Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,223,931 C→A 6.3% intergenic (+24/+348) ycgI → / ← minE pseudogene/cell division topological specificity factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,223,9310CA 6.3% 391.5 / 7.9 136intergenic (+24/+348)ycgI/minEpseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand):  ref base C (64/62);  new base A (7/3);  total (71/65)
Fisher's exact test for biased strand distribution p-value = 3.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

TACCAGGCAGCTATTAAAGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACA  >  NC_000913/1223782‑1224077
                                                                                                                                                     |                                                                                                                                                  
taCCAGGCAGCTATTAAAGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTc                                                                                                                                                    >  2:828433/1‑150 (MQ=255)
   gaGGCAGCTATTAAAGCCTGATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGgc                                                                                                                                                 <  2:1203593/149‑1 (MQ=255)
   ccggCCGCTATTAAAGCCTCATGCAGCTGTACGTTTAGCGGACATCTCGGGGTGGTGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTGCGgc                                                                                                                                                 <  2:1404072/148‑1 (MQ=17)
   cAGGCAGCTATTAAAGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGgc                                                                                                                                                 >  1:978224/1‑150 (MQ=255)
    aGGCAGCTATTAAAGCCTCAATCAGCAGTACGTGTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGgcg                                                                                                                                                <  2:1531886/150‑1 (MQ=255)
      gCAGCTAGTAAAGCCTCCTTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGGGTGGAATCCCCGTGGAGCGCGGTGGCGGGTGTGAACTGGTCGTTCTGACCATCAACGCTTAAACTGCGCTTCGgcgcg                                                                                                                                              >  2:1770950/1‑149 (MQ=18)
       cAGCTATTAAAGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAg                                                                                                                                             >  2:30553/1‑150 (MQ=255)
       cAGCTATTAAAGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAg                                                                                                                                             >  1:1848124/1‑150 (MQ=255)
       cAGCTATTAAAGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAg                                                                                                                                             >  1:1690036/1‑150 (MQ=255)
         gCTATTAAAGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGtt                                                                                                                                           >  2:764629/1‑150 (MQ=255)
          cTATTAAAGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCCGAAGCGCAGttt                                                                                                                                          <  1:634146/150‑1 (MQ=255)
          cTATTAAAGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGGGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCCGAAGCGCAGttt                                                                                                                                          <  1:1395384/150‑1 (MQ=17)
            aTTAAAGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTc                                                                                                                                        >  2:1714037/1‑150 (MQ=255)
            aTTAAAGCCTCATTCAGCAGTACGTTAAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTc                                                                                                                                        <  1:225468/150‑1 (MQ=255)
              tAAAGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGt                                                                                                                                      >  1:830713/1‑150 (MQ=255)
              tAAAGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGt                                                                                                                                      >  1:543595/1‑150 (MQ=255)
              tAAAGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGt                                                                                                                                      <  1:711068/150‑1 (MQ=255)
               aaaGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGATCGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTCCGCTTCGGCGCAGTTTTCGtt                                                                                                                                     >  2:924929/1‑150 (MQ=255)
               aaaGCCTCATTCACCAGTACGTTTAGCGGACATCGCGGGGTGGGGTCTTGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAATCTGCGCTTCGTCGCAGTTTTCGtt                                                                                                                                     <  2:1477821/150‑1 (MQ=18)
                aaGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGttt                                                                                                                                    <  2:1451515/150‑1 (MQ=255)
                 aGCCTCTTTCCGCAGTTCGTTTATCGGAAATCTCGGGGTTGGGTATAGCCATGGTGGCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGGGTGATCTGGTCGGTCTGACCTTCATCTATTAAACTGCGCTTCGGCGCAGTTTTCGTTTa                                                                                                                                   <  1:893927/150‑1 (MQ=18)
                 aGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTa                                                                                                                                   <  1:1278375/150‑1 (MQ=255)
                  gCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTAc                                                                                                                                  >  1:552864/1‑150 (MQ=255)
                   ccTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACa                                                                                                                                 <  2:829393/150‑1 (MQ=255)
                     tCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAgg                                                                                                                               >  2:877843/1‑150 (MQ=255)
                         tCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGt                                                                                                                           <  1:877843/150‑1 (MQ=255)
                         tCAGCAGTACGTGTAGCGGACAGCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCTTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGt                                                                                                                           <  1:148445/150‑1 (MQ=255)
                          cagcagTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGtt                                                                                                                          >  2:1806046/1‑150 (MQ=255)
                            gcagTACGTTTAGCGGACATCTCGGGGAGGGGTATAGCCATGGTGCCGGTGAGGAATCCCCGTGGAACGCGGTGGCTGGAGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGAGCTACGGCGCAGTTTACGTATACAGGATGttca                                                                                                                        >  1:218476/1‑148 (MQ=18)
                                   gTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGaa                                                                                                                 >  1:1635948/1‑150 (MQ=255)
                                    tttAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGACCGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGaaa                                                                                                                >  1:1057928/1‑150 (MQ=255)
                                    tttAGCGGACATCTCGGGGTGGGGTAATGCAATGGTGCCTGTGTGTAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCAGAGCATCAACGATTAAACTGCGCTGCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGaaa                                                                                                                <  1:100101/150‑1 (MQ=25)
                                           gACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTgg                                                                                                         <  1:777913/150‑1 (MQ=255)
                                            aCATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTggg                                                                                                        <  1:1732065/150‑1 (MQ=255)
                                            aCATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTggg                                                                                                        <  2:1121664/150‑1 (MQ=255)
                                              aTCTCGGGGTGGGTTATAGCCATGGTGACGGTGAGGAATCCCAGTGGAACGCGGTGGCTGGTGTGAACTGTTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTgggcc                                                                                                      >  2:64852/1‑148 (MQ=255)
                                              aTCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGc                                                                                                      <  2:804950/150‑1 (MQ=255)
                                               tctcGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCa                                                                                                     <  1:142194/150‑1 (MQ=255)
                                                ctcGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAATGGAAAATTCTGGGGCaa                                                                                                    >  1:158373/1‑150 (MQ=255)
                                                ctcGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCaa                                                                                                    >  1:36075/1‑150 (MQ=255)
                                                 tcGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCaaa                                                                                                   >  1:1547967/1‑150 (MQ=255)
                                                  cGGGGTGGGGTATAGCCATGGTGCAGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGCCCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCaaaa                                                                                                  >  2:1008555/1‑150 (MQ=255)
                                                   ggggtggggtATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGCCCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCaaaaa                                                                                                 >  1:290354/1‑150 (MQ=255)
                                                      gtggggtATAGCCTTGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTTGCTGGTGTGTACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAg                                                                                              <  2:627933/150‑1 (MQ=255)
                                                        ggggtATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGcc                                                                                            >  2:1445279/1‑150 (MQ=255)
                                                         gtgtATAGCCATGGTGCCGGTGTGGAATCCCCGTGTAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGccc                                                                                           <  2:795886/148‑1 (MQ=255)
                                                         gtgtATAGCCATGGTGCCGGGGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTATGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGccc                                                                                           <  2:802743/148‑1 (MQ=255)
                                                          ggtATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGcccc                                                                                          >  1:156838/1‑149 (MQ=255)
                                                               aGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGt                                                                                     >  2:1776591/1‑150 (MQ=255)
                                                                     ggTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTAcggc                                                                               <  2:1635948/150‑1 (MQ=255)
                                                                      gTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCCTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTAcggcg                                                                              <  2:969952/150‑1 (MQ=255)
                                                                        gCCGGTGTGGAATCCGCGGGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGAACATCAACGATTACACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCggg                                                                            <  2:219155/150‑1 (MQ=255)
                                                                        gCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCggg                                                                            >  1:1060195/1‑150 (MQ=255)
                                                                        gCCGGTGTGGAATCCCCGTGGAACGCGGTGGCGGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCggg                                                                            <  1:622693/150‑1 (MQ=255)
                                                                          cGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGaa                                                                          >  1:1612360/1‑150 (MQ=255)
                                                                           ggTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGTTGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGaaa                                                                         >  2:1451030/1‑150 (MQ=255)
                                                                           ggTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGaaa                                                                         <  1:1843970/150‑1 (MQ=255)
                                                                            gtgtGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAAc                                                                        <  1:1445279/150‑1 (MQ=255)
                                                                             ggtgGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGAGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAAcc                                                                       <  1:1825505/149‑1 (MQ=255)
                                                                              gtgGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCt                                                                      >  2:614192/1‑150 (MQ=255)
                                                                               gggAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTTGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTc                                                                     <  1:1308702/149‑1 (MQ=255)
                                                                                     ccccGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTa                                                               >  1:923463/1‑150 (MQ=255)
                                                                                      cccGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCCGTTATCGTTTACAGGATGTTGAAAGGGATAATTCTGGGGCATAAAAAGCCAGCCAGTTACGGCGGGAAACCTCATCCTAt                                                              >  2:1147677/1‑150 (MQ=38)
                                                                                       ccGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATg                                                             >  2:845433/1‑150 (MQ=255)
                                                                                       ccGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATg                                                             <  1:387785/150‑1 (MQ=255)
                                                                                       ccGGGGAACGCGGGGGCTGGGGTGAACTGGGCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATg                                                             <  2:123515/150‑1 (MQ=255)
                                                                                        cggggAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATgg                                                            <  2:1525902/147‑1 (MQ=255)
                                                                                         gTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTACACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGGTACGGCGGGAAACCTCATCCTATggg                                                           <  1:1368642/150‑1 (MQ=255)
                                                                                           ggAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGgag                                                         <  1:1001666/150‑1 (MQ=255)
                                                                                            gAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGgaga                                                        >  2:8444/1‑150 (MQ=255)
                                                                                                  ggTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATg                                                  <  1:1635025/150‑1 (MQ=255)
                                                                                                    tGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGaa                                                <  2:543595/150‑1 (MQ=255)
                                                                                                    gggCTGGTGTGAACTGTTAGTTCTGAACATCAACGATTAAACTGCGCTTCGGCTCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGaa                                                <  2:826928/149‑1 (MQ=255)
                                                                                                      gCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGaata                                              <  1:1353365/150‑1 (MQ=255)
                                                                                                       cTGGTGAGAACTGGTCGTTCTGACCATCAACTATTAAACTGCGCTTCGCCGCATTTTTCGTTTACAGGATGTTGAATGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGCCGGGAAACCTCATCCAATGGGAGATCAATGaataa                                             <  2:492929/150‑1 (MQ=255)
                                                                                                        tGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGaataat                                            <  2:487408/150‑1 (MQ=255)
                                                                                                          gtgtGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGa                                          <  2:1084815/150‑1 (MQ=255)
                                                                                                           tgtgAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGaa                                         >  2:1668475/1‑150 (MQ=255)
                                                                                                           tgtgAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGCGAGAACAATGAATAATGaa                                         >  2:608829/1‑150 (MQ=255)
                                                                                                              gAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAAtt                                      >  1:260599/1‑150 (MQ=255)
                                                                                                                   ggTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGATTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTAATCCTATGGGAGAACAATGAATAATGAAATTGCggg                                 >  1:477231/1‑150 (MQ=255)
                                                                                                                       gTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTa                             >  1:1790668/1‑150 (MQ=255)
                                                                                                                         tCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTatc                           <  1:1030054/150‑1 (MQ=255)
                                                                                                                                aTCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTccc                    <  2:1690036/150‑1 (MQ=255)
                                                                                                                                aTCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTccc                    <  2:1547967/150‑1 (MQ=255)
                                                                                                                                aTCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTccc                    >  1:1546903/1‑150 (MQ=255)
                                                                                                                                  cAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCTTCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAg                  >  2:1476142/1‑150 (MQ=255)
                                                                                                                                  cAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAg                  >  2:969893/1‑150 (MQ=255)
                                                                                                                                     cGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGtat               >  2:1286485/1‑150 (MQ=255)
                                                                                                                                     aacgaaaaCTGCGCCGAAGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACTGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGtat               >  2:881313/6‑150 (MQ=255)
                                                                                                                                     aacgaaaaCTGCGCCGAAGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGtat               >  1:766888/6‑150 (MQ=255)
                                                                                                                                       aTTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTatat             >  1:589718/1‑150 (MQ=255)
                                                                                                                                        ttAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATc            <  1:1806046/150‑1 (MQ=255)
                                                                                                                                          aaaCTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCa          <  2:1389177/150‑1 (MQ=255)
                                                                                                                                           aaCTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCAt         >  2:1150117/1‑150 (MQ=255)
                                                                                                                                             cTGCGCTTCGGCGCAGTTTTGGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATAc       >  1:427228/1‑150 (MQ=255)
                                                                                                                                             cTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATAc       >  1:163448/1‑150 (MQ=255)
                                                                                                                                                 gcTTCGTCGAAGTTTTCGTATACAGGATGTTGATAGGGAAAAGTCTGGGTCATAAAAATCCCGCCAGTTACGGCGGGAAACCTCATCCTATCGGAGAACAAAGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAAc   <  1:109605/150‑1 (MQ=255)
                                                                                                                                                  cTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACa  <  2:1645486/150‑1 (MQ=255)
                                                                                                                                                  cTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACa  <  2:1060195/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TACCAGGCAGCTATTAAAGCCTCATTCAGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAGTTTTCGTTTACAGGATGTTGAAAGGGAAAATTCTGGGGCAAAAAAAGCCCGCCAGTTACGGCGGGAAACCTCATCCTATGGGAGAACAATGAATAATGAAATTGCGGGGTTATCATCTCCCAGTATATCCATACTAACA  >  NC_000913/1223782‑1224077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: