Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 180,018 C→A 5.6% T261K (ACG→AAG)  dgt → deoxyguanosine triphosphate triphosphohydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913180,0180CA 5.6% 2011.9 / 16.8 610T261K (ACG→AAG) dgtdeoxyguanosine triphosphate triphosphohydrolase
Reads supporting (aligned to +/- strand):  ref base C (180/369);  new base A (57/3);  total (238/372)
Fisher's exact test for biased strand distribution p-value = 4.50e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 616 total aligned reads displayed.

ATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTG  >  NC_000913/179869‑180166
                                                                                                                                                     |                                                                                                                                                    
atatACCCGTCCGGCGTGGTGGCGTGGCGTAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAAc                                                                                                                                                      <  1:8814130/150‑1 (MQ=255)
  ataCCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGt                                                                                                                                                    <  2:5725011/150‑1 (MQ=255)
     cccGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTACTTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGa                                                                                                                                                 <  1:1362446/150‑1 (MQ=255)
     cccGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGa                                                                                                                                                 <  1:1879966/150‑1 (MQ=255)
      ccGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGAt                                                                                                                                                <  1:8964681/150‑1 (MQ=255)
      ccGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGAt                                                                                                                                                >  2:2953478/1‑150 (MQ=255)
         tCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTAt                                                                                                                                             <  1:3034386/150‑1 (MQ=255)
         tCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTAt                                                                                                                                             <  1:3890917/150‑1 (MQ=255)
         tCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTAt                                                                                                                                             <  1:4985781/150‑1 (MQ=255)
          ccGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATg                                                                                                                                            <  2:7815322/150‑1 (MQ=255)
          ccGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATg                                                                                                                                            <  1:5453739/150‑1 (MQ=255)
            ggCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGa                                                                                                                                          >  1:1784666/1‑150 (MQ=255)
             gCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGaa                                                                                                                                         <  2:4500684/150‑1 (MQ=255)
             gCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGaa                                                                                                                                         <  1:3584279/150‑1 (MQ=255)
                tggtggCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCt                                                                                                                                      <  2:4434934/150‑1 (MQ=255)
                  gtggcgtggcGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTGTTCCATTAAAGTGGATTATGGAATCTGc                                                                                                                                    >  1:4534159/1‑150 (MQ=255)
                  gtggcgtggcGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTGAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGc                                                                                                                                    >  1:7265607/1‑150 (MQ=255)
                   tggcgtggcgAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGcc                                                                                                                                   <  2:559414/150‑1 (MQ=255)
                     gcgtggcgAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAACGTTGATTATGGGATCTGCcga                                                                                                                                 >  1:4145551/1‑150 (MQ=255)
                      cgtggcgAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCcgac                                                                                                                                <  2:3790596/150‑1 (MQ=255)
                       gtggcgAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGGGGATTATGGAAGCTGCcgacg                                                                                                                               >  1:5671557/1‑150 (MQ=255)
                        tggcgAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCcgacga                                                                                                                              <  1:5280015/150‑1 (MQ=255)
                            gAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACAtc                                                                                                                          <  1:5855788/150‑1 (MQ=255)
                              aaCGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACAtctc                                                                                                                        <  2:1173371/150‑1 (MQ=255)
                              aaCGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACAtctc                                                                                                                        <  2:2165101/150‑1 (MQ=255)
                               aCGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTcc                                                                                                                       <  2:8078504/150‑1 (MQ=255)
                                  ccTGAGGCACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAAAGTGGATTATTGAAGCTGCCGACGAAATCACCAAt                                                                                                                    >  2:2509901/1‑150 (MQ=255)
                                  ccTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTAt                                                                                                                    >  2:8404137/1‑150 (MQ=255)
                                  ccTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTAt                                                                                                                    >  2:3656114/1‑150 (MQ=255)
                                    tGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACAATAAAGTGGATTATTGAAGCTGCCCACCACAACTccgaaag                                                                                                                  >  2:8160851/1‑145 (MQ=255)
                                          acaTCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGc                                                                                                            >  1:1398745/1‑150 (MQ=255)
                                             tCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGa                                                                                                         <  1:2273539/150‑1 (MQ=255)
                                              cACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATAGTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGAc                                                                                                        <  1:2589866/150‑1 (MQ=255)
                                                   tttAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGa                                                                                                   <  1:329810/150‑1 (MQ=255)
                                                    ttAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGaa                                                                                                  <  1:841051/150‑1 (MQ=255)
                                                        tGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGTAGCTGCCGACGACATCGCCGATTGTGTGGCAGACCTTGTAGATg                                                                                              >  1:514321/1‑150 (MQ=255)
                                                         gAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAACGTGGATTATTGAAGCTGCCGACGACATCTCCTAATGTGTGGCAGACCATGAAGATGc                                                                                             >  1:9502693/1‑150 (MQ=255)
                                                         gAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGc                                                                                             >  1:408710/1‑150 (MQ=255)
                                                              aGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTag                                                                                        <  2:2637204/150‑1 (MQ=255)
                                                               gCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTaga                                                                                       <  1:3131853/150‑1 (MQ=255)
                                                               gCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTaga                                                                                       <  1:2823807/150‑1 (MQ=255)
                                                               gCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACCGTAACGTGGAGTATGGAAGCTGCCGCCGACAACACCGCAGGGTTGGCCGACCGGGAAGATGCGGGagc                                                                                       >  1:926808/1‑149 (MQ=255)
                                                                    gTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGaaaa                                                                                  <  2:8888236/150‑1 (MQ=255)
                                                                    gTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGaaaa                                                                                  <  1:6916940/150‑1 (MQ=255)
                                                                    gTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGaaaa                                                                                  <  1:7612580/150‑1 (MQ=255)
                                                                       attatCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGaa                                                                               <  2:6667198/150‑1 (MQ=255)
                                                                       attatCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGaa                                                                               <  2:6527814/150‑1 (MQ=255)
                                                                       attatCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGTGGTAGAGAAAAGaa                                                                               >  2:3004249/1‑150 (MQ=255)
                                                                         tatCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGGGGATTATGGAAGCAGCCGACGACATCTCCGATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAata                                                                             >  2:9398724/1‑150 (MQ=255)
                                                                          atCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAAAGTGGATTATTGTAGCTGCCGACGACAACAACAATTTTTTGTCAGACCATGAAGATGCGGTAGAGAAAAAAaaat                                                                            >  1:6539079/1‑150 (MQ=255)
                                                                           tCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAAAGTGGATTATGGAAGCTTCCGACGACATCTCCTATTGTGTGGCAGAACATGAAGATGCGGTAGAGAAAAGAAAAtt                                                                           >  2:4685736/1‑150 (MQ=255)
                                                                              ttCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTAc                                                                        <  1:7466592/150‑1 (MQ=255)
                                                                              ttCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAACGTGGATTATGTAATCTGCCCACGACAACTCCTATTGTGTGGCAGACCGTGAAGATTCGGTAGAGAAAAGAAAATTTAc                                                                        >  2:3404135/1‑150 (MQ=255)
                                                                                 tGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATTCGGTAGAGAAAAGAATATTTAACGt                                                                     >  1:8687179/1‑150 (MQ=255)
                                                                                 tGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAAAGTGGGTTATTGTAGCTGCCGACGACAACTCCTATTGTGTTGCAGACCTTGAAGATTCGGTAGAGAAAAGAATATTTAACGt                                                                     >  1:6604759/1‑150 (MQ=255)
                                                                                    agaagCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGa                                                                  <  1:2482320/150‑1 (MQ=255)
                                                                                    agaagCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGa                                                                  <  2:5297574/150‑1 (MQ=255)
                                                                                    agaagCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGa                                                                  >  2:1033510/1‑150 (MQ=255)
                                                                                    agaagCCTATATTGCCCGGTTGCGAAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGa                                                                  <  2:7134649/150‑1 (MQ=255)
                                                                                     gaagCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGag                                                                 <  2:2766857/150‑1 (MQ=255)
                                                                                        gCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGagcag                                                              <  1:8149598/150‑1 (MQ=255)
                                                                                        gCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAACGTGGATTATTGAATCTGCCGACCACATATCCGATTGTGTGGGAGACCTTGAAGATGGGGTAGTGAAAATAATAATTTACGTTTatcag                                                              >  1:100525/1‑150 (MQ=255)
                                                                                         ccTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGtgcagc                                                             <  2:8982969/150‑1 (MQ=255)
                                                                                         ccTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGagcagc                                                             <  2:1656900/150‑1 (MQ=255)
                                                                                         ccTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGagcagc                                                             <  2:4214393/150‑1 (MQ=255)
                                                                                         ccTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGagcagc                                                             <  2:6975786/150‑1 (MQ=255)
                                                                                         ccTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGagcagc                                                             <  1:6146812/150‑1 (MQ=255)
                                                                                         ccTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGagcagc                                                             <  2:1800210/150‑1 (MQ=255)
                                                                                          cTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAAAATTTAACGTTGAGCAGCt                                                            >  2:6867080/1‑150 (MQ=255)
                                                                                           tataTTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTACATTAACGTGGATTATTGAAGCTGCCGACCACATCTCCTATTGTGTGGCAGACCGTGAAGATGCGGTAGAGAAAAGAATATTTTCCGTTGTGCAGCtt                                                           >  1:7064058/1‑150 (MQ=255)
                                                                                              atTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGGGGATTATGGTAGCTTCCGACGACATCTCCTATTTTGTGGCAGACCTTGAAGATTCGGTAGAGAAAATAATATTTAACGTTTAGCAGCTTTat                                                        >  2:2903281/1‑150 (MQ=255)
                                                                                               ttGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTTGCAGACCTTGAATATGAGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTatc                                                       >  2:1268659/1‑150 (MQ=255)
                                                                                               ttGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTatc                                                       <  2:9593948/150‑1 (MQ=255)
                                                                                                  cccGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTatcatc                                                    <  2:4112933/150‑1 (MQ=255)
                                                                                                  cccGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTatcatc                                                    <  2:7423546/150‑1 (MQ=255)
                                                                                                         gCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCa                                             <  2:1479233/150‑1 (MQ=255)
                                                                                                          cGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTGCATTAAGGTGGTTTTTGGTAGCTGCCGACGTCATCTCGTATTTTGTGGCAGTCCTTGTAGTTGGGGTTGTGTTAAGTATTTTTTCGGTTGTGGAGCTTTTTGATGATTTggtg                                            >  2:8781695/1‑147 (MQ=255)
                                                                                                                gAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAATCg                                      >  2:6295447/1‑150 (MQ=255)
                                                                                                                 aaCTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGt                                     <  1:6749606/150‑1 (MQ=255)
                                                                                                                    ttAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTTCCGACGACATCTCCTATTGTGTGGCAGACCGTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTTAGCAGCTTTATCATCATTTGCACGAACCGTggg                                  >  2:7300855/1‑150 (MQ=255)
                                                                                                                    ttAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGACGCGTggg                                  <  2:7534273/150‑1 (MQ=255)
                                                                                                                    ttAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTggg                                  <  1:2067209/150‑1 (MQ=255)
                                                                                                                    ttAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTAACGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTggg                                  >  2:6031275/1‑150 (MQ=255)
                                                                                                                         ttGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAg                             <  2:2408660/150‑1 (MQ=255)
                                                                                                                         ttGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGGAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGACGCGTGGGGCCAg                             <  2:4772515/150‑1 (MQ=255)
                                                                                                                            gcgcTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCAt                          <  1:4320771/150‑1 (MQ=255)
                                                                                                                              gcTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATga                        <  2:3922585/150‑1 (MQ=255)
                                                                                                                              gatttACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATga                        <  2:8653439/148‑1 (MQ=255)
                                                                                                                                  tACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGaaa                    <  2:3286531/150‑1 (MQ=255)
                                                                                                                                   tacgtagTTTTTCATTAACGTTGGTTTTTGTATCTTCCGTCGCCAACTCCTTTTTTTTGGCATAACTTTAATTTGGGGTTGATAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAg                   <  1:6239898/144‑1 (MQ=255)
                                                                                                                                   aCAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAg                   <  1:6308603/150‑1 (MQ=255)
                                                                                                                                       tCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTc               >  2:1348686/1‑150 (MQ=255)
                                                                                                                                       tCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTc               >  2:499170/1‑150 (MQ=255)
                                                                                                                                               cATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCtttt       >  2:2343001/1‑150 (MQ=255)
                                                                                                                                               cATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCtttt       >  2:922815/1‑150 (MQ=255)
                                                                                                                                                aTTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTc      >  1:7869607/1‑150 (MQ=255)
                                                                                                                                                aTTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTc      >  2:204592/1‑150 (MQ=255)
                                                                                                                                                  tAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGc    <  1:8037220/150‑1 (MQ=255)
                                                                                                                                                  tAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGc    >  2:6158767/1‑150 (MQ=255)
                                                                                                                                                    aCGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGCAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCtg  <  2:514321/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATATACCCGTCCGGCGTGGTGGCGTGGCGAAACGCCTGAGACACATCACTATTTAATGAAAAAGCCGGGTTATTATCTTTCTGAAGAAGCCTATATTGCCCGGTTGCGTAAAGAACTTAATTTGGCGCTTTACAGTCGTTTTCCATTAACGTGGATTATGGAAGCTGCCGACGACATCTCCTATTGTGTGGCAGACCTTGAAGATGCGGTAGAGAAAAGAATATTTACCGTTGAGCAGCTTTATCATCATTTGCACGAAGCGTGGGGCCAGCATGAGAAAGGTTCGCTCTTTTCGCTG  >  NC_000913/179869‑180166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑