Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,312,423 T→A 6.6% D190V (GAT→GTT)  alsR ← d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,312,4230TA 6.6% 1961.3 / 22.8 593D190V (GAT→GTT) alsRd‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR
Reads supporting (aligned to +/- strand):  ref base T (237/299);  new base A (55/2);  total (292/301)
Fisher's exact test for biased strand distribution p-value = 4.18e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 615 total aligned reads displayed.

GTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCC  >  NC_000913/4312274‑4312572
                                                                                                                                                     |                                                                                                                                                     
  gAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACAtct                                                                                                                                                     <  1:5187666/150‑1 (MQ=255)
   aGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACAtctc                                                                                                                                                    <  1:2550491/150‑1 (MQ=255)
      aaaTAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACAGGTTCGCCCGGAATGGGTCACTACCAGCACAACAACtccgt                                                                                                                                                 >  1:3916269/1‑148 (MQ=255)
       aataatATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtccttc                                                                                                                                                <  1:494927/150‑1 (MQ=255)
       aataatATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtccttc                                                                                                                                                >  1:4731579/1‑150 (MQ=255)
        ataatataatCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCtccttcc                                                                                                                                               <  2:1138167/150‑1 (MQ=255)
            tataatCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTa                                                                                                                                           >  2:1578283/1‑150 (MQ=255)
            tataatCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACAACCAGCACAACAACTCCTTACTGTa                                                                                                                                           >  2:3640326/1‑150 (MQ=255)
            tataatCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCCGAATGGGTCACAACCAGCACAACATCTCCATCCTGTa                                                                                                                                           >  2:9231201/1‑150 (MQ=255)
             ataatCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTaa                                                                                                                                          <  1:6113420/150‑1 (MQ=255)
             ataatCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTaa                                                                                                                                          <  2:4682910/150‑1 (MQ=255)
                   ggCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTCGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGa                                                                                                                                    >  2:7270953/1‑150 (MQ=255)
                    gCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGaa                                                                                                                                   <  2:5928547/150‑1 (MQ=255)
                     ccAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCGTTTACAGCACTGGTTCGCCCCGAAACGGTCACCACCAGCACAACAACACCTTCCAGGAACAACGaac                                                                                                                                  >  2:5786317/1‑149 (MQ=255)
                      cAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGc                                                                                                                                 <  2:2986863/150‑1 (MQ=255)
                       aGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCg                                                                                                                                <  2:7389603/150‑1 (MQ=255)
                        gTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGCTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCgg                                                                                                                               <  1:2688163/150‑1 (MQ=255)
                         tttCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGa                                                                                                                              <  2:7005052/150‑1 (MQ=255)
                             gCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACAGGTTCGCCCCGAATGGGTCACAACCAGCACAACATCTCCTTCCTGTAACAACCAAGCGGAcatc                                                                                                                          >  2:2799788/1‑150 (MQ=255)
                               tATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTTCATCAATTGTTGGGCCGGAATGGGTGACTACAAGCACAAAAAATACTTCCTGTAAAAACGAATCGGAcatcat                                                                                                                        >  1:2130616/1‑150 (MQ=255)
                                 tCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGAcatcatca                                                                                                                      >  2:5155568/1‑150 (MQ=255)
                                   ggTGAATGGTAGCTATGGGTTATACAAATACTCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACAGGTTCGCCCGGAAAGGGTCACAACCAGCACAACATCACCGTCCTGTAACAACGAAGCGGACATCATCatg                                                                                                                    >  2:2129023/1‑150 (MQ=255)
                                     tGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCatgat                                                                                                                  <  1:7046691/150‑1 (MQ=255)
                                         tGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGa                                                                                                              <  1:3580739/150‑1 (MQ=255)
                                            tAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCa                                                                                                           >  2:432331/1‑150 (MQ=255)
                                               cTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCa                                                                                                        <  1:8383701/150‑1 (MQ=255)
                                               cTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCa                                                                                                        <  1:37588/150‑1 (MQ=255)
                                                tATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAg                                                                                                       <  1:2350295/150‑1 (MQ=255)
                                                tATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAg                                                                                                       <  2:7478327/150‑1 (MQ=255)
                                                tATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAg                                                                                                       <  1:1436830/150‑1 (MQ=255)
                                                 aTGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTAAATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCAGTAACAACGAAGCGGACAACATCATGCTGTTCGCATCAgg                                                                                                      >  2:3566549/1‑150 (MQ=255)
                                                   gggTTATACAAATTATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGAt                                                                                                    <  1:9212515/150‑1 (MQ=255)
                                                   gggTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGACTGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGAt                                                                                                    >  2:625065/1‑150 (MQ=255)
                                                   gggTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGAt                                                                                                    <  2:6630361/150‑1 (MQ=255)
                                                   gggTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGAt                                                                                                    <  1:1610454/150‑1 (MQ=255)
                                                    ggTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATa                                                                                                   <  2:948861/150‑1 (MQ=255)
                                                    ggTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACAGGTTAGCCCCGAATTGGTCACAACCATCAAAAAAAATCCATACAGTAAAAACGAACCGGACAACAAAAATATGTGAGCAACAGGATa                                                                                                   >  2:4197925/1‑150 (MQ=255)
                                                     gTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCATCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAg                                                                                                  >  2:535040/1‑150 (MQ=255)
                                                      ttATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAgg                                                                                                 <  1:3208072/150‑1 (MQ=255)
                                                        ataCAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTAAATAACAGGTTCGCCCGCAATGGGTCACAACCAGCCCAACATCTACGTCCTGTAAAAAACAAGCGGACAACAAAAAAAGGGTATCATCAGGATAGGcc                                                                                               >  1:1878615/1‑150 (MQ=255)
                                                         taCAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGGGCATCAGGATAGGCCt                                                                                              <  2:397633/150‑1 (MQ=255)
                                                            aaaTAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACAACCAGCACAACATCTCCTTCCAGTAAAAAAGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGAc                                                                                           >  2:1973284/1‑150 (MQ=255)
                                                            aaaTAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACAGGTTATCCCCGAATGGGTCACAACCACCCCAACAACACCATCCTGTAAAAACCAAGCGGACCACAACAAGAAGTGAGCCACAGGATAGGCCGGAc                                                                                           >  1:9498378/1‑150 (MQ=255)
                                                               taatCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGc                                                                                        <  1:7883432/150‑1 (MQ=255)
                                                               taatCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGc                                                                                        <  1:1505777/150‑1 (MQ=255)
                                                               taatCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGc                                                                                        <  1:3042692/150‑1 (MQ=255)
                                                               taatCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACAGGTTCGCCCCGAATGGGTCCCGACCCCCCCAACAACTCCATCCAGTAACCACGAAGCGGACCTCCACAAGATGTGAGCCTCAGGCTAGGCCGGACCGc                                                                                        >  2:3419250/1‑150 (MQ=255)
                                                                  tCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTa                                                                                     <  1:6312903/150‑1 (MQ=255)
                                                                         cccGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAAt                                                                              <  2:3071092/150‑1 (MQ=255)
                                                                         cccGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAAt                                                                              <  2:4516126/150‑1 (MQ=255)
                                                                               cTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCaa                                                                        <  2:1410394/150‑1 (MQ=255)
                                                                               cTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCaa                                                                        <  2:9057680/150‑1 (MQ=255)
                                                                                  ttttGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCACTGCGCAAGaa                                                                     >  2:8526685/1‑150 (MQ=255)
                                                                                     tGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACtt                                                                  <  1:908741/150‑1 (MQ=255)
                                                                                      gCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTtg                                                                 <  1:8090821/150‑1 (MQ=255)
                                                                                       ccAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTtgt                                                                >  2:3268832/1‑150 (MQ=255)
                                                                                       ccAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAAAGAAGCGGACATCATAATGATGTGAGCATCAGGATAGGCCTGACAGCGTAAGCCAATGCGCAAGAACTtgt                                                                >  2:5605625/1‑150 (MQ=255)
                                                                                       ccAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTACTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTtgt                                                                >  2:4631930/1‑150 (MQ=255)
                                                                                         aGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACAACTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGc                                                              <  2:6139875/150‑1 (MQ=255)
                                                                                             cTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTa                                                          <  1:7445489/150‑1 (MQ=255)
                                                                                              tACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGGAGCGGACATCATCATGATGTGAGCATCAGGTTAGGCCGGACCGCGTACGCCAATGCGCAAGAACTTGTGCTGTAc                                                         >  2:8082642/1‑150 (MQ=255)
                                                                                              tACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTAc                                                         >  2:7178353/1‑150 (MQ=255)
                                                                                              tACGGCCGCTTTTACATCACAGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCAAGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTAc                                                         >  2:2199638/1‑150 (MQ=255)
                                                                                                cGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACAt                                                       <  1:5443441/150‑1 (MQ=255)
                                                                                                cGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACAt                                                       <  1:432331/150‑1 (MQ=255)
                                                                                                cGGCCGCTTTTAAAAAACAGGTTAGCCCGGAATGGGTCACTAACAGCACAACAACTACATCCTGTAACAACGAAGCGGACAACATAATGATGTGAGCATCAGGATAGGCCAGACAGCGTAAGCCCAAGCGCAAGAACATGTTCTGTACAt                                                       >  2:938476/1‑150 (MQ=255)
                                                                                                  gCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGTAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCa                                                     >  2:6480362/1‑150 (MQ=255)
                                                                                                     gCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTCCGCCAATGCGCAAGAACTTGTGCTGTACATCAGca                                                  >  2:1469535/1‑150 (MQ=255)
                                                                                                      cTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGcac                                                 >  1:1751006/1‑150 (MQ=255)
                                                                                                       atttACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGcaca                                                <  1:6469439/149‑1 (MQ=255)
                                                                                                        tttACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGcacaa                                               <  1:5633237/150‑2 (MQ=255)
                                                                                                         ttACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGc                                                                                                <  2:8812864/100‑1 (MQ=255)
                                                                                                         ttACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGc                                                                                                >  1:8812864/1‑100 (MQ=255)
                                                                                                         ttACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGa                                              <  1:7184027/150‑1 (MQ=255)
                                                                                                          tACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGAt                                             <  1:7425588/150‑1 (MQ=255)
                                                                                                          tACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGAt                                             <  2:2437212/150‑1 (MQ=255)
                                                                                                           aCATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATa                                            <  1:4821656/150‑1 (MQ=255)
                                                                                                           aCATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATa                                            >  1:9626722/1‑150 (MQ=255)
                                                                                                             aTCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGc                                          >  1:4146894/1‑150 (MQ=255)
                                                                                                                aCTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCAtt                                       >  1:6678114/1‑150 (MQ=255)
                                                                                                                 cTGGTTCGCCCGGAATGGGTCACTACCAGCACAACCTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCAttt                                      <  2:5012444/150‑1 (MQ=255)
                                                                                                                 cTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCAttt                                      >  2:7709950/1‑150 (MQ=255)
                                                                                                                     ttCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTCTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGAtc                                  <  1:8470743/150‑1 (MQ=255)
                                                                                                                        gCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGAtcctc                               <  1:4030314/150‑1 (MQ=255)
                                                                                                                         cccGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGAtcctcc                              >  1:2858280/1‑150 (MQ=255)
                                                                                                                          ccGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCg                             <  2:6893527/150‑1 (MQ=255)
                                                                                                                                   gTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTAc                    >  2:7892764/1‑150 (MQ=255)
                                                                                                                                    tCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCACTGCGCACGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACa                   >  1:1051730/1‑150 (MQ=255)
                                                                                                                                    tCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACa                   >  1:6853790/1‑150 (MQ=255)
                                                                                                                                    tCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACa                   >  2:8231076/1‑150 (MQ=255)
                                                                                                                                    tCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACa                   >  2:3851107/1‑150 (MQ=255)
                                                                                                                                        tACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATc               >  2:1870932/1‑150 (MQ=255)
                                                                                                                                          ccAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATccc             >  2:2672739/1‑150 (MQ=255)
                                                                                                                                          ccAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCACGATGTGAGCGTCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATccc             <  2:652448/150‑1 (MQ=255)
                                                                                                                                              cacaACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTg         >  1:5783307/1‑150 (MQ=255)
                                                                                                                                                 aacaTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCt      >  1:348023/1‑150 (MQ=255)
                                                                                                                                                 aacaTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCt      >  1:4566123/1‑150 (MQ=255)
                                                                                                                                                    aTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACACATCCCGCTGTCTGGc   >  2:6749101/1‑150 (MQ=255)
                                                                                                                                                    aTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGc   <  2:8387646/150‑1 (MQ=255)
                                                                                                                                                    aTCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGc   <  2:4752760/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTGAGCAAATAATATAATCGGCCAGTTTCGCTATCGGTGAATGGTAGCTATGGGTTATACAAATAATCTTTGCCCCGTTCTTTTTTGCCAGTTCTACGGCCGCTTTTACATCACTGGTTCGCCCGGAATGGGTCACTACCAGCACAACATCTCCTTCCTGTAACAACGAAGCGGACATCATCATGATGTGAGCATCAGGATAGGCCTGACAGCGTACGCCAATGCGCAAGAACTTGTGCTGTACATCAGCACAGATAGCATTTGATCCTCCGGCACCGTACAAATCCCGCTGTCTGGCC  >  NC_000913/4312274‑4312572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: