Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,455,680 G→T 5.3% V35L (GTG→TTG)  gspC → general secretory pathway component, cryptic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,455,6800GT 5.3% 1465.6 / 8.2 450V35L (GTG→TTG) gspCgeneral secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base G (188/222);  new base T (38/2);  total (226/224)
Fisher's exact test for biased strand distribution p-value = 3.25e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 461 total aligned reads displayed.

AACGTCAATATACAACCCACCCAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATGCAAATCCCGTGGCAGTTAATCAG  >  NC_000913/3455531‑3455829
                                                                                                                                                     |                                                                                                                                                     
  cGTCAATATACAACCCACCCAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAAtgtg                                                                                                                                                     <  1:5277789/150‑1 (MQ=255)
   gTCAATATACAACCCACCCAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGa                                                                                                                                                    <  1:5128389/150‑1 (MQ=255)
     cAATATACAACCCACCCAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAat                                                                                                                                                  >  1:6832391/1‑150 (MQ=255)
      aaTATACAACCCACCCAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAatt                                                                                                                                                 >  2:8411931/1‑150 (MQ=255)
       atatACAACCCACCCAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAatta                                                                                                                                                >  1:4098428/1‑150 (MQ=255)
          taCAACCCACCCAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATTTGAATTAttt                                                                                                                                             >  1:4428216/1‑150 (MQ=255)
          taCAACCCACCCAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATTTGAATTAttt                                                                                                                                             >  1:834392/1‑150 (MQ=255)
          taCAACCCACCCAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTAttt                                                                                                                                             <  2:3030962/150‑1 (MQ=255)
           aCAACCCACCCAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTc                                                                                                                                            <  1:1702204/150‑1 (MQ=255)
             aaCCCACCCAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCa                                                                                                                                          <  2:8577554/150‑1 (MQ=255)
             aaCCCACCCAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCa                                                                                                                                          <  2:2514587/150‑1 (MQ=255)
             aaCCCACCCAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCa                                                                                                                                          <  2:6122573/150‑1 (MQ=255)
              acccacccAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCAt                                                                                                                                         <  1:1832948/150‑1 (MQ=255)
              acccacccAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCAt                                                                                                                                         <  1:3705408/150‑1 (MQ=255)
               cccacccaATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATa                                                                                                                                        >  1:9445406/1‑150 (MQ=255)
                   cccaATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATTTGTATTATTTCCATaaaaa                                                                                                                                    >  1:8581366/1‑149 (MQ=255)
                     caATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAAcaa                                                                                                                                  >  1:1205681/1‑148 (MQ=255)
                     caATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACta                                                                                                                                  >  1:6206530/1‑150 (MQ=255)
                     caATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTACATAAAACta                                                                                                                                  >  1:1374274/1‑150 (MQ=255)
                            ttttATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATTTGAATTTTTTGCATAAAAATTTAGTTaa                                                                                                                           >  2:2729330/1‑150 (MQ=255)
                                attaGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAAt                                                                                                                       <  1:2553990/150‑1 (MQ=255)
                                attaGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAAt                                                                                                                       <  2:1176710/150‑1 (MQ=255)
                                 ttaGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAAATATTTACATAAAAATAAAGTTAAAAATg                                                                                                                      >  2:4167851/1‑150 (MQ=255)
                                  taGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATgg                                                                                                                     >  2:4775682/1‑150 (MQ=255)
                                   aGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGTATTATTTACATTCAACTATATTTAAAAATTGa                                                                                                                    >  2:9295004/1‑150 (MQ=255)
                                    gAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGAc                                                                                                                   <  2:7123972/150‑1 (MQ=255)
                                     aaCATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTACATAAAACTATAGTTAAAAATGGACa                                                                                                                  >  1:2053344/1‑150 (MQ=255)
                                     aaCATACATCGTGCCCACACTACGTTTCCCCTTCCAACTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAAATAAATCCAAACAACTATACTTAAAAAAGGACa                                                                                                                  >  2:1549260/1‑150 (MQ=255)
                                      acatacatCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAg                                                                                                                 <  2:6831356/150‑1 (MQ=255)
                                        atacatCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGTATTATTTACATACAAATATAGTTAAAAATGGACAGAt                                                                                                               >  1:8152063/1‑150 (MQ=255)
                                        atacatCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGAt                                                                                                               <  1:4210108/150‑1 (MQ=255)
                                          acatCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCa                                                                                                             <  1:6129947/150‑1 (MQ=255)
                                           catCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCAATACAAATATATTTAAAAATGGAAAGATAAt                                                                                                            >  2:4956718/1‑150 (MQ=255)
                                               gTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAAt                                                                                                        <  1:5523590/150‑1 (MQ=255)
                                                  cccACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATcaa                                                                                                     <  1:5836710/150‑1 (MQ=255)
                                                   ccACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATCTTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATcaac                                                                                                    <  2:9459325/150‑1 (MQ=255)
                                                     acacTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTTATTTGAATTATTTACATACAAATATAGTTAAAAATTGACAGATCAGTAATaaacca                                                                                                  >  1:9029531/1‑150 (MQ=255)
                                                     acacTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATTTGTATTATTTACATACAACAATATTTAAAAATGGTCAGATCATTAATcaacca                                                                                                  >  1:9191909/1‑150 (MQ=255)
                                                      cacTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATTTGAATTATTTAAATACAACAATAGTTAAAAATGGACAGATAATTAAAcaaccaa                                                                                                 >  1:5346674/1‑150 (MQ=255)
                                                             tttCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATTTTAATTATTTACATAAAACTATAGTTAAAAATGGACAGATAATTAATCAAACAAAAAAAGc                                                                                          >  1:9132952/1‑150 (MQ=255)
                                                                   cTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACGATAGTTAAAAATGGACAGATAATTAATCAACCAACAAAAGCATTTCa                                                                                    >  2:2245378/1‑150 (MQ=255)
                                                                   cTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACAATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCAATTCa                                                                                    >  2:1748282/1‑150 (MQ=255)
                                                                         tCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGa                                                                              >  2:9663154/1‑150 (MQ=255)
                                                                            ggCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGAttt                                                                           <  1:3410889/150‑1 (MQ=255)
                                                                             gCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACAATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTc                                                                          >  2:5897777/1‑150 (MQ=255)
                                                                              cGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCa                                                                         <  2:4317910/150‑1 (MQ=255)
                                                                              cGAAACATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTTAATTGAATTAGTTACAAAAAACGATATGTAAAAAATGTCCGGTAAATAATCAAACAACAAAACCATTTAAGACAGAATTCa                                                                         >  1:6591068/1‑150 (MQ=255)
                                                                               gAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAg                                                                        >  2:8737159/1‑150 (MQ=255)
                                                                               gAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTAGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAg                                                                        <  2:7790363/150‑1 (MQ=255)
                                                                                   cATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATTTGAATTATTTACATACAACTATATTTAAAAATTGACAGATCATTAATAAAACAACAAAAGCATTTAAGTCTGATTTAAGccgt                                                                    >  1:6665215/1‑148 (MQ=255)
                                                                                   cATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCtt                                                                    <  2:5135390/150‑1 (MQ=255)
                                                                                    ataataAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTTATTTGAATTATTTGAAAAAAACAATAGTTTAAAATTGACAGATCATTAATAAAACAAAAAACCCCTTTAAGTCTGATTTAAGCCTTg                                                                   >  1:3307647/1‑150 (MQ=255)
                                                                                     taataaAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGc                                                                  <  1:3009826/150‑1 (MQ=255)
                                                                                     taataaAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGc                                                                  <  1:3903635/150‑1 (MQ=255)
                                                                                     taataaAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCAGTTCAGTCTGATTTCAGCCTTGc                                                                  >  2:6199566/1‑150 (MQ=255)
                                                                                     taataaAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAAAGGACAGATAATTAATCAACCAACAAAAGCATTTCAGTCTGATTTCAGCCATGc                                                                  >  2:2144724/1‑150 (MQ=255)
                                                                                       ataaAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAAAGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCGTGCCg                                                                >  2:1897975/1‑150 (MQ=255)
                                                                                        taaAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGc                                                               <  1:7420310/150‑1 (MQ=255)
                                                                                          aaGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCtt                                                             <  2:1368777/150‑1 (MQ=255)
                                                                                             aTGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTAt                                                          <  1:848131/150‑1 (MQ=255)
                                                                                              tgctgcCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATg                                                         <  1:1445522/150‑1 (MQ=255)
                                                                                              tgctgcCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATg                                                         <  1:1065958/150‑1 (MQ=255)
                                                                                                ctgcCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATAAAACTATAGTTAAAAATTGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGc                                                       >  2:4654968/1‑150 (MQ=255)
                                                                                                 tgcCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATTGACAGATCATTAATAAACCAACAAAAGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCg                                                      >  2:128970/1‑150 (MQ=255)
                                                                                                 tgcCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTACATAAAACTATATTTAAAAATTGACAGATAATTAATAAACCAACAAACGCATTTAAGTATTATTTCAGCCTTGCCGCTTTATTGCg                                                      >  2:7639234/1‑150 (MQ=255)
                                                                                                   ccATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTa                                                    <  1:7810977/150‑1 (MQ=255)
                                                                                                     aTCTATATATTGATTATATTTATAAGCATAGGTTCTATTATTTTTTATGTGAATTATTTCCATACAACTATAGTTAAAAATTGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAAc                                                  <  2:6381326/150‑1 (MQ=255)
                                                                                                     aTCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAAc                                                  >  2:6305995/1‑150 (MQ=255)
                                                                                                            tatTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTACATACAAAAATAGTTAAAAAATGACAGATAATTAATAAAACAACAAAAGCATTTCAGTCTTATTTAAGCCATGCCCCTTTATTGCGTAACGAAAAta                                           >  2:828975/1‑149 (MQ=255)
                                                                                                               tGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATg                                        <  2:7843804/150‑1 (MQ=255)
                                                                                                               tGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATg                                        <  1:4628718/150‑1 (MQ=255)
                                                                                                                gATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATATTTAAAAATGGACAGATCATTAATCAAACAACAAACGCATTTCAGTCTTATTTAATCCTTTCCGCTTTATGGCGTAACGAAaataattc                                       >  2:3759170/1‑148 (MQ=255)
                                                                                                                  ttATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTg                                     >  2:9517209/1‑150 (MQ=255)
                                                                                                                   tataTTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTgg                                    <  1:577406/150‑1 (MQ=255)
                                                                                                                     tatTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTgggg                                  <  1:2300390/150‑1 (MQ=255)
                                                                                                                      atTTATAAGCATAGGGTCTATTATATATAATGTGAATTATTTCCATACAACAATACTTAAAAATGGACAGATCATTAATCAACCAACAAACCCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGt                                 <  2:6599669/150‑1 (MQ=255)
                                                                                                                       tttATAAGCATAGGGTCGATTATTTTTAATGTGAATTATATCCAAAAAACTAAAGTTAAAAATGGACAGATAATTAATCAACCAACAAACGCATTTAAGTCTGATTTAAGCCTTGCCGCTTTATTGCGTAAAGAAAAAAATGCTGGGGTa                                >  2:5964704/1‑150 (MQ=255)
                                                                                                                          ataAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTaaaa                             <  1:9295004/150‑1 (MQ=255)
                                                                                                                          ataAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCAATAATCAACCAACAAACGCAATTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTaaaa                             >  2:5357232/1‑150 (MQ=255)
                                                                                                                            aaGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGa                           <  1:6425224/150‑1 (MQ=255)
                                                                                                                             aGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGAt                          <  1:9707102/150‑1 (MQ=255)
                                                                                                                             aGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGAt                          <  2:4051831/150‑1 (MQ=255)
                                                                                                                             aGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGAt                          <  2:5250197/150‑1 (MQ=255)
                                                                                                                              gCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATTGACAGATAATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATTGCGTAACGAAAATCAAGCTGGGGTAAAAGATg                         >  2:6727540/1‑150 (MQ=255)
                                                                                                                              gCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATg                         <  1:7269911/150‑1 (MQ=255)
                                                                                                                               cATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATGc                        <  2:3242527/150‑1 (MQ=255)
                                                                                                                                     gTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATGCAAATcc                  >  2:4871884/1‑150 (MQ=255)
                                                                                                                                     gTCGATTATTTTTAATGAGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATGCAAATcc                  <  2:1962255/150‑1 (MQ=255)
                                                                                                                                        gATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGAt                          <  1:4480193/139‑1 (MQ=255)
                                                                                                                                        gATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGAt                          >  2:4480193/1‑139 (MQ=255)
                                                                                                                                        gATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATGCAAATCCCGt               <  2:8067446/150‑1 (MQ=255)
                                                                                                                                        gATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATGCAAATCCCGt               <  2:3862580/150‑1 (MQ=255)
                                                                                                                                         attattTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATGCAAATCCCGTg              <  2:6698679/150‑1 (MQ=255)
                                                                                                                                          ttattTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATGCAAATCCCGTgg             >  1:4164163/1‑150 (MQ=255)
                                                                                                                                           tattTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTATCGAAAATCATGCTGGGGTAAAAGATGCAAATCCCGTGGc            <  2:7391248/150‑1 (MQ=255)
                                                                                                                                            attTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATGCAAATCCCGTGGCa           <  2:335080/150‑1 (MQ=255)
                                                                                                                                              ttttAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATGCAAATCCCGTGGCAGt         <  1:4956264/150‑1 (MQ=255)
                                                                                                                                                  aaTGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATGCAAATCCCGTGGCAGTTAAt     >  2:6945931/1‑150 (MQ=255)
                                                                                                                                                   aTGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATGCAAATCCCGTGGCAGTTAATc    <  2:9029531/150‑1 (MQ=255)
                                                                                                                                                   aTGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATGCAAATCCCGTGGCAGTTAATc    <  2:5828042/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACGTCAATATACAACCCACCCAATCTATTTTATTAGAACATACATCGTGCCCACACTACGTTTCCCCTTCCATCTGGCGAATCATAATAAAGATGCTGCCATCAATATATTGATTATATTTATAAGCATAGGGTCGATTATTTTTAATGTGAATTATTTCCATACAACTATAGTTAAAAATGGACAGATCATTAATCAACCAACAAACGCATTTCAGTCTGATTTCAGCCTTGCCGCTTTATGGCGTAACGAAAATCATGCTGGGGTAAAAGATGCAAATCCCGTGGCAGTTAATCAG  >  NC_000913/3455531‑3455829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑