Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,758 G→A 6.2% intergenic (+24/+24) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7580GA 6.2% 1397.8 / 52.9 418intergenic (+24/+24)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (229/162);  new base A (8/18);  total (238/180)
Fisher's exact test for biased strand distribution p-value = 7.23e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 439 total aligned reads displayed.

GATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGC  >  NC_000913/1864609‑1864907
                                                                                                                                                               |                                                                                                                                                     
 atatGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGt                                                                                                                                                      <  1:528408/150‑1 (MQ=255)
  tatGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTg                                                                                                                                                     <  1:2499644/150‑1 (MQ=255)
  tatGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTg                                                                                                                                                     <  1:8891034/150‑1 (MQ=255)
    tGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCa                                                                                                                                                   <  2:8257008/150‑1 (MQ=255)
     gCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCaa                                                                                                                                                  <  1:4635472/150‑1 (MQ=255)
      ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAAt                                                                                                                                                 <  2:5657544/150‑1 (MQ=255)
      ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAAt                                                                                                                                                 <  2:5413153/150‑1 (MQ=255)
       cGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGTGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATg                                                                                                                                                >  2:7478453/1‑150 (MQ=255)
        ggATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGc                                                                                                                                               >  2:1605633/1‑150 (MQ=255)
            gatgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGATGCATTGCAccc                                                                                                                                           <  1:1910726/150‑1 (MQ=255)
              tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCgg                                                                                                                                         <  1:9276174/150‑1 (MQ=255)
              tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGATGCATTGCACCCgg                                                                                                                                         <  1:4479470/150‑1 (MQ=255)
              tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGATGCATTGCACCCgg                                                                                                                                         <  1:4275020/150‑1 (MQ=255)
              tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGATGCATTGCACCCgg                                                                                                                                         <  2:2013226/150‑1 (MQ=255)
              tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGATGCATTGCACCCgg                                                                                                                                         <  2:5740113/150‑1 (MQ=255)
               ggCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGc                                                                                                                                        >  2:9561535/1‑150 (MQ=255)
                gCTACACAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                       <  2:6083075/150‑1 (MQ=255)
                gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGATGCATTGCACCCGGca                                                                                                                                       <  1:4173540/150‑1 (MQ=255)
                     aaaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACacaa                                                                                                                                  >  1:118123/1‑150 (MQ=255)
                         cATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATc                                                                                                                              >  2:4705952/1‑150 (MQ=255)
                          aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATca                                                                                                                             >  2:3269522/1‑150 (MQ=255)
                          aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATca                                                                                                                             >  1:2700424/1‑150 (MQ=255)
                           tttgtttgTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcac                                                                                                                            >  1:4018976/1‑150 (MQ=255)
                            ttgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcaca                                                                                                                           <  2:8387977/150‑1 (MQ=255)
                               tttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAACACACCa                                                                                                                        >  2:1110113/1‑150 (MQ=255)
                                   tgtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATc                                                                                                                    >  1:3207759/1‑150 (MQ=255)
                                     tAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATccc                                                                                                                  >  1:7399614/1‑149 (MQ=255)
                                         aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCCgcgc                                                                                                              >  1:1317515/1‑150 (MQ=255)
                                         aaCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAgcgc                                                                                                              >  1:8409625/1‑150 (MQ=255)
                                          aCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCa                                                                                                             >  2:4068417/1‑150 (MQ=255)
                                          aCGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGAAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCa                                                                                                             <  2:1946795/150‑1 (MQ=255)
                                               ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATTCATCCGGCACACAACATCACACCATATACAGCGCAGtttt                                                                                                        >  1:36620/1‑150 (MQ=255)
                                               ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                        >  2:8715241/1‑150 (MQ=255)
                                               ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                        >  1:7897725/1‑150 (MQ=255)
                                               ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                        >  1:5558679/1‑150 (MQ=255)
                                               ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCAACCGGCACACAACAGCACACCAAATCCAGCGCAGtttt                                                                                                        >  1:9400516/1‑150 (MQ=255)
                                                    cTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCtt                                                                                                   >  1:6266499/1‑150 (MQ=255)
                                                     ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCttt                                                                                                  >  1:9661557/1‑150 (MQ=255)
                                                     taaataaaCGCAAAACGTCACCAACTACAACCCTGCACATCTGGCGCAACCCATCGGGGTTGCGAAACTTTATTTTCGTTTTT‑‑‑‑‑‑‑‑‑‑TGTTGTGGGCCGGGTGCAATCCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCttt                                                                                                  <  1:6042549/145‑1 (MQ=255)
                                                       cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                >  1:6713763/1‑150 (MQ=255)
                                                         gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                              >  2:2451667/1‑150 (MQ=255)
                                                         gAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGt                                                                                              >  1:2272207/1‑150 (MQ=255)
                                                          aaaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCAACCGGCACACAACAACACACCAAAACCAGCGCAGTTTTTACTTTTGGTg                                                                                             >  2:8946627/1‑150 (MQ=255)
                                                           aaCGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGc                                                                                            >  2:3124317/1‑150 (MQ=255)
                                                              gCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCgg                                                                                         >  2:7765266/1‑150 (MQ=255)
                                                              gCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGAGTTGCGAAACGTTAATATACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCCATGCATCCGGCCCACAACAACCCACCATATCCAGCGCAGTTTTTCCGGGTGGTGCCgg                                                                                         >  2:9302307/1‑150 (MQ=255)
                                                                aaaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGat                                                                                       >  2:7642875/1‑150 (MQ=255)
                                                                    aGTGACCACCGAGAAAGCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTACTTTCCGTTAA‑‑‑‑‑‑‑‑‑‑GGTTGTGTGCCGGGTGCATGGCATCCGGCACACACCATCACCCCATATCCAGCGCAGTTTTGCCTTTTGGTGCCGGAGATGc                                                                                   <  2:8599825/150‑1 (MQ=255)
                                                                          cAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAAGGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGGATGCGGCACACAACATCACACCATATGCAGCGCAGTTTTTGGTTTTGGTGGCGGATATGCCttttg                                                                             >  2:4450436/1‑147 (MQ=255)
                                                                          cAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TTTTTTGTGCCGGGTGCAATGCATACGGCACACAACATCACACCATATCCCGCGCAGATTTACCATTTGGTGCCGGATATGCCATATc                                                                             >  2:3612855/1‑150 (MQ=255)
                                                                          cAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATTCATCCGGCACACAACATCACACCAAATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATc                                                                             >  1:6406362/1‑150 (MQ=255)
                                                                          cAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTGTGGTGCCGGATATGCCTTATc                                                                             >  2:6818137/1‑150 (MQ=255)
                                                                          cAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGAGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTGGTGGTGCCGGATATGCCTTATc                                                                             >  2:8877416/1‑150 (MQ=255)
                                                                           aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATcc                                                                            >  2:6725067/1‑150 (MQ=255)
                                                                           aaaGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCCGCACACAAAATCACACCAAATCCAGCGCAGGTTTTCCTTTTGGTGCCCGATAAGCCTTATcc                                                                            >  1:7123113/1‑150 (MQ=255)
                                                                               agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCa                                                                        <  2:4881359/150‑1 (MQ=255)
                                                                                 aaaCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATa                                                                      <  2:5337651/150‑1 (MQ=255)
                                                                                 aaaCCTGCACATCTGGCGCAATCCATTCGCGTTGATAAACTTTATTTTACGTTAA‑‑‑‑‑‑‑‑‑‑TTTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATa                                                                      <  2:2589408/150‑1 (MQ=255)
                                                                                     cTGCACATCTGGCGCAATCCATTCGCTTTGCGAAACTTTAATTTACTTTAATTTACTTTATTGTTGTGTGCCGGGTGCAATGCAGCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATcc                                                                            <  1:3836774/150‑1 (MQ=255)
                                                                                           aTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGGCGGGTGCAAAGCATCCCGCCCACAACAACACACCAAATACCGCGCCGTTTTTCCGTTTGGTGCCGGCTATTGCGTATGCACCATAGCaaaatacg                                                            >  1:8252596/1‑145 (MQ=255)
                                                                                            tatGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTTCCGGGGGCAATTCATACGGCCCAAAACAACACACCAAATCCAGCCCCCTTTTTACGTGTGGTTCCGGAAATGACTTTTACAGCATAGCTAATTACGc                                                           >  1:52/3‑150 (MQ=255)
                                                                                            tCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACAACAAATACAGCGCCGTTTTTCCGTTTGGTGCCGGATATGCCGTATACAGCATATCGAAATCCGc                                                           >  1:8037129/1‑150 (MQ=255)
                                                                                                gcgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgaa                                                       >  2:1817083/1‑150 (MQ=255)
                                                                                                    aagccATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGaaa                                                   <  2:3479496/147‑1 (MQ=255)
                                                                                                      tCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATCGCTAATTCCGCTGAAGAAAGt                                                 >  2:5955364/1‑150 (MQ=255)
                                                                                                        cATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTc                                               >  2:7262421/1‑150 (MQ=255)
                                                                                                        cATTCGCGGTGCGACACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGGTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTc                                               <  2:3370114/150‑1 (MQ=255)
                                                                                                          ttCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                             >  2:2240602/1‑150 (MQ=255)
                                                                                                          ttCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                             >  2:7991943/1‑150 (MQ=255)
                                                                                                          ttCGCGTTGCGAAACGTTAATTGACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                             <  1:4740379/150‑1 (MQ=255)
                                                                                                                ttGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCaa                                       <  2:3738400/150‑1 (MQ=255)
                                                                                                                 tGCGAAACTTTAATTAACGTTAA‑‑‑‑‑‑‑‑‑‑TCTATAGTCCCGGGTCCAAACCATCCGCCACACAACATCACACCATATCCACCGCAGATTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAg                                      <  1:5898250/150‑1 (MQ=255)
                                                                                                                   cGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGATAATTCCGCTGAAGAAAGTTCGACCTCAAGca                                    <  2:662864/150‑1 (MQ=255)
                                                                                                                     aaaCGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGcaca                                  <  1:1544839/150‑1 (MQ=255)
                                                                                                                     aaaCGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCACAAGcaca                                  >  2:7619077/1‑150 (MQ=255)
                                                                                                                          ttAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGc                             <  2:4361863/150‑1 (MQ=255)
                                                                                                                          ttAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGc                             >  2:9083764/1‑150 (MQ=255)
                                                                                                                             aTTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAtt                          <  1:6755515/150‑1 (MQ=255)
                                                                                                                                  cGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                     <  1:5981965/150‑1 (MQ=255)
                                                                                                                                  cGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttgtt                     <  2:7939349/150‑1 (MQ=255)
                                                                                                                                                  tgttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACAt               <  2:2916177/150‑1 (MQ=255)
                                                                                                                                                   gttgtGTGCCGGGTGCAAAGCATACGGCACACAACATAAAACCATATCCAGCGCAGTTTTTCCTTTTTGTGCCGGATATTCCTTATCCAGCATAGCTAATTACGCTGAAGAAATTTAGACCACAAGCAAAGCCGCAATTTGTTTGACaag              >  2:4205998/1‑148 (MQ=255)
                                                                                                                                                    ttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg             <  2:5040136/150‑1 (MQ=255)
                                                                                                                                                    ttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg             <  1:9138794/150‑1 (MQ=255)
                                                                                                                                                     tgtgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATggg            <  1:957101/150‑1 (MQ=255)
                                                                                                                                                     tgtgtgCCGGGTGCAATGAATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTTGTGCAGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATggg            <  1:103712/150‑1 (MQ=255)
                                                                                                                                                       tgtgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCa          >  1:5604501/1‑150 (MQ=255)
                                                                                                                                                         tgCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAAt        >  2:7102039/1‑150 (MQ=255)
                                                                                                                                                          gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc       <  1:3123471/150‑1 (MQ=255)
                                                                                                                                                          gCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc       <  2:3033409/150‑1 (MQ=255)
                                                                                                                                                          gCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATc       >  2:6521580/1‑150 (MQ=255)
                                                                                                                                                           ccGGGTGCAATGCATCCGGCATACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg      >  2:1242462/1‑150 (MQ=255)
                                                                                                                                                           ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg      >  1:5054698/1‑150 (MQ=255)
                                                                                                                                                           ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg      >  2:1972265/1‑150 (MQ=255)
                                                                                                                                                           ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTGTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg      >  1:1606138/1‑150 (MQ=255)
                                                                                                                                                           ccGGATGCATTGCACCCGGCACACAACATTACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGATAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg      >  1:8590483/1‑150 (MQ=255)
                                                                                                                                                             gggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg    <  1:2151435/150‑1 (MQ=255)
                                                                                                                                                              ggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTgg   <  1:2885885/150‑1 (MQ=255)
                                                                                                                                                               gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc  <  2:1381772/150‑1 (MQ=255)
                                                                                                                                                               gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc  <  2:3974282/150‑1 (MQ=255)
                                                                                                                                                               |                                                                                                                                                     
GATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAA‑‑‑‑‑‑‑‑‑‑TGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGC  >  NC_000913/1864609‑1864907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: