                                                                                                        <  2:8640766/150‑3 (MQ=255)
                aGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTgg                                                                                                                                       <  2:4892340/150‑1 (MQ=255)
                       cGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTATGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGc                                                                                                                                <  1:4817035/150‑1 (MQ=255)
                       cGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGc                                                                                                                                <  2:7368515/150‑1 (MQ=255)
                         aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCt                                                                                                                              <  1:8746745/150‑1 (MQ=255)
                         aTTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCAGGAGAAGGCCt                                                                                                                              <  2:3768860/150‑1 (MQ=255)
                           tGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTg                                                                                                                            <  1:990899/150‑1 (MQ=255)
                           tGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTg                                                                                                                            <  2:3001859/150‑1 (MQ=255)
                            gAGCAGGTAGGTAGGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCACACCCCCACCCACACCCCCCCGGCGAGGGTGGAAACAAACAAGGCCATCACCAGGCGAAGGccgttc                                                                                                                           >  1:8305389/1‑146 (MQ=255)
                             aGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCt                                                                                                                          <  2:4125419/150‑1 (MQ=255)
                              gCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTa                                                                                                                         <  1:9707724/150‑1 (MQ=255)
                                aggtaggtAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCAGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTAtt                                                                                                                       <  2:1760940/150‑1 (MQ=255)
                                 ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTa                                                                                                                      <  2:2135090/150‑1 (MQ=255)
                                 ggtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTa                                                                                                                      <  2:6365901/150‑1 (MQ=255)
                                  gtaggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGGCCGCACCCCCCCCCAAACCCCCCGGGAGCGGGGGGAAAAAAACAAAGCCCTCTCAGGGGGAAAGCCGGGCTTTTat                                                                                                                     >  1:1948706/1‑149 (MQ=255)
                                     ggtaAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCCACCCACAACCCCACGGGGGGGGAGAAAAAAAACAAGGCCGTCTACAGGAGGAGGGCGTGCGATTAtttt                                                                                                                  >  1:1011020/1‑149 (MQ=255)
                                       taAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCGCAACCCCACCCACACCCACAAGGGGGGGGGGGAAAAACACAAGGCCATAAACAGGAGAAGGCCCTGCTATTAttttgg                                                                                                                >  1:662185/1‑147 (MQ=255)
                                         aGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCCCCACACCCACAGGGCGAGGGTGAAAACACACAAGGCCATATACCGGAGCACGCCGTGCTATTATTTGCGGt                                                                                                              >  2:8246138/1‑150 (MQ=37)
                                          gAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTa                                                                                                             <  1:9311930/150‑1 (MQ=255)
                                           aaaCTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCACACCCCGACCCACACCCACAGGGAGAGGGAGAAAACACACAATGCCTTATACTGGAGAAGGCCGTGCTATTATTTGCGGTaa                                                                                                            >  1:2354544/1‑149 (MQ=38)
                                                   cgGCTATATGACAGAGATGAAACGTATTGCGGTTGCGGGTTCAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCtt                                                                                                    <  2:9444411/150‑1 (MQ=255)
                                                      cTATATGACAGATATGATACGTTTTGCTTTTGCGGTTTAAGTGCGCTCTGATGCCCTCACCCCGACCCTCTACCTCATTGAGAGGGAGTAAACTCTCAAGGGCTTCTACTGTAGAAGGCCTAGCTATTAGTTGCTTTACAGCACCTTAat                                                                                                 <  2:6766357/150‑1 (MQ=255)
                                                      cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                                                                                 >  2:9625194/1‑150 (MQ=255)
                                                      cTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAat                                                                                                 <  1:1240986/150‑1 (MQ=255)
                                                       tataTGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCAAACCCCCACCCACACCCACAGGGGGAGGGAGAAAACACAAAATGCCATCACCTGGAGAAGGGCATGCGAATAGTTTAGGTACCCCACCGTTatg                                                                                                >  1:5626019/1‑150 (MQ=255)
                                                         tatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgat                                                                                              <  1:4424131/150‑1 (MQ=255)
                                                         tatGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgat                                                                                              <  2:1565337/150‑1 (MQ=255)
                                                          atGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAatgatg                                                                                             <  1:6842894/150‑1 (MQ=255)
                                                             aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCCCAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                                                                          <  2:7347234/150‑1 (MQ=255)
                                                             aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                                                                          <  1:3567651/150‑1 (MQ=255)
                                                             aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGa                                                                                          <  1:393465/150‑1 (MQ=255)
                                                             aCAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCATAATGATGTGa                                                                                          >  2:7251501/1‑150 (MQ=255)
                                                               aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATTCCCACACACCCAACCACAACCACAGGGCGAGGGGGAAAAAACACCAGGCCTTCTCCGGGCGAAGGCCTTGCTATTAGTTGCGGTACAGCACCATAATGATGTGata                                                                                        >  2:3069825/1‑150 (MQ=255)
                                                               aGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGAGGCCCTCACCCCCACCCACACCCACACGGAGAGGGTGAAAACACACAAGGCCATATCCTGGAGAAGGCCATTCTAATATTTTCGGTACAGCACCTTAATTATGTGata                                                                                        >  2:7715684/1‑150 (MQ=255)
                                                                 atatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACACCGAAACACACCCCCAGGGAGAGGGAGAAAAAACTCAATGCCTTCTCCTGGAGAAGGCCGTGCTATTATTTGCGGTACAGCAACAAAATGATGTGataac                                                                                      >  2:4775106/1‑148 (MQ=255)
                                                                  tatGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATcc                                                                                     <  1:9593772/150‑1 (MQ=255)
                                                                    tGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGCCCCTCACCCACAGGGGGCGGGCGAAAACACACAAGGCCTTCACCGGGAGACGGCCGTGCTATTAGGTGCGGTACAGCCCCTTAAAGATGTGAGGTCCgc                                                                                   >  2:6878982/1‑149 (MQ=25)
                                                                     gAAACGTATTGCTGTTGCGGGTTATGTGCGCGCTGATGCCCTCAACCCGACCCTCTCCCACAGGGGGAGGGAGAAAACACTCAAGGCCGTCTGCGGGCGTAGGCCGTTCTATTAGTTGCGGTACAGCACCTTAATTAAGTGGAATGCGaa                                                                                  >  2:7641544/1‑150 (MQ=255)
                                                                          gTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTggg                                                                             <  1:5174885/150‑1 (MQ=255)
                                                                              tGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGTGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGtgtg                                                                         <  1:7441112/150‑1 (MQ=255)
                                                                              tGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGtgtg                                                                         <  1:6087974/150‑1 (MQ=255)
                                                                              tGCGGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAACCCCTCCAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGCACTGGGtgtg                                                                         <  2:8076729/150‑1 (MQ=38)
                                                                                cTGTTGCGGGTTAAGTGCGCGCTGATTCCCGCACCCCGACCCTATCACACAGGGAGAGGGAGAAAACACACAAGGCCTTAACCTGGCGAAGGCCTTGCTATTAGTTGCGGTACAGCAACATAAAGATGTGATATCCGAAAAGGGTGTGCa                                                                       >  2:4023481/1‑150 (MQ=37)
                                                                                      cGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGc                                                                 <  1:7157472/150‑1 (MQ=255)
                                                                                      cGGGTTAAGTGCGCGCTGATGCCCACCCCCCCACCCACAACCCAACGGGGAGGGCGAAAACACAAAAATCCATCTCCACGGGACGGCCGATGTATTAGTTGAGGGACAGCACCATAAAGAAGTTATAACCCAACCGGGTGTGCAGCgggg                                                                 >  2:9018127/1‑149 (MQ=255)
                                                                                       gggTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGcc                                                                <  2:1796872/150‑1 (MQ=255)
                                                                                          ttAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGTGGGAGAAAAAACACAAGGCCTTCACCTGGAGTAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGTTGGGGTATCCGCACGGGGTGTGCAGCGGGCCggg                                                             >  2:5151879/1‑149 (MQ=37)
                                                                                             aGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACCCTCAAGGCCGTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCgg                                                          <  2:6285672/150‑1 (MQ=255)
                                                                                                gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc                                                       <  1:8717620/150‑1 (MQ=255)
                                                                                                gcgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTc                                                       <  2:7507449/150‑1 (MQ=255)
                                                                                                 cgcgcTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTcc                                                      <  2:4521619/150‑1 (MQ=255)
                                                                                                      tGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGTGCCGGTCGGCTCCAGTAc                                                 >  2:2611184/1‑150 (MQ=255)
                                                                                                       gATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCATGCAATTAGTTGCGGTACAGCACCTTAATGATGTGATAACCGAACTGGGTGTGCAGCGGGCCGGACGGCTCCAGTAcc                                                >  2:5068845/1‑150 (MQ=255)
                                                                                                       gATGCCCTCACCCCGACCCACTCCCACAGGGAGAGGGAGAAAAAACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTATTTGCGGTACAGCACCATAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTAcc                                                >  2:347336/1‑150 (MQ=255)
                                                                                                          gCCCTCCCCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGa                                             <  1:4226452/150‑1 (MQ=255)
                                                                                                           cccTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACGCAAGGCCTTCTCCTGGAGTAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAGCTTGGGGTGCAGAGGGCCGGTCGGCTCGCGTACCGGAc                                            >  2:5566493/1‑150 (MQ=38)
                                                                                                             cTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCCAGGCCTTCTCCGGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACaa                                          <  1:3351106/150‑1 (MQ=255)
                                                                                                               cACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAaga                                        <  2:3258754/150‑1 (MQ=255)
                                                                                                                  cccGACCCTCTCCCACAGGGAGAGGGTGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaa                                     <  1:8520415/150‑1 (MQ=255)
                                                                                                                  cccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaa                                     <  2:3613199/150‑1 (MQ=255)
                                                                                                                  cccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaa                                     <  1:1780929/150‑1 (MQ=255)
                                                                                                                   ccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaa                                    >  2:4690951/1‑150 (MQ=255)
                                                                                                                   ccGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAGGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGaaa                                    >  2:911930/1‑150 (MQ=255)
                                                                                                                         ctctCCCTGTGGGAGAGGGAGAAAACACTCAAGGCCTTCACCGGGAGAAGGCCTTGCTATTAGATGCGGTACAGCACCTAAATGATGAGATATCAGAAAAGGGTGTGCAGCGGGCCGGTCGCATCCAGTACCGGACAAGAGAAAACCACt                              >  2:1929716/1‑150 (MQ=255)
                                                                                                                             cccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTAt                          <  2:7177791/150‑1 (MQ=255)
                                                                                                                              ccACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATc                         >  1:2883659/1‑150 (MQ=255)
                                                                                                                               cacaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCg                        >  1:6360949/1‑150 (MQ=255)
                                                                                                                                acaGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGa                       >  2:1342625/1‑150 (MQ=255)
                                                                                                                                 gtgggagagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAAAGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGaa                      >  2:5942037/3‑150 (MQ=255)
                                                                                                                                      agagggagaAAACACTCAAGGCCTGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCg                 <  2:2217611/150‑1 (MQ=255)
                                                                                                                                       gagggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCgg                >  1:5940205/1‑150 (MQ=255)
                                                                                                                                        agggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGACAACCACTTTATCGAACGCCGGa               >  1:5486990/1‑150 (MQ=255)
                                                                                                                                        agggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGa               >  2:847778/1‑150 (MQ=255)
                                                                                                                                        agggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGa               >  1:8244278/1‑150 (MQ=255)
                                                                                                                                          ggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAAc             >  1:4746731/1‑150 (MQ=255)
                                                                                                                                          ggagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCATAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAAc             >  2:4272325/1‑150 (MQ=255)
                                                                                                                                           gagaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAAcc            >  1:3612748/1‑150 (MQ=255)
                                                                                                                                            agaAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCa           >  2:2408145/1‑150 (MQ=255)
                                                                                                                                             gaacccacTCAAGGCCGGCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCAt          <  2:3427587/145‑1 (MQ=255)
                                                                                                                                               aaaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCt        <  2:709289/150‑1 (MQ=255)
                                                                                                                                                aaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTg       >  2:5304355/1‑150 (MQ=255)
                                                                                                                                                aaCACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTg       >  1:1558562/1‑150 (MQ=255)
                                                                                                                                                   acTCAAGGCCTTCTCCTGTAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACGGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAACCCACTTTATCGAACGCCGGAACCATCTGAcc    <  2:5735573/150‑1 (MQ=255)
                                                                                                                                                    cTCAAGGCCTTCTCCTGTAGAAGGCCTTGCTTTTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCTGCGGGCCGGTCGGCTCAAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGAccc   <  1:8975586/150‑1 (MQ=255)
                                                                                                                                                     tCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCt  >  2:7887793/1‑150 (MQ=255)
                                                                                                                                                     tCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCt  >  1:2643996/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAACGAAGCGATTCGCAGCCATGCGATTGAGCAGGTAGGTAAGAAACTGCGCGGCTATATGACAGATATGAAACGTATTGCTGTTGCGGGTTAAGTGCGCGCTGATGCCCTCACCCCGACCCTCTCCCACAGGGAGAGGGAGAAAACACTCAAGGCCTTCTCCTGGAGAAGGCCTTGCTATTAGTTGCGGTACAGCACCTTAATGATGTGATATCCGAACTGGGTGTGCAGCGGGCCGGTCGGCTCCAGTACCGGACAAGAGAAAACCACTTTATCGAACGCCGGAACCATCTGACCCT  >  NC_000913/3959352‑3959650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: