Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 180,011 C→A 5.6% P259T (CCA→ACA)  dgt → deoxyguanosine triphosphate triphosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913180,0110CA 5.6% 1915.8 / 12.1 612P259T (CCA→ACA) dgtdeoxyguanosine triphosphate triphosphohydrolase
Reads supporting (aligned to +/- strand):  ref base C (174/371);  new base A (59/4);  total (235/377)
Fisher's exact test for biased strand distribution p-value = 2.07e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 627 total aligned reads displayed.

TTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTT  >  NC_000913/179862‑180159
                                                                                                                                                     |                                                                                                                                                    
 tttAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTcc                                                                                                                                                     <  2:7620058/150‑1 (MQ=255)
 tttAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTcc                                                                                                                                                     <  2:7836610/150‑1 (MQ=255)
 tttAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTcc                                                                                                                                                     <  1:2250916/150‑1 (MQ=255)
 tttAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTcc                                                                                                                                                     <  2:8970032/150‑1 (MQ=255)
  ttAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCa                                                                                                                                                    <  2:2526261/150‑1 (MQ=255)
   tAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCAt                                                                                                                                                   <  1:8815008/150‑1 (MQ=255)
      aaTATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTaa                                                                                                                                                <  2:4687046/150‑1 (MQ=255)
        tataCCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACg                                                                                                                                              >  1:8044018/1‑150 (MQ=255)
          tacaggTCCGGCGTGGTGGCTTGGCGAAACGACTGAGAAACATCACTATTTAATGTAATAGCCTGGTTTTTATATTTCTGTAGAAGCCTATTTTGGCAGGTTGAGTAAAGAACTTTATTTGGCGCTTTACAGTCGTTTTGCATTAAAGTg                                                                                                                                            <  2:8651475/145‑1 (MQ=255)
            cccGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTGTTCCATTAAAGTGGa                                                                                                                                          >  1:896752/1‑150 (MQ=255)
             ccGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGAt                                                                                                                                         <  2:6248921/150‑1 (MQ=255)
             ccGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGAt                                                                                                                                         <  1:789200/150‑1 (MQ=255)
             ccGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTGTTCCATTAACGTggct                                                                                                                                         >  1:6217631/1‑148 (MQ=255)
                tCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTAt                                                                                                                                      <  1:6480468/150‑1 (MQ=255)
                tCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTAt                                                                                                                                      >  1:7423546/1‑150 (MQ=255)
                tCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTAt                                                                                                                                      <  1:4985838/150‑1 (MQ=255)
                 ccGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATg                                                                                                                                     <  2:3459298/150‑1 (MQ=255)
                 ccGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATg                                                                                                                                     <  1:6266647/150‑1 (MQ=255)
                  cGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCCTTAACGTGGGTTATgg                                                                                                                                    >  1:2861962/1‑150 (MQ=255)
                    gCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGaa                                                                                                                                  >  1:817074/1‑150 (MQ=255)
                    gCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGaa                                                                                                                                  <  1:3584279/150‑1 (MQ=255)
                    gCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGaa                                                                                                                                  <  2:6989038/150‑1 (MQ=255)
                      gtggtgGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAAAGTGGTTTATGGAAGc                                                                                                                                >  1:2419117/1‑150 (MQ=255)
                          tggcgtggcgAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGcc                                                                                                                            <  2:815988/150‑1 (MQ=255)
                            gcgtggcgAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCcga                                                                                                                          <  2:6081366/150‑1 (MQ=255)
                              gtggcgAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCcgacg                                                                                                                        >  2:9223919/1‑150 (MQ=255)
                              gtggcgAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAAAGTGGATTATTGAATCTTacgacg                                                                                                                        >  1:5671663/1‑150 (MQ=255)
                               tggcgAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCcgacga                                                                                                                       <  1:8486346/150‑1 (MQ=255)
                               tggcgAAACGCCTGAGACACATAACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCcgacga                                                                                                                       <  2:8759553/150‑1 (MQ=255)
                                 gcgAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACa                                                                                                                     <  2:4435704/150‑1 (MQ=255)
                                     aaCGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACAtctc                                                                                                                 <  2:1173371/150‑1 (MQ=255)
                                     aaCGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACAtctc                                                                                                                 <  2:2165101/150‑1 (MQ=255)
                                      aCGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTcc                                                                                                                <  1:3036545/150‑1 (MQ=255)
                                       cGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCt                                                                                                               <  2:235213/150‑1 (MQ=255)
                                        gCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTa                                                                                                              <  1:3175835/150‑1 (MQ=255)
                                        gCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCAGTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTa                                                                                                              >  2:4521553/1‑150 (MQ=255)
                                         ccTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTAt                                                                                                             >  2:3656114/1‑150 (MQ=255)
                                           tGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATtg                                                                                                           >  1:2781997/1‑150 (MQ=255)
                                           tGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAAAGTGGTTTATGGAAGCTGCCGACGACATCTCCTATtg                                                                                                           >  2:2004789/1‑150 (MQ=255)
                                                cacaTCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTgg                                                                                                      <  1:528283/150‑1 (MQ=255)
                                                 acaTCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGc                                                                                                     <  1:4236671/150‑1 (MQ=255)
                                                 acaTCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGc                                                                                                     <  2:2962930/150‑1 (MQ=255)
                                                 acaTCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTCCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGc                                                                                                     <  2:5841652/150‑1 (MQ=255)
                                                      aCTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGAcc                                                                                                <  2:3072304/150‑1 (MQ=255)
                                                      aCTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGAcc                                                                                                >  1:555948/1‑150 (MQ=255)
                                                      aCTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGAcc                                                                                                <  1:3926097/150‑1 (MQ=255)
                                                          tttAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGa                                                                                            <  1:9456198/150‑1 (MQ=255)
                                                          tttAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGa                                                                                            <  2:5326786/150‑1 (MQ=255)
                                                          tttAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTAAGGAAGCAGCCGACGACATCTCCTATTGTGTGGCCGAACGTGa                                                                                            >  2:4255487/1‑150 (MQ=255)
                                                           ttAATGAAAAAGCCGGGTTATTATCTTTCTGCTGAAGCCTATCTTGCCCGGTTGCGTATAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTTACGTGGATTATGGAAGCTGCCGACGACATCTCCTCTTGTGTGGCAGACCTTGaa                                                                                           <  2:8072310/150‑1 (MQ=255)
                                                               tGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATg                                                                                       <  1:7057715/150‑1 (MQ=255)
                                                                gAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAAAGGGGCTTATGGAAGCTGCCGACGACAACTCCTATTGTGTGGCAGACCGTGAAGATGc                                                                                      >  1:408971/1‑150 (MQ=255)
                                                                 aaaaaGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCCGTAACGGGGATTATGGAAGCTGCCGACGACATCTCCTCGTGTGTGGCAGACCGTGAAGATGCg                                                                                     >  2:7508607/1‑150 (MQ=255)
                                                                    caGCCGGGTTATTATCTTTCTGAAGAAGCCTCTATTGCCCGGTTGCGTAAAGAACATACTTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTa                                                                                  <  2:6892316/149‑1 (MQ=255)
                                                                     aGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTag                                                                                 <  2:2637204/150‑1 (MQ=255)
                                                                      gCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTaga                                                                                <  2:5236638/150‑1 (MQ=255)
                                                                      gCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTaga                                                                                <  1:5940339/150‑1 (MQ=255)
                                                                      gCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTaga                                                                                <  2:5662414/150‑1 (MQ=255)
                                                                      gCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACCGTAACGTGGAGTATGGAAGCTGCCGCCGACAACACCGCAGGGTTGGCCGACCGGGAAGATGCGGGagc                                                                                >  1:926808/1‑149 (MQ=255)
                                                                         gggTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTGTTCCATTAACGTGGATTATGGAAGCAGCCGCCCACAACTCCTATTGTGTGGCAGACCTTGAAGATTCGGTAGAGaa                                                                             >  1:2564847/1‑150 (MQ=255)
                                                                           gTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGaaaa                                                                           <  1:7612580/150‑1 (MQ=255)
                                                                            ttattaTCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAg                                                                          >  2:3869027/1‑150 (MQ=255)
                                                                              attatCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGTTTATTGAAGCTGCCGACGACATGTCCTATTGTGTGGCAGACCTTGAAGATGTGGTAGAGAAAAGaa                                                                        >  2:6358873/1‑150 (MQ=255)
                                                                              attatCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGaa                                                                        <  2:7199204/150‑1 (MQ=255)
                                                                                tatCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGGCATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAata                                                                      <  1:2047415/150‑1 (MQ=255)
                                                                                tatCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGGGGATTATGGAAGCAGCCGACGACATCTCCGATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAata                                                                      >  2:9398724/1‑150 (MQ=255)
                                                                                           agaagCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGa                                                           <  1:8717761/150‑1 (MQ=255)
                                                                                           agaagCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGa                                                           >  2:1033510/1‑150 (MQ=255)
                                                                                           agaagCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGa                                                           <  1:6929000/150‑1 (MQ=255)
                                                                                           agaagCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGa                                                           <  1:3361138/150‑1 (MQ=255)
                                                                                           agaagCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAAAGTGGATTATGGAAGCTGCCGACGACATCTACAATTGTGTGGCAGACCGTGAAGTTGCGGGGGAGTAAAGAATATTTACCGTTgt                                                           >  1:1697245/1‑149 (MQ=255)
                                                                                           agaagCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAAAGTGGATTATTGAAGCTGCCGACGACATATCCAATTGTGTGGCAGACCTTGAAGATTCGGTAGAGAAAAGAATATTTAAAGTTGa                                                           >  1:6573578/1‑150 (MQ=255)
                                                                                            gaagCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGag                                                          <  2:2766857/150‑1 (MQ=255)
                                                                                               gCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAACGTGGATTATTGAATCTGCCGACCACATATCCGATTGTGTGGGAGACCTTGAAGATGGGGTAGTGAAAATAATAATTTACGTTTatcag                                                       >  1:100525/1‑150 (MQ=255)
                                                                                               gCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAAAGTGGATTATGGTAGCTGCCGACGACATATCCGATTGTTTGGCAGACCATGAATATTCGGTAGAGGAAAGAAAATTTAACGTTTagcag                                                       >  1:1858644/1‑150 (MQ=255)
                                                                                                ccTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGagcagc                                                      <  2:1656887/150‑1 (MQ=255)
                                                                                                ccTATATTGACCTTTTGCGTAAAGAACTTAATTTGGAGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCATACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGagcagc                                                      <  1:5200566/150‑1 (MQ=255)
                                                                                                  tataTTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCtt                                                    >  2:4002797/1‑150 (MQ=255)
                                                                                                     atTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGGGGATTATGGTAGCTTCCGACGACATCTCCTATTTTGTGGCAGACCTTGAAGATTCGGTAGAGAAAATAATATTTAACGTTTAGCAGCTTTat                                                 >  2:2903281/1‑150 (MQ=255)
                                                                                                      ttGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTTGCAGACCTTGAATATGAGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTatc                                                >  2:1268659/1‑150 (MQ=255)
                                                                                                      ttGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTatc                                                <  2:9593948/150‑1 (MQ=255)
                                                                                                        gCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGGGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTatcat                                              >  2:1488031/1‑150 (MQ=255)
                                                                                                         cccGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAAAGTGGTTTATTGAAGCTGCCCACGACAAATCCGATTTTGTTGCAGACCGTGAAGATGCGGTAGAGAAAAGAATATTTAACGTTTTGCAGCTTTataaaa                                             >  1:1317068/1‑146 (MQ=255)
                                                                                                          ccGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTTAGCAGCTTTataaaca                                            >  2:7378992/1‑145 (MQ=255)
                                                                                                          ccGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAAAGTGGGTTATGGAAGAAGCCGACGACATCACCAAATTTGTGGCAGAACATGAAGAAGCGGTGTTGAAAAGAAAATTTAAACTTGAGCAGCTTTataaaaa                                            >  1:678232/1‑145 (MQ=255)
                                                                                                           cGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATcatcat                                           <  1:1371286/150‑1 (MQ=255)
                                                                                                            ggTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACAATAAAGTGGATTATGGAAGCAGCCCACCACAACACCAATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAAAAATTAACGTTGAGCAGCTTTATAATAAtt                                          >  1:7249632/1‑150 (MQ=255)
                                                                                                                gCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCa                                      <  1:4454682/150‑1 (MQ=255)
                                                                                                                gCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCa                                      <  2:8881335/150‑1 (MQ=255)
                                                                                                                           ttAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTggg                           <  1:4663160/150‑1 (MQ=255)
                                                                                                                           ttAATTTGGCGCTTTACAGTCGTTTTACATTAACGTGGCTTATGGAAGCTGCCGACCACATCTCCTATTTTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTAACGTTGAGCAGCTTTATAATCAATTTCACGAAGCGTggg                           >  2:1781355/1‑150 (MQ=255)
                                                                                                                                ttGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGGAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGACGCGTGGGGCCAg                      <  2:4772515/150‑1 (MQ=255)
                                                                                                                                 tGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGc                     <  2:6726030/150‑1 (MQ=255)
                                                                                                                                  ggCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCa                    <  2:2436463/150‑1 (MQ=255)
                                                                                                                                        ttACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCGATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAAAATTTACCGTTGAGCAGCTTTATAATCAATTGCACGAAGCGTGGGGCCAGCATGAGaa              >  2:8264688/1‑150 (MQ=255)
                                                                                                                                         tACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGaaa             <  2:3286531/150‑1 (MQ=255)
                                                                                                                                             gTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGtt         <  2:6550709/150‑1 (MQ=255)
                                                                                                                                              tCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTc        >  2:1348686/1‑150 (MQ=255)
                                                                                                                                              tCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTc        >  2:499170/1‑150 (MQ=255)
                                                                                                                                                    tCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCtt  >  2:4169093/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTTTAAAATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTT  >  NC_000913/179862‑180159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑