Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,441 C→A 5.9% D437E (GAC→GAA gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4410CA 5.9% 1271.1 / 8.2 388D437E (GAC→GAAgtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base C (143/205);  new base A (35/5);  total (178/210)
Fisher's exact test for biased strand distribution p-value = 1.31e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 393 total aligned reads displayed.

TTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTT  >  NC_000913/2470292‑2470590
                                                                                                                                                     |                                                                                                                                                     
tttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGTATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCTTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTTTAAAATAATTCAAATAAATAAAGAc                                                                                                                                                       >  1:6117041/1‑150 (MQ=255)
tttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAATAAAAAc                                                                                                                                                       >  2:8394227/1‑150 (MQ=255)
 ttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACa                                                                                                                                                      <  1:7939982/150‑1 (MQ=255)
    gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATAAAGACAATa                                                                                                                                                   >  1:7257060/1‑150 (MQ=255)
        atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTTTTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATTACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                               <  2:6854523/150‑1 (MQ=255)
        atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                               <  2:6425462/150‑1 (MQ=255)
        atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                               <  1:3962813/150‑1 (MQ=255)
         tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAAAATCGag                                                                                                                                              >  2:1741516/1‑149 (MQ=255)
            cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAATAAATAAAGAAAAAAGCGATGTa                                                                                                                                           >  2:6229550/1‑150 (MQ=255)
            cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAATCAAGAAAAAATCGATGTa                                                                                                                                           >  1:2989953/1‑150 (MQ=255)
               ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAAACAAAAAAATATCGAATTaaaa                                                                                                                                        >  2:1977722/1‑149 (MQ=255)
                     gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                                  <  1:4028719/150‑1 (MQ=255)
                       gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGt                                                                                                                                <  2:9684286/150‑1 (MQ=255)
                       gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGATGTAAAGCACTAAGt                                                                                                                                >  2:4889005/1‑150 (MQ=255)
                        tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                               <  1:4666464/150‑1 (MQ=255)
                        tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAAAATCGATTTAAAGAACTAAGtt                                                                                                                               >  2:8118544/1‑150 (MQ=255)
                        tCATTTTTAGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAAAAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATAAAGACCATAGCGATGTAAAGCACTAAGtt                                                                                                                               >  2:5352575/1‑150 (MQ=255)
                         cATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGttt                                                                                                                              <  2:6274469/150‑1 (MQ=255)
                          aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAAAAGCGATGTAAAGCACTAAGTTTa                                                                                                                             >  2:3924009/1‑150 (MQ=255)
                            ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                           <  2:1453276/150‑1 (MQ=255)
                                  gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAATCAAGAAAAAAGCGATGTAAATAACTAAGTTTAAATTtcgc                                                                                                                     >  2:3344743/1‑150 (MQ=255)
                                   ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAAGAAAAAAATACAAAAGCGATGTAAAGCACTAAGTTTAAATTtcgag                                                                                                                    >  1:5276216/1‑148 (MQ=255)
                                    gAAGGATATCGAGATTTAAGCAAGCATGTAGTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAACTTgcgcgc                                                                                                                   <  2:3071019/150‑1 (MQ=255)
                                    gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcgc                                                                                                                   <  2:954752/150‑1 (MQ=255)
                                        gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAAACAATAGAGATGTAAAGAAAGAAGTTTAAATTGCGCGCCaag                                                                                                               >  1:5030248/1‑149 (MQ=255)
                                           ataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAt                                                                                                            <  1:3256849/150‑1 (MQ=255)
                                           ataGAGAATTAATCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTATTTTATAATATAATTCAAAGAAATCAAGACAATAGCGTTGTATAGCTCTAAGTTTAAATTGCGCGCCAATCAt                                                                                                            <  2:9388354/150‑1 (MQ=255)
                                            taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                           <  1:7472031/150‑1 (MQ=255)
                                            taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                           <  2:8861388/150‑1 (MQ=255)
                                              gagctgtAAGCAAGCATTGATTGGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                                         <  1:5872358/144‑1 (MQ=255)
                                              gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                                         <  2:7712511/150‑1 (MQ=255)
                                              gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                                         <  2:6634805/150‑1 (MQ=255)
                                                gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                                       <  1:7707379/150‑1 (MQ=255)
                                                 aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAAAAAACAATAAAAAAACGAATTAAAGCACTAATTTTAAATTGAGCGCCAAACAAGgcgcg                                                                                                      >  2:75294/1‑150 (MQ=255)
                                                 aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAAATTATAAAATAAAAAAAATAAATAAAAAAAAAAACGATGTAAAGCACTAAGGTTAAAATTCGCGCCCATCagttcgcg                                                                                                      >  1:2088855/1‑143 (MQ=255)
                                                    tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATTAAATAATTAAAATAAATAAATAAAATAGCGAAGTGAATCACAAAGTTTAAATTTAGCGCCCCTCATGGCGCGcac                                                                                                   >  1:6469981/1‑150 (MQ=255)
                                                       gcaagcaTTTATTTTGAATGTATTTTTAGTTCGACCTTTGCTTATACATTACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAg                                                                                                <  2:5650058/150‑1 (MQ=255)
                                                       gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAg                                                                                                <  2:1842682/150‑1 (MQ=255)
                                                          agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCta                                                                                             <  2:4435038/150‑1 (MQ=255)
                                                           gcaTTTATTTGTATTGTATTTTTCGTTCGACTTTTGATTATACATAACCTTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGAAAATAGCTATGTAAAGCACTATGTTTAATTTGCGCGCCAATCTTGGCGCGCACAAGCtat                                                                                            <  1:5187122/150‑1 (MQ=255)
                                                                   ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCaa                                                                                    >  2:2464504/1‑150 (MQ=255)
                                                                         tGTATTTTTGTTTCGACCTTTGCTTATACTTAACTTTGGTTTTTTTAATTTATAAAATATTTCAAAGAAATCAAGACTATAGCGATGTAAAGCACTATGTTTAATTTGCGGGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAt                                                                              <  1:6860091/150‑1 (MQ=255)
                                                                            aTTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAAACAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCGCACGCTATAATACCCACCAAAtgtg                                                                           >  2:9446343/1‑147 (MQ=255)
                                                                              ttttCGTTCGACCTTTGCTTATACATAACTTTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCTATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                                         <  1:8745108/150‑1 (MQ=255)
                                                                              ttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAGAAATCAAGACAATAGCGATGTAAATCACTAAGTTTAAATTTCGCGCCAAAAAAGGCGCGCACAAGCTATAATACCAACCAAATTtctc                                                                         >  2:271901/1‑150 (MQ=255)
                                                                               tttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcc                                                                        <  2:2332719/150‑1 (MQ=255)
                                                                               tttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcc                                                                        <  2:8030447/150‑1 (MQ=255)
                                                                                   gttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCCAACATGGCGCGCCCAAGCTATAATACCAACCTAAATTctcctcct                                                                    >  2:8590004/1‑150 (MQ=255)
                                                                                      cgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCtt                                                                 <  1:2512557/150‑1 (MQ=255)
                                                                                       gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAGAAATCAATAAAAATGCGAAGTAAAGCACTAAGTTTAAATTTAGCGACAAAAATGGCGCGCACAAACTATAAAAACAACCTAATATatcctcctaata                                                                >  2:216522/1‑146 (MQ=255)
                                                                                            ttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTg                                                           <  1:6234033/150‑1 (MQ=255)
                                                                                            ttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAATAGCGATGTAAAGCACTAAGTTTACATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTg                                                           <  2:7603415/150‑1 (MQ=255)
                                                                                              gCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                                         <  1:4442034/150‑1 (MQ=255)
                                                                                              gCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                                         <  1:5694023/150‑1 (MQ=255)
                                                                                              gCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                                         <  2:4144906/150‑1 (MQ=255)
                                                                                                 tataCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACtat                                                      <  1:2418484/150‑1 (MQ=255)
                                                                                                   taCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAATTCTCCTCCTCTTAGAGTGACTatat                                                    >  2:7942224/1‑150 (MQ=255)
                                                                                                          cAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                                             <  1:1403378/150‑1 (MQ=255)
                                                                                                             tCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCATGATAg                                          >  2:1133517/1‑150 (MQ=255)
                                                                                                                tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                       >  2:6989790/1‑150 (MQ=255)
                                                                                                                   ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                                    <  1:743222/150‑1 (MQ=255)
                                                                                                                   ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                                    <  1:9656375/150‑1 (MQ=255)
                                                                                                                    tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                   <  1:2315323/150‑1 (MQ=255)
                                                                                                                    tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                   <  2:9120354/150‑1 (MQ=255)
                                                                                                                    tttAATTTATAAAATAATACAAAGAAATCAAGACAATAGCGATGTAAACCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                   <  1:4874124/150‑1 (MQ=255)
                                                                                                                     ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                                  <  1:3642167/150‑1 (MQ=255)
                                                                                                                      tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTCTAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                                 >  1:9247417/1‑150 (MQ=255)
                                                                                                                        aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                               <  2:8331015/150‑1 (MQ=255)
                                                                                                                         tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                              <  2:2777959/150‑1 (MQ=255)
                                                                                                                         tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAtt                              <  1:1617567/150‑1 (MQ=255)
                                                                                                                            ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                           <  2:7767501/150‑1 (MQ=255)
                                                                                                                               aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt                        <  1:7992822/150‑1 (MQ=255)
                                                                                                                               aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt                        <  1:4125739/150‑1 (MQ=255)
                                                                                                                                aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc                       >  2:5829193/1‑150 (MQ=255)
                                                                                                                                   aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa                    <  2:2916314/150‑1 (MQ=255)
                                                                                                                                     ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg                  <  2:7383212/150‑1 (MQ=255)
                                                                                                                                     ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg                  <  2:8244463/150‑1 (MQ=255)
                                                                                                                                     ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg                  >  1:8482850/1‑150 (MQ=255)
                                                                                                                                      tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc                 >  2:5535521/1‑150 (MQ=255)
                                                                                                                                      tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc                 <  1:1567614/150‑1 (MQ=255)
                                                                                                                                      tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc                 <  1:8076260/150‑1 (MQ=255)
                                                                                                                                      tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc                 >  2:1032462/1‑150 (MQ=255)
                                                                                                                                       cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc                <  2:3719348/150‑1 (MQ=255)
                                                                                                                                         aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt              >  1:2882673/1‑150 (MQ=255)
                                                                                                                                         aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt              >  1:8330182/1‑150 (MQ=255)
                                                                                                                                         aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt              <  2:7878201/150‑1 (MQ=255)
                                                                                                                                          aGAAATCAAGACCATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTg             <  2:5177842/150‑1 (MQ=255)
                                                                                                                                          aGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTg             >  1:3701318/1‑150 (MQ=255)
                                                                                                                                              aTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTc         <  2:2536935/150‑1 (MQ=255)
                                                                                                                                                cAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgt       <  2:5256196/150‑1 (MQ=255)
                                                                                                                                                cAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgt       <  1:3476047/150‑1 (MQ=255)
                                                                                                                                                 aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt      >  2:589990/1‑150 (MQ=255)
                                                                                                                                                 aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt      >  1:1845519/1‑150 (MQ=255)
                                                                                                                                                 aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt      >  1:8835652/1‑150 (MQ=255)
                                                                                                                                                 aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt      >  1:1072866/1‑150 (MQ=255)
                                                                                                                                                  aGACAATAGCGATGTAAAGCACTAAGTTTAATTTGCGCGGGTATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTTGAGTGACTATATCTCCTGATAGTATTTCGGTATTGACTATCTAATGCCCTGATTCgttg     >  1:6627263/1‑150 (MQ=255)
                                                                                                                                                  aGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgttg     <  2:7139440/150‑1 (MQ=255)
                                                                                                                                                    aCAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgttgtt   >  1:1681824/1‑150 (MQ=255)
                                                                                                                                                    aCAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgttgtt   >  1:6977844/1‑150 (MQ=255)
                                                                                                                                                    aCAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgttgtt   <  1:4787154/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTT  >  NC_000913/2470292‑2470590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: