BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,054,914	A→T	8.1%	T177T (ACT→ACA)	ygfB ←	UPF0149 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,054,914	0	A	T	8.1%	1658.9 / 12.3	532	T177T (ACT→ACA)	ygfB	UPF0149 family protein
Reads supporting (aligned to +/- strand): ref base A (157/312); new base T (41/2); total (218/314)
Fisher's exact test for biased strand distribution p-value = 2.99e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 556 total aligned reads displayed.

CGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCC  >  NC_000913/3054765‑3055063

cggcgCTGCCGGGGTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCGTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGa                                                                                                                                                       <  2:2121685/150‑1 (MQ=255)

    gcTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTaa                                                                                                                                                   <  2:7714524/150‑1 (MQ=255)

     cTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTaaa                                                                                                                                                  <  1:8823294/150‑1 (MQ=255)

       gCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTGTAGTGTAGAGTCGGTTTTTTTAATTCAGGCGCGGGCGGGTGCGGATGGGGAAAgg                                                                                                                                                >  1:8882004/1‑150 (MQ=255)

            gTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTATGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCg                                                                                                                                           >  2:4567662/1‑150 (MQ=255)

             ttGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTCTTCTGGCGCGGTCGGTTGCGGATGAGGAAAGGTGTCGt                                                                                                                                          >  2:1593850/1‑150 (MQ=255)

              tGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTCTTGCGCGGTCGGTTGCGGATGAGTAAAGGTGTAGTg                                                                                                                                         >  1:7146166/1‑150 (MQ=255)

              tGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTATTGCGCGGTCGGTTGCGGGTGAGTTAAGGTgtggtg                                                                                                                                         >  2:7478836/1‑150 (MQ=255)

              tGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTAATTATTGCGCGGTCGGGTGCGGTTGTGTAAAGgtttagtt                                                                                                                                         >  2:6911489/1‑144 (MQ=255)

              tGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTGGGTTGCGGATGAGTAAAGTTgtggtg                                                                                                                                         >  2:2043321/1‑150 (MQ=255)

              tGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTg                                                                                                                                         <  2:39608/150‑1 (MQ=255)

              tGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTg                                                                                                                                         <  1:3774493/150‑1 (MQ=255)

               gCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTgg                                                                                                                                        <  1:1502519/150‑1 (MQ=255)

                cATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGc                                                                                                                                       <  2:3803207/150‑1 (MQ=255)

                 aTTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACTTATTGCGCGGTGGGTTGCGGATGAGTAAAGGTGTCGTggga                                                                                                                                      >  1:6420265/1‑148 (MQ=255)

                 aTTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCa                                                                                                                                      <  1:4381257/150‑1 (MQ=255)

                  tttGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGGTGCGGCTGAGTAAAGGTGTCGTGGCAt                                                                                                                                     >  2:5577340/1‑150 (MQ=255)

                      cTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTCTTCTGGCGCGGGCGGGTGCGGGTGTGGAAAGGTGGCGTGGCATAACa                                                                                                                                 >  1:4601859/1‑150 (MQ=255)

                      cTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACa                                                                                                                                 <  2:542067/150‑1 (MQ=255)

                       tCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAAATCTGGCGCCGACGGATTCCGCTGAGTAACGGTGTCGTGGCAAAACac                                                                                                                                >  2:6063554/1‑149 (MQ=255)

                       tCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTGACGTGGTTTGTTTTTAGTGTAGAGTCGGGTTTTGGTCGTCTGGCGCGGGCGGGTGCGGGGGGGTTAAGGTGTGGGGGCCTAACAg                                                                                                                                >  2:8188981/1‑150 (MQ=255)

                         caccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTCTTATGGGGCGGTCGGTTTCGGATGAGTAAAGGTGTCGTGGCATAACAgcg                                                                                                                              >  1:122807/1‑150 (MQ=255)

                         caccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTACTTATGGCGCGGTGGGGTGCGGGTTTGTAAATGTTTGGGGGGATAACAgcc                                                                                                                              >  2:4300105/1‑149 (MQ=255)

                          accaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACTCTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAgcgc                                                                                                                             <  2:4983215/150‑1 (MQ=255)

                           ccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTATTCTGGCGCGGTCGGTGGCGGATGAGTAAAGGTGTCGTGGCATAAAAGcgcg                                                                                                                            >  1:3975633/1‑150 (MQ=255)

                           ccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGcgcg                                                                                                                            <  1:7889018/150‑1 (MQ=255)

                           ccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGcgcg                                                                                                                            <  2:1416832/150‑1 (MQ=255)

                           ccaGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGcgcg                                                                                                                            <  2:8002552/150‑1 (MQ=255)

                            caGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCgg                                                                                                                           <  2:216361/150‑1 (MQ=255)

                                gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAac                                                                                                                       <  2:8764323/150‑1 (MQ=255)

                                gCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAac                                                                                                                       <  1:2584542/150‑1 (MQ=255)

                                 ccTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                                                      <  2:2845323/150‑1 (MQ=255)

                                 ccTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAaca                                                                                                                      <  2:5865597/150‑1 (MQ=255)

                                     aCGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTATTAAGGCGCGGGCGGTTTCGGGTGTGGAAAGGTGTGGTGGGAAAACAGCGCCGCCACACGaa                                                                                                                  >  1:5865095/1‑150 (MQ=255)

                                     aCGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGaa                                                                                                                  <  1:1035605/150‑1 (MQ=255)

                                     aCGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGaa                                                                                                                  <  2:6806836/150‑1 (MQ=255)

                                      cGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAAc                                                                                                                 <  1:9415313/150‑1 (MQ=255)

                                         cGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACATATGGCGCGGTCGGGTGCGGCTGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACCAACCTa                                                                                                              >  2:5215541/1‑150 (MQ=255)

                                           aCGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTAtt                                                                                                            <  1:5629349/150‑1 (MQ=255)

                                             gCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTATTATTGCGCGGTGGGTTTCGGGGTTGTAAAGGTGTGGTGGGATAAAAACGCCGCAACACCAACGTAttag                                                                                                          >  1:9312136/1‑148 (MQ=255)

                                              cTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCga                                                                                                         <  1:2256645/150‑1 (MQ=255)

                                               tGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGGGCGGTCGGGTGCGGCTGAGTAAAGGGGTCGTGGCATAAAAGCGCGGCAACAAGCACGGATTAgat                                                                                                        >  2:2936698/1‑150 (MQ=255)

                                                gAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTTCTTCTGGCGCGGTCGGTTTCGGCTGAGTAAAGTTGTCGTGGCATAAAATCGCGGCAACACCAACGTAATCgagg                                                                                                       >  1:8664020/1‑148 (MQ=255)

                                                 aaaCTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCgatga                                                                                                      <  2:3506923/150‑1 (MQ=255)

                                                 aaaCTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTTGTTATGGCGCGGGGGGTTGCGGGTGTGTTAAGGTGTCGTTGGATAAAAGCGCGGCAAAACCAAACTATTAggtga                                                                                                      >  1:24683/1‑150 (MQ=255)

                                                  aaCTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCgatgat                                                                                                     <  2:1905132/150‑1 (MQ=255)

                                                     tctTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTATTGCGCGGTGGGTTGCGGTTGTGTAAAGGTGTGGTGGCATAACAGCGCGGCAACACCAAAGTATTCgatgatata                                                                                                  >  2:9195242/1‑147 (MQ=255)

                                                     tctTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTTTTCTTAAGGCGCGGGCGGGTGCGGGGGAGGAAATGTGTCGTGGCCAAAAAGCGCCGCAACACCAACCTAATAgatgatata                                                                                                  >  1:7377095/1‑147 (MQ=255)

                                                     tctTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTGGTGGCAAAACAGCGCGGCAACACCAACGTATTCGATGAtctc                                                                                                  >  2:1366006/1‑150 (MQ=255)

                                                          ccGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTACGTATGGCGCGGTCGGGTGCGGCTGAGTAAAGGTGTCGTGGGCGAACAGCGCGGCAACCCGCACCTATTCGATGGTCTCTTCaa                                                                                             >  2:8357769/1‑150 (MQ=255)

                                                             ggATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGGTTTTGTTATTCAGGCGCGGGCGGGTGCGGGTGTGTAAATGTGTCGTGGGCAAAAAGCGCGGCAAAACCAACGTATTAGATGGTGACTTAAAGcc                                                                                          >  1:532768/1‑149 (MQ=255)

                                                                 atCTCACACATAACACTCTCCTTACGATTTTTGATTTTAGTGTAGAGTCGGTTATTGTACTTCTCGCGCGGTCGGTTGCGGATGATTAAAGGTGTCGTCGCATAACAGCGCGGCAACACGAACGTATTCTATGATCTCTTAAAGCGACAt                                                                                      <  2:3000756/150‑1 (MQ=255)

                                                                      actcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCaa                                                                                 <  1:824544/150‑1 (MQ=255)

                                                                      actcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGGTGCGGTTGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCATCAAGCGACATTTaaa                                                                                 >  2:5711147/1‑150 (MQ=255)

                                                                        tcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTTTTATTGTTGCGCGGTCGGTTTGGGGGGAGTTAAGGGGTGGTGGCGTTAAAGCGCGGCAAACCCAACGTATTCGATGATGTCTTAAAGCGACATTTCAAGc                                                                               >  2:2531630/1‑150 (MQ=255)

                                                                        tcaTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCAGGCGCGGTCGGTTGCGGATGAGGAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGc                                                                               >  2:6352174/1‑150 (MQ=255)

                                                                          aTAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTATGGCGCGGTCGGTTTCGGGTGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATATCTTCAAGCGACAATTAAAGctc                                                                             >  2:4803404/1‑150 (MQ=255)

                                                                               acTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCt                                                                        <  2:8548836/150‑1 (MQ=255)

                                                                               acTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCt                                                                        <  2:8934654/150‑1 (MQ=255)

                                                                                  ctcCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAt                                                                     <  2:3400554/150‑1 (MQ=255)

                                                                                  ctcCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAt                                                                     <  2:3096558/150‑1 (MQ=255)

                                                                                   tcCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtc                                                                    <  1:4781970/150‑1 (MQ=255)

                                                                                   tcCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACATATGGCGCGGTCGGTTGCGGATGAGTAAATGTGTCGTGGCATAACAGCCCGGCAAAAAGAACCGATTAGATGATCTCTTCAAGCGACATTTAAAACAATACCTGAta                                                                    >  2:7106681/1‑149 (MQ=255)

                                                                                    ccTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtct                                                                   <  1:8097192/150‑1 (MQ=255)

                                                                                      ttACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttc                                                                 <  2:6601408/150‑1 (MQ=255)

                                                                                      ttACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttc                                                                 <  1:5412644/150‑1 (MQ=255)

                                                                                      ttACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttc                                                                 <  2:7251526/150‑1 (MQ=255)

                                                                                        aCGTTGTTTGTTTTTAGTGTAGAGTCGGTGTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGACCGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGAtcttcgt                                                               <  2:6176917/150‑1 (MQ=255)

                                                                                          gTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTAATTAAGGCGCCGTCGGTTTCGGATTAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTAGATGATATATTAAAGCGACATTTAAAGCTCATACTGAtattagtct                                                             >  2:3231391/1‑150 (MQ=255)

                                                                                              tttGTTTTTAGTGTAGAGTCGGTTTTTTTTATTCTGGCGCGGTGGGTTGCGGGTGTTTTAAGGTGTGGTGGCATAAAAGCGCGGCAACACGAACGTATTCGATGATCTCTTAAAGCGACATTTCAAGCTCATCCAGATcttagtgttcgt                                                         >  2:1962462/1‑150 (MQ=255)

                                                                                                 gTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTAGTCTTcgtcgt                                                      <  1:3555999/150‑1 (MQ=255)

                                                                                                  tttttAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTa                                                     <  2:1875097/150‑1 (MQ=255)

                                                                                                       aGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCa                                                <  1:7637223/150‑1 (MQ=255)

                                                                                                          gtAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGtt                                             <  2:2322605/150‑1 (MQ=255)

                                                                                                            agagTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTgc                                           <  2:7827927/150‑1 (MQ=255)

                                                                                                             gagTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTgcg                                          <  1:8417441/150‑1 (MQ=255)

                                                                                                                tCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCaa                                       >  2:4051946/1‑150 (MQ=255)

                                                                                                                   gTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGt                                    >  1:9188758/1‑150 (MQ=255)

                                                                                                                   gTTTTTGAACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGt                                    >  2:7953267/1‑150 (MQ=255)

                                                                                                                     ttttGTACGTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTa                                  <  2:368039/150‑1 (MQ=255)

                                                                                                                      tttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTAc                                 <  1:4944512/150‑1 (MQ=255)

                                                                                                                      tttGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTAc                                 <  1:3428773/150‑1 (MQ=255)

                                                                                                                        tGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGc                               >  1:3713130/1‑150 (MQ=255)

                                                                                                                        tGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGc                               <  1:1950800/150‑1 (MQ=255)

                                                                                                                             ttCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCCATGTTACGCAGaac                          >  1:6281581/1‑148 (MQ=255)

                                                                                                                             ttCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGAtc                          >  2:5984230/1‑150 (MQ=255)

                                                                                                                             ttCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGAtc                          >  1:6977763/1‑150 (MQ=255)

                                                                                                                               cTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGAtcgt                        >  2:9585795/1‑150 (MQ=255)

                                                                                                                                tGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGAtcgtc                       <  2:8846944/150‑1 (MQ=255)

                                                                                                                                    gcgGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCGAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATa                   <  1:6684811/150‑1 (MQ=255)

                                                                                                                                    gcgGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATa                   <  2:7123866/150‑1 (MQ=255)

                                                                                                                                           gtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCTAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCAc            <  1:1631842/150‑1 (MQ=255)

                                                                                                                                           gtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCAc            <  1:9054885/150‑1 (MQ=255)

                                                                                                                                           gtTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCAc            <  2:2355924/150‑1 (MQ=255)

                                                                                                                                            ttGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCAcc           <  2:171392/150‑1 (MQ=255)

                                                                                                                                              gCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCgg         >  2:1097475/1‑150 (MQ=255)

                                                                                                                                                ggaggaGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGtt       >  1:4263005/1‑150 (MQ=255)

                                                                                                                                                ggATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGtt       >  1:322109/1‑150 (MQ=255)

                                                                                                                                                     aGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGcc  >  2:7377544/1‑150 (MQ=255)

                                                                                                                                                     aGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGcc  <  1:2912796/150‑1 (MQ=255)

CGGCGCTGCCGGGTTGCATTTGCTCCACCAGGGCCTGACGGCGACGCTGAAACTCTTGCCGGGATATCTCACTCATAACACTCTCCTTACGTTTTTTGTTTTTAGTGTAGAGTCGGTTTTTGTACTTCTGGCGCGGTCGGTTGCGGATGAGTAAAGGTGTCGTGGCATAACAGCGCGGCAACACGAACGTATTCGATGATCTCTTCAAGCGACATTTCAAGCTCTTCCTGATCTTCGTCTTCGTCGTAACCCAGTTGCGCAATGTTACGCAGATCGTCGATAGCTTCACCGGTTTCGCC  >  NC_000913/3054765‑3055063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`