Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,176 T→A 5.1% noncoding (61/171 nt)
M1M (ATG→TTG) †
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1760TA 5.1% 1050.7 / 7.9 354noncoding (61/171 nt)
M1M (ATG→TTG) †
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base T (128/194);  new base A (24/2);  total (153/201)
Fisher's exact test for biased strand distribution p-value = 9.62e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 357 total aligned reads displayed.

TGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCC  >  NC_000913/1287027‑1287324
                                                                                                                                                     |                                                                                                                                                    
      ttCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTcc                                                                                                                                                >  2:6421048/1‑150 (MQ=255)
          cTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACCCACACACGCAGGCGCGAGAGACGAACCTTGGTCGAATATTCTCATCCTACCCCGc                                                                                                                                            >  1:5210993/1‑150 (MQ=14)
             gagaGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGACGTTTCCAGCCCCAACACACTATCCCATGCCCTAGAGGCGCACCTGGGTCGAAGCATCTCAGACGTCCCCGccgg                                                                                                                                         >  1:8862183/1‑147 (MQ=9)
              agagTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAAAGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                                                                        >  2:2429946/1‑150 (MQ=255)
                agTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTAGAAGCTTCTCATCCATCCCCGCATGGGc                                                                                                                                      >  2:1301194/1‑150 (MQ=255)
                   tCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                                                   <  1:6000234/150‑1 (MQ=255)
                     aGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCACGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCATCTAAACCATCCCCCCAgggggcgaac                                                                                                                                 >  1:4761057/1‑144 (MQ=14)
                        ggcTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAtt                                                                                                                              <  2:8065469/148‑1 (MQ=25)
                         gcTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                                                   <  1:150441/143‑1 (MQ=25)
                         aCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCACTCGCAGGCGCGCGAGTCGTACCATGGTCGAAGCATATAATACTTACCCGCATGGGCAGaaaaatt                                                                                                                             >  2:3738150/1‑145 (MQ=11)
                           ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACCCTCAAAACCGGGCCCTCGAGTCGAACCGTGGTCCAACCTTAAAAACCATCCCCCCAAGGGCAGCATATTTaa                                                                                                                           >  1:8025986/1‑150 (MQ=2)
                             ttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAATCTTATCAACCATCCCCCCAAGGGCAGAAAATTTaaat                                                                                                                         >  2:8262271/1‑148 (MQ=11)
                             ttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAtt                                                                                                                         <  2:1610232/150‑1 (MQ=39)
                             ttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCAGGCGCTCGAGTCGAACCTTGGTCCAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAtt                                                                                                                         >  2:3453916/1‑150 (MQ=255)
                               gAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACCCACACTCGCGGGCGCGCGAGTCGAAACTTTGTAGAAGCATATCATACATAACCCCAAGGGCAGAAAAATTAATTga                                                                                                                       >  1:8739954/1‑149 (MQ=2)
                                aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTTGCGCACGACTCGAACCTTTGTCGAAGAGTAGCATCCATCCCCCCAAGGGCCGAAAATTTAAATGCg                                                                                                                      >  1:8313080/1‑150 (MQ=11)
                                  aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGa                                                                                                                    <  1:5711772/150‑1 (MQ=39)
                                         gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTg                                                                                                             <  2:8861092/150‑1 (MQ=38)
                                                 ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGTAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTc                                                                                                     >  2:4954005/1‑150 (MQ=255)
                                                  gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCAAACTTCCCCGCATGGGCCGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                                                    >  2:7776785/1‑150 (MQ=255)
                                                   aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCCGGCGCTCGAGGCGAACCATGGTCGAAGCTTCTCATCCTTCCCCGCCGGGGCAGGATATTTAATTGCGGGTTCGGTGGGAAGTTCAg                                                                                                   >  2:4471124/1‑150 (MQ=14)
                                                    aGGAGTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgg                                                                                                  <  1:531660/150‑1 (MQ=255)
                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                                                >  2:2277773/1‑150 (MQ=255)
                                                       aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGAc                                                                                               <  1:2700996/150‑1 (MQ=255)
                                                        ttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACt                                                                                              <  2:531646/150‑1 (MQ=255)
                                                         tACTCAGCGCGGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtt                                                                                             <  1:3821250/150‑1 (MQ=255)
                                                          aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACttt                                                                                            <  1:5329286/150‑1 (MQ=255)
                                                            tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCGCTCTCGCTGGCGCGCGAGGCGCACCGTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGCATATGTAATTGCGGCTTCGTTGGGCAGGTCAGGGGATTTTg                                                                                          >  2:845476/1‑150 (MQ=14)
                                                             cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGTCTCCGCTCTCTCGCTGGCGCTCGAGTCGATCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGa                                                                                         <  1:7860825/150‑1 (MQ=255)
                                                              aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                                                        >  2:6102010/1‑150 (MQ=255)
                                                              aGCGCCGCGCGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCGGGCGCTCGAGTCGACCCCTGGTCGAAGCTCCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                                                        <  2:5734663/150‑1 (MQ=17)
                                                               gcgcTGCGCGCCTCGCCCTTCGGGTCGTTGCCTGCGGCTACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGATGCTTCTCAACCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                                                       <  2:7227157/150‑1 (MQ=255)
                                                                       cgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCCGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggt                                                                               <  1:4471124/150‑1 (MQ=255)
                                                                        gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGGCGAAGCGTCTCATCCGTCCCCGCCTGGGCCGAATCGTTAATTGCGGCTTCGGGGGGACGGTCAGGGCCTGTGGACAGTGAtgggg                                                                              >  2:439017/1‑148 (MQ=14)
                                                                          ttCGCCCTTCGGGTCGTTGCCGGCGGCAACCCTCTCACGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGCATCTTTAATTGCGGATTCGGTGGGCAGTTCAGGGACTTTTGAAAGGGGtgggggg                                                                            >  2:1088089/1‑149 (MQ=14)
                                                                           tCGCCCTTCGGGTCGTTGCCAGCGTCACCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                                                           <  1:672770/150‑1 (MQ=255)
                                                                            cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGATCCTTGGTCTAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                                                          <  1:6800172/150‑1 (MQ=255)
                                                                            cGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                                                          <  1:7341813/150‑1 (MQ=255)
                                                                              cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                                                             <  1:4079300/97‑1 (MQ=37)
                                                                               ccTTCGGGTCGTTGCCTGCGGCAACCCTCTCACGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAAATTTAAATGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                                                                       >  2:2099522/1‑150 (MQ=255)
                                                                                 ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGTCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                                                     <  1:9383266/150‑1 (MQ=255)
                                                                                 ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGTAGCTTCTCATCCTTCCCCGGATGTGCTGAATATTTTATTGCGGATTCGTTGGGTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                                                     <  1:6926823/150‑1 (MQ=14)
                                                                                 gtgtggTCGTGGGCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                                                     <  1:4963263/146‑1 (MQ=14)
                                                                                   gagagcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAgg                                                                   >  2:5483736/6‑150 (MQ=255)
                                                                                    gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                                                  <  1:8359378/150‑1 (MQ=255)
                                                                                      agcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGCAGGAtt                                                                >  2:5525430/3‑150 (MQ=255)
                                                                                      agcGTTGCCGCAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                                                >  2:2545874/3‑150 (MQ=255)
                                                                                       tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                                                               >  2:751483/1‑150 (MQ=255)
                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTg                                                                                                                        >  2:4832987/1‑91 (MQ=40)
                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTg                                                                                                                        <  1:4833344/91‑1 (MQ=18)
                                                                                          ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                                                            <  1:890484/150‑1 (MQ=255)
                                                                                           tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                                                             >  2:8610941/1‑84 (MQ=38)
                                                                                             ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGCCGTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCt                                                         >  2:2341232/1‑150 (MQ=255)
                                                                                              cagCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGACCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGTATATTTTTTTGCGGATTCGTTGGGTAGTTCAGTGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt                                                        <  1:3695442/148‑1 (MQ=17)
                                                                                               tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                                                       <  1:7772287/150‑1 (MQ=255)
                                                                                               tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                                                       <  1:2538515/150‑1 (MQ=255)
                                                                                               tGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                                                 >  2:1787240/1‑124 (MQ=255)
                                                                                               tGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                                                 <  1:1787240/124‑1 (MQ=255)
                                                                                               tGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                                                 >  2:621105/1‑124 (MQ=255)
                                                                                               tGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                                                 <  1:621105/124‑1 (MQ=255)
                                                                                                gCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCg                                                      <  2:6947382/150‑1 (MQ=255)
                                                                                                gCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGAGGCGTTGTGAAGTTCAGGGACTGTTGAAAGTGAGGGTGGGGGGGGCAGGATTCGAACCTTCg                                                      >  2:7059700/1‑150 (MQ=17)
                                                                                                 cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAACTTGGTCGAAGCTTATCATCCATCCCCCCAGGGGCCGAAAATTTAATTTCGGATTCGTTGGGAAGTTCAGGGACTTTTTAAAGTGATGGTGGTGGGGGAAGGATTCGAAccaaaga                                                     >  1:9526026/1‑145 (MQ=17)
                                                                                                   gCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                                                     <  1:6977549/132‑1 (MQ=255)
                                                                                                     aaCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTGATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGTAAGTTCAGGGACTTTTGTAAGTGATGGTGGTGGGGGATGGATTCGAACCTTCGAAGTc                                                 <  1:8995179/150‑1 (MQ=255)
                                                                                                     aaCGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                                                    >  2:3836713/1‑99 (MQ=255)
                                                                                                     aaCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAg                                                                                                        >  2:5848245/1‑95 (MQ=255)
                                                                                                      aCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCg                                                <  1:2277773/150‑1 (MQ=255)
                                                                                                       cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGa                                               <  1:1413111/150‑1 (MQ=255)
                                                                                                        gccggtgcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                                              <  2:9516489/143‑1 (MQ=255)
                                                                                                        gccggtgcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAt                                              <  2:1240073/143‑1 (MQ=255)
                                                                                                        gCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                                                       <  2:9463982/77‑1 (MQ=255)
                                                                                                          tctctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAGATTTAATTTCGCATTCGTTGGGAAGTTGAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAGTCGAACCGTCGAAGTCGAAGa                                            >  2:3544264/1‑150 (MQ=18)
                                                                                                            tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACg                                          <  2:3324979/150‑1 (MQ=255)
                                                                                                              tcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg                                                                                                                      <  1:118612/72‑1 (MQ=21)
                                                                                                                  tGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAt                                    >  2:6797985/1‑150 (MQ=255)
                                                                                                                   ggCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATGCTTACCCGCATGGGCAGAATATTTTATTTCGGTTTGGTTTGGTAGTTGATGGACTTTTGTAAGTGTTGGTTGTGGGGGTAGGATTGGTACCTTAGAATTAGATTTAGGCAGAtt                                   >  1:5042322/1‑150 (MQ=9)
                                                                                                                   ggCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGAAGACGGCAGagt                                   >  2:7584039/1‑148 (MQ=255)
                                                                                                                       cTCGAGTCGAACCTTGCTCGACGCTTCTCATCCTTCCACGCCTGGTCAGAATATTTAATTTCGGATTCGTTGGTCAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa                               <  1:6556355/150‑1 (MQ=21)
                                                                                                                       cTCGAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa                               <  2:8726396/150‑1 (MQ=255)
                                                                                                                       cTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                                                       <  1:2055406/94‑1 (MQ=255)
                                                                                                                          gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc                            <  1:1141598/150‑1 (MQ=255)
                                                                                                                          gAGTCGAACCTTGGTCGAAGCTTCTCAACCATCCCCCCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTAGAAGTCGATGACGGCAGATTTACAGTc                            >  1:9639591/1‑150 (MQ=255)
                                                                                                                            gacGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg                          <  2:9300264/148‑1 (MQ=255)
                                                                                                                            gTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg                          <  1:6421048/150‑1 (MQ=255)
                                                                                                                            gTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTg                          <  1:281252/150‑1 (MQ=255)
                                                                                                                                aaCCTTCGTCGACGCTACTCATCCGTCCCCGCATCGGCAGAATATTTCATGTCGGATTAGTTGGGAAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTcc                      <  1:3428081/150‑1 (MQ=21)
                                                                                                                                 aCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATGGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTccc                     <  1:2348172/150‑1 (MQ=255)
                                                                                                                                        tCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGcc              <  1:8107967/150‑1 (MQ=255)
                                                                                                                                          gAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGc            >  2:2072227/1‑150 (MQ=255)
                                                                                                                                           aaGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCt           <  1:555080/150‑1 (MQ=255)
                                                                                                                                            aGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTc          <  1:6576530/150‑1 (MQ=255)
                                                                                                                                               ttCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCggg       <  2:6690906/150‑1 (MQ=255)
                                                                                                                                                tctcATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACTGACTGCTCACTTATGCCCCACGGGa      >  1:9169817/1‑150 (MQ=21)
                                                                                                                                                 ctcATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGaa     <  1:5463237/150‑1 (MQ=255)
                                                                                                                                                 ctcATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGaa     >  1:7174777/1‑150 (MQ=255)
                                                                                                                                                  tcATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAc    >  2:3172407/1‑150 (MQ=255)
                                                                                                                                                   cATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAcc   <  2:2201987/150‑1 (MQ=255)
                                                                                                                                                   cATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCCGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAcc   >  2:2851846/1‑150 (MQ=255)
                                                                                                                                                    aTCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAAccc  >  1:5252285/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTCTGCTCCCTTTGGCCGCTCGGGAACCC  >  NC_000913/1287027‑1287324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: