Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,185,435 G→T 6.8% intergenic (‑134/+89) thiM ← / ← rcnR hydoxyethylthiazole kinase/transcriptional repressor of rcnA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,185,4350GT 6.8% 1571.9 / 7.9 499intergenic (‑134/+89)thiM/rcnRhydoxyethylthiazole kinase/transcriptional repressor of rcnA
Reads supporting (aligned to +/- strand):  ref base G (149/295);  new base T (53/2);  total (202/297)
Fisher's exact test for biased strand distribution p-value = 9.89e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 507 total aligned reads displayed.

GCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGC  >  NC_000913/2185286‑2185584
                                                                                                                                                     |                                                                                                                                                     
 cagGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGc                                                                                                                                                      <  1:3283502/150‑1 (MQ=255)
 cagGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGc                                                                                                                                                      <  1:769671/150‑1 (MQ=255)
  agGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGACGGGCACCCCGAGTCGTTGGGGTGCg                                                                                                                                                     >  2:7857338/1‑150 (MQ=255)
   ggTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGTCCTGGTGGTCCGTGTCTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCga                                                                                                                                                    <  1:6580250/150‑1 (MQ=255)
   ggTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCga                                                                                                                                                    <  1:4289866/150‑1 (MQ=255)
   ggTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCga                                                                                                                                                    >  1:2723848/1‑150 (MQ=255)
   ggTCGACGTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCga                                                                                                                                                    <  1:6844488/150‑1 (MQ=255)
    gTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCgat                                                                                                                                                   >  2:3078591/1‑150 (MQ=255)
      cGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCgatga                                                                                                                                                 <  2:8325105/150‑1 (MQ=255)
      cGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCgatga                                                                                                                                                 <  1:7006549/150‑1 (MQ=255)
       gACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCgatgat                                                                                                                                                <  1:4818142/150‑1 (MQ=255)
        aCTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATa                                                                                                                                               <  1:3362671/150‑1 (MQ=255)
         cTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATaa                                                                                                                                              >  1:9261967/1‑150 (MQ=255)
          ttGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTTCGATGATaaa                                                                                                                                             >  1:5298997/1‑150 (MQ=255)
             cATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCg                                                                                                                                          >  1:8841075/1‑150 (MQ=255)
                  tttGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGTTAAATAGCagag                                                                                                                                     >  2:3420009/1‑150 (MQ=255)
                  tttGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCagag                                                                                                                                     >  2:4755662/1‑150 (MQ=255)
                    tGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAgagga                                                                                                                                   <  2:4061510/150‑1 (MQ=255)
                    tGCTCCTGCCATAACGAGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCACCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAgagga                                                                                                                                   <  1:7996232/150‑1 (MQ=255)
                       tCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGTTAAATCGCAGAggagga                                                                                                                                >  2:9178072/1‑150 (MQ=255)
                        ccTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGAt                                                                                                                               <  2:6993797/150‑1 (MQ=255)
                         cTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATg                                                                                                                              <  1:6441639/150‑1 (MQ=255)
                          tGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGCCACCCCGAGTCGTTTTTTTGCGATGATAAAACACAGAGCAGGATgg                                                                                                                             >  1:3093538/1‑150 (MQ=255)
                            ccATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTa                                                                                                                           >  2:2344442/1‑150 (MQ=255)
                            ccATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATTATAAATCGCAGAGGAGGATTGTa                                                                                                                           >  1:7380262/1‑150 (MQ=255)
                             cATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAAAAGCAGAGGAGGATGGTaa                                                                                                                          >  2:2813793/1‑150 (MQ=255)
                               tAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGGGGTTGCGGTGTTAAATAGCAGAGGAGGATGGTGATg                                                                                                                        >  1:287271/1‑150 (MQ=255)
                                  cGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCGGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTcc                                                                                                                     <  1:2102374/150‑1 (MQ=255)
                                   gTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGCTGATAAAAAGCAGAGGAGGATGGTAATGTCCa                                                                                                                    >  2:7542394/1‑150 (MQ=255)
                                     gaagaaGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTTGTTGCGATGATAAATCGCAGAGGAGGATTGTAATGTCCAgc                                                                                                                  >  2:6078707/1‑150 (MQ=255)
                                     gaagaaGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTTGTTGCGATGATAAATCGCAGAGGAGGGTGGTAATGTCCAgc                                                                                                                  >  2:6668231/1‑150 (MQ=255)
                                      aagaagCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATCGCAGAGGAGGGTGGGAATGTCCcgcg                                                                                                                 >  2:58407/1‑150 (MQ=255)
                                      aagaagCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAAAAGCAGAGGTGGATGGTAAGGTCCAgcg                                                                                                                 >  1:1392908/1‑150 (MQ=255)
                                           gCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCAcgc                                                                                                            <  1:3497100/150‑1 (MQ=255)
                                               tGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATAGCAGAGGAGGATGGTAATGTCCAGCGCACGCGGTg                                                                                                        >  1:9533127/1‑150 (MQ=255)
                                                 aCCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTa                                                                                                      <  1:2225658/150‑1 (MQ=255)
                                                   cttgtggtCCTTGAATTCCCTACGCTGTCATTATCCAGATCAGGTGATACGGTTATTTCTCAGACTTCACGCAGAAGGGCAACCCGAGTCTTTTGTTTTCGATGATAATTCGCTGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTaaa                                                                                                    <  2:4912829/147‑1 (MQ=255)
                                                   cTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTaaa                                                                                                    <  2:5000009/150‑1 (MQ=255)
                                                   cTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTaaa                                                                                                    <  1:4626878/150‑1 (MQ=255)
                                                    tggtggTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCCGCGCCCGCGTTGTAAAc                                                                                                   >  2:7757674/1‑150 (MQ=255)
                                                     ggtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTTGTTTCGCTGATAAATAGCAGAGGAGGATGGGAATGTACCGAGCACGCGGTGTaaaag                                                                                                  >  1:8229531/1‑148 (MQ=255)
                                                      gtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGa                                                                                                 <  2:4230382/150‑1 (MQ=255)
                                                      gtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGa                                                                                                 <  2:6386473/150‑1 (MQ=255)
                                                      gtggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGa                                                                                                 <  2:8468218/150‑1 (MQ=255)
                                                        ggtCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGGTGCGATGATAAATAGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAgg                                                                                               >  1:1987954/1‑150 (MQ=255)
                                                          tCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGGTGCGATGATAAATAGCCGAGGAGGATGGTAATGTACAGCGCCCGCGGTGTAAACGAGGaa                                                                                             >  1:5116324/1‑150 (MQ=255)
                                                            cGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTGGATGATAAATAGCAGAGGCGGCTTGTAATGTCCAGCGCACGCGTTGTAAACGAGGaaaa                                                                                           >  2:957089/1‑150 (MQ=255)
                                                             gTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGaaaaa                                                                                          <  2:4350501/150‑1 (MQ=255)
                                                                aCTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGGGGTTGCGAGGATAAAAACCAGAGGAGGAGGGGAATGTCCCCCCCACCCCGTGTAAACGAGGAAAAAAgg                                                                                       >  1:9712619/1‑150 (MQ=255)
                                                                  ttCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGGTGATAAAAAGCAGAGGAGGATGGGAATTTGCAGCGCACCCCGTGTAAACCAGGaaaaaacgca                                                                                     >  1:1695449/1‑145 (MQ=255)
                                                                    cccTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTTCGATGATAAATAGCAGAGGAGGATGGTAAAGTCCAGCGCACCCGTTGTAAAAGAGGAAAAACGGCAGt                                                                                   >  2:1806558/1‑150 (MQ=255)
                                                                     ccTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTGGATGTTTAATCGCAGAGGGGGATGGTTATGTGCCGCGCACGCGTGGTAAAAGAGGAAAAAACGCAggt                                                                                  >  1:24/1‑148 (MQ=255)
                                                                       tACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGtttt                                                                                >  2:8704643/1‑150 (MQ=255)
                                                                            tGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGGTTTACaag                                                                           >  2:6490639/1‑149 (MQ=255)
                                                                               cATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTGGGTTGCGATGATAAAACGCAGAGGAGGTTGGTAAGGTCCCGCGCCCGCGGTGTCAAACAGGAAAAAACGCAGGTTTAAAAAcgc                                                                        >  2:2511632/1‑150 (MQ=255)
                                                                               cATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTGGATTTAAAATAGCAGCGGGGGGTGGTGAAGTCCCGCGCACGCGGTGTAAACCAGGAAAAAACGCACTTTTAAAAAcgc                                                                        >  1:3823441/1‑150 (MQ=255)
                                                                                 ttATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTTCGATGATAAAAAGCAGAGGAGGATTGTAAAGTCCAGCGCACCCGTTGTAAAACAGGAAAAAACGCAGTTTTACAAACGCGt                                                                      >  2:3561140/1‑150 (MQ=255)
                                                                                  tATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGATGATAAATAGCAGAGGAGGATGGTAATTTACAGCGCACGCGTTTTAAAAGAGGAAAAACCGCACTTTTAAAAAAGCGtt                                                                     >  1:5402008/1‑150 (MQ=255)
                                                                                   aTCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTGTGGTTTCGATGATAAAAAGCAGCGGGGGCTGGTAAGGTCCCGCGCACCCGGTGTAAACCAGGGAAAACCGCAGGTTTACAAACGCGGta                                                                    >  2:240440/1‑150 (MQ=255)
                                                                                    tCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTTCGATGATAAAAAGCAGAGGAGGATGGTAATGTCCAGCGCACGCGGTGTAAACGATGAAAAAAGGCAGTTTTAAAAACGCGGtaa                                                                   >  2:1304414/1‑150 (MQ=255)
                                                                                     ccAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTTGGATGATAAATAGCAGCGGAGGATGGTTAAGTACAGCCCACGCGTTTTAAAAAAGGAAAAACCGCAGTTTTAAAAAAGCGGtaaa                                                                  >  1:722649/1‑149 (MQ=255)
                                                                                      cAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATTAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTtaata                                                                 <  2:3308889/150‑1 (MQ=255)
                                                                                       aGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTtaataa                                                                <  2:6884574/150‑1 (MQ=255)
                                                                                        gATCTGGTGATACGGGTATTTCTCAGCCTTCACGCAGACGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAACACGGCAGTTTTACAATCGCGTTAATaaa                                                               <  1:1599522/150‑1 (MQ=255)
                                                                                          tCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGATTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGAAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAatt                                                             <  2:4608662/150‑1 (MQ=255)
                                                                                             ggTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCTTTTGGTTGCGATTATAAATCGCAGTGGAGGTTGTTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAattatt                                                          <  1:4448916/150‑1 (MQ=255)
                                                                                             ggTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAattatt                                                          <  2:623071/150‑1 (MQ=255)
                                                                                              gTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTAttt                                                         <  2:7456724/150‑1 (MQ=255)
                                                                                              gTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTAttt                                                         >  2:7140270/1‑150 (MQ=255)
                                                                                                gATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATTATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGa                                                       <  1:7532432/150‑1 (MQ=255)
                                                                                                 aTACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGTTGATAAATCGCAGTGGAGGATGGTATTGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGat                                                      <  1:8373194/150‑1 (MQ=255)
                                                                                                     gggTATTTCTCAGCCTTCACGCAGATGGGCACCCCGAGTCGTTTGGTTGCTATTATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGTAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGatatat                                                  <  1:4008862/150‑1 (MQ=255)
                                                                                                      ggTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATg                                                 <  2:7701248/150‑1 (MQ=255)
                                                                                                            tctcAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCa                                           <  2:1179994/150‑1 (MQ=255)
                                                                                                              tcAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATTATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCTTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGc                                         <  1:8700202/150‑1 (MQ=255)
                                                                                                              tcAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGc                                         <  1:123025/150‑1 (MQ=255)
                                                                                                                       aCGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGa                                <  2:3961978/150‑1 (MQ=255)
                                                                                                                        cGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAGACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGaa                               <  1:5655896/150‑1 (MQ=255)
                                                                                                                        cGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGaa                               <  2:8946142/150‑1 (MQ=255)
                                                                                                                        cGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGaa                               <  2:5725405/150‑1 (MQ=255)
                                                                                                                         gCAGAAGGGCACCCCGTGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAAc                              <  1:6224071/150‑1 (MQ=255)
                                                                                                                         gCAGAAGGGCACCCCGAGTCGTTTGGTTTCGATGATAAATAGCAGAGGAGGATGGTAATTTCCAGCGCAAGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAAACGCGTTAATAAATTATTTTATATATGAATCCAGCACCTTAAGaaa                              >  2:5410780/1‑149 (MQ=255)
                                                                                                                         gCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAAc                              <  2:3233420/150‑1 (MQ=255)
                                                                                                                           aGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCTGAGGAGGTTGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGa                            <  1:8129922/150‑1 (MQ=255)
                                                                                                                             aaGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACa                          <  1:9391838/150‑1 (MQ=255)
                                                                                                                               gggCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATc                        <  2:3756399/150‑1 (MQ=255)
                                                                                                                                       cGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGAtctt                <  1:2562102/150‑1 (MQ=255)
                                                                                                                                       cGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGAtctt                <  1:5316103/150‑1 (MQ=255)
                                                                                                                                         aGTCGTTTGGTTGCGATGTTAAATAGCAGAGGAGGATGGTAATGTCCAGCGCACGAGTTTTAAACGAGGAAAAAAGGCAGTTTTACAATCGCGTTAATTAATTATTTGTTATATGAATCCAGCACCTTCATAACGTCATCCAGAtcttct              <  2:4301255/150‑1 (MQ=255)
                                                                                                                                         aGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGAtcttct              <  2:9325339/150‑1 (MQ=255)
                                                                                                                                            cGTTTGGTTGCGATTATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCa           <  1:1806558/150‑1 (MQ=255)
                                                                                                                                             gTTTGGTTGCGATGATATATCGCAGAGGTGGAGGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCAc          <  2:88898/150‑1 (MQ=255)
                                                                                                                                               ttGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGt        <  1:5973518/150‑1 (MQ=255)
                                                                                                                                               ttGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGt        <  2:5182418/150‑1 (MQ=255)
                                                                                                                                                  gTTGCGATCATACATCGCAGAGGAGGATGGTAATGACCAGCGCACTCGTTGTAAACGAGGAAAAACGCCAGTTTTACAATCCCGTTAATAAATTATTTGATATATGAATCCAGCAACTTCAGAACGACATCCAGAACTTCTTAAAGtttt     <  2:2051600/150‑1 (MQ=255)
                                                                                                                                                   ttGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAGCGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTa    <  2:8035199/150‑1 (MQ=255)
                                                                                                                                                   ttGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTa    <  2:2180207/150‑1 (MQ=255)
                                                                                                                                                     gCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGc  <  1:8525223/150‑1 (MQ=255)
                                                                                                                                                     gCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGc  <  2:1555086/150‑1 (MQ=255)
                                                                                                                                                     gCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGc  <  1:6947054/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAGGTCGACTTGCATAGTTTGCTCCTGCCATAACGTGAAGAAGCAATGACCTGGTGGTCCGTGACTTCCCTACGCTGGCATTATCCAGATCAGGTGATACGGGTATTTCTCAGCCTTCACGCAGAAGGGCACCCCGAGTCGTTTGGTTGCGATGATAAATCGCAGAGGAGGATGGTAATGTCCAGCGCACGCGTTGTAAACGAGGAAAAACGGCAGTTTTACAATCGCGTTAATAAATTATTTGATATATGAATCCAGCACCTTCAGAACGACATCCAGATCTTCTTCACGTTTTAGC  >  NC_000913/2185286‑2185584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: