Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,482,325 G→A 100% R346H (CGC→CAC)  yheS → ABC‑F family protein predicted regulatory ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,482,3250GA99.7% 2402.7 / ‑3.7 628R346H (CGC→CAC) yheSABC‑F family protein predicted regulatory ATPase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (282/344);  total (283/345)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 629 total aligned reads displayed.

GTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTA  >  NC_000913/3482179‑3482471
                                                                                                                                                  |                                                                                                                                                  
gTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCACGCGTATTGGTCTGTTAGGCCACaa                                                                                                                                                 >  1:5697809/1‑150 (MQ=255)
 ttCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCcacact                                                                                                                                                >  2:6957750/1‑148 (MQ=255)
 ttCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAAt                                                                                                                                                >  2:2089119/1‑150 (MQ=255)
  tCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATg                                                                                                                                               <  1:6083091/150‑1 (MQ=255)
  tCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATg                                                                                                                                               <  1:395949/150‑1 (MQ=255)
      cTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGgcgc                                                                                                                                           >  2:1037903/1‑150 (MQ=255)
      cTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGgcgc                                                                                                                                           <  2:1674154/150‑1 (MQ=255)
       tttAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGcgcg                                                                                                                                          >  1:7749498/1‑150 (MQ=255)
        ttAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCgg                                                                                                                                         >  2:3474464/1‑150 (MQ=255)
        ttAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCgg                                                                                                                                         <  1:3471083/150‑1 (MQ=255)
            cTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTaa                                                                                                                                     >  2:5306427/1‑150 (MQ=255)
             ttCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTaaa                                                                                                                                    >  2:682396/1‑150 (MQ=255)
             ttCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTaaa                                                                                                                                    >  2:3626890/1‑150 (MQ=255)
              tCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTCTGGCGATCGCATTATTCTCGACTCGATTCAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACACTGGCGCGGGGAAAt                                                                                                                                   >  1:6640002/1‑150 (MQ=255)
              tCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAAt                                                                                                                                   <  2:3592354/150‑1 (MQ=255)
              tCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAAt                                                                                                                                   <  2:7597073/150‑1 (MQ=255)
               ccGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATc                                                                                                                                  <  2:8338323/150‑1 (MQ=255)
                 gcgcgcCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGa                                                                                                                                <  1:3834256/150‑1 (MQ=255)
                 gcgcgcCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGa                                                                                                                                <  2:4333694/150‑1 (MQ=255)
                  cgcgcCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGAc                                                                                                                               <  1:2041232/150‑1 (MQ=255)
                  cgcgcCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGAc                                                                                                                               <  1:8103515/150‑1 (MQ=255)
                      ccGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGTGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTa                                                                                                                           >  1:5170013/1‑150 (MQ=255)
                      ccGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTa                                                                                                                           >  1:3325395/1‑150 (MQ=255)
                            aGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCaaa                                                                                                                     <  2:3760340/150‑1 (MQ=255)
                            aGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCaaa                                                                                                                     <  2:4508541/150‑1 (MQ=255)
                             gCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAAc                                                                                                                    >  2:4123482/1‑150 (MQ=255)
                             gCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCAACATTAATCAAAc                                                                                                                    <  2:3025822/150‑1 (MQ=255)
                                tGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTaa                                                                                                                                     >  1:1184865/1‑130 (MQ=255)
                                 gCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGtt                                                                                                                <  2:1425121/150‑1 (MQ=255)
                                   cAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAg                                                                                                              <  1:9512299/150‑1 (MQ=255)
                                    aaaTCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGc                                                                                                             >  2:9232271/1‑150 (MQ=255)
                                         cGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTg                                                                                                        >  1:8958463/1‑150 (MQ=255)
                                         cGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTg                                                                                                        <  2:4028259/150‑1 (MQ=255)
                                         cGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTg                                                                                                        <  2:9443420/150‑1 (MQ=255)
                                          gTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGa                                                                                                       >  2:2427323/1‑150 (MQ=255)
                                           ttACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGaa                                                                                                      >  2:7226368/1‑150 (MQ=255)
                                           ttACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGaa                                                                                                      <  1:25729/150‑1 (MQ=255)
                                           ttACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAAACGACATTAATCAAACAGATAGCCGGTGaa                                                                                                      >  2:7334801/1‑150 (MQ=255)
                                               tGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTg                                                                                                  >  2:6384726/1‑150 (MQ=255)
                                                gAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTgc                                                                                                 >  2:4963640/1‑150 (MQ=255)
                                                gAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTAAGCCGGTGAACTTgc                                                                                                 >  1:5299487/1‑150 (MQ=255)
                                                    aTGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCa                                                                                             >  2:8044509/1‑150 (MQ=255)
                                                      ggAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGt                                                                                           >  1:2149439/1‑150 (MQ=255)
                                                        aaaaaGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCa                                                                                         >  1:2926300/1‑150 (MQ=255)
                                                        aaaaaGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCa                                                                                         <  1:7554972/150‑1 (MQ=255)
                                                                  cgcgGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAAt                                                                               <  1:6224477/150‑1 (MQ=255)
                                                                   gcgGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAAtt                                                                              <  1:5861422/150‑1 (MQ=255)
                                                                    cgGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTg                                                                             <  2:8640219/150‑1 (MQ=255)
                                                                    cgGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTg                                                                             <  2:223457/150‑1 (MQ=255)
                                                                     gggCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTgg                                                                            >  1:1031524/1‑150 (MQ=255)
                                                                     gggCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTgg                                                                            <  2:1248713/150‑1 (MQ=255)
                                                                        cTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCt                                                                         <  2:3179057/150‑1 (MQ=255)
                                                                         tATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTg                                                                        >  1:4112827/1‑150 (MQ=255)
                                                                          aTGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTgg                                                                       <  2:5054606/150‑1 (MQ=255)
                                                                             gCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGa                                                                    >  1:654627/1‑150 (MQ=255)
                                                                             gCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGa                                                                    >  1:5142695/1‑150 (MQ=255)
                                                                             gCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGa                                                                    >  1:3101454/1‑150 (MQ=255)
                                                                               gATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGaaa                                                                  >  1:2283712/1‑150 (MQ=255)
                                                                               gATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGaaa                                                                  <  1:4413523/150‑1 (MQ=255)
                                                                                   gCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGa                                                              <  1:6963622/150‑1 (MQ=255)
                                                                                    cATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGAt                                                             >  1:793854/1‑150 (MQ=255)
                                                                                     attattCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATc                                                            <  1:4689672/150‑1 (MQ=255)
                                                                                      ttattCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCa                                                           >  1:1755941/1‑150 (MQ=255)
                                                                                             cgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCgg                                                    <  1:8504607/150‑1 (MQ=255)
                                                                                             cgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCgg                                                    <  2:6133184/150‑1 (MQ=255)
                                                                                             cgactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCgg                                                    <  2:343828/150‑1 (MQ=255)
                                                                                              gactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGc                                                   >  2:4257100/1‑150 (MQ=255)
                                                                                              gactcgaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGc                                                   <  1:1906678/150‑1 (MQ=255)
                                                                                                   gaTTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACtt                                              >  2:8879579/1‑150 (MQ=255)
                                                                                                     ttAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCg                                            <  2:7749069/150‑1 (MQ=255)
                                                                                                       aaaCTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGcc                                          <  1:6761248/150‑1 (MQ=255)
                                                                                                                cTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCaa                                 >  1:2715569/1‑150 (MQ=255)
                                                                                                                cTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCaa                                 <  1:4374708/150‑1 (MQ=255)
                                                                                                                cTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCaa                                 >  2:8148887/1‑150 (MQ=255)
                                                                                                                 tGGTGCCCGGCTCGCGTTTTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAAc                                <  1:7334801/150‑1 (MQ=255)
                                                                                                                 tGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAAc                                <  1:9313157/150‑1 (MQ=255)
                                                                                                                       ccGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAAt                          >  1:8706029/1‑150 (MQ=255)
                                                                                                                       ccGGCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAAt                          >  1:1551853/1‑150 (MQ=255)
                                                                                                                         ggCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATAc                        >  1:960030/1‑150 (MQ=255)
                                                                                                                          gCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATAcc                       <  2:5439729/150‑1 (MQ=255)
                                                                                                                          gCTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAAACGAAATTAATCAAACTGTTAGCCGGTGAACTATCGCCAGTCAGCGGACAAATAGGTCTGGCGAAAGGGATCAAACTCGGCAACTACGCCCAGCATCAACTTGAATAca                       >  2:7853170/1‑149 (MQ=255)
                                                                                                                           cTCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCt                      <  2:658125/150‑1 (MQ=255)
                                                                                                                            tCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTg                     <  1:5384552/150‑1 (MQ=255)
                                                                                                                            tCGCGTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTg                     >  1:7881122/1‑150 (MQ=255)
                                                                                                                             cgcgTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTgc                    <  1:3552985/150‑1 (MQ=255)
                                                                                                                             cgcgTATTGGTCTGTTAGCCCACAATGGCGCGGGTAAATCGACAATAATCAAACTGTTAGCCGGTGAACTAGCGCCAGACAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGACCAGCATCAACTTGAATACCTgc                    <  2:906536/150‑1 (MQ=255)
                                                                                                                               cgTATTGGTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTgcgc                  >  1:920833/1‑150 (MQ=255)
                                                                                                                                     ggTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgac            >  1:3690293/1‑150 (MQ=255)
                                                                                                                                      gTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgacg           >  1:2877735/1‑150 (MQ=255)
                                                                                                                                      gTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgacg           >  1:500775/1‑150 (MQ=255)
                                                                                                                                      gTCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgacg           >  1:1403938/1‑150 (MQ=255)
                                                                                                                                       tCTGTTAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgacga          >  1:2404421/1‑150 (MQ=255)
                                                                                                                                           ttAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCcgacga          <  2:27454/146‑1 (MQ=255)
                                                                                                                                           ttAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCa      <  1:6449969/150‑1 (MQ=255)
                                                                                                                                            tAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCAc     <  1:4282593/150‑1 (MQ=255)
                                                                                                                                            tAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCAc     <  1:4604207/150‑1 (MQ=255)
                                                                                                                                            tAGGCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCAc     <  1:5624039/150‑1 (MQ=255)
                                                                                                                                              ggCCACAATGGCGCGGGTAAATCGACATTAATCACACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCt   <  2:5704715/150‑1 (MQ=255)
                                                                                                                                               gCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTa  >  2:578622/1‑150 (MQ=255)
                                                                                                                                               gCCACAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTa  <  1:4878321/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTTCCGCTTTAGCTTCCGCGCGCCGGAAAGCCTGCCAAATCCGTTACTGAAGATGGAAAAAGTCAGCGCGGGCTATGGCGATCGCATTATTCTCGACTCGATTAAACTGAACCTGGTGCCCGGCTCGCGTATTGGTCTGTTAGGCCGCAATGGCGCGGGTAAATCGACATTAATCAAACTGTTAGCCGGTGAACTTGCGCCAGTCAGCGGTGAAATTGGTCTGGCGAAAGGGATCAAACTCGGCTACTTCGCCCAGCATCAACTTGAATACCTGCGCGCCGACGAATCACCTA  >  NC_000913/3482179‑3482471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: