Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,279 C→A 5.3% intergenic (‑75/+233) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2790CA 5.3% 1336.9 / 7.1 437intergenic (‑75/+233)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (180/217);  new base A (31/8);  total (212/225)
Fisher's exact test for biased strand distribution p-value = 4.02e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 449 total aligned reads displayed.

AATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTC  >  NC_000913/2177130‑2177428
                                                                                                                                                     |                                                                                                                                                     
  tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAAAAATGAAATTATTTGTTTTTAAATATAGAgataacgataac                                                                                                                                                     >  1:1413590/1‑150 (MQ=255)
    ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGGTAAAGATCACaa                                                                                                                                                   >  2:7759378/1‑150 (MQ=255)
    ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTATATTTGAAAAAAAAAATAAAATATTTTTTTTTTAATATAGATATAAAGATAACaa                                                                                                                                                   >  1:6596594/1‑150 (MQ=255)
    ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAAAGATCACaa                                                                                                                                                   >  2:7511600/1‑150 (MQ=255)
    ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAAATAAAATATTTTTTTTTAAATATAGTGATAACCATAACaa                                                                                                                                                   >  1:6902103/1‑150 (MQ=255)
       aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                                <  1:7591539/150‑1 (MQ=255)
       aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                                <  2:7053421/150‑1 (MQ=255)
       aaTGCCGGAACCGCATAACCCCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCGTATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                                <  2:1804204/150‑1 (MQ=255)
        aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAAAGATCACAAAAAc                                                                                                                                               >  1:634910/1‑150 (MQ=255)
          gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                                                             <  2:296269/150‑1 (MQ=255)
           ccGGAACCGCATAACCGCCGCGCTGTGCTTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGTTAACGATCACAAAAACGAc                                                                                                                                            <  2:9029669/150‑1 (MQ=255)
           ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                                                                            <  2:1875252/150‑1 (MQ=255)
            cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTATATATAGAGATTACGATCACAAAAAAGACa                                                                                                                                           >  2:5194200/1‑150 (MQ=255)
             ggAACCGCATAACCGCGGGGCTGTGCTTTGTTCAGCTTCTGCTTTGTCTATACCACGTAATTTTTATTATCGTTTCTTTTTTTTTCGATTTAAAATTATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCAAAAAAACGACaa                                                                                                                                          <  2:4705009/150‑1 (MQ=255)
                  cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGa                                                                                                                                     >  2:9513722/1‑150 (MQ=255)
                   gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATTTCGAGATAAAGATCACAAAAACGACAAAATGaa                                                                                                                                    >  2:6094376/1‑150 (MQ=255)
                   gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaa                                                                                                                                    >  2:5470946/1‑150 (MQ=255)
                     aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTTAATATCGAGATAACGATCACAAAAACGACAAAATGgaaa                                                                                                                                  >  1:1570638/1‑150 (MQ=255)
                     aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAAGGAAATTATTTGTTTTTAAATATCGAGATAACCCTCCCCAAAACCACAAGAGGaaaa                                                                                                                                  >  2:3637555/1‑150 (MQ=255)
                          cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                                                             <  2:6481249/150‑1 (MQ=255)
                          cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATAGCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                                                             <  2:3730539/150‑1 (MQ=255)
                           gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATAACAAAAAAGACAAAATGAAAattatta                                                                                                                            >  2:9232164/1‑149 (MQ=255)
                             cgcgcgGTGCGTTGTACACCCTCTGCTTTGTCGACCCCACGTAAATTTAAAACCCCTGTTTTTTGTTTTCTATTAAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGAAAACTATCACAAACACGACAATATGAAAATTATTCga                                                                                                                          <  1:5202744/150‑1 (MQ=255)
                                 cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAAGAAATTATTTTTTTTTAAAAATAGAGATAACGATCACAAAAAACACAATATGAAAAATATAAgaggag                                                                                                                      >  1:3079368/1‑150 (MQ=255)
                                      ggttgttCTGCATCTGCTTTGTCGATACCACGTACTTTTTCATATCCTGTGTTTTTTTTTCTATTTCAAATTATATTTAAATTTTTTTTTTTTAAATATCTTGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                                 <  1:372047/149‑1 (MQ=255)
                                      cGTTGTTCAGCATCTGCTTTGTCGATACAACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                                 <  2:7962368/150‑1 (MQ=255)
                                        ttgttCAGCATCTGCTTTGTCGATCCCCCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                                                               <  2:7328737/150‑1 (MQ=255)
                                           ttCAGCATCTGTTTTGTCGATACCACGTAAATTTTCATATCCTGTCTTTTTTTTTCTATTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCGATATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaa                                                                                                            <  1:1091882/150‑1 (MQ=255)
                                           ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTATAATTAAAAAAATAAATAAATTAATTTTTTTTAAAAATAGCGATAAACATAAAAAAAAAAAAAAAAAAAAACTTAATAGAGGAGTTAAAGTCaa                                                                                                            >  1:4056944/1‑150 (MQ=255)
                                                 ttCTGTTTTGTGTCTACCACTTAAATTTTCATATCCTGTCTTTTTTTTTCTATTTAAAATTATAATGAATTTATTTGTTTTTAAATATCGATATAACGAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                                      <  1:9616090/149‑1 (MQ=255)
                                                 aTCTGTTTTGTCTATACAACTTAATTTTTCATATCTTGTCGTTTTTTTTTTTTTAAAAAATATAATTAATTTATTTTTTTTTAAATATCTATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                                      <  1:3457875/150‑1 (MQ=255)
                                                 aTCTGCTTTGTCGATACCACGTACATTTTAATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                                      <  2:3651686/150‑1 (MQ=255)
                                                   cTGCTTTGTCGATACCACGTACATTTTCATATCCTTTCGTTTGTTTTCGATATCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                                                                    <  2:1641122/150‑1 (MQ=255)
                                                   cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTGAAAAAATAAATAAAATAATTTTTTTTTAATATATAGATTAAAAAAACAAAAAAAAAAAAATTAAAAAAATTAGAGGTGGGGAAGGaaaaaaaaaag                                                                                                    >  1:136009/1‑147 (MQ=255)
                                                   cTGATTTGTCCAAACCCCTTACATTTTCATATCCTTTGTTTTTTTTTCTTTTTAAAACTATAATTAAATTTTTTTTTTTTAAATTTCTATATAACTATAAAAAAAACGAAAATATGAAAATTATTCGAGGTGTGAAAGGCAAAAAAACgg                                                                                                    <  1:5915906/150‑1 (MQ=255)
                                                      cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCt                                                                                                 >  2:7218780/1‑150 (MQ=255)
                                                        ttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                                                               <  2:2463007/150‑1 (MQ=255)
                                                         tGTCTATACCACTTAAATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATTTAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTccc                                                                                              <  2:5693502/150‑1 (MQ=255)
                                                           tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                                                            <  2:8673794/150‑1 (MQ=255)
                                                           tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGGAAGGCAAAAAAACGGCCTCCCGa                                                                                            >  2:6347389/1‑150 (MQ=255)
                                                           tCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTCAAAAAATAATTTAAATAATTTTTTTTAAAAAAAGATATAAAGATAAAAAAAACGACAAAATGAAAAATATTCTGGGGGTGTAAGGGCAAAAAAAGGCCACCCgc                                                                                            >  2:8966875/1‑149 (MQ=255)
                                                              aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                                                                         <  2:6785235/150‑1 (MQ=255)
                                                                aCCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAAGGAAAATAATTGGTTTTAAATAACGAGCGAACCATCACAAAAACCACCAAATGACAAATAGTCGCGGCGGGCAAGGCCAAAAAACGGCCGccccagacgg                                                                                       >  2:2721017/1‑143 (MQ=255)
                                                                aCCACGTACATTTTCATATCCGGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                                                                       <  2:7812146/150‑1 (MQ=255)
                                                                   aCGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAATATAATGAAATTATTTTTTTTTTAATATAGAGATAAAGATCACAAAAACGACAATATTTAAATTATTAGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAg                                                                                    >  2:4454958/1‑150 (MQ=255)
                                                                    cGTAAATTTTCATATCCTGTCTTTTTTTTTCTATTTAAAAATATAATTAAATTTTTTTTTTTTAAATATCGATATAACTAAAAAAAAAACGACAATATGAAAATTATTCGAGGTGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                                                                   <  1:8054922/150‑1 (MQ=255)
                                                                        cATTTTCATATCCGGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAACAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                               <  2:760884/150‑1 (MQ=255)
                                                                        cATTTACATACCCTGTCGTTTGTTTTCGATTTAAAAATATAATCAAATTTTTTTTTTTTAAATATCGAGATAACTATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                               <  1:4851627/150‑1 (MQ=255)
                                                                         aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATTAAAATTATTGGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGGAGCCGTAg                                                                              >  2:5296786/1‑150 (MQ=255)
                                                                                tatCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                                                                       <  2:9446194/150‑1 (MQ=255)
                                                                                 atCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTTTTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                                                                      <  1:2214139/150‑1 (MQ=255)
                                                                                   ccTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGTGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                                                    <  2:4784678/150‑1 (MQ=255)
                                                                                    cTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATGACAAAAACGACAATATGAAAATTATTGGAGGTGTGTAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTATCAATGttggcg                                                                   >  2:4830727/1‑145 (MQ=255)
                                                                                     tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                                                                  >  2:4286363/1‑150 (MQ=255)
                                                                                     tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                                                                  >  2:914891/1‑150 (MQ=255)
                                                                                        cGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                                                               <  2:6583761/150‑1 (MQ=255)
                                                                                         gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                                                              <  2:3053101/150‑1 (MQ=255)
                                                                                           ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                                                            <  2:7139143/150‑1 (MQ=255)
                                                                                             tttttCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                                                          <  1:6439066/149‑1 (MQ=255)
                                                                                              ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                                                         >  1:681503/1‑150 (MQ=255)
                                                                                                 tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                                                      <  2:6902103/150‑1 (MQ=255)
                                                                                                    aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                                                   <  1:6002412/150‑1 (MQ=255)
                                                                                                       tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg                                                <  1:3285605/150‑1 (MQ=255)
                                                                                                        cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCACAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg                                               >  1:6207445/1‑150 (MQ=255)
                                                                                                        aaaaaTATAATTAAATTATTTGTTTTTAAATATCGATATAACGATAACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg                                               <  1:4386373/149‑1 (MQ=255)
                                                                                                          aactataATGAAATTTTTTGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg                                             <  1:3637555/147‑1 (MQ=255)
                                                                                                          aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg                                             <  1:2392989/150‑1 (MQ=255)
                                                                                                          aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg                                             >  2:472430/1‑150 (MQ=255)
                                                                                                           aaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt                                            >  2:3426179/1‑150 (MQ=255)
                                                                                                             tataCTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa                                          <  1:8391196/150‑1 (MQ=255)
                                                                                                             tataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa                                          <  2:9654953/150‑1 (MQ=255)
                                                                                                             tataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa                                          >  2:1030505/1‑150 (MQ=255)
                                                                                                              ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                         <  1:5622579/150‑1 (MQ=255)
                                                                                                              ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                         <  2:2009841/150‑1 (MQ=255)
                                                                                                                aaTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa                                       >  1:20057/1‑150 (MQ=255)
                                                                                                                   gAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc                                    <  1:8207850/150‑1 (MQ=255)
                                                                                                                   gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc                                    >  1:9031407/1‑150 (MQ=255)
                                                                                                                   gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc                                    >  2:1449776/1‑150 (MQ=255)
                                                                                                                    aaaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACg                                   >  1:2654628/1‑150 (MQ=255)
                                                                                                                    aaaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACg                                   <  1:9251798/150‑1 (MQ=255)
                                                                                                                     aaTTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGt                                  <  2:2796344/150‑1 (MQ=255)
                                                                                                                       ttattTTTTTTTAAATATCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTgg                                <  1:5390512/150‑1 (MQ=255)
                                                                                                                       ttattTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTgg                                <  2:4189054/150‑1 (MQ=255)
                                                                                                                            tttttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTg                           <  2:4158761/148‑1 (MQ=255)
                                                                                                                               ttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTa                        <  2:1080314/150‑1 (MQ=255)
                                                                                                                                   aaaTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa                    >  1:8609037/1‑150 (MQ=255)
                                                                                                                                      tatCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGc                 <  1:8406956/150‑1 (MQ=255)
                                                                                                                                      tatCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGc                 <  2:2596331/150‑1 (MQ=255)
                                                                                                                                       atCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCa                <  2:3215354/150‑1 (MQ=255)
                                                                                                                                       atCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCa                <  2:3982467/150‑1 (MQ=255)
                                                                                                                                       atCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCAGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGct                >  1:2870809/1‑149 (MQ=255)
                                                                                                                                        tCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCAt               >  1:8770542/1‑150 (MQ=255)
                                                                                                                                          gagaTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGa             <  2:9322183/150‑1 (MQ=255)
                                                                                                                                           agaTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGCAAGGCAAACCCACGGCCTCCCGCTCGGGCCGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGAt            >  2:8466307/1‑150 (MQ=255)
                                                                                                                                           agaTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGAt            >  2:1314668/1‑150 (MQ=255)
                                                                                                                                               aaCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGa        >  2:2322230/1‑150 (MQ=255)
                                                                                                                                                 cGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACg      >  2:5018839/1‑150 (MQ=255)
                                                                                                                                                  gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTCTGTAGGCATGATAAGACGc     >  2:8285564/1‑150 (MQ=255)
                                                                                                                                                  gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc     >  1:1665725/1‑150 (MQ=255)
                                                                                                                                                     cacaAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTc  <  1:7243124/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTC  >  NC_000913/2177130‑2177428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: