Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,234 C→A 13.0% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2340CA13.0% 1098.8 / 41.3 465intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (170/208);  new base A (47/31);  total (226/239)
Fisher's exact test for biased strand distribution p-value = 1.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 468 total aligned reads displayed.

TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177086‑2177383
                                                                                                                                                    |                                                                                                                                                     
ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                      >  2:873048/1‑150 (MQ=255)
ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                      >  2:8061285/1‑150 (MQ=255)
   gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATACTGTCGTTTGTTTTAGATTTCaaaa                                                                                                                                                   >  2:4997488/1‑150 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTGTGTAGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTGaaaaa                                                                                                                                                  >  1:3447940/1‑149 (MQ=255)
     aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAata                                                                                                                                                 >  2:3630535/1‑150 (MQ=255)
     aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTGTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                                 >  2:654668/1‑150 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                                >  2:9545959/1‑150 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                                <  1:2760991/150‑1 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                                <  1:3215886/150‑1 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                                >  2:2448132/1‑150 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                               <  1:4126459/150‑1 (MQ=255)
        ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                                                                              <  1:4886606/150‑1 (MQ=255)
         gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAt                                                                                                                                             >  1:2191325/1‑150 (MQ=255)
           ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGa                                                                                                                                           >  2:7184649/1‑150 (MQ=255)
             cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                         <  2:4809248/150‑1 (MQ=255)
             cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGaaa                                                                                                                                         >  1:9425411/1‑150 (MQ=255)
              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                        >  2:5612522/1‑150 (MQ=255)
              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                        >  1:5362240/1‑150 (MQ=255)
                ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAatta                                                                                                                                      >  1:4428008/1‑150 (MQ=255)
                ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                      <  1:404642/150‑1 (MQ=255)
                 caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAattat                                                                                                                                     >  2:4434786/1‑150 (MQ=255)
                  accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    <  2:6320269/150‑1 (MQ=255)
                  accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    <  2:2243983/150‑1 (MQ=255)
                  accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    >  1:5982180/1‑150 (MQ=255)
                      cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                <  1:7254357/150‑1 (MQ=255)
                         gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAAATATTTGttttt                                                                                                                             >  2:1935380/1‑150 (MQ=255)
                         gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAAGAAATTATtttttttt                                                                                                                             >  2:945972/1‑150 (MQ=255)
                          cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                            >  2:6257307/1‑150 (MQ=255)
                           aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                           >  2:1530334/1‑150 (MQ=255)
                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAATAAATTATTTTTTTTTaaa                                                                                                                          >  2:3511307/1‑150 (MQ=255)
                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTGTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAATATAATGAAATTATTTGttttttta                                                                                                                          >  1:8333262/1‑147 (MQ=255)
                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTATGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATAAAATGAAAATAATTGTTTTTaaa                                                                                                                          >  1:8774676/1‑150 (MQ=255)
                             cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAAATATTTTTTTTTAAat                                                                                                                         >  2:2556727/1‑150 (MQ=255)
                                ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGAATTCAAAATATAATGAAATTAATTGTTTTTAAATATc                                                                                                                      >  1:3600273/1‑150 (MQ=255)
                                  cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                    <  2:5709715/150‑1 (MQ=255)
                                  cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                    <  1:5622363/150‑1 (MQ=255)
                                  cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                    <  2:2356114/150‑1 (MQ=255)
                                   gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                                   >  2:7470704/1‑150 (MQ=255)
                                   gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                                   <  2:2060906/150‑1 (MQ=255)
                                   gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATagag                                                                                                                   >  1:8020951/1‑150 (MQ=255)
                                       ttGTGAATTTTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTAGTTTGTTTTCGATTTCAAATTATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                               <  2:6652014/150‑1 (MQ=255)
                                        tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTTAATATCGAGATAAc                                                                                                              >  2:9247627/1‑150 (MQ=255)
                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                             <  2:6066493/150‑1 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                           <  1:3666438/150‑1 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGAt                                                                                                           >  2:8406956/1‑150 (MQ=255)
                                            aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                          <  1:2308981/150‑1 (MQ=255)
                                             atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                         <  2:7833014/150‑1 (MQ=255)
                                             atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAATTAAATTATTTTTTTTTAAAAATATAGATAACCATca                                                                                                         >  1:6777280/1‑150 (MQ=255)
                                               atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                       <  2:1717286/150‑1 (MQ=255)
                                                 tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaa                                                                                                     <  1:8584902/150‑1 (MQ=255)
                                                  gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAATTTAATTATTTTTTTTTAAATATAGAGATAAAGATAACaaaa                                                                                                    >  1:6664272/1‑150 (MQ=255)
                                                   aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                   <  1:1423437/150‑1 (MQ=255)
                                                     tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATAACGATCACAAAAACg                                                                                                 >  2:2768322/1‑150 (MQ=255)
                                                     tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATTAAAATATTTTTTTTTAAATATCGAGATTAAGATAACaaaaaag                                                                                                 >  1:5814969/1‑148 (MQ=255)
                                                       ccGGAACCGCATAACCGCCGCGCTGTGCTTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGTTAACGATCACAAAAACGAc                                                                                               <  2:9029669/150‑1 (MQ=255)
                                                       ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                               <  2:1875051/150‑1 (MQ=255)
                                                        cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTATATATAGAGATTACGATCACAAAAAAGACa                                                                                              >  2:5194200/1‑150 (MQ=255)
                                                               gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaa                                                                                       >  2:5470946/1‑150 (MQ=255)
                                                                    aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAAAGACAATATTAAAatta                                                                                  >  2:4356194/1‑150 (MQ=255)
                                                                    aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAGTTCAAAATATAATGAAAAAAATTGTTTTTAAATATCGAGATAACGATCACAAAAACAACAAAATTAAAataa                                                                                  >  2:2892225/1‑148 (MQ=255)
                                                                     ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGAGTTCAAAAAATAATGAAAATATTTGTTTTTAAATATCGAGCTAACCATCACAAAAACGACAAAAAGAAAagtat                                                                                 >  2:9310822/1‑150 (MQ=255)
                                                                      cgccgcGCGGTGCGTTGTTCAGCATCTGCTTGGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                <  2:8945035/150‑1 (MQ=255)
                                                                         cgcgcgGTGCGTTGTACACCCTCTGCTTTGTCGACCCCACGTAAATTTAAAACCCCTGTTTTTTGTTTTCTATTAAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGAAAACTATCACAAACACGACAATATGAAAATTATTCga                                                                             <  1:5202744/150‑1 (MQ=255)
                                                                          gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACTTACATTTTATTATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                            <  2:3639892/150‑1 (MQ=255)
                                                                            gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                          <  2:1452820/150‑1 (MQ=255)
                                                                             cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAAGAAATTATTTTTTTTTAAAAATAGAGATAACGATCACAAAAAACACAATATGAAAAATATAAgaggag                                                                         >  1:3079368/1‑150 (MQ=255)
                                                                                  gtttgtcCAGCTTCTGATTTGACGACACCACGTAATTTTTCATATCCTGTGTTTTGTTTTGTTTTTCAAAATATAATTAATTTATTTTTTTTTAAATTTGTTGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                    <  1:6401011/148‑1 (MQ=255)
                                                                                  ggttgttCAGAATCTGCTTTGTCGAAACCACGTACATTTTCATATCCTGTCGTTTTTTTTCTATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGAAAACTAACAAACACACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                    <  1:654668/149‑1 (MQ=255)
                                                                                  cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAATTCAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATAACGATCACAAAAACCACAAAATGGAAATTATTCGAGGAGTGaaa                                                                    >  2:4713713/1‑150 (MQ=255)
                                                                                       ttCAGCATCTGTTTTGTCGATACCACGTAAATTTTCATATCCTGTCTTTTTTTTTCTATTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCGATATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaa                                                               <  1:1091882/150‑1 (MQ=255)
                                                                                         ctgCATCTGCTTTGTCGATACCACGTAATTTTTCATATCCTGTCGTTTTTTTTCTATTAAAAAATATAATTAATTTTTTTTTTTTTAAATATCTATATAACGAAAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaa                                                             <  1:6975800/148‑1 (MQ=255)
                                                                                         cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCcaaa                                                             >  2:7102200/1‑150 (MQ=255)
                                                                                            cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                          <  2:477305/150‑1 (MQ=255)
                                                                                             aTCTGCTTTGACGATCCCAGGTACTTTTACATCTGCTGGCTTTTGATTTCTTTTTAAAATTAAATTTAATTTTTTTTTTTTTATATATCGTTTTACCGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  1:1570802/150‑1 (MQ=255)
                                                                                               cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                       <  2:3934745/150‑1 (MQ=255)
                                                                                               cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                       <  2:3704678/150‑1 (MQ=255)
                                                                                               cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTGAAAAAATAAATAAAATAATTTTTTTTTAATATATAGATTAAAAAAACAAAAAAAAAAAAATTAAAAAAATTAGAGGTGGGGAAGGaaaaaaaaaag                                                       >  1:136009/1‑147 (MQ=255)
                                                                                                ttcTTTTTCTATACAACTTAATTTTTAATATCCTGTCTTTTGTTTTCTTTTTCAAAATTTATTTAATTTTTTTTTTTTTAATTATCGTGATAACTTTAACAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGAAAAAAAACGGc                                                      <  2:919511/148‑1 (MQ=255)
                                                                                                       tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAAATATTTGTTTTTTAATATCGAGATAAAGATAACAAAAACGACAAAATGAAAATTAATAGAGGAGTGAAAGGCAAAAAAAACGCCTCCCgg                                               >  2:4149146/1‑149 (MQ=255)
                                                                                                        cGATACCACGTACATTTTCATAGCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAt                                              <  2:7045854/150‑1 (MQ=255)
                                                                                                          aaaCCACTTACTTTTTATTAGCGTGTTTTTTGTTTTCTTTTTAAAATTATATTTAATTTTTTTTTTTTTAATTATCTATATAACTACCAAAAAAACGACAATATTAAAATTTTTCGTGGAGTGAACGGCAAAAAAACGGCCTCCCGATAg                                            <  1:1949876/148‑1 (MQ=255)
                                                                                                             ccACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTGTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTGGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGa                                         >  2:3026899/1‑150 (MQ=255)
                                                                                                              cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGACAAAAAAACGGCCTCCCGATGGGGaa                                        <  2:4537138/150‑1 (MQ=255)
                                                                                                                     aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATTAAAATTATTGGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGGAGCCGTAg                                 >  2:5296786/1‑150 (MQ=255)
                                                                                                                           atatCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAATATTTTTTTTTTAAAATACAGATAAACATAAAAAAAAACACAAAATTAAAATTATTAGAGGAGTGTAAGGCAAAAAAAAGGCCTCCCCATAGGGAAGCCGGAGCAAAGt                           >  2:4269769/1‑150 (MQ=255)
                                                                                                                               ccTGTCTTTTGTTTTCGATTACAAAATATAATTAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  1:3800021/150‑1 (MQ=255)
                                                                                                                               ccTGTCGTTTTTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  2:2694647/150‑1 (MQ=255)
                                                                                                                               ccTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  2:3211906/150‑1 (MQ=255)
                                                                                                                               ccTGTCGTTTGATTTCGATATAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  1:9273457/150‑1 (MQ=255)
                                                                                                                                cTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAACGATCAAAAAAACGACAATATGTAAATTATTAGAGGAGTGAAAGGCCAAAAAACGGCCACCCGATAGGGAAGCCGTAGCAACGTGcgcg                      >  2:9098166/1‑150 (MQ=255)
                                                                                                                                cTGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAAAGACAAAATGAAAAATATTCGAGGAGTGAAAGGCAAAAAAACGGCCACCCGATAGGGAAGCCGTAGCACAGTGcgcg                      >  2:6865268/1‑150 (MQ=255)
                                                                                                                                   tCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                   <  1:7606072/150‑1 (MQ=255)
                                                                                                                                     tttttttttCAATTAAAAAATATAATCAAATTATTTTTTTTAAAATATCTATATAACTAACAAAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                 <  1:1667323/149‑1 (MQ=255)
                                                                                                                                        tgtttTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa              <  2:753882/150‑1 (MQ=255)
                                                                                                                                        tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa              >  2:7634961/1‑150 (MQ=255)
                                                                                                                                        tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa              >  2:7783769/1‑150 (MQ=255)
                                                                                                                                         tttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  1:8224985/149‑1 (MQ=255)
                                                                                                                                            ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc          >  2:1075964/1‑150 (MQ=255)
                                                                                                                                             tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg         <  2:6420781/150‑1 (MQ=255)
                                                                                                                                                    cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  1:6207185/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177086‑2177383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: