Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,127,799 C→A 5.0% intergenic (+70/‑40) yceM → / → murJ putative oxidoreductase/putative lipid II flippase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,127,7990CA 5.0% 326.6 / 4.7 108intergenic (+70/‑40)yceM/murJputative oxidoreductase/putative lipid II flippase
Reads supporting (aligned to +/- strand):  ref base C (37/65);  new base A (3/3);  total (40/68)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

TCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGA  >  NC_000913/1127651‑1127944
                                                                                                                                                    |                                                                                                                                                 
   gCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGc                                                                                                                                               >  2:1709694/1‑150 (MQ=255)
       aCCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTTGAGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCTCCTCTTTCAGCGCCTGCCTTGCAGGc                                                                                                                                           >  1:1214351/1‑150 (MQ=255)
         cgccgGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGt                                                                                                                                         >  1:1025347/1‑150 (MQ=255)
          gccgGCGACCAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAACCCCTGTAACATCTGGCGGTAGCAATTCACCATCATCCAGGTAGACTATTCGCCTCTATCAGCGCCTGCCATGCAGGCGtt                                                                                                                                        <  2:1465324/150‑1 (MQ=255)
          gccgGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCAAGGCAGGCgct                                                                                                                                        <  1:837875/150‑3 (MQ=255)
          gccgGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCAAGGCAGGCgct                                                                                                                                        <  1:845228/150‑3 (MQ=255)
           ccgGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGttt                                                                                                                                       >  2:387194/1‑150 (MQ=255)
            cgGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGtttt                                                                                                                                      <  1:967340/150‑1 (MQ=255)
             ggCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTg                                                                                                                                     <  1:960644/150‑1 (MQ=255)
             ggCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCAAGGCAGGCgctgaa                                                                                                                                     <  1:507653/150‑6 (MQ=255)
             ggCGAACAGGCCGTGCTGGCGCAACGTATCGGTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATAAGCCTCTTTCAGCGCCTGCCTTGCAGGCGTGTTg                                                                                                                                     >  1:320883/1‑150 (MQ=255)
              gCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGc                                                                                                                                    <  1:2673/150‑1 (MQ=255)
               cGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGcc                                                                                                                                   >  1:1373327/1‑150 (MQ=255)
                     gtccGTGCTTGCGAAACGTATCGTTGACAAGTTGTGGCGCGATGCGATGAGTGAATAAACCCTGTTAAATATGGCGGTAGCAATTCACCATAATCCAGGTAGACTTTTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTggg                                                                                                                             <  1:1074604/148‑1 (MQ=255)
                     ggCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAACCCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCAGTggg                                                                                                                             >  2:1263326/1‑150 (MQ=255)
                     ggCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTggg                                                                                                                             <  1:1604097/150‑1 (MQ=255)
                     ggCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTggg                                                                                                                             >  1:536404/1‑150 (MQ=255)
                      gCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGt                                                                                                                            <  2:125151/150‑1 (MQ=255)
                         gTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTg                                                                                                                         <  2:900022/150‑1 (MQ=255)
                          ggCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTgg                                                                                                                        <  2:1771636/149‑1 (MQ=255)
                            cTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGAt                                                                                                                      <  1:1504052/150‑1 (MQ=255)
                            cTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGAAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGAt                                                                                                                      <  2:71626/150‑1 (MQ=255)
                             tGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCCCCATAATCAAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCGTGCAGGCGGTGTGCCCGTGGGTCTGGAtt                                                                                                                     >  2:221380/1‑150 (MQ=255)
                             tGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGAtt                                                                                                                     <  2:1278142/150‑1 (MQ=255)
                             tGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGAtt                                                                                                                     <  2:602730/150‑1 (MQ=255)
                             tGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGAtt                                                                                                                     <  2:957134/150‑1 (MQ=255)
                             gggCGCAACGTATCGTGGACAAGATCTGGCGCGATGCGATGAGGGAATACACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGTCTTGCAGGCGTTTTGCCCGTGGGGCTGGAtt                                                                                                                     <  2:880924/149‑1 (MQ=255)
                               gcgcAACGGATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAg                                                                                                                   <  1:1684282/150‑1 (MQ=255)
                                cgcAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTGTTGCCCGTGGGTCTGGATTAGa                                                                                                                  >  2:1745027/1‑150 (MQ=255)
                                 gcAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGaa                                                                                                                 <  1:1867870/150‑1 (MQ=255)
                                 gcAACGTAGCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGaa                                                                                                                 <  2:811704/150‑1 (MQ=255)
                                  cAACGGATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCCTAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGCTTAGAac                                                                                                                >  2:1247050/1‑150 (MQ=255)
                                      gTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCg                                                                                                            >  1:1748147/1‑150 (MQ=255)
                                       tATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGa                                                                                                           <  2:419724/150‑1 (MQ=255)
                                         tCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATg                                                                                                         <  1:1408219/150‑1 (MQ=255)
                                         tCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATg                                                                                                         <  2:1425366/150‑1 (MQ=255)
                                           gTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGaa                                                                                                       >  2:1238011/1‑150 (MQ=255)
                                               aCAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATtta                                                                                                   <  2:1033122/150‑1 (MQ=255)
                                               aCAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGAAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATtta                                                                                                   <  2:195733/150‑1 (MQ=255)
                                                cAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATttat                                                                                                  >  2:442796/1‑150 (MQ=255)
                                                cAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATttat                                                                                                  <  2:1565066/150‑1 (MQ=255)
                                                cAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATttat                                                                                                  <  2:864305/150‑1 (MQ=255)
                                                 aaGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAAtttttt                                                                                                 <  1:799819/150‑1 (MQ=255)
                                                     tCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTaaaa                                                                                             <  1:544458/150‑1 (MQ=255)
                                                          cgcgATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCt                                                                                        <  1:887139/150‑1 (MQ=255)
                                                           gcgatgcgatGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCTCTg                                                                                       <  1:506486/150‑1 (MQ=255)
                                                            cgatgcgatgAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTCTTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTgg                                                                                      <  2:1510401/150‑1 (MQ=255)
                                                            cgatgcgatgAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTgg                                                                                      <  1:962398/150‑1 (MQ=255)
                                                            cgaagcgatgAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCGTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAAACGCTgg                                                                                      >  2:251969/1‑150 (MQ=255)
                                                                 cgatgAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGgccgcc                                                                                 <  2:239733/150‑1 (MQ=255)
                                                                 cgatgAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCACGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGgccgcc                                                                                 >  1:1833231/1‑150 (MQ=255)
                                                                  gattAGTGAATAAACCCTGTAACATCTGGCGGTAGCAAGTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTGGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGccgccg                                                                                <  2:711529/150‑1 (MQ=255)
                                                                      aGTGAATAAACCCTGTAACATCTTGCGGTATCAATTCACCAAAAACCAGGTAGACTATTAGCCTGTTTCAGCGCCTGCATTGCAGGCGTTTTGCCAGTGGGTCTGGATTAGAACACAGATCAATTTATTAACATAGCTGgacgcattcac                                                                            >  2:724162/1‑144 (MQ=255)
                                                                      aGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAg                                                                            >  2:1449457/1‑150 (MQ=255)
                                                                          aaTAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCg                                                                        >  2:1802437/1‑150 (MQ=255)
                                                                          aaTAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCg                                                                        <  1:841358/150‑1 (MQ=255)
                                                                           aTAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGa                                                                       >  1:1841072/1‑150 (MQ=255)
                                                                             aaaCCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATg                                                                     <  1:1465564/150‑1 (MQ=255)
                                                                               aCCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGAc                                                                   <  2:885865/150‑1 (MQ=255)
                                                                                 ccTGTATCATCTGGCGGTAGCAATTCACCATAATCCAGGTAGTCTTTTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCa                                                                 <  1:209079/150‑1 (MQ=255)
                                                                                    gTAACATCTGGCGGTAGCAATTCACCATAATCCAGTTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACAACGAAGAATTTATTAAAATCGATGGCCGCGGTCAGCTCGATGACCATGt                                                              >  1:804067/1‑150 (MQ=255)
                                                                                        cATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTc                                                          >  2:1718575/1‑150 (MQ=255)
                                                                                           cTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTcgcg                                                       >  2:1521055/1‑150 (MQ=255)
                                                                                            tGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCgt                                                      <  1:1162197/150‑1 (MQ=255)
                                                                                              gCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCgtgt                                                    <  1:132191/150‑1 (MQ=255)
                                                                                                           aCCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCa                                       <  1:1809926/150‑1 (MQ=255)
                                                                                                            ccATAATCCAGGTAGCCTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCAc                                      >  2:1466518/1‑150 (MQ=255)
                                                                                                            ccATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCAc                                      <  1:198773/150‑1 (MQ=255)
                                                                                                               tAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACgag                                   <  2:1207171/150‑1 (MQ=255)
                                                                                                                 aTCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTCGCTTCGCACgagcc                                 >  2:391785/1‑148 (MQ=255)
                                                                                                                  tCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACg                                <  2:1654500/150‑1 (MQ=255)
                                                                                                                   ccAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGc                               <  1:1745027/150‑1 (MQ=255)
                                                                                                                    cAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCa                              >  2:1019550/1‑150 (MQ=255)
                                                                                                                      ggTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAAt                            >  1:950236/1‑150 (MQ=255)
                                                                                                                       gTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAAtt                           >  2:1265332/1‑150 (MQ=255)
                                                                                                                        tAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTg                          <  1:1449457/150‑1 (MQ=255)
                                                                                                                             tATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGcc                     <  2:1388663/150‑1 (MQ=255)
                                                                                                                             tATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGcc                     <  1:845982/150‑1 (MQ=255)
                                                                                                                             tATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGcc                     <  1:119774/150‑1 (MQ=255)
                                                                                                                             tATTCGCCTCTTTCAGCGCCTGACTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGcc                     <  1:484496/150‑1 (MQ=255)
                                                                                                                              aTTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCa                    >  2:1613426/1‑150 (MQ=255)
                                                                                                                               ttCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAg                   <  1:1685217/150‑1 (MQ=255)
                                                                                                                                tCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGa                  <  2:876626/150‑1 (MQ=255)
                                                                                                                                      ggcaaaacgcCTGCAAGGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGCATCttt            >  1:1787340/8‑150 (MQ=255)
                                                                                                                                      ggcaaaacgcCTGCAAGGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCttt            >  1:1364689/8‑150 (MQ=255)
                                                                                                                                      ctTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCttt            >  2:28874/1‑150 (MQ=255)
                                                                                                                                      ctTTCAGCGCCGGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGAAGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCttt            <  2:308697/150‑1 (MQ=255)
                                                                                                                                       tttCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTg           <  2:927450/150‑1 (MQ=255)
                                                                                                                                        ttCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCCCACGAGACGCAATTGTCGCCAGAATCTTTgg          <  2:692119/150‑1 (MQ=255)
                                                                                                                                        ttCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAAGCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTgg          <  1:30178/150‑1 (MQ=255)
                                                                                                                                          cAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGgcg        >  2:1768836/1‑150 (MQ=255)
                                                                                                                                            gcgcCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCa      >  1:1127370/1‑150 (MQ=255)
                                                                                                                                            gcgcCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGGCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCa      <  1:1238011/150‑1 (MQ=255)
                                                                                                                                             cgcCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAg     <  1:387194/150‑1 (MQ=255)
                                                                                                                                             cgcCTGCCTTGCAGGCGTTTTGCCCGGGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAg     <  1:759853/150‑1 (MQ=255)
                                                                                                                                              gcctgcctTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAgg    <  2:1520679/150‑1 (MQ=255)
                                                                                                                                               cctgcctTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAggg   >  1:1249395/1‑150 (MQ=255)
                                                                                                                                               cctgcctTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAggg   >  2:1404688/1‑150 (MQ=255)
                                                                                                                                               cctgcctTGCAGGCGTTTTGCCCGTGGGTCTAGATAAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCGTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAggg   <  2:1373327/150‑1 (MQ=255)
                                                                                                                                                ctgcctTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGa  <  2:815525/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATCTGGCGCGATGCGATGAGTGAATAAACCCTGTAACATCTGGCGGTAGCAATTCACCATAATCCAGGTAGACTATTCGCCTCTTTCAGCGCCTGCCTTGCAGGCGTTTTGCCCGTGGGTCTGGATTAGAACACCGATGAATTTATTAAAATCGCTGGCCGCCGTCAGCTCGATGACCATGTTTTCGCGTGTGCTTGGCTTCGCACGAGACGCAATTGTCGCCAGAATCTTTGGCGCAGGGA  >  NC_000913/1127651‑1127944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: