Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 960,223 A→T 5.6% intergenic (+128/‑41) aroA → / → ycaL 5‑enolpyruvylshikimate‑3‑phosphate synthetase/putative peptidase‑related chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913960,2230AT 5.6% 1599.0 / 10.2 469intergenic (+128/‑41)aroA/ycaL5‑enolpyruvylshikimate‑3‑phosphate synthetase/putative peptidase‑related chaperone
Reads supporting (aligned to +/- strand):  ref base A (160/274);  new base T (31/2);  total (193/276)
Fisher's exact test for biased strand distribution p-value = 4.24e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 474 total aligned reads displayed.

GCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATGGCTATCAGCTCCGGTTTAA  >  NC_000913/960074‑960372
                                                                                                                                                      |                                                                                                                                                     
 cgGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGAt                                                                                                                                                      <  1:5429221/150‑1 (MQ=255)
         ccAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGTGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACataaaa                                                                                                                                              >  2:6594557/1‑148 (MQ=255)
           aGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGTTACATAATAtt                                                                                                                                            >  1:7315591/1‑150 (MQ=255)
           aGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATAtt                                                                                                                                            <  1:4859809/150‑1 (MQ=255)
            ggCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGACAGTGTGAGTCATTTTTGTTAATGGAAGCCGCGCGTGTCAAGGTGAGATTTTGATACATAAAAttt                                                                                                                                           >  1:299476/1‑150 (MQ=255)
             gCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTta                                                                                                                                          <  2:7187447/150‑1 (MQ=255)
             gCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTta                                                                                                                                          <  2:307851/150‑1 (MQ=255)
             gCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTta                                                                                                                                          <  2:2197342/150‑1 (MQ=255)
               aGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGTTACATAATATTtata                                                                                                                                        >  2:3623544/1‑150 (MQ=255)
                 ccTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCGTGTCAATGTAAGTTGTTGTTACATAATAATtatata                                                                                                                                      >  1:7652519/1‑150 (MQ=255)
                 ccTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGACCTTGTGAGTGATTTTGTTTAATGGTAGCGTCGCGTGTCAATTTAAGTTTTTTTTAAATAATATTtatata                                                                                                                                      >  1:6968193/1‑150 (MQ=255)
                  cTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTatatat                                                                                                                                     <  2:6958528/150‑1 (MQ=255)
                   tgaatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTAGCTTGTCAATGTAAGTTTTTGATAAAAAATATTTatatatt                                                                                                                                    >  2:2661640/1‑149 (MQ=255)
                   tgaatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCATGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTTTTGATACATAATATTTatatatt                                                                                                                                    >  1:5210073/1‑149 (MQ=255)
                    gaatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGa                                                                                                                                   <  1:4411770/150‑1 (MQ=255)
                    gaatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGa                                                                                                                                   <  1:5204730/150‑1 (MQ=255)
                    gaatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGa                                                                                                                                   <  2:776409/150‑1 (MQ=255)
                    gaatgaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCGTGTGAGTCATTTTGATTAATGGTTGCGTCGCTTGTGCATGTAAGTTGTTTATAAATAATATTTatatattt                                                                                                                                   >  1:100060/1‑148 (MQ=255)
                        gaaCAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGTCCATGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTGAAAGTAAGTTGTTGTTACATAATATTTATATATTATTaa                                                                                                                               >  1:8074807/1‑150 (MQ=255)
                         aacaacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGACCGTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTGAATGGGAGTTGTTTTTACATAATATTTATATATGTTTAAt                                                                                                                              >  1:1184305/1‑150 (MQ=255)
                          acaacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATc                                                                                                                             <  2:2746345/150‑1 (MQ=255)
                            aacGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGACCTTGTGTGTGATTTTGTTTAATGGTAGCGTCGCGTTTGAATGTTAGTTGTTGTTAAAAAATAATTATATATGATTAATaaa                                                                                                                           >  1:4188270/1‑150 (MQ=255)
                             acGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAAc                                                                                                                          <  1:1286318/150‑1 (MQ=255)
                             acGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAAc                                                                                                                          <  2:8385076/150‑1 (MQ=255)
                                     taaataGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGAtt                                                                                                                  <  2:8146129/150‑1 (MQ=255)
                                      aaataGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTAGGACCTTGTGAGTCATTTTTATTAATTGTAGCGTCGCTTGTCAAAGTTAGTTTTTGTTAAATAATATTTATATATTATTAATAAACGGTTGATTc                                                                                                                 >  1:84518/1‑150 (MQ=255)
                                       aataGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTca                                                                                                                <  1:7867456/150‑1 (MQ=255)
                                         taGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTcaca                                                                                                              <  2:4743143/150‑1 (MQ=255)
                                            ccAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCGTGTAAATTTAAGTTGTTGATACATAATAATTATATATGATTAATAAACGGATGATTAACAAga                                                                                                           >  2:8337364/1‑150 (MQ=255)
                                             cAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATgaa                                                                                                          <  2:1797964/150‑1 (MQ=255)
                                             cAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATgaa                                                                                                          <  1:8803606/150‑1 (MQ=255)
                                              aaaTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTGATTTTGATTAATGGTAGCGTCGCTTGTCAATTTTAGTTGTTGTTAAATAATATTTATATATGATTAATCAACGGTTGATTAACATtaat                                                                                                         >  2:8803606/1‑149 (MQ=255)
                                              aaaTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATgaag                                                                                                         <  1:4018006/150‑1 (MQ=255)
                                                aTCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATgaagaa                                                                                                       <  2:7610358/150‑1 (MQ=255)
                                                 tctttcttTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAAt                                                                                                      <  2:5674747/150‑1 (MQ=255)
                                                    ttctttATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACt                                                                                                   <  2:4355995/150‑1 (MQ=255)
                                                     tctttATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTa                                                                                                  <  2:8057634/150‑1 (MQ=255)
                                                      ctttATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTaa                                                                                                 <  1:9207791/150‑1 (MQ=255)
                                                       tttATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTaaa                                                                                                <  2:2111586/150‑1 (MQ=255)
                                                       tttATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATAAACGGATGATTCACATGAAGAATACTaaa                                                                                                >  2:7016555/1‑150 (MQ=255)
                                                           tCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTGAATGGAAGTTGTTGATACATAATATTTATAAATGATTAATAAACGGATGTTTAACATTAAGAATACTAAAATaa                                                                                            >  2:4935024/1‑149 (MQ=255)
                                                            cAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTAct                                                                                           <  2:327275/150‑1 (MQ=255)
                                                                aCGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTActgctg                                                                                       <  2:9019371/150‑1 (MQ=255)
                                                                aCGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTActgctg                                                                                       <  2:5520277/150‑1 (MQ=255)
                                                                aCGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTActgctg                                                                                       <  2:5351873/150‑1 (MQ=255)
                                                                 cGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTgg                                                                                      <  2:206678/150‑1 (MQ=255)
                                                                     ggCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGAt                                                                                  >  2:3833826/1‑150 (MQ=255)
                                                                      gCACATTGTCGGCGTTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAAGGTAAGTTGTTGTTACATAATATTTATAAATGATTAATCAACGGATGATTCAAATGAAGAATACAAAAATACTGCAGGCGAt                                                                                  >  2:6062483/1‑150 (MQ=255)
                                                                      gCACATTGTCGGCGTTTTTTTTCGGACCTTGGGAGTCATTTTGATGAATGGTAGCGTCGCTTGTCAATTTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGAtt                                                                                 <  1:4737749/150‑1 (MQ=255)
                                                                          aTTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGa                                                                             <  2:8629119/150‑1 (MQ=255)
                                                                           ttGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTTTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACAAGAAGAATACTAAATTACTGCTGGCGATTGCGAc                                                                            >  2:1287403/1‑150 (MQ=255)
                                                                             gTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACct                                                                          <  1:6134344/150‑1 (MQ=255)
                                                                                ggCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTg                                                                       <  1:6208392/150‑1 (MQ=255)
                                                                                  cGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATGTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTgca                                                                     <  2:9401074/150‑1 (MQ=255)
                                                                                       ttttttCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCAt                                                                 <  2:3136625/150‑1 (MQ=255)
                                                                                         ttttCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTa                                                               <  1:934427/150‑1 (MQ=255)
                                                                                         ttttCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTa                                                               <  1:9538879/150‑1 (MQ=255)
                                                                                         ttttCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTa                                                               <  2:786176/150‑1 (MQ=255)
                                                                                          tttCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTAc                                                              <  2:9167857/150‑1 (MQ=255)
                                                                                             cGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGa                                                           <  1:5296352/150‑1 (MQ=255)
                                                                                              ggACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGAc                                                          <  1:2125855/150‑1 (MQ=255)
                                                                                               gACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACa                                                         <  1:1919006/150‑1 (MQ=255)
                                                                                               gACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATAAACGGATGATTAACATGAAGAATACTAAAATAATGCTGGCGATTGCGACCTCAGCAGCATTAATGACa                                                         >  1:2333500/1‑150 (MQ=255)
                                                                                                 ccTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAgg                                                       <  2:1566940/150‑1 (MQ=255)
                                                                                                   ttGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGgt                                                     <  1:1371839/150‑1 (MQ=255)
                                                                                                    tgtgAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAAATTAAGTTGTTTATACATAAAATTTATATATGATTAATCAACGGATGATTCAAATTAAGAATAATAAAATAAAGCTGGCGATTTCGACCACTGCAGCATTACTGACAGGgtg                                                    >  1:707140/1‑150 (MQ=255)
                                                                                                     gtgAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTTTTGATACAAAATATTTATATATTATTAATCAAAGGATTATTAACATTAAGAATAAAAAAATAAAGCAGGCGATTGCGACCTCTGCAGCATTACTGACAGGgtgt                                                   >  1:1619776/1‑150 (MQ=255)
                                                                                                     gtgAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATTATTAATCAACGGATGATTCAAATGAAGAATACTAAATTAAAGCTGGCGATTGCGACCACTGCAGCAATACTTACAGGgtgt                                                   >  1:9491034/1‑150 (MQ=255)
                                                                                                     gtgAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGgtgt                                                   <  1:2801281/150‑1 (MQ=255)
                                                                                                         gTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCaaa                                               <  1:5074447/150‑1 (MQ=255)
                                                                                                         gTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCaaa                                               <  2:5218119/150‑1 (MQ=255)
                                                                                                          tCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCaaaa                                              <  1:3560188/150‑1 (MQ=255)
                                                                                                          tCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCaaaa                                              >  2:5281858/1‑150 (MQ=255)
                                                                                                            aTTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATAAATAATATTTATATATGATTAATAAACGGATGATTCACATGAAGAATAATAAATTACTGCTGGCGATTGCGACCTCTGCAGCAATACTGACAGGGTGTCAAAATa                                            >  1:5720485/1‑150 (MQ=255)
                                                                                                             ttttGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATAc                                           <  1:7531599/150‑1 (MQ=255)
                                                                                                             ttttGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATAc                                           <  1:5862273/150‑1 (MQ=255)
                                                                                                             ttttGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATAc                                           <  2:2465428/150‑1 (MQ=255)
                                                                                                             ttttGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATAc                                           <  2:6740924/150‑1 (MQ=255)
                                                                                                             ttttGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATAc                                           >  2:1716416/1‑150 (MQ=255)
                                                                                                               ttGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACAAAAAAATTATAAAATATTAAAAAACCGATGATTCACAAGAAGAATACAAAAAAAAATCAGGCGATTGCGACCACAGCACCAATACAGACAGCGTGTCAAAAAAccc                                         >  1:9058568/1‑150 (MQ=255)
                                                                                                                   ttAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACgg                                     <  1:3943225/150‑1 (MQ=255)
                                                                                                                      aTGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTAt                                  <  2:595315/150‑1 (MQ=255)
                                                                                                                       tGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTAtt                                 <  1:3020394/150‑1 (MQ=255)
                                                                                                                          tAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGAt                              <  1:9009744/150‑1 (MQ=255)
                                                                                                                          tAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGAt                              <  2:1095075/150‑1 (MQ=255)
                                                                                                                           aGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATa                             <  1:3292409/150‑1 (MQ=255)
                                                                                                                               tCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCaa                         <  1:4628782/150‑1 (MQ=255)
                                                                                                                                    tGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATgg                    <  2:4188270/150‑1 (MQ=255)
                                                                                                                                     gTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATGGc                   >  2:9639754/1‑150 (MQ=255)
                                                                                                                                     gTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATGGc                   >  2:7465463/1‑150 (MQ=255)
                                                                                                                                     gTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATGGc                   >  1:5489403/1‑150 (MQ=255)
                                                                                                                                      tCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATGGCt                  >  1:6450987/1‑150 (MQ=255)
                                                                                                                                         aTGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATGGCTATc               >  2:5362595/1‑150 (MQ=255)
                                                                                                                                          tGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATGGCTATCa              <  2:299476/150‑1 (MQ=255)
                                                                                                                                                ttgttgATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATGGCTATCAGCTCCg        <  2:5743299/150‑1 (MQ=255)
                                                                                                                                                ttgttgATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATGGCTATCAGCTCCg        <  2:4501133/150‑1 (MQ=255)
                                                                                                                                                  gttgATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATGGCTATCAGCTCCGGt      >  2:3973764/1‑150 (MQ=255)
                                                                                                                                                   ttgATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATGGCTATCAGCTCCGGtt     >  1:248464/1‑150 (MQ=255)
                                                                                                                                                     gATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATGGCTATCAGCTCCGGTTTa   >  1:1080278/1‑150 (MQ=255)
                                                                                                                                                      aTACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATGGCTATCAGCTCCGGTTTaa  >  2:6290752/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCGGATTAGCCAGGCAGCCTGAATGAACAACGGGCAATAAATAGCCAAATCTTTCTTTATCAAAACGTCGGCACATTGTCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGCTTGTCAATGTAAGTTGTTGATACATAATATTTATATATGATTAATCAACGGATGATTCACATGAAGAATACTAAATTACTGCTGGCGATTGCGACCTCTGCAGCATTACTGACAGGGTGTCAAAATACCCACGGTATTGATACCAATATGGCTATCAGCTCCGGTTTAA  >  NC_000913/960074‑960372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: