Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,169,382 C→G 7.5% intergenic (+52/+30) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,169,3820CG 7.5% 349.2 / 17.1 118intergenic (+52/+30)bhsA/ldtCbiofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
Reads supporting (aligned to +/- strand):  ref base C (61/47);  new base G (5/4);  total (67/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01

Only 100 of 121 total aligned reads displayed.

GGCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCAT  >  NC_000913/1169234‑1169531
                                                                                                                                                    |                                                                                                                                                     
ggCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGcc                                                                                                                                                      >  1:581368/1‑150 (MQ=255)
ggCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGcc                                                                                                                                                      >  2:875905/1‑150 (MQ=255)
 gcgcAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCg                                                                                                                                                     >  1:636430/1‑150 (MQ=255)
 gcgcAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACAGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCg                                                                                                                                                     >  1:1737989/1‑150 (MQ=255)
  cgcAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTAATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCgt                                                                                                                                                    >  1:1175360/1‑150 (MQ=255)
      aaaaGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTTTAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTcg                                                                                                                                                <  1:781055/150‑1 (MQ=255)
         aGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCgg                                                                                                                                             <  1:160162/150‑1 (MQ=255)
          gCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACACGGCATGTCGCggg                                                                                                                                            <  1:1369767/150‑1 (MQ=255)
          gCGGATGAGATGGGCGCAAAATCTTTCCGGATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTATCCCCGCGACATGCCGTGTCGCggg                                                                                                                                            <  1:320578/150‑1 (MQ=255)
            ggATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGTGGa                                                                                                                                          >  1:645369/1‑150 (MQ=255)
                gcgaTGGGCGCAAAAGCTGTTCGTATTACTTTTGTATCCGGTCCGTATACCCTCCATCGATCAGCAGTAATTTATAAATAAGCCTTATCCCTGATTATTGCCTGGGACTGGTGATTTTTTCCCCGCGCCATGCCGTGTCGCGGGGAtttt                                                                                                                                      <  2:1199047/148‑1 (MQ=255)
                 agaTGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAttttt                                                                                                                                     <  1:774080/150‑1 (MQ=255)
                  gaTGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCGTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAtttttt                                                                                                                                    >  2:327058/1‑150 (MQ=255)
                  gaTGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGAtttttt                                                                                                                                    <  1:710070/150‑1 (MQ=255)
                    tGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGGCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTa                                                                                                                                  <  2:284592/150‑1 (MQ=255)
                    tGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTACTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTa                                                                                                                                  >  1:1701815/1‑150 (MQ=255)
                        cgaaaatTCTTTCCTTATTACTTCTGTAACCGGTCCTAATACCCTCCATGGAACTTCAGTATTTTAATATTATGCATTAACCCTCATTTATGCCTGCTACGGCTTATTTTTACGCCGCGACATGCCGTGTCGCGGTGATTTTTTTTTCCg                                                                                                                              <  2:1048130/147‑1 (MQ=255)
                         gcAAAATCTTTCCGTTTTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCgg                                                                                                                             <  1:274748/150‑1 (MQ=255)
                          cAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCAAGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCggg                                                                                                                            >  1:155768/1‑150 (MQ=255)
                           aaaaTCTTTCCGTATTACTTCTGTAACCGGTGCGAATACCCTCCATGGATCAGCATTAATTTATAAATTAGCCTTAACGCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGc                                                                                                                           <  2:1282845/150‑1 (MQ=255)
                              aTCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCttt                                                                                                                        >  2:1830271/1‑150 (MQ=255)
                                   tCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGc                                                                                                                   <  2:1869612/150‑1 (MQ=255)
                                   tCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGc                                                                                                                   >  2:524571/1‑150 (MQ=255)
                                     cGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTTTCCGGGCTTTACAGCGt                                                                                                                 >  1:1479215/1‑150 (MQ=255)
                                     agTATTACTTCTGTAACCGTTACGAATAACCTCGATGGAACAGATGTAATTTATAAATAAGCATTAAACCTAATTAATGCCTTCTACTGCTGATTTTTTCCCCGAGACATGCCTTGTATCGTGTATTTTTTTATCAGGGCTTTACAGCGt                                                                                                                 <  2:484362/149‑1 (MQ=255)
                                        aTTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAATCATTAACCCTCATTAATGCCTGCTACTGCTGTGTTTTTCCCCTCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGGTTTACAGCGTTtg                                                                                                              <  2:297353/150‑1 (MQ=255)
                                         ttACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTtgt                                                                                                             >  1:1423738/1‑150 (MQ=255)
                                           aCTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTgg                                                                                                           >  1:1710915/1‑150 (MQ=255)
                                            cTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTggg                                                                                                          >  1:877250/1‑150 (MQ=255)
                                            cTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTggg                                                                                                          >  2:847636/1‑150 (MQ=255)
                                              tCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCt                                                                                                        >  2:686777/1‑150 (MQ=255)
                                               cTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTc                                                                                                       >  2:1550921/1‑150 (MQ=255)
                                                tGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCa                                                                                                      >  1:1430220/1‑150 (MQ=255)
                                                  tAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCCATAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACt                                                                                                    <  2:286103/150‑1 (MQ=255)
                                                    aCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGCCAGGCAGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGGTTGTGGGCTCACTtg                                                                                                  >  1:904873/1‑150 (MQ=255)
                                                    aCCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtg                                                                                                  <  2:131951/150‑1 (MQ=255)
                                                       ggTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgatg                                                                                               <  2:373365/150‑1 (MQ=255)
                                                       ggTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgatg                                                                                               <  2:1403294/150‑1 (MQ=255)
                                                        gTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTtgatga                                                                                              >  2:1720287/1‑150 (MQ=255)
                                                           cGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcgg                                                                                           >  2:1556101/1‑150 (MQ=255)
                                                             aaTACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTCCTGCTGATTTTTTCCCCGCGACATCCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcg                                                                                         <  2:336449/150‑1 (MQ=255)
                                                              aTACCCTCCATGGAACAGCATTAATTTATAAATAAGCATTAACCCTCATTAATTCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGAcggcgg                                                                                        <  1:1210055/150‑1 (MQ=255)
                                                                  ccTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTGTTTCCCCGCGACATGCCGGGTCGCGGGGATGGTTTTATCCGGGCTTTACAGCGTGTGTGGGCTCACTTGATGCCGGCGGACAt                                                                                    >  1:1303754/1‑150 (MQ=255)
                                                                   cTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATc                                                                                   >  1:437616/1‑150 (MQ=255)
                                                                    tCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATcc                                                                                  >  1:464039/1‑150 (MQ=255)
                                                                    tCCATGGAACAGCAGTAATGTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGCCATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGGTCCCTTGATGACGGCGGACATcc                                                                                  >  2:343197/1‑150 (MQ=255)
                                                                      cATGGAACAGCATAAATTTATAAATAAGCATTAACCCTCATTAATGCCTGGTACTGCTGATTTTTTCCCCGCGACATGCCGAGGCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCAc                                                                                <  1:1744864/150‑1 (MQ=255)
                                                                         ggAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGCCATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCgg                                                                             >  2:1554863/1‑150 (MQ=255)
                                                                              cgactgaaTTTATAACTAAGCATAACCCCTCATAAATGCCTGCGACTGCTTATTATTTCCCCGCGACATGCCGTGGCGCGGTGATTTTTATATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGTGGATGTCCGCCGTCATca                                                                        <  2:301355/144‑2 (MQ=255)
                                                                              agcagTAATTTATAAATAAGCATTAACCCTCATTACTGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGTGGATGTCCGCCGTCATca                                                                        <  1:833887/150‑2 (MQ=255)
                                                                                     aTTTATAAATACGCCCTACCCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGGCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGTCATCCCGGAACg                                                                 <  1:15963/150‑1 (MQ=255)
                                                                                     aTTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACg                                                                 <  1:493288/150‑1 (MQ=255)
                                                                                      tttATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACgg                                                                <  2:4430/150‑1 (MQ=255)
                                                                                           aaataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatc                                                           <  2:1737989/150‑1 (MQ=255)
                                                                                           aaataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatc                                                           <  2:636430/150‑1 (MQ=255)
                                                                                            aatacGCAATAACCCTCATTAATGCCTGCTCCTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatcc                                                          <  2:349564/150‑1 (MQ=255)
                                                                                            aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatcc                                                          >  2:860526/1‑150 (MQ=255)
                                                                                            aataaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatcc                                                          >  1:74489/1‑150 (MQ=255)
                                                                                              taaGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatccat                                                        >  2:395135/1‑150 (MQ=255)
                                                                                               aaGCATTAACCATCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatccatc                                                       <  2:558371/150‑1 (MQ=255)
                                                                                                aGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGAcatccatcc                                                      >  2:1655534/1‑149 (MQ=255)
                                                                                                 gCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATcac                                                     >  2:14828/1‑150 (MQ=255)
                                                                                                  cATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGGCATCCACCGGCATCCCGGAACGGACATCCATcaca                                                    >  2:357017/1‑150 (MQ=255)
                                                                                                   aTTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCCCCGGCATCCCGGAACGGACCTCCATCACAt                                                   >  2:1687585/1‑150 (MQ=255)
                                                                                                     tAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTTTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGt                                                 <  2:1782794/150‑1 (MQ=255)
                                                                                                      aaCCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGtt                                                <  2:1143459/150‑1 (MQ=255)
                                                                                                      aaCCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGtt                                                <  2:464039/150‑1 (MQ=255)
                                                                                                        cccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGc                                              >  1:524750/1‑150 (MQ=255)
                                                                                                         ccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCa                                             >  1:751235/1‑150 (MQ=255)
                                                                                                         ccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCa                                             >  1:1793767/1‑150 (MQ=255)
                                                                                                         ccTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGACGTGTCGCGGGGATTTTTTTATCAGGGCTTTACAGCTTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCa                                             >  1:354327/1‑150 (MQ=255)
                                                                                                             aTTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCAc                                         >  1:661185/1‑150 (MQ=255)
                                                                                                                 aTGGCTGCTACTGCTGATTTTTTGCCCGCGAGATGCCGTGGCGCGGGGATTTTTTTATGCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCa                                     <  1:1258320/150‑1 (MQ=255)
                                                                                                                   gcatGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTAGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGc                                   <  1:327058/147‑1 (MQ=255)
                                                                                                                   gCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGc                                   <  1:568495/150‑1 (MQ=255)
                                                                                                                   gCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACAGCCATCACATGTTGCATCCCTTCAGc                                   >  1:62975/1‑150 (MQ=255)
                                                                                                                    ccTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCg                                  <  1:1873267/150‑1 (MQ=255)
                                                                                                                    ccTGCTACTGCTGATTTTTTCCCCGCGACATGCCGGGGCGGGGGGAATTTTTTATCCGGGCGTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACCTACACCCGCCTACCGGAACGGACCTACATGACCTGTTTGATCACGTGAGCg                                  >  2:1635640/1‑150 (MQ=255)
                                                                                                                             gctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGATATCCATCACATGTTGCATCACTTCAGCGTCAGTTtgt                         >  1:677973/1‑150 (MQ=255)
                                                                                                                             gctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTtgt                         >  1:359777/1‑150 (MQ=255)
                                                                                                                             gctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTtgt                         >  2:1211222/1‑150 (MQ=255)
                                                                                                                              ctgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTtgtg                        <  2:581368/150‑1 (MQ=255)
                                                                                                                               tgATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTCTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGCCGGCGGACATCCACCGGCATCCCGGAACGGACCTCCATCACATGTTGCATCACTGCAGCGTCAGTTTGtgc                       >  1:959129/1‑150 (MQ=255)
                                                                                                                                gATTTTTTCCCCGCGACATGGCGTGTAGCGGGGATTTTTTTATCCGGGATTTACAGCGTTAGTGGGCTCACTTGATGAAGGCGGACATCCACCGGCATCCCGGAACGGACATCCATAACATGTTGCATCACTTCAGCGTCAGTTTGtgct                      >  1:1803638/1‑150 (MQ=255)
                                                                                                                                gATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGtgct                      <  2:877250/150‑1 (MQ=255)
                                                                                                                                gATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGtgct                      >  1:1084425/1‑150 (MQ=255)
                                                                                                                                  aaaaatCCCCGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGtgctgc                    >  2:174928/6‑150 (MQ=255)
                                                                                                                                   tttttCCCCGCGACATGCCGTGGCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCa                   <  1:183350/150‑1 (MQ=255)
                                                                                                                                     aatCCCCGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATc                 >  1:1334394/3‑150 (MQ=255)
                                                                                                                                         cccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTa             >  2:1292031/1‑150 (MQ=255)
                                                                                                                                         cccGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTa             <  1:870534/150‑1 (MQ=255)
                                                                                                                                         cccGCGACACGGCATGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCACGGAACGGACCTCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTa             >  1:320536/1‑150 (MQ=255)
                                                                                                                                           cgcgACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTaaa           >  2:1007759/1‑150 (MQ=255)
                                                                                                                                            gccaCATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAAt          <  2:155768/147‑1 (MQ=255)
                                                                                                                                             cgACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTTCATCACTTCCGCGTCAGTTTGTGCTGCATCTTTAAATg         >  1:284709/1‑150 (MQ=255)
                                                                                                                                               aCATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGat       <  1:1550921/150‑1 (MQ=255)
                                                                                                                                                cctGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTGGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGatt      <  2:1175360/148‑1 (MQ=255)
                                                                                                                                                 aTGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGattg     <  1:1714390/150‑1 (MQ=255)
                                                                                                                                                   gCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGattgca   >  2:1814326/1‑150 (MQ=255)
                                                                                                                                                    ccGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGattgcat  <  1:1556101/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGCGCAAAAAGCGGATGAGATGGGCGCAAAATCTTTCCGTATTACTTCTGTAACCGGTCCGAATACCCTCCATGGAACAGCAGTAATTTATAAATAAGCATTAACCCTCATTAATGCCTGCTACTGCTGATTTTTTCCCCGCGACATGCCGTGTCGCGGGGATTTTTTTATCCGGGCTTTACAGCGTTTGTGGGCTCACTTGATGACGGCGGACATCCACCGGCATCCCGGAACGGACATCCATCACATGTTGCATCACTTCAGCGTCAGTTTGTGCTGCATCTTTAAATGATTGCAT  >  NC_000913/1169234‑1169531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: