Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,609,561:1 +C 5.2% coding (1120/1452 nt) uxaB ← altronate oxidoreductase, NAD‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,609,5611.C 5.2% 483.5 / 21.8 115coding (1120/1452 nt)uxaBaltronate oxidoreductase, NAD‑dependent
Reads supporting (aligned to +/- strand):  ref base . (62/47);  new base C (4/2);  total (66/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.53e-01

Only 100 of 118 total aligned reads displayed.

TGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTT  >  NC_000913/1609413‑1609710
                                                                                                                                                     |                                                                                                                                                     
tGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtgt                                                                                                                                                      <  1:1790891/150‑1 (MQ=255)
 gCTGAAAACGGACCAGCCAGTGTGCATCGTCCGGTACCGTATATGCTTCGCCGTTACGCTCACCACGATAGAACGCAATCAAAGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCtgtg                                                                                                                                                     <  2:350941/150‑1 (MQ=255)
  cTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTgg                                                                                                                                                    >  1:758295/1‑150 (MQ=255)
  cTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTgg                                                                                                                                                    <  1:1554678/150‑1 (MQ=255)
  cTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTgg                                                                                                                                                    >  2:796626/1‑150 (MQ=255)
   ggATACCGTTCCAGCCCGTGTGCATCGTCCTGTACCGGATATGGTTCGCCGTTACGCTCACCACGATAGACCGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGc                                                                                                                                                   <  1:335162/149‑1 (MQ=255)
    gATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGTCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCa                                                                                                                                                  <  2:925039/150‑1 (MQ=255)
    gATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCa                                                                                                                                                  >  1:856852/1‑150 (MQ=255)
         cGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCACAGCTGCTGGCAgggca                                                                                                                                             <  1:1875539/150‑4 (MQ=255)
             ccagccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCggg                                                                                                                                         <  1:505900/150‑1 (MQ=255)
             ccagccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCggg                                                                                                                                         >  1:1670400/1‑150 (MQ=255)
               agccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCATGATGCGGGTg                                                                                                                                       >  2:1676951/1‑150 (MQ=255)
                gccagTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTGACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGc                                                                                                                                      <  1:1291768/150‑1 (MQ=255)
                       tgCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACtt                                                                                                                               >  1:1790918/1‑150 (MQ=255)
                        gCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTg                                                                                                                              >  2:15826/1‑150 (MQ=255)
                         cATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTgg                                                                                                                             >  2:1591295/1‑150 (MQ=255)
                          ctCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGt                                                                                                                            <  2:437566/149‑1 (MQ=255)
                           tCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTc                                                                                                                           <  1:1671756/150‑1 (MQ=255)
                           tCGTCCTGTACCGGATATGTTTCGCCGTAACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTTCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTc                                                                                                                           <  1:727364/150‑1 (MQ=255)
                            cGTCCTGTACCGGATATGATTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCa                                                                                                                          <  2:632514/150‑1 (MQ=255)
                             gTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCAt                                                                                                                         >  1:662180/1‑150 (MQ=255)
                                cTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATAcc                                                                                                                      >  2:213948/1‑150 (MQ=255)
                                cTGTACCGGATATGTTTCGCCGTTACGCCCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGGCTTCTGCCCTGCCGGCAGCTGTGCCAGGATGCGGGTGCGGAACTTGGTCAAAcc                                                                                                                      >  2:751927/1‑150 (MQ=255)
                                cTGTACCGGATATGTTTCGCCGGTACGCTCACCACGATAGAACGCAATCAATGCCGCTCATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATAcc                                                                                                                      <  1:1132991/150‑1 (MQ=255)
                                cTGTACCGGATAAGTTTCGCCGTTACGCGCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATAcc                                                                                                                      <  1:301216/150‑1 (MQ=255)
                                cTGGACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCACTGCCGCTAATGCGACAGTACGGCGCGCCGGAAGGGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATAcc                                                                                                                      <  2:20410/150‑1 (MQ=255)
                                  gTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATGCCGt                                                                                                                    >  2:652976/1‑150 (MQ=255)
                                   tACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGtt                                                                                                                   >  2:977325/1‑150 (MQ=255)
                                    aCCGGATATGTTTCGCCGTTCCGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTg                                                                                                                  <  2:393998/150‑1 (MQ=255)
                                    aCCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTg                                                                                                                  >  2:553392/1‑150 (MQ=255)
                                      cGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAg                                                                                                                >  1:1729288/1‑150 (MQ=255)
                                      cGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAg                                                                                                                >  1:1430380/1‑150 (MQ=255)
                                      cGGATATGATTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCCGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAg                                                                                                                >  2:695081/1‑150 (MQ=255)
                                       ggATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAgc                                                                                                               >  1:1860501/1‑150 (MQ=255)
                                          tatGTTTCGCCGTTACGCTCACCACGATAGAACGCACTCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTCCCTGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGcgcg                                                                                                            >  1:82586/1‑150 (MQ=255)
                                              tttCGCCGGTACGCGCACCACGATAGCACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAg                                                                                                        <  2:316448/150‑1 (MQ=255)
                                               ttCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTTCGGAACTTGGTCATACCGTTGAGCGCGATAGa                                                                                                       >  1:519762/1‑150 (MQ=255)
                                               ttCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGa                                                                                                       >  1:1060599/1‑150 (MQ=255)
                                                  gCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcag                                                                                                    >  1:428851/1‑150 (MQ=255)
                                                  gCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcag                                                                                                    <  2:1355232/150‑1 (MQ=255)
                                                    cGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCCGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagca                                                                                                  >  1:1430612/1‑150 (MQ=255)
                                                     gTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGAcagcag                                                                                                 <  2:1860501/150‑1 (MQ=255)
                                                       tACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCt                                                                                               >  2:111102/1‑150 (MQ=255)
                                                         cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGa                                                                                             >  2:653644/1‑150 (MQ=255)
                                                         cGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGa                                                                                             <  1:1578721/150‑1 (MQ=255)
                                                           cTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATg                                                                                           >  2:753475/1‑150 (MQ=255)
                                                                 acGATATAACGCAAGCAATGCCGATAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAAt                                                                                     <  1:892363/150‑1 (MQ=255)
                                                                 acGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAAt                                                                                     >  1:207754/1‑150 (MQ=255)
                                                                 acGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGGGCGGAACGTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAAt                                                                                     >  1:315386/1‑150 (MQ=255)
                                                                  cGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATg                                                                                    <  1:896228/150‑1 (MQ=255)
                                                                       gaaaccaaacaaaGCCGCTAATCCGAAAGTAAGGAGCCCCTGAAGTGAGCCGTTTTACTTATGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACgg                                                                               >  2:1762172/6‑150 (MQ=255)
                                                                            caatcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTAc                                                                          <  2:622209/150‑1 (MQ=255)
                                                                             aatcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCTATAGACAGCAGCTGATGCNTAATGTACGGGTTACg                                                                         <  1:1517877/150‑1 (MQ=255)
                                                                             aatcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACg                                                                         >  2:1467693/1‑150 (MQ=255)
                                                                             aatcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACg                                                                         <  2:53085/150‑1 (MQ=255)
                                                                               tcaatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGa                                                                       <  2:593679/150‑1 (MQ=255)
                                                                                 aatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGCCCGCAGCTGATGCTTAATGTACGGGTTACGGaaa                                                                     >  2:899486/1‑150 (MQ=255)
                                                                                 aatGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGaaa                                                                     <  2:1722553/150‑1 (MQ=255)
                                                                                  atGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAAc                                                                    <  1:472436/150‑1 (MQ=255)
                                                                                     ccGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGcc                                                                 <  1:373134/150‑1 (MQ=255)
                                                                                            tGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTgcc                                                          >  1:1396672/1‑148 (MQ=255)
                                                                                            tGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAc                                                          >  1:1175625/1‑150 (MQ=255)
                                                                                            tGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAc                                                          >  2:290396/1‑150 (MQ=255)
                                                                                            tGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAc                                                          >  1:483462/1‑150 (MQ=255)
                                                                                            tGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGAc                                                          >  2:1846580/1‑150 (MQ=255)
                                                                                             gCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGGGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTCAGGAATCGCCCGGTGTCt                                                         >  2:220772/1‑150 (MQ=255)
                                                                                             gCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACt                                                         >  2:859287/1‑150 (MQ=255)
                                                                                              cGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTg                                                        >  1:1059929/1‑150 (MQ=255)
                                                                                              cGAAAGGAACGCGCGCCGGACGTGGCCCGTTTGCCCTCTGCCCTGCCAGCCGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCTTACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTGACGGAAACGCCCGGTGACTg                                                        <  2:297053/150‑1 (MQ=255)
                                                                                                  aGTAAGGCGCGCCGGAAGTGGGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACt                                                    <  1:1124090/150‑1 (MQ=255)
                                                                                                     aaGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGAAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTGGAGCGCGATAGACAGCAGCTGATGCTTAATGGACGGGTTACGGAATCGCCCGTTGACTGCACTGGc                                                 >  1:274619/1‑150 (MQ=255)
                                                                                                     aaGGCGCGCCGGAAGGGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGc                                                 <  2:1080380/150‑1 (MQ=255)
                                                                                                       ggCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGCACTTGGTCATACCGTTGAGCGCGATAGACAGCACCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGa                                               >  2:165912/1‑150 (MQ=255)
                                                                                                       ggCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTAAGGGTTACGGAAACGCCCGGTGACTGCACTGGCGa                                               >  2:608995/1‑150 (MQ=255)
                                                                                                         cgcgcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGaaa                                             >  1:418574/1‑150 (MQ=255)
                                                                                                         cgcgcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGaaa                                             <  1:486434/150‑1 (MQ=255)
                                                                                                          gcgcCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAg                                            <  1:1311481/150‑1 (MQ=255)
                                                                                                             ccGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGAtt                                         >  1:614437/1‑150 (MQ=255)
                                                                                                               ggAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTcc                                       <  1:1694435/150‑1 (MQ=255)
                                                                                                                  aGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTTAGCTCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGt                                    <  1:1699915/150‑1 (MQ=255)
                                                                                                                      ggCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTtcat                                <  1:598593/149‑1 (MQ=255)
                                                                                                                       gCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTtcatc                               >  2:72079/1‑150 (MQ=255)
                                                                                                                       gCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTtcatc                               >  2:503355/1‑150 (MQ=255)
                                                                                                                        ccGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTtcatca                              <  2:428851/150‑1 (MQ=255)
                                                                                                                            ttGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAg                          <  1:652976/150‑1 (MQ=255)
                                                                                                                                cccgcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCaa                      >  1:1763403/8‑150 (MQ=255)
                                                                                                                                cccgcatcctgccACAGCTGCTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCaa                      >  1:1272665/8‑150 (MQ=255)
                                                                                                                                    tgccctgccAGCAGCTGTGGCAGGATGCGGGGGCGGAACTTGGTCATACCGTTCAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGGTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATcc                  <  2:964350/150‑1 (MQ=255)
                                                                                                                                      ccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAg                <  2:758295/150‑1 (MQ=255)
                                                                                                                                      ccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAg                >  1:1046787/1‑150 (MQ=255)
                                                                                                                                      ccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAg                <  1:796626/150‑1 (MQ=255)
                                                                                                                                      ccctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAg                >  1:204779/1‑150 (MQ=255)
                                                                                                                                      ccctgccAGCAGCTGGGGCATGATGCGGGTGCGGACCTTGTTCATACCGTTGAGCGCGATAGACAGCAGATGATGATTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAg                <  2:519762/150‑1 (MQ=255)
                                                                                                                                       cctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGt               >  1:621087/1‑150 (MQ=255)
                                                                                                                                        ctgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGCTTCCAGTTCATCACGAGGCAAATCCAGTa              >  2:1104883/1‑150 (MQ=255)
                                                                                                                                         tgccAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTAc             <  1:987039/150‑1 (MQ=255)
                                                                                                                                            cagcagCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCgg          >  2:484469/1‑150 (MQ=255)
                                                                                                                                                 gcTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGaataa     <  1:590367/150‑1 (MQ=255)
                                                                                                                                                 gcTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGaataa     >  2:1824013/1‑150 (MQ=255)
                                                                                                                                                    gtgGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGACCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAAttt  >  2:131466/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCTGATAACGTTCCAGCCAGTGTGCATCGTCCTGTACCGGATATGTTTCGCCGTTACGCTCACCACGATAGAACGCAATCAATGCCGCTAATGCGAAAGTAAGGCGCGCCGGAAGTGTGCCGTTTGCCTTCTGCCCTGCCAGCAGCTGTGGCAGGATGCGGGTGCGGAACTTGGTCATACCGTTGAGCGCGATAGACAGCAGCTGATGCTTAATGTACGGGTTACGGAAACGCCCGGTGACTGCACTGGCGAAAGATTCCAGTTCATCACGAGGCAAATCCAGTACCGGAATAATTT  >  NC_000913/1609413‑1609710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: