Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,407,905:1 +C 6.1% coding (345/1218 nt) alaA → glutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,407,9051.C 6.1% 544.1 / 31.5 132coding (345/1218 nt)alaAglutamate‑pyruvate aminotransferase; glutamic‑pyruvic transaminase (GPT); alanine transaminase
Reads supporting (aligned to +/- strand):  ref base . (67/57);  new base C (6/2);  total (73/59)
Fisher's exact test for biased strand distribution p-value = 2.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01

Only 100 of 134 total aligned reads displayed.

CAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAAT  >  NC_000913/2407756‑2408054
                                                                                                                                                      |                                                                                                                                                     
cAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTg                                                                                                                                                        >  1:226104/1‑150 (MQ=255)
 acgcgTATTGCGAATCCAAAGGTCTTTCCTCCGCGCGTATAGCCATCATCCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACCTCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGc                                                                                                                                                      <  2:120595/146‑1 (MQ=255)
  aGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGATCAGGCAATGCAGGCATTGCt                                                                                                                                                     >  1:1731362/1‑150 (MQ=255)
   gggTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATGATGAAGCACTACCAGGCTCGGGGCATGCGTGATGTTACCGAGGAAGATATTTACATCGGCAATGGTGTAGCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTg                                                                                                                                                    <  1:160387/150‑1 (MQ=255)
      tATTGCGATTCCAAAGGCCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAAc                                                                                                                                                 <  1:781506/150‑1 (MQ=255)
      tATTGCGAATCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGCAATGCCTGCATTGCCTGAAc                                                                                                                                                 <  1:1144021/150‑1 (MQ=255)
      aaatGCGAGTCCAAAGGTCTCTACGCCGCGCGTAACGCCATCCTGCAGCACTACCAGGCTCGTGGCAGGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGCAATGCCTGCATTGCCTGAAc                                                                                                                                                 <  2:907814/147‑1 (MQ=255)
          gCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCg                                                                                                                                             >  2:35800/1‑150 (MQ=255)
           cGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACGGTGGAAGATATTTTCATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCgg                                                                                                                                            >  2:231310/1‑150 (MQ=255)
               tCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGAc                                                                                                                                        >  1:10182/1‑150 (MQ=255)
                ccAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACg                                                                                                                                       >  2:883323/1‑150 (MQ=255)
                       tCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGtt                                                                                                                                <  1:254229/150‑1 (MQ=255)
                        cTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTg                                                                                                                               <  1:1664568/150‑1 (MQ=255)
                         tttACTCCGCGCGTAAAGACATCATGCAGGACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTgg                                                                                                                              <  2:1103283/150‑1 (MQ=255)
                           tACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGATACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGtt                                                                                                                            <  1:1190387/150‑1 (MQ=255)
                             cTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTAGCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCGGAACAGCGGGGACGAAATGTTGGTTcc                                                                                                                          <  2:261294/150‑1 (MQ=255)
                             cTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGGGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTcc                                                                                                                          <  1:1323177/150‑1 (MQ=255)
                             cTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGCGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTcc                                                                                                                          >  1:1364389/1‑150 (MQ=255)
                               ccGCGCGTAAAGCCATCATGCAGCACTCCCAGGCTCGTGGCATGCGTGATGTTACCGGGGAAGCTTTTTAGATCGGCAATGGGGTATCTGAGCTTATCGTTCAGGCATTGCAGGCTTTGCTGAACAGCGGGGCCGCATTGTGGGTTCCTg                                                                                                                        >  2:946864/1‑150 (MQ=255)
                               ccGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCATGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTg                                                                                                                        >  2:958279/1‑150 (MQ=255)
                               ccGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTg                                                                                                                        <  2:1489065/150‑1 (MQ=255)
                               ccGAGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGATGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTg                                                                                                                        >  2:193453/1‑150 (MQ=255)
                                cgcgcgTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATACTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTTGTTCCTGc                                                                                                                       >  2:1208135/1‑150 (MQ=255)
                                cgcgcgTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATACTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGc                                                                                                                       >  2:443201/1‑150 (MQ=255)
                                 gcgcgTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTAACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGGGCTTATCGTTCACGCAATGCACTCATTGCTGAACAGCCGGGCCGAAATCTTGGTTCCTGcc                                                                                                                      >  1:118622/1‑149 (MQ=255)
                                  cgagTAAAGCCAGCATGCAGCACTACCAGTCTCGGGGCATGCGGGATGTTACCGTTGAATATATTTACATCGTCAATGTTGTATAGGAGATTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCAc                                                                                                                     <  2:563526/147‑1 (MQ=255)
                                    cgTAAAGCCATCATGGAGCACTAGCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGGATCGGAGCGTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGGACCa                                                                                                                   <  1:984472/150‑1 (MQ=255)
                                    cgTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCa                                                                                                                   >  1:327243/1‑150 (MQ=255)
                                    cgTAAAGCCAACATGCAGCACAACCAGGCTCGTGGCATGCGTGATGTTACCGTGCAACATAAAAACATCGGCAATGCTGTATCCCAGCTTACCGTTCACGCAATCCAGGCATAGCTGAACAGCGGGGACGAAATGTTGGTTCCAGCACCa                                                                                                                   <  1:990757/150‑1 (MQ=255)
                                     gTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAg                                                                                                                  <  2:878699/150‑1 (MQ=255)
                                       aaaGCCAGCATGCAGCACTACCAGGCTCGTGGCTTGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGAt                                                                                                                <  1:320895/150‑1 (MQ=255)
                                         aGCCATAATGCAGCACTACCAGGCTCGTGGCTTGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGAGTa                                                                                                              >  2:1634540/1‑150 (MQ=255)
                                          gCCAGCATGCAGCACTACCAGGCTCGTGTCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCGTATCGTTCAGGCAATGCAGGCAGTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTAc                                                                                                             <  1:291088/150‑1 (MQ=255)
                                           ccATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGACCAGCGGGGACGAATTGTTGGTTCCTGCTCCAGATTAcc                                                                                                            >  1:145634/1‑150 (MQ=255)
                                            catcatGCAGCACTACCAGGCTCGTGGCTTGCGTGATGTTACCGTGGAAGATAATTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGATCCTGCACCAGATTAccc                                                                                                           >  1:1341138/1‑150 (MQ=255)
                                               catGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATTGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATAGATGAACAGCGGGAACGAAAAGTTGGTTCCTGAACCAGATTacacaca                                                                                                        >  2:1481940/1‑149 (MQ=255)
                                               catGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCAca                                                                                                        >  1:1514532/1‑149 (MQ=255)
                                                 tGCAGCACAACCAGGCTCGTGGCAGGCGTGAGGTTACCGTGGAAGATATTTACATCGGCAATGGGGTATCGGAGCTTCTCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCActct                                                                                                      <  1:1143535/150‑1 (MQ=255)
                                                   cagcaCTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTgg                                                                                                    >  2:1763770/1‑150 (MQ=255)
                                                   cagcaCTACCAGGCTCGTGGCATGCGTGATGTTACAGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTAACGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTgg                                                                                                    >  2:1091732/1‑150 (MQ=255)
                                                    agcaCTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGa                                                                                                   >  1:1412466/1‑150 (MQ=255)
                                                     gcaCGACCAGGCTCGTGGCATGAGTGATGTAACCGTGGAAGATATTTACAGCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGGGGGGACGAAATGTTGGTGCCTGCACGAGATTACCCACTCTGGAc                                                                                                  >  1:1126198/1‑150 (MQ=255)
                                                        cTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTTCACCAGATTACACACTCTGGACcgc                                                                                               >  2:1205813/1‑150 (MQ=255)
                                                           ccAGCCGCGTGGCACGCGTGCGGTTACCGTGGCAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCACTGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCGGCACCAGAGTACCCACTCTGGACCgcggc                                                                                            <  2:291065/150‑1 (MQ=255)
                                                                  cGTGGCATGCGTGATGTTACCGTGGAATATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGTCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCg                                                                                     <  1:580206/150‑1 (MQ=255)
                                                                     ggCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCtt                                                                                  <  2:1425576/150‑1 (MQ=255)
                                                                      gCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCTTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCttt                                                                                 <  2:1390241/150‑1 (MQ=255)
                                                                       cATGCGTGATGTTACCGTGGAAGATATTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCCGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTcc                                                                               <  1:1104427/150‑1 (MQ=255)
                                                                             tGATGTTACCGTGGAAGATATTTACTTCGGCAATTGTGTATCGGAGCTTTTCGTTCAGGCAATGCAGTCATTGCTGAATAGCGGGGTCGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCTGCgg                                                                          <  1:1317246/150‑1 (MQ=255)
                                                                             tGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTGAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCgg                                                                          <  1:1432658/150‑1 (MQ=255)
                                                                                  ttACCGTGGAAGATATTTACCTCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGCGACGAAATGTTGGTTCCTGCACCAGATTACCAACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAg                                                                     >  1:435486/1‑150 (MQ=255)
                                                                                  ttACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCCCCCGATTACCCCCTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAg                                                                     >  2:137352/1‑150 (MQ=255)
                                                                                    aCCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCg                                                                   >  2:215581/1‑150 (MQ=255)
                                                                                     ccGTGGAAGATATTTCCCTCGGCAATGGTGTATCGGCCCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCTACTCTGGACCGCCGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                                  <  2:1672341/150‑1 (MQ=255)
                                                                                     ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTTTCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCATGCACCAGATTACAAACTCTGGAACGCGGCTGTTTCGCTTTACAGCGGTAAAGCgg                                                                  >  1:927584/1‑150 (MQ=255)
                                                                                     ccGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCgg                                                                  >  2:1581028/1‑150 (MQ=255)
                                                                                      cGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGt                                                                 >  1:1592427/1‑150 (MQ=255)
                                                                                       gTGGAAGATATTTACCTCGGCAATGGTGTATCGGAGCTTCTCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGGTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTg                                                                <  2:1589947/150‑1 (MQ=255)
                                                                                          gAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCAt                                                             >  1:749135/1‑150 (MQ=255)
                                                                                            aGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTa                                                           <  1:706585/150‑1 (MQ=255)
                                                                                            aGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTa                                                           >  1:694814/1‑150 (MQ=255)
                                                                                            aGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCAATGCTGAACAGCGCGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGCTAAAGAGGTGCATTa                                                           >  2:1825869/1‑150 (MQ=255)
                                                                                              atatTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATc                                                         <  1:580928/150‑1 (MQ=255)
                                                                                               tatTTACATCGTCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCt                                                        <  2:1582920/150‑1 (MQ=255)
                                                                                               tatTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCt                                                        <  1:1410267/150‑1 (MQ=255)
                                                                                                atTTACATCGGCAATGGTCTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCGGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCtt                                                       <  1:204100/150‑1 (MQ=255)
                                                                                                  ttACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTg                                                     <  1:1427523/150‑1 (MQ=255)
                                                                                                  ttACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTg                                                     <  2:1855463/150‑1 (MQ=255)
                                                                                                   tACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGc                                                    >  2:1285849/1‑150 (MQ=255)
                                                                                                   tACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGc                                                    >  2:205586/1‑150 (MQ=255)
                                                                                                    aCATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGCAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCg                                                   <  2:1065420/150‑1 (MQ=255)
                                                                                                     cATCGGCTATGGTGTATCGGAGGTTTGCGTTCCGGCATTGCAGGCATTGCTGAACAGGGGGGAGGAAATGTTGGGTCCTGCACCAGATTTCCCAGTCTGGACCGCGGCGGTGTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGa                                                  <  1:364886/150‑1 (MQ=255)
                                                                                                     cATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTACCGCTGGAAAGCgaaac                                                               >  2:482840/1‑133 (MQ=255)
                                                                                                        cGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGa                                               <  1:1438264/150‑1 (MQ=255)
                                                                                                        cGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGa                                               >  2:472201/1‑150 (MQ=255)
                                                                                                                 tgtATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGATCAGCGGGGACGAAATGTTGGTTCCTGAACCAGATTACCCAATCTGGACCGCGGCGGTTTCGCTTGCGAGCGGTAAAGCGTTGCAGTATCTTTGCGAGGAATGCTCTGa                                      >  1:1429121/1‑150 (MQ=255)
                                                                                                                   tATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACt                                    <  1:1060098/150‑1 (MQ=255)
                                                                                                                    aTCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGACCAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTg                                   >  1:890977/1‑150 (MQ=255)
                                                                                                                    aTCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGATATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCATCTGACTg                                   >  1:7078/1‑150 (MQ=255)
                                                                                                                    aTCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTg                                   >  2:336774/1‑150 (MQ=255)
                                                                                                                     tCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGATTgg                                  <  1:1687827/150‑1 (MQ=255)
                                                                                                                       ggAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGtt                                >  2:518907/1‑150 (MQ=255)
                                                                                                                           cTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGGTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCg                            <  2:1505937/150‑1 (MQ=255)
                                                                                                                             tATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGa                          <  2:1277016/150‑1 (MQ=255)
                                                                                                                              gctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGAc                         >  2:1809100/4‑150 (MQ=255)
                                                                                                                              gctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGAc                         >  2:1801851/4‑150 (MQ=255)
                                                                                                                              gctgTTCAGCAATGCCTGCATTGCCTGAAAAGCGGGGACGAAATGTTGGTTACTGCACCAGATTACCCACACTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATTAATACTCTGAATGGTTCCCGGAc                         >  1:733255/4‑150 (MQ=255)
                                                                                                                               ctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGAcc                        >  1:1698849/3‑150 (MQ=255)
                                                                                                                               ctgTTCAGCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGCTTCCCGGAcc                        >  1:1691236/3‑150 (MQ=255)
                                                                                                                                cGTTCAGGCAATGCAGGCATTGCTGCACAGCGGGGACGAAATGTTGGTTCCGGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCGTGCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAAGCCTCTGCCTGGTTCCCGGACCt                       >  1:1450142/1‑150 (MQ=255)
                                                                                                                                cGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTCCCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCt                       >  1:1679162/1‑150 (MQ=255)
                                                                                                                                cGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCt                       >  1:961788/1‑150 (MQ=255)
                                                                                                                                 gTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGAAGAAATGATGGTTCCTGAACCCGATTAACCACTCTGGACCGCGGCGGTTTCGCTTTCAAGCGTTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTc                      >  2:72260/1‑150 (MQ=255)
                                                                                                                                    tcagCAATGCCTGCATTGCCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCga                    >  1:1230361/4‑150 (MQ=255)
                                                                                                                                    tcagCAATGCCTGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCgat                   >  1:620191/4‑150 (MQ=255)
                                                                                                                                    cAGGCAATTCCGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCgat                   <  2:85052/150‑1 (MQ=255)
                                                                                                                                     cagCAATGCCTGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATAACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGGAACGCGGGCCATTATCTTTGCGATGAATCCTCGGACTGGTTCCCGGACCTCgagg                  >  2:1843230/3‑148 (MQ=255)
                                                                                                                                     aGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGAGCTCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCTTTATGTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCgatg                  >  2:1243021/1‑150 (MQ=255)
                                                                                                                                           tGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCTCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTc            >  2:1666508/1‑150 (MQ=255)
                                                                                                                                               ggCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCgcgc        <  1:35800/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CAAGGGTATTGCGATTCCAAAGGTCTTTACTCCGCGCGTAAAGCCATCATGCAGCACTACCAGGCTCGTGGCATGCGTGATGTTACCGTGGAAGATATTTACATCGGCAATGGTGTATCGGAGCTTATCGTTCAGGCAATGCAGGCATTGCTGAACAGCGGGGACGAAATGTTGGTTCCTGCACCAGATTACCCACTCTGGACCGCGGCGGTTTCGCTTTCCAGCGGTAAAGCGGTGCATTATCTTTGCGATGAATCCTCTGACTGGTTCCCGGACCTCGATGATATTCGCGCTAAAAT  >  NC_000913/2407756‑2408054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: