Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,407,890 Δ1 bp 5.3% coding (330/1218 nt) alaA → glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,407,8900G. 5.3% 390.6 / 16.6 132coding (330/1218 nt)alaAglutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase
Reads supporting (aligned to +/- strand):  ref base G (64/57);  new base . (5/2);  total (71/61)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 136 total aligned reads displayed.

AACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATG  >  NC_000913/2407741‑2408038
                                                                                                                                                     |                                                                                                                                                     
aaCCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCCGCACGACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAg                                                                                                                                                       >  1:1026554/1‑150 (MQ=255)
aaCCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAg                                                                                                                                                       >  2:66933/1‑150 (MQ=255)
aaCCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAg                                                                                                                                                       >  2:1438264/1‑150 (MQ=255)
  ccTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGc                                                                                                                                                     <  1:769435/150‑1 (MQ=255)
      ccTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGTAGCTTATCGTTCAGGCAATg                                                                                                                                                 >  2:1317246/1‑150 (MQ=255)
         aCCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTATAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCATTGGTGTATCGGCGCTTATCGTTCAGGCAATGCAg                                                                                                                                              >  1:239321/1‑150 (MQ=255)
               cAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTg                                                                                                                                        >  1:226104/1‑150 (MQ=255)
                acgcgTATTGCGAATCCAAAGGTCTTTCCTCCGCGCGTATAGCCATCATCCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACCTCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGc                                                                                                                                      <  2:120595/146‑1 (MQ=255)
                 aGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCt                                                                                                                                     <  2:1875904/150‑1 (MQ=255)
                 aGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGATCAGGCAATGCAGGCATTGCt                                                                                                                                     >  1:1731362/1‑150 (MQ=255)
                     aaatGCGAGTCCAAAGGTCTCTACGCCGCGCGTAACGCCATCCTGCAGCACTACCAGGCTCGTGGCAGGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGCAATGCCTGCATTGCCTGAAc                                                                                                                                 <  2:907814/147‑1 (MQ=255)
                        ttcGATTCCAAAGGTCTTAACTCCGCGCGTAAAGCCATCATGCCGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAAAGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGc                                                                                                                              <  1:1622203/148‑1 (MQ=255)
                         gCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCg                                                                                                                             >  1:1112118/1‑150 (MQ=255)
                         gCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCg                                                                                                                             >  2:35800/1‑150 (MQ=255)
                          cGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCgg                                                                                                                            <  1:1171042/150‑1 (MQ=255)
                           gATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCggg                                                                                                                           <  1:168862/150‑1 (MQ=255)
                              tCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGAc                                                                                                                        >  1:10182/1‑150 (MQ=255)
                               ccAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACg                                                                                                                       >  2:883323/1‑150 (MQ=255)
                                       cTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTg                                                                                                               <  1:1664568/150‑1 (MQ=255)
                                          tACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGATACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGtt                                                                                                            <  1:1190387/150‑1 (MQ=255)
                                            cTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTAGCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCGGAACAGCGGGGACGAAATGTTGGTTcc                                                                                                          <  2:261294/150‑1 (MQ=255)
                                            cTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGGGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTcc                                                                                                          <  1:1323177/150‑1 (MQ=255)
                                            cTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGCGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTcc                                                                                                          >  1:1364389/1‑150 (MQ=255)
                                              ccGCGCGTAAAGCCATCATGCAGCACTCCCAGGCTCGTGGCATGCGTGATGTTACCGGGGAAGCTTTTTAGATCGGCAATGGGGTATCTGAGCTTATCGTTCAGGCATTGCAGGCTTTGCTGAACAGCGGGGCCGCATTGTGGGTTCCTg                                                                                                        >  2:946864/1‑150 (MQ=255)
                                              ccGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCATGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTg                                                                                                        >  2:958279/1‑150 (MQ=255)
                                              ccGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTg                                                                                                        >  1:836480/1‑150 (MQ=255)
                                              ccGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTg                                                                                                        <  2:1489065/150‑1 (MQ=255)
                                               cgcgcgTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATACTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGc                                                                                                       >  2:443201/1‑150 (MQ=255)
                                                gcgcgTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTAACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGGGCTTATCGTTCACGCAATGCACTCATTGCTGAACAGCCGGGCCGAAATCTTGGTTCCTGcc                                                                                                      >  1:118622/1‑149 (MQ=255)
                                                 cgagTAAAGCCAGCATGCAGCACTACCAGTCTCGGGGCATGCGGGATGTTACCGTTGAATATATTTACATCGTCAATGTTGTATAGGAGATTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCAc                                                                                                     <  2:563526/147‑1 (MQ=255)
                                                   cgTAAAGCCATCATGGAGCACTAGCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGGATCGGAGCGTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGGACCa                                                                                                   <  1:984472/150‑1 (MQ=255)
                                                    gTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAg                                                                                                  <  2:878699/150‑1 (MQ=255)
                                                     tAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGa                                                                                                 <  1:332325/150‑1 (MQ=255)
                                                      aaaGCCAGCATGCAGCACTACCAGGCTCGTGGCTTGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGAt                                                                                                <  1:320895/150‑1 (MQ=255)
                                                        aGCCATAATGCAGCACTACCAGGCTCGTGGCTTGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGAGTa                                                                                              >  2:1634540/1‑150 (MQ=255)
                                                         gCCAGCATGCAGCACTACCAGGCTCGTGTCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCGTATCGTTCAGGCAATGCAGGCAGTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAc                                                                                             <  1:291088/150‑1 (MQ=255)
                                                          ccATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATAGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAcc                                                                                            >  1:151373/1‑150 (MQ=255)
                                                           catcatGCAGCACTACCAGGCTCGTGGCTTGCGTGATGTTACCGTGGAAGATAATTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGATCCTGCACCAGATTAccc                                                                                           >  1:1341138/1‑150 (MQ=255)
                                                           catcatGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAccc                                                                                           >  1:955184/1‑150 (MQ=255)
                                                              catGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATTGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATAGATGAACAGCGGGAACGAAAAGTTGGTTCCTGAACCAGATTacacaca                                                                                        >  2:1481940/1‑149 (MQ=255)
                                                              catGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCAca                                                                                        >  1:1514532/1‑149 (MQ=255)
                                                                tGCAGCACAACCAGGCTCGTGGCAGGCGTGAGGTTACCGTGGAAGATATTTACATCGGCAATGGGGTATCGGAGCTTCTCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCActct                                                                                      <  1:1143535/150‑1 (MQ=255)
                                                                  cagcaCTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTgg                                                                                    >  2:1763770/1‑150 (MQ=255)
                                                                  cagcaCTACCAGGCTCGTGGCATGCGTGATGTTACAGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTAACGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTgg                                                                                    >  2:1091732/1‑150 (MQ=255)
                                                                    gcaCGACCAGGCTCGTGGCATGAGTGATGTAACCGTGGAAGATATTTACAGCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGGGGGGACGAAATGTTGGTGCCTGCACGAGATTACCCACTCTGGAc                                                                                  >  1:1126198/1‑150 (MQ=255)
                                                                       cTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTTCACCAGATTACACACTCTGGACcgc                                                                               >  2:1205813/1‑150 (MQ=255)
                                                                          ccAGCCGCGTGGCACGCGTGCGGTTACCGTGGCAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCACTGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCGGCACCAGAGTACCCACTCTGGACCgcggc                                                                            <  2:291065/150‑1 (MQ=255)
                                                                            aGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGTCCGcggcgg                                                                          >  2:1230290/1‑150 (MQ=255)
                                                                                 cGTGGCATGCGTGATGTTACCGTGGAATATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGTCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCg                                                                     <  1:580206/150‑1 (MQ=255)
                                                                                 cGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCg                                                                     <  2:1333754/150‑1 (MQ=255)
                                                                                    ggCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCtt                                                                  <  2:1425576/150‑1 (MQ=255)
                                                                                     gCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCTTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCttt                                                                 <  2:1390241/150‑1 (MQ=255)
                                                                                            tGATGTTACCGTGGAAGATATTTACTTCGGCAATTGTGTATCGGAGCTTTTCGTTCAGGCAATGCAGTCATTGCTGAATAGCGGGGTCGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCTGCgg                                                          <  1:1317246/150‑1 (MQ=255)
                                                                                            tGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCgg                                                          <  1:1434696/150‑1 (MQ=255)
                                                                                                   aCCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCg                                                   >  2:215581/1‑150 (MQ=255)
                                                                                                    ccGTGGAAGATATTTCCCTCGGCAATGGTGTATCGGCCCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCTACTCTGGACCGCCGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                  <  2:1672341/150‑1 (MQ=255)
                                                                                                    ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                  >  2:1581028/1‑150 (MQ=255)
                                                                                                     cGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGt                                                 >  1:1592427/1‑150 (MQ=255)
                                                                                                      ggggAAGATATTTACATCGGCAATGGTGTAGCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTg                                                <  1:1413603/148‑1 (MQ=255)
                                                                                                      gTGGAAGATATTTACCTCGGCAATGGTGTATCGGAGCTTCTCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGGTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTg                                                <  2:1589947/150‑1 (MQ=255)
                                                                                                         gAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCAt                                             >  1:749135/1‑150 (MQ=255)
                                                                                                           aGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTa                                           <  1:706585/150‑1 (MQ=255)
                                                                                                           aGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTa                                           >  1:694814/1‑150 (MQ=255)
                                                                                                           aGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCAATGCTGAACAGCGCGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGCTAAAGAGGTGCATTa                                           >  2:1825869/1‑150 (MQ=255)
                                                                                                             atatTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATc                                         <  1:580928/150‑1 (MQ=255)
                                                                                                              tatTTACATCGTCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCt                                        <  2:1582920/150‑1 (MQ=255)
                                                                                                              tatTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCt                                        <  1:1410267/150‑1 (MQ=255)
                                                                                                               atTTACATCGGCAATGGTCTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCGGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCtt                                       <  1:204100/150‑1 (MQ=255)
                                                                                                                 ttACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTg                                     <  2:1855463/150‑1 (MQ=255)
                                                                                                                 ttACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTg                                     <  1:1427523/150‑1 (MQ=255)
                                                                                                                  tACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGc                                    >  2:205586/1‑150 (MQ=255)
                                                                                                                   aCATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGCAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCg                                   <  2:1065420/150‑1 (MQ=255)
                                                                                                                    cATCGGCTATGGTGTATCGGAGGTTTGCGTTCCGGCATTGCAGGCATTGCTGAACAGGGGGGAGGAAATGTTGGGTCCTGCACCAGATTTCCCAGTCTGGACCGCGGCGGTGTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGa                                  <  1:364886/150‑1 (MQ=255)
                                                                                                                    cATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTACCGCTGGAAAGCgaaac                                               >  2:482840/1‑133 (MQ=255)
                                                                                                                    cATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTACCGCTGGAAAGCgaaac                                               <  1:482840/137‑5 (MQ=255)
                                                                                                                     aTCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACTGCGGGGACGAAATGTGGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGAt                                 <  2:1195091/150‑1 (MQ=255)
                                                                                                                       cGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGa                               >  2:472201/1‑150 (MQ=255)
                                                                                                                       cGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGa                               <  1:1438264/150‑1 (MQ=255)
                                                                                                                           caTGGGGGATCGGAGCTTATCGTTCACGCAAAGCAGGCATTGCAGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATcc                           <  2:327243/149‑1 (MQ=255)
                                                                                                                             tGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCctc                         >  1:1830687/1‑150 (MQ=255)
                                                                                                                                tgtATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGATCAGCGGGGACGAAATGTTGGTTCCTGAACCAGATTACCCAATCTGGACCGCGGCGGTTTCGCTTGCGAGCGGTAAAGCGTTGCAGTATCTTTGCGAGGAATGCTCTGa                      >  1:1429121/1‑150 (MQ=255)
                                                                                                                                  tATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACt                    <  1:1060098/150‑1 (MQ=255)
                                                                                                                                   aTCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGACCAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTg                   >  1:890977/1‑150 (MQ=255)
                                                                                                                                   aTCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGATATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCATCTGACTg                   >  1:7078/1‑150 (MQ=255)
                                                                                                                                    tCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGATTgg                  <  1:1687827/150‑1 (MQ=255)
                                                                                                                                      ggAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGtt                >  2:518907/1‑150 (MQ=255)
                                                                                                                                       gAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGGTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTc               <  2:1026554/150‑1 (MQ=255)
                                                                                                                                          cTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGGTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCg            <  2:1505937/150‑1 (MQ=255)
                                                                                                                                            tATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGa          <  2:1277016/150‑1 (MQ=255)
                                                                                                                                             gctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGAc         >  2:1809100/4‑150 (MQ=255)
                                                                                                                                             gctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGAc         >  2:1801851/4‑150 (MQ=255)
                                                                                                                                             gctgTTCAGCAATGCCTGCATTGCCTGAAAAGCGGGGACGAAATGTTGGTTACTGCACCAGATTACCCACACTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATTAATACTCTGAATGGTTCCCGGAc         >  1:733255/4‑150 (MQ=255)
                                                                                                                                              ctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGAcc        >  1:1698849/3‑150 (MQ=255)
                                                                                                                                               cGTTCAGGCAATGCAGGCATTGCTGCACAGCGGGGACGAAATGTTGGTTCCGGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCGTGCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAAGCCTCTGCCTGGTTCCCGGACCt       >  1:1450142/1‑150 (MQ=255)
                                                                                                                                               cGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTCCCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCt       >  1:1679162/1‑150 (MQ=255)
                                                                                                                                               cGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCt       >  1:961788/1‑150 (MQ=255)
                                                                                                                                                gTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTc      >  1:256955/1‑150 (MQ=255)
                                                                                                                                                gTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGAAGAAATGATGGTTCCTGAACCCGATTAACCACTCTGGACCGCGGCGGTTTCGCTTTCAAGCGTTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTc      >  2:72260/1‑150 (MQ=255)
                                                                                                                                                 ttCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAATTGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCg     <  2:226104/150‑1 (MQ=255)
                                                                                                                                                    aGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGAGCTCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCTTTATGTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCgatg  >  2:1243021/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACCTGCCTACCGCTCAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATG  >  NC_000913/2407741‑2408038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: