Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,245 G→T 14.6% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT14.6% 1079.8 / 33.8 463intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (170/200);  new base T (47/40);  total (218/245)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 472 total aligned reads displayed.

TTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177097‑2177394
                                                                                                                                                    |                                                                                                                                                      
 tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                                      >  2:8103679/1‑150 (MQ=255)
 tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                                      <  2:3663260/150‑1 (MQ=255)
  cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                                     <  2:4809248/150‑1 (MQ=255)
   tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAat                                                                                                                                                    >  2:9235715/1‑150 (MQ=255)
     ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATaattaaatta                                                                                                                                                  >  2:5323519/1‑150 (MQ=255)
      caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                                 <  1:8302295/150‑1 (MQ=255)
      caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                                 <  1:319214/150‑1 (MQ=255)
       accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                                <  1:875625/150‑1 (MQ=255)
       accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                                <  2:4541769/150‑1 (MQ=255)
       accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                                <  2:6320486/150‑1 (MQ=255)
        ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                               <  1:6148923/150‑1 (MQ=255)
              gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttttt                                                                                                                                         <  1:5799129/150‑1 (MQ=255)
              gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAAATATTTGttttt                                                                                                                                         >  2:1935380/1‑150 (MQ=255)
              gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAAGAAATTATtttttttt                                                                                                                                         >  2:945972/1‑150 (MQ=255)
               cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                        >  2:6257307/1‑150 (MQ=255)
                aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                                       >  2:1530334/1‑150 (MQ=255)
                     ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                                  <  2:2530701/150‑1 (MQ=255)
                     ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                                  <  2:5650415/150‑1 (MQ=255)
                       cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                                <  2:2356114/150‑1 (MQ=255)
                       cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                                <  1:5622363/150‑1 (MQ=255)
                       cGAGAATGAGAAAAAAGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGAACATTTTCATATCCTGTCGTTTGATTTCGATTACAAAATATAATGAAATTAATTGTTTTTAAATATCga                                                                                                                                <  2:4334488/150‑1 (MQ=255)
                         agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAgaga                                                                                                                              >  1:2595737/1‑150 (MQ=255)
                         agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgaga                                                                                                                              <  2:6445895/150‑1 (MQ=255)
                            ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                                           <  1:8799545/150‑1 (MQ=255)
                            ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                                           <  1:6635909/150‑1 (MQ=255)
                            ttGTGAATATTGAATGCCGCAACCGCAAAACCGCCGCGCTGTGCGTTGTTCAGCAACTGCTTTGTCGATACCACCTACATTTTCATATCCTGTCGATTGTTTTCGATTTCAAAATATAATGAAATTAATTGTTTTTAAATATCGAGATaa                                                                                                                           <  2:3122251/150‑1 (MQ=255)
                             tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTCAAAAAATAATGAAAATATTTGTTTTTAAATATAGAGATAAc                                                                                                                          >  2:4241715/1‑150 (MQ=255)
                                gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGAt                                                                                                                      >  2:8714598/1‑150 (MQ=255)
                                gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                      >  2:3285605/1‑150 (MQ=255)
                                 aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                                     <  2:8307343/150‑1 (MQ=255)
                                  atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                                    <  2:7833014/150‑1 (MQ=255)
                                  atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATTAAATTATTTTTTTTTTAATATAGAGATAACGAtaa                                                                                                                    >  1:3845907/1‑148 (MQ=255)
                                     ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                                 >  2:7522530/1‑150 (MQ=255)
                                        aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                              <  1:6767877/150‑1 (MQ=255)
                                        aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                              <  2:9000257/150‑1 (MQ=255)
                                        aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                              <  1:1423437/150‑1 (MQ=255)
                                        aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                              <  2:1116808/150‑1 (MQ=255)
                                        aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                              <  1:9383259/150‑1 (MQ=255)
                                             cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTATATATAGAGATTACGATCACAAAAAAGACa                                                                                                         >  2:5194200/1‑150 (MQ=255)
                                              ggAACCGCATAACCGCCGCGCTGTGAGATGTTCAGCAGCTGCTATGTCGATACCACGTACATTTTCATATCCAGTCGATTGTTTTGTTTTTAAAAAATTAATCAAATTATTTGATTTTAAAAAAAGAGATAACAGTAACAAAAAGGaaaa                                                                                                        >  1:8045819/1‑150 (MQ=255)
                                                    gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATTTCGAGATAAAGATCACAAAAACGACAAAATGaa                                                                                                  >  2:6094376/1‑150 (MQ=255)
                                                    gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAAAATAATTTAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAAAGACAAAATTaa                                                                                                  >  1:8400798/1‑150 (MQ=255)
                                                          ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGAGTTCAAAAAATAATGAAAATATTTGTTTTTAAATATCGAGCTAACCATCACAAAAACGACAAAAAGAAAagtat                                                                                            >  2:9310822/1‑150 (MQ=255)
                                                           cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                           <  2:1711673/150‑1 (MQ=255)
                                                           cgccgcGCGGTGCGTTGTTCAGCATCTGCTTGGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                           <  2:8945035/150‑1 (MQ=255)
                                                            gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATTTAATGAAAATATTTTTTTTTAAATATAGAGATAACGATCACAAAAAAGAAAAAATGAAAattatta                                                                                          >  2:2170161/1‑149 (MQ=255)
                                                            gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTGTTGGATTTCAAAATATAATGAAATTATTTGTTTGTAAATATAGTGTTGACGAGCCACAAAAAGCCCATATGAAAcgtattg                                                                                          >  1:6445918/1‑149 (MQ=255)
                                                               gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                                       <  2:3639878/150‑1 (MQ=255)
                                                                  cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTAgaggag                                                                                    >  2:7198009/1‑150 (MQ=255)
                                                                       ggttgttCTGCATCTGCTTTGTCGATACCACGTACTTTTTCATATCCTGTGTTTTTTTTTCTATTTCAAATTATATTTAAATTTTTTTTTTTTAAATATCTTGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                               <  1:372047/149‑1 (MQ=255)
                                                                       cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAATTCAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATAACGATCACAAAAACCACAAAATGGAAATTATTCGAGGAGTGaaa                                                                               >  2:4713713/1‑150 (MQ=255)
                                                                        gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAg                                                                              >  2:3557070/1‑150 (MQ=255)
                                                                         ttgttCAGCATCTGCTTTGTCGATCCCCCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                             <  2:7328737/150‑1 (MQ=255)
                                                                            ttCAGCATCTGTTTTGTCGATACCACGTAAATTTTCATATCCTGTCTTTTTTTTTCTATTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCGATATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaa                                                                          <  1:1091882/150‑1 (MQ=255)
                                                                            ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAATTTTTTTTTTTTTAATATAGAGATAAAGAAAACAAAAAACACAATAAGAAAAATATTAGATGGGTGAAAGGcca                                                                          >  1:5221656/1‑148 (MQ=255)
                                                                            ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTATAATTAAAAAAATAAATAAATTAATTTTTTTTAAAAATAGCGATAAACATAAAAAAAAAAAAAAAAAAAAACTTAATAGAGGAGTTAAAGTCaa                                                                          >  1:4056944/1‑150 (MQ=255)
                                                                             tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                                         <  2:2012457/150‑1 (MQ=255)
                                                                             tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAAATATTTTTTTTTAAATATAGAGATAACGAAAACAAAAAGAAAAAAAGAAAAATATTAGTGGGGTGAAAGGCaaaa                                                                        >  1:5380951/1‑150 (MQ=255)
                                                                              ctgCATCTGCTTTGTCGATACCACGTAATTTTTCATATCCTGTCGTTTTTTTTCTATTAAAAAATATAATTAATTTTTTTTTTTTTAAATATCTATATAACGAAAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaa                                                                        <  1:6975800/148‑1 (MQ=255)
                                                                                 cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                     <  2:477305/150‑1 (MQ=255)
                                                                                  aTCTGCTTTGACGATCCCAGGTACTTTTACATCTGCTGGCTTTTGATTTCTTTTTAAAATTAAATTTAATTTTTTTTTTTTTATATATCGTTTTACCGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                    <  1:1570802/150‑1 (MQ=255)
                                                                                   tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAAAAAAATTAAAATAATTTTTTTTTAATAAAGATATAAACAAAACAAAAAACACAAAATTCAAAATATTAGCGGAGGGTAAGGCcaaaaaaac                                                                   >  1:8208465/1‑148 (MQ=255)
                                                                                    cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTGAAAAAATAAATAAAATAATTTTTTTTTAATATATAGATTAAAAAAACAAAAAAAAAAAAATTAAAAAAATTAGAGGTGGGGAAGGaaaaaaaaaag                                                                  >  1:136009/1‑147 (MQ=255)
                                                                                            tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAAATATTTGTTTTTTAATATCGAGATAAAGATAACAAAAACGACAAAATGAAAATTAATAGAGGAGTGAAAGGCAAAAAAAACGCCTCCCgg                                                          >  2:4149146/1‑149 (MQ=255)
                                                                                             cGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAt                                                         <  2:7045894/150‑1 (MQ=255)
                                                                                               aaaCCACTTACTTTTTATTAGCGTGTTTTTTGTTTTCTTTTTAAAATTATATTTAATTTTTTTTTTTTTAATTATCTATATAACTACCAAAAAAACGACAATATTAAAATTTTTCGTGGAGTGAACGGCAAAAAAACGGCCTCCCGATAg                                                       <  1:1949876/148‑1 (MQ=255)
                                                                                               aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                                       <  2:1035511/150‑1 (MQ=255)
                                                                                                  ccACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTGTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTGGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGa                                                    >  2:3026899/1‑150 (MQ=255)
                                                                                                  ccACGTACATTTTCATATCCTGTCGTTTGTTTTAGAATTCAAAAAATAAAGAAAATATTTTTTTTTAAATATAGAGATAACGATCACAAAAAAGACAAAATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAAACGCCTCCCGATAGGGa                                                    >  2:2852697/1‑150 (MQ=255)
                                                                                                      gTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAAAGATCACAAAAAAGACAATATGAAAAATATTAGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                                >  2:259687/1‑150 (MQ=255)
                                                                                                      gTACATTTTCATATCCTGTCGTTTGTTTTGGATTTGAAAATATAATTTAATTATTTTTTTTTTAATTTGTAGTTAACGATATCAAAATCGTCAATTTGTAAATTTTTGGTGGTGTGTTAGGCAAAAATTAGGGCTCGCGATTGGGattgc                                                >  2:1804289/1‑146 (MQ=255)
                                                                                                       tACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATAACAAAAAAGACAAAATTAAAATTATTGGAGGAGTGGAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                                               >  2:4997475/1‑150 (MQ=255)
                                                                                                              tCATATCCTGTCGTTTGTTTCCTATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCTATATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCTTCCCTATCGGGAGGCCGttttttt                                        <  1:6257307/150‑7 (MQ=255)
                                                                                                                   tCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcg                                   <  2:3845907/150‑1 (MQ=255)
                                                                                                                    ccTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGAAAAAATGAAAATTAATCGAGGAGTGAAAGGCAAAAAAACGGCCTACCGAAAGGGAAGCCGAAGCAAAGTgccc                                  >  2:273650/1‑148 (MQ=255)
                                                                                                                     cTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAACGATCAAAAAAACGACAATATGTAAATTATTAGAGGAGTGAAAGGCCAAAAAACGGCCACCCGATAGGGAAGCCGTAGCAACGTGcgcg                                 >  2:9098166/1‑150 (MQ=255)
                                                                                                                        tCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                              <  1:7606072/150‑1 (MQ=255)
                                                                                                                         cGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                             <  2:6583761/150‑1 (MQ=255)
                                                                                                                          tttttttttCAATTAAAAAATATAATCAAATTATTTTTTTTAAAATATCTATATAACTAACAAAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                            <  1:1667323/149‑1 (MQ=255)
                                                                                                                            ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                          <  2:7139143/150‑1 (MQ=255)
                                                                                                                             tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGGTAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                         >  2:7783713/1‑150 (MQ=255)
                                                                                                                              tttttCTATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                        <  2:5779586/149‑1 (MQ=255)
                                                                                                                              gtttTCGATTTCAAAATATAATGAATTTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                        <  2:7629274/150‑1 (MQ=255)
                                                                                                                               ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCACAGTGCGCGTGGTTTTATg                       >  1:3430708/1‑150 (MQ=255)
                                                                                                                                tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCACAGTGCGCGTGTTTTTATGc                      >  2:9569066/1‑150 (MQ=255)
                                                                                                                                 ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     <  2:4439071/150‑1 (MQ=255)
                                                                                                                                 ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  1:5936148/1‑150 (MQ=255)
                                                                                                                                 ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  2:6681596/1‑150 (MQ=255)
                                                                                                                                  tctaTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                    <  2:5485460/147‑1 (MQ=255)
                                                                                                                                  tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                    <  2:6902103/150‑1 (MQ=255)
                                                                                                                                    gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                  >  1:4991462/1‑150 (MQ=255)
                                                                                                                                       ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc               >  1:4417145/1‑150 (MQ=255)
                                                                                                                                         aaaaaTATAATGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             <  1:2120648/149‑1 (MQ=255)
                                                                                                                                           aactataATGAAATTTTTTGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           <  1:3637555/147‑1 (MQ=255)
                                                                                                                                           aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           <  1:2392989/150‑1 (MQ=255)
                                                                                                                                            aaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt          <  2:1372281/150‑1 (MQ=255)
                                                                                                                                             ataaAATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa         <  2:917499/150‑1 (MQ=255)
                                                                                                                                              tataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa        <  1:733944/150‑1 (MQ=255)
                                                                                                                                               ataATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa       <  1:2975162/150‑1 (MQ=255)
                                                                                                                                                    gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc  >  1:9031209/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  NC_000913/2177097‑2177394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: