Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,947,470 A→T 5.6% intergenic (+7/‑27) metZ → / → metW tRNA‑Met/tRNA‑Met

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,947,4700AT 5.6% 1083.3 / 2.7 377intergenic (+7/‑27)metZ/metWtRNA‑Met/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (104/225);  new base T (8/23);  total (116/261)
Fisher's exact test for biased strand distribution p-value = 5.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 382 total aligned reads displayed.

ATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTG  >  NC_000913/2947321‑2947615
                                                                                                                                                     |                                                                                                                                                  
aTCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTaaaa                                                                                                                                                    <  2:2937348/150‑1 (MQ=255)
 tCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAt                                                                                                                                                   <  1:6993497/150‑1 (MQ=255)
   cGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGATGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAttt                                                                                                                                                 <  2:8299512/150‑1 (MQ=255)
   cGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAAttt                                                                                                                                                 <  2:9495423/150‑1 (MQ=255)
    gTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTtg                                                                                                                                                >  2:6237600/1‑150 (MQ=255)
     tGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtga                                                                                                                                               <  1:1337517/150‑1 (MQ=255)
     tGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtga                                                                                                                                               <  1:7665224/150‑1 (MQ=255)
     tGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtga                                                                                                                                               <  1:2258550/150‑1 (MQ=255)
      gATCGGGTTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgat                                                                                                                                              <  2:8982878/150‑1 (MQ=255)
       aTCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTtgatg                                                                                                                                             <  1:8638482/150‑1 (MQ=255)
         cGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGaa                                                                                                                                           <  1:5298126/150‑1 (MQ=255)
          ggggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTTATGAAg                                                                                                                                          >  1:5549808/1‑150 (MQ=255)
           gggTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGt                                                                                                                                         >  2:2634347/1‑150 (MQ=255)
               aaaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAg                                                                                                                                    <  2:3407670/150‑1 (MQ=255)
                aaaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGCTCGTCGGTTCAAAACCGGCCCCCGAAACCAAATAAAATTTGATCAAGT‑aaatc                                                                                                                                   >  1:4009306/1‑148 (MQ=21)
                 aaaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCa                                                                                                                                  <  1:4966525/150‑1 (MQ=255)
                  aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTTATGACTT‑AAAGCAg                                                                                                                                 >  2:7871259/1‑150 (MQ=255)
                  aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGT‑aaaagca                                                                                                                                 >  1:4720039/1‑145 (MQ=255)
                  aaaaaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGT‑aaaagca                                                                                                                                 <  2:4720039/149‑5 (MQ=255)
                     aaGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTAc                                                                                                                              >  2:1689301/1‑150 (MQ=255)
                       ggTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATAGAGTGAAGA‑AAAGCactcctg                                                                                                                            >  1:7474597/1‑144 (MQ=21)
                         ttGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAATTTGATAAAGT‑ATAGCACTACCGTg                                                                                                                          >  2:4140205/1‑150 (MQ=21)
                         ttGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAA‑TCAAATTTGATGAAGTAAAAGCAGTACGGTg                                                                                                                          <  2:6400364/150‑1 (MQ=255)
                                  aaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGt                                                                                                                 <  2:6702460/150‑1 (MQ=255)
                                  aaCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGt                                                                                                                 <  1:8761618/150‑1 (MQ=255)
                                     gcgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGa                                                                                                              <  2:2106493/150‑1 (MQ=255)
                                     gcgAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGa                                                                                                              <  2:8420274/150‑1 (MQ=255)
                                          cGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGagcagc                                                                                                         <  1:5813727/150‑1 (MQ=255)
                                           ggAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGcc                                                                                                        <  2:8490618/150‑1 (MQ=255)
                                            gAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCt                                                                                                       <  2:1112245/150‑1 (MQ=255)
                                             aGTATAGTGCGCATCCACGGACGCTGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTg                                                                                                      <  2:5293788/150‑1 (MQ=255)
                                               tataGTTCGCATCCACGGACGCGGTGTGGATCAGCCTGGTATCTCGTCGGGATCATAACCCGAAGGTCGTCGGTTCAAAACCGGCCACCGCAACCAAATAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGt                                                                                                    <  2:5668978/150‑1 (MQ=17)
                                                ataGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTa                                                                                                   <  1:7274216/150‑1 (MQ=255)
                                                 taGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAg                                                                                                  <  1:2114440/150‑1 (MQ=255)
                                                     gcgcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGGTCAAATCCGGCCCCCGCACCCAATTAAAATTTGATGACGG‑AAAGCAGGACGGTGACGCGGGGTGGCGCAGCCTGGTcgcgcg                                                                                              >  1:8155349/1‑150 (MQ=17)
                                                       gcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAGTAAGAGGAAGG‑TAGGCATTACGGTTAAGAGGTGTGGAGAAGACTGGGAGCtcgac                                                                                            >  2:3158500/1‑148 (MQ=14)
                                                       gcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtc                                                                                            <  1:1470902/150‑1 (MQ=255)
                                                       gcATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCtcgtc                                                                                            <  1:4144419/150‑1 (MQ=255)
                                                          tCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCggg                                                                                         <  2:3626405/150‑1 (MQ=255)
                                                              cGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATATCCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTTCGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCa                                                                                     <  2:7793574/150‑1 (MQ=255)
                                                              cGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCa                                                                                     <  2:4918690/150‑1 (MQ=255)
                                                                 aCGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATaa                                                                                  <  2:4447683/150‑1 (MQ=40)
                                                                   gcgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAcc                                                                                <  1:5644347/150‑1 (MQ=40)
                                                                   gcgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAcc                                                                                <  1:8292155/150‑1 (MQ=40)
                                                                    cgGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAccc                                                                               <  1:3655141/150‑1 (MQ=40)
                                                                     ggggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCg                                                                              <  1:2634347/150‑1 (MQ=40)
                                                                     ggggTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCg                                                                              <  1:3162513/150‑1 (MQ=40)
                                                                           gAGCAGCCTGTTAGCTCGTCGGGCTCATACCCCGAAGGTCGTCGGTTCATATCCGGCCCCCGCAACCAATTATCTTTTGCTGAAGT‑AAACGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtc                                                                        <  2:1921212/150‑1 (MQ=11)
                                                                           gAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtc                                                                        >  1:6215806/1‑150 (MQ=40)
                                                                           gAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGGAGCTCGTCGGGCGCATAACCCGAAgggc                                                                        >  1:1171179/1‑148 (MQ=255)
                                                                              cagcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGAGGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGACGgtcgtc                                                                     >  1:8446448/1‑150 (MQ=255)
                                                                                gcCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCTCAACCAATTCTATTTTGATGAAGT‑TAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCgg                                                                   <  2:5034607/150‑1 (MQ=11)
                                                                                 ccTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCTAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGt                                                                  <  1:6766817/150‑1 (MQ=255)
                                                                                  cTGGTTTCTCGTCGGTCTCATAACCCTAAGGTGGTCGGTTGATATCCGGACCCCGCAACCAATTATATTTTGATGAAGT‑AAAGGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGtt                                                                 <  2:1486467/150‑1 (MQ=11)
                                                                                  cTGGTCGCTCGTCTGGCTCATAACCCGAAGGTGGTCGGTTCCAATCCGGCCCCCGCAACCAATTATACTTTGTTGAAGT‑AACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGtt                                                                 <  2:4100623/150‑1 (MQ=11)
                                                                                  cTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGTCCCCCGCAACCAATTATACTTTGATGAAGT‑CACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGtt                                                                 <  1:9306866/150‑1 (MQ=11)
                                                                                  cTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑ATAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGtt                                                                 <  2:8195304/150‑1 (MQ=255)
                                                                                  cTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGtt                                                                 <  1:6834860/150‑1 (MQ=40)
                                                                                   tGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTc                                                                >  1:5745706/1‑150 (MQ=40)
                                                                                      tAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCaaa                                                             <  1:1689301/150‑1 (MQ=40)
                                                                                      tAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCaaa                                                             <  2:6135140/150‑1 (MQ=40)
                                                                                       aGCTCGTCGGGCTCATAACCCTAAGGTCGTCGGTTCAAATCCGTCCCCCGCAACCAATTAACATTTGATGAAGT‑AACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAAt                                                            <  2:9170636/150‑1 (MQ=255)
                                                                                        gCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATc                                                           <  2:3668590/150‑1 (MQ=40)
                                                                                         cTCGTCGGGCTCATAAGCCGAATGTCGTCGGTGCATATCCGGCCCCCGCAACCAATTACATTTTGATGAAGT‑CATGGAGTACTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATcc                                                          <  1:7920193/150‑1 (MQ=11)
                                                                                         cTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATcc                                                          <  1:639572/150‑1 (MQ=40)
                                                                                           cgtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCgg                                                        <  1:9688865/150‑1 (MQ=40)
                                                                                            gtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAATTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGc                                                       >  1:9528058/1‑150 (MQ=38)
                                                                                            gtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGc                                                       <  2:8761849/150‑1 (MQ=40)
                                                                                            gtcgGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTAAGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGc                                                       >  1:9026818/1‑150 (MQ=255)
                                                                                                    cATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCaa                                               <  2:7769508/150‑1 (MQ=40)
                                                                                                     aTAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc                                              <  1:4292066/150‑1 (MQ=40)
                                                                                                         cccGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt                                          <  2:7408289/150‑1 (MQ=40)
                                                                                                           cGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCa                                        >  1:6026076/1‑150 (MQ=255)
                                                                                                            gAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaa                                       >  1:3276970/1‑150 (MQ=255)
                                                                                                             aaGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTATATTTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCaaa                                      <  2:9304741/150‑1 (MQ=255)
                                                                                                              aGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAt                                     <  1:161594/150‑1 (MQ=255)
                                                                                                              aGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAt                                     <  1:161009/150‑1 (MQ=255)
                                                                                                                gtcgtcGGTTCAAATCCGGCCCCCGCAACCAATTATCCTTTGTTGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAAttt                                   <  2:7303500/150‑1 (MQ=255)
                                                                                                                  cgtcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtga                                 <  2:2778766/150‑1 (MQ=255)
                                                                                                                   gtcgGTTCCAATCCGGCCCCCGCAACCAATTACAATTTGATGAAGT‑AAAGGAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtgat                                <  2:8834700/150‑1 (MQ=255)
                                                                                                                   gtcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtgat                                <  2:4528949/150‑1 (MQ=255)
                                                                                                                    tcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTtgagg                               >  1:4595849/1‑148 (MQ=255)
                                                                                                                    tcgGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAtaacaac                               >  2:6632650/1‑144 (MQ=255)
                                                                                                                      ggTTCAAATCCGGCCCCCGCAACCAATTATATTTTGTTGTAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGaa                             <  1:2482048/150‑1 (MQ=255)
                                                                                                                      ggTTCAAATCCGGCCCCCGCAAACAAATAAACATTGTTGAAGT‑CACCCACTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGaa                             <  1:4140205/150‑1 (MQ=17)
                                                                                                                       gTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAg                            <  1:9007988/150‑1 (MQ=255)
                                                                                                                         tCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTa                          >  1:2599800/1‑150 (MQ=255)
                                                                                                                           aaaTCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑ACAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGACGTCGGTTCAAATCCGGCCCCCGCAACCAATAAAAGTTGAAGAAGGaaa                        >  2:3920003/1‑150 (MQ=14)
                                                                                                                            aaTCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTaaaa                       <  2:6885252/150‑1 (MQ=255)
                                                                                                                              tCCGGCCCCCGGAACCAATTAAAATTTGATGAAGT‑AAAGCATTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGc                     <  2:4269927/150‑1 (MQ=255)
                                                                                                                              tCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATATGAAGAAGTAAAAGc                     >  2:8047655/1‑150 (MQ=255)
                                                                                                                                  gCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTa                 >  1:1929179/1‑150 (MQ=255)
                                                                                                                                         cAACCAATTATATTTTGTTGAAGT‑AATGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGAc          <  1:2935338/150‑1 (MQ=255)
                                                                                                                                         cAACCAATTACATTTTGTTGTAGT‑TTTGGAGTACTGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGAc          <  1:1007660/150‑1 (MQ=17)
                                                                                                                                            ccAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCATTAAGGTGAcgcg       >  1:2683237/1‑150 (MQ=255)
                                                                                                                                            ccAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGAcgcg       >  1:6095802/1‑150 (MQ=255)
                                                                                                                                                ttAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTTCGGTGACGCGGGGt   >  2:5863618/1‑150 (MQ=255)
                                                                                                                                                ttAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGt   <  2:5569953/150‑1 (MQ=255)
                                                                                                                                                ttAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGt   <  1:4859799/150‑1 (MQ=255)
                                                                                                                                                 tAAAATTTGATGAAGT‑AACGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTg  <  1:6652837/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATTAAAATTTGATGAAGT‑AAAGCAGTACGGTGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAATCAAATTTGATGAAGTAAAAGCAGTACGGTGACGCGGGGTG  >  NC_000913/2947321‑2947615

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑