Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,725,850 T→A 6.9% intergenic (‑104/+219) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,725,8500TA 6.9% 1755.4 / 18.5 539intergenic (‑104/+219)kgtP/rrfGalpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base T (173/310);  new base A (48/3);  total (226/313)
Fisher's exact test for biased strand distribution p-value = 8.28e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 541 total aligned reads displayed.

CTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGT  >  NC_000913/2725703‑2725999
                                                                                                                                                   |                                                                                                                                                     
 tactTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAAGGAAATATTATTCATTTGTTGCATATAATTTTTCAACCTTaa                                                                                                                                                    >  1:7668854/1‑150 (MQ=255)
  actTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAc                                                                                                                                                   <  2:7820273/150‑1 (MQ=255)
    ttGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAAATTGTTGAATATCATTATTAAACATTAACCa                                                                                                                                                 >  2:2966600/1‑150 (MQ=255)
       tCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTTTTTTTTTTTTTAAGGAAATATTATAAATTTTTTTCATATAAATATTCAACCCTAAACAAGa                                                                                                                                              >  1:2851116/1‑150 (MQ=255)
            ttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATtta                                                                                                                                         <  1:1557775/150‑1 (MQ=255)
            ttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATtta                                                                                                                                         <  1:823714/150‑1 (MQ=255)
            ttGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAACATGAATtta                                                                                                                                         >  2:3883301/1‑150 (MQ=255)
             cgccgccTGCCGTTCCTGTACTCTCACCCATGCCATTCTGTCTCCTGCCGTAATCCGATGCTCTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttag                                                                                                                                        <  1:5113257/144‑1 (MQ=255)
               cTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGGAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttagtt                                                                                                                                      >  2:8708919/1‑150 (MQ=255)
               cTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTTTTTTTTTTTTTTTTAAAGGTAATAATATACAATTTTTGGATATCATTATTCAACCATAACCAAGAATttagtt                                                                                                                                      >  1:5938899/1‑150 (MQ=255)
                tGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATttagtta                                                                                                                                     <  1:4522015/150‑1 (MQ=255)
                   cTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTGTTTAATATGATTATGCAAACATAAACATTAATTTTGTTTGCa                                                                                                                                  >  2:9056403/1‑150 (MQ=255)
                    tGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTTTAAAGGAAATATTATAAATTTTTTGCATAAAATTATGCAACCATAAACAAGAATGTAGTTAGCAg                                                                                                                                 >  2:1752133/1‑150 (MQ=255)
                    tGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTTTTTTTTTGTTAAGGCAATATTAAAAAATTGTTTCATATCATTATTCAACCAAAACCATGAATTTAGTTAGCAgg                                                                                                                                >  1:313185/1‑150 (MQ=255)
                      ccGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTTAAGGAAATATTATACATTTTTTTCATATAATTATGCAACCTTAACCATGAATTTAGTTAGCAGGa                                                                                                                               >  2:7511482/1‑150 (MQ=255)
                      ccGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTTTATGGAATAATATAAAAATGTTTCTTATAATTATGCAAACATAAACAAGAATTGTGTTAGCAGGa                                                                                                                               >  1:530500/1‑150 (MQ=255)
                      ccGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTGTGGTTATTTTTTTTTTAAATGCAAAATTATAAATTTGTTTGATATAATTATTCAACCATAAACATGAATTTATTTAGCAGGa                                                                                                                               >  1:3945695/1‑150 (MQ=255)
                         ttACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGaaaa                                                                                                                            <  2:110090/150‑1 (MQ=255)
                           aCAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATg                                                                                                                          <  2:8547049/150‑1 (MQ=255)
                            cAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATgg                                                                                                                         <  2:4137520/150‑1 (MQ=255)
                              gTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTGTTTTTTTTTTTTTTTTTTAAGGGAATATTATAAAATTTTTTGATATCATTATGCAAACAAAAACATTAATTTATTTATCAGGAAAATGGtt                                                                                                                       >  1:6629589/1‑150 (MQ=255)
                                aCTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTAt                                                                                                                     <  2:5651771/150‑1 (MQ=255)
                                 cTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTAtt                                                                                                                    <  2:7328608/150‑1 (MQ=255)
                                  tttCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTg                                                                                                                   <  2:5921804/150‑1 (MQ=255)
                                     cAGCCATGCCATTATGTCTCCTGCCGTAATCCGCTGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTgagg                                                                                                                <  1:2670005/150‑1 (MQ=255)
                                            gccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTaa                                                                                                         <  1:4763254/150‑1 (MQ=255)
                                            gccatTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTaa                                                                                                         <  1:7367462/150‑1 (MQ=255)
                                              catTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAAACATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAgg                                                                                                       >  2:2326197/1‑150 (MQ=255)
                                                 tATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATa                                                                                                    <  2:1870475/150‑1 (MQ=255)
                                                 tATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATa                                                                                                    <  1:4191150/150‑1 (MQ=255)
                                                  aTGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATaa                                                                                                   <  1:5837231/150‑1 (MQ=255)
                                                     tctcCTGCCGTAATCCGATGCTTTTGTCGGTCGCGTTTGTTTATTTTTTTTGTGAAGGTAATATTATACATTTGTTGCCTATCAATATTCAACCAAAACCAGTAATTTAGTTTGCAGGAAAATGGGTATTGAGGCGCTTTAGGGTAAAtt                                                                                                >  2:4195711/1‑150 (MQ=255)
                                                       tcCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATAAATTTGTTGCATATAATTATTCAACCTTAAAAATGAATTTATTTATCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAAtttg                                                                                              >  2:6855359/1‑149 (MQ=255)
                                                        ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCTTGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                                             <  2:2686835/150‑1 (MQ=255)
                                                        ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                                             <  2:2115437/150‑1 (MQ=255)
                                                        ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                                             <  2:1019302/150‑1 (MQ=255)
                                                        ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                                             <  2:5519094/150‑1 (MQ=255)
                                                        ccTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCt                                                                                             <  1:7039040/150‑1 (MQ=255)
                                                            ccGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTAATTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTAAGCAACCATAACCATGAATTTAGTTAGCAGGAAAAAGGGTGTTGAGGAGCTTAAGGATAAATTTCTGGtc                                                                                         >  2:3718127/1‑149 (MQ=255)
                                                              gTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAag                                                                                       <  1:6054487/150‑1 (MQ=255)
                                                                    cGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAAAATTATACATTTTTTTAAAATAAATATGCAACCTTAAACAAGAATTTATTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTATTGTAAGGAGGac                                                                                 >  2:3652416/1‑150 (MQ=255)
                                                                     gATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGaca                                                                                <  1:2151995/150‑1 (MQ=255)
                                                                        gCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTAAGCAACCTTAAACATGAAATTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAATGATAAATTTCTGGTAAGGAGGACACGt                                                                             >  2:7213582/1‑150 (MQ=255)
                                                                          ttttGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTAt                                                                           <  1:3725137/150‑1 (MQ=255)
                                                                          ttttGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTAt                                                                           <  2:4821464/150‑1 (MQ=255)
                                                                          ttttGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTAt                                                                           <  1:3788053/150‑1 (MQ=255)
                                                                           tttGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATAATTATGCAAACTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAAGACACGTATg                                                                          >  2:6278707/1‑150 (MQ=255)
                                                                              gtcggtcgCTTTTGTTTATTTTTTTTTTAAAGGAAATAATATAAAATTTTTTCATATCATTATGCAACCATAAACAAGAATTTATTTATCAGGAAAATTGTTATTTAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACAACTATggga                                                                       >  2:6714845/1‑148 (MQ=255)
                                                                              gtcggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGaa                                                                       <  1:927827/150‑1 (MQ=255)
                                                                              gtcggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGaa                                                                       <  2:313130/150‑1 (MQ=255)
                                                                               tcggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAAAATAATAAAATTTTTTCAAATCATTATTCAACCATAAACAAGAATTTAGTTAGCAGGAAAAAGGTTATTGAGGAGCTTAAGGATAAATTTCTTGTAAGGAGGACACGGATGGAAg                                                                      >  2:8379155/1‑150 (MQ=255)
                                                                                cggtcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGt                                                                     <  1:3585858/150‑1 (MQ=255)
                                                                                   tcgCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGTGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTggg                                                                  <  2:8242477/150‑1 (MQ=255)
                                                                                      cTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCaa                                                               >  2:6160373/1‑150 (MQ=255)
                                                                                       ttttttttATATTTTTTGTAAAGGAAATAATATACATTTGTTTCATATCATTATGCAACCTAAACCATGAATTAAGATAGAAGGAAAATGGTTATTTAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAg                                                              >  2:3364303/1‑150 (MQ=255)
                                                                                       ttttGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAg                                                              >  2:8427211/1‑150 (MQ=255)
                                                                                           gTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTgg                                                          <  1:3415104/150‑1 (MQ=255)
                                                                                            tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTgggg                                                         >  2:4607051/1‑150 (MQ=255)
                                                                                            tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                                         <  2:9456999/150‑1 (MQ=255)
                                                                                            tttATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTggg                                                         <  1:8704456/150‑1 (MQ=255)
                                                                                               aTTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGaa                                                      >  1:6961852/1‑150 (MQ=255)
                                                                                                ttttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAg                                                     >  1:459888/1‑150 (MQ=255)
                                                                                                ttttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAg                                                     >  2:9271880/1‑150 (MQ=255)
                                                                                                ttttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTGGGGGAAg                                                     >  2:6380439/1‑150 (MQ=255)
                                                                                                 tttttttGTAATGTATATATTATACTTTTGTTGCATATCTTTATGCAACCTTAACCATGAATTTATTTAGCTGCAAAATGGTTTTTGAGGAGCTTAAGTATAAATTTCTGTTATGGTGGACACGTATGGATGTGGGCAAGTTGGGTAAGc                                                    <  2:1466367/150‑1 (MQ=255)
                                                                                                 tttttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGc                                                    <  2:1233847/150‑1 (MQ=255)
                                                                                                   tttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCg                                                  >  2:5990742/1‑150 (MQ=255)
                                                                                                    ttttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGt                                                 >  2:7046703/1‑150 (MQ=255)
                                                                                                      ttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTAt                                               <  1:2580560/150‑1 (MQ=255)
                                                                                                      ttGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTAt                                               >  1:6174643/1‑150 (MQ=255)
                                                                                                        gTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATcc                                             <  1:1716237/150‑1 (MQ=255)
                                                                                                        gTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATcc                                             >  2:4437678/1‑150 (MQ=255)
                                                                                                         tAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCg                                            >  1:8384637/1‑150 (MQ=255)
                                                                                                         tAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCg                                            >  2:7882521/1‑150 (MQ=255)
                                                                                                         tAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTGGGGGAAGCCGTATCCg                                            >  2:5031960/1‑150 (MQ=255)
                                                                                                           aaGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGTGCTTAAGGATATATTTGTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGtt                                          >  1:1985176/1‑150 (MQ=255)
                                                                                                            aGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTg                                         <  1:111563/150‑1 (MQ=255)
                                                                                                             ggAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGc                                        >  1:7098091/1‑150 (MQ=255)
                                                                                                             ggAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGc                                        >  1:2903352/1‑150 (MQ=255)
                                                                                                                aaTATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGa                                     <  2:2519745/150‑1 (MQ=255)
                                                                                                                      ataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTgg                               >  1:6365129/1‑150 (MQ=255)
                                                                                                                      ataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTgg                               <  1:7317890/150‑1 (MQ=255)
                                                                                                                      ataCATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTgg                               >  1:5863274/1‑150 (MQ=255)
                                                                                                                           tttGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCatat                          <  1:9158332/150‑1 (MQ=255)
                                                                                                                           tttGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCatat                          <  1:7905809/150‑1 (MQ=255)
                                                                                                                           tttGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCatat                          >  2:3261287/1‑150 (MQ=255)
                                                                                                                               ttgCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTgg                      >  1:5518205/1‑150 (MQ=255)
                                                                                                                                  cATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAg                   >  2:5224541/1‑150 (MQ=255)
                                                                                                                                  cATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAg                   >  2:4889202/1‑150 (MQ=255)
                                                                                                                                     atCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGtat                <  2:28409/150‑1 (MQ=255)
                                                                                                                                      tCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGtata               >  2:2432322/1‑150 (MQ=255)
                                                                                                                                       cATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATaa              <  2:7705221/150‑1 (MQ=255)
                                                                                                                                       cATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATaa              <  2:3977357/150‑1 (MQ=255)
                                                                                                                                        aTTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTCAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAg             >  1:9543770/1‑150 (MQ=255)
                                                                                                                                        aTTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAg             >  2:8872575/1‑150 (MQ=255)
                                                                                                                                         ttATGCATCCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGa            <  2:6981560/150‑1 (MQ=255)
                                                                                                                                         ttATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGa            <  1:2405557/150‑1 (MQ=255)
                                                                                                                                              cAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACgcgc       <  2:431845/150‑1 (MQ=255)
                                                                                                                                                  cTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTATGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCTAGTTGGGGAAGCCGTATCCGTTGCTGTATCTGGCTTATGTGGGAGTATAAGTCGCGCAGCg   >  1:4370828/1‑150 (MQ=255)
                                                                                                                                                  cTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCg   >  1:895391/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CTACTTGTCAGTTTGCTGTCTGCCGTTACAGTACTTTCAGCCATGCCATTATGTCTCCTGCCGTAATCCGATGCTTTTGTCGGTCGCTTTTGTTTATTTTTTTTGTAAAGGAAATATTATACATTTGTTGCATATCATTATGCAACCTTAACCATGAATTTAGTTAGCAGGAAAATGGTTATTGAGGAGCTTAAGGATAAATTTCTGGTAAGGAGGACACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGT  >  NC_000913/2725703‑2725999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: