Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,231,963 T→A 5.4% Y260N (TAT→AAT)  umuC → translesion error‑prone DNA polymerase V subunit; DNA polymerase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,231,9630TA 5.4% 318.9 / 4.8 106Y260N (TAT→AAT) umuCtranslesion error‑prone DNA polymerase V subunit; DNA polymerase activity
Reads supporting (aligned to +/- strand):  ref base T (56/44);  new base A (3/3);  total (59/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCG  >  NC_000913/1231817‑1232112
                                                                                                                                                   |                                                                                                                                                     
tCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATc                                                                                                                                                    <  1:402070/150‑1 (MQ=255)
    gTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTc                                                                                                                                                <  1:428551/150‑1 (MQ=255)
       tATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACACTGTTTGCAACTGGAAGAGTTTGTACCGACGAAGCAGGAAATTATGTGTTCCGGCTCGTTTGGTGAACGCATCACGGATTATCCCTCGAt                                                                                                                                             >  2:465014/1‑150 (MQ=255)
        aTCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGACGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATg                                                                                                                                            <  2:580249/150‑1 (MQ=255)
         tCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGc                                                                                                                                           >  2:430368/1‑150 (MQ=255)
          ccGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCg                                                                                                                                          >  2:1058468/1‑150 (MQ=255)
           cGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCgg                                                                                                                                         >  1:310744/1‑150 (MQ=255)
           cGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCgg                                                                                                                                         >  1:110917/1‑150 (MQ=255)
           cGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACACTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCgg                                                                                                                                         >  1:170161/1‑150 (MQ=255)
            gTAAACATTTTAATGTCGTTCGAGAAAGAACGGTGAGTGAACTGCGCGGCGAACCCTGTTTGCACCTGGAAGAGTTTGCACCGACGACGCAGGAAATTATCTGTTACCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGc                                                                                                                                        <  2:1139228/150‑1 (MQ=255)
            gTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGc                                                                                                                                        <  1:810184/150‑1 (MQ=255)
            gTAAACATTTTAATGTCGTGCTCGAAAGAACGGAGCGTGAACTGCGCGGCGAACCCTGTATGCAACTGGAAGAGTTTGCACGGAGGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCCTCACGGAGTATCCGTCGATGCGGc                                                                                                                                        >  1:336590/1‑150 (MQ=255)
             tAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGTCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCa                                                                                                                                       >  1:219927/1‑150 (MQ=255)
             tAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCGACGGATAATCCGTGATGCgttc                                                                                                                                       <  1:662624/150‑4 (MQ=255)
               aaCATTTTAATGTCGTGCACGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCGACGGATAATCCGTGATGCgttcac                                                                                                                                     <  2:164138/150‑6 (MQ=255)
                 cATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGcc                                                                                                                                   >  1:900744/1‑150 (MQ=255)
                 cATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGcc                                                                                                                                   >  1:1231992/1‑150 (MQ=255)
                   ttttAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCAt                                                                                                                                 >  1:458334/1‑150 (MQ=255)
                        aTGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTa                                                                                                                            <  1:1731121/150‑1 (MQ=255)
                          gTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGt                                                                                                                          <  1:747663/150‑1 (MQ=255)
                            cGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTa                                                                                                                        <  2:946720/150‑1 (MQ=255)
                            cGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTa                                                                                                                        <  1:1768408/150‑1 (MQ=255)
                               gCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGc                                                                                                                     >  1:840901/1‑150 (MQ=255)
                                cTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGATATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCt                                                                                                                    >  2:590103/1‑150 (MQ=255)
                                cTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCt                                                                                                                    <  2:1094878/150‑1 (MQ=255)
                                  cGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGc                                                                                                                  >  1:1387790/1‑150 (MQ=255)
                                     aaGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCg                                                                                                               <  1:1119144/150‑1 (MQ=255)
                                         aCGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATAATCTGTTCCCGCTCGTGTGGTGAACGCATCACGGATTATCCGGCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGggcg                                                                                                           <  1:913145/150‑1 (MQ=255)
                                          cGGTGCGTGAACTGCGCGGCGCACCCTGTTTGCAACTGTACGAGTTTGCACCGACGAAGCAGGAAATTATCTGATCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGggcgg                                                                                                          <  1:338346/150‑1 (MQ=255)
                                          cGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGggcgg                                                                                                          >  1:1712790/1‑150 (MQ=255)
                                               cGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaa                                                                                                     >  2:1578547/1‑150 (MQ=255)
                                               cGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGAATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaa                                                                                                     >  2:225555/1‑150 (MQ=255)
                                                gTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGTATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaaa                                                                                                    <  2:1186385/150‑1 (MQ=255)
                                                 tGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaaaa                                                                                                   >  1:1626382/1‑150 (MQ=255)
                                                 tGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGaaaa                                                                                                   <  2:151023/150‑1 (MQ=255)
                                                   aaCTGCGCGGCGAACCCTGTTTGCTACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAAc                                                                                                 <  2:788796/150‑1 (MQ=255)
                                                    acggcgcGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACt                                                                                                <  1:1724907/147‑1 (MQ=255)
                                                    aCTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACt                                                                                                <  1:1392326/150‑1 (MQ=255)
                                                      tGCGCGGCGAACCCTGTATGCAACAGGAAGAGTTAGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTc                                                                                              <  2:290780/150‑1 (MQ=255)
                                                       gcgcGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCg                                                                                             <  2:744805/150‑1 (MQ=255)
                                                       gcgcGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATAATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCg                                                                                             <  1:1445213/150‑1 (MQ=255)
                                                        cgcgGCGAACCCTGTTTGCAACTGGCAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGAAGTTACGCTGCCCGTGCGGCGGAAAAACTTCGc                                                                                            <  1:365587/150‑1 (MQ=255)
                                                        cgcgGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGc                                                                                            <  2:1520747/150‑1 (MQ=255)
                                                                   cTGTTAGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATAATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCa                                                                                 <  2:601671/150‑1 (MQ=255)
                                                                    agTTTGCAACTGGAAGAGTTTGCACCGACGAAGAAGGAAAGTATCTGTTCCCGCGCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGAAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTGCGCAGCGAGCATCaa                                                                                >  2:456416/2‑150 (MQ=255)
                                                                     gTTTGCACCTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAat                                                                               <  2:750068/150‑1 (MQ=255)
                                                                     gTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAat                                                                               <  2:756600/150‑1 (MQ=255)
                                                                      tttGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAata                                                                              <  2:219927/150‑1 (MQ=255)
                                                                         gCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACTCATCACGGATTATCCGTCGATTCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTg                                                                           <  1:341158/150‑1 (MQ=255)
                                                                         gCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCAGTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTg                                                                           >  1:1401547/1‑150 (MQ=255)
                                                                          cAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGt                                                                          >  1:62926/1‑150 (MQ=255)
                                                                           aaCTGGAAGATTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTc                                                                         <  1:1365498/150‑1 (MQ=255)
                                                                           aaCTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCACAGCGAGCATCAATATTGTc                                                                         <  2:848658/150‑1 (MQ=255)
                                                                               ggAATAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGTCCTTTTGTAGTTAAGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCAGCAATATTGTCGGtt                                                                     >  1:270620/1‑150 (MQ=255)
                                                                               ggAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGtt                                                                     >  1:710785/1‑150 (MQ=255)
                                                                               ggAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGtt                                                                     >  2:1729295/1‑150 (MQ=255)
                                                                                gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGttt                                                                    >  2:1848419/1‑150 (MQ=255)
                                                                                gAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGttt                                                                    >  2:813328/1‑150 (MQ=255)
                                                                                  agagTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTAt                                                                  >  1:386611/1‑150 (MQ=255)
                                                                                   gagTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTAtc                                                                 <  2:110917/150‑1 (MQ=255)
                                                                                     gTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGTTCGCATCACGGATTATCGGTAGATGCGGCAGGCCATTTGTAGTTACGCTGCCGGGGCGGCGGAAATCCTTCGCAGCGAGCATCAATATTGTCGGttttagtc                                                               >  2:253138/1‑145 (MQ=255)
                                                                                     gTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTAtctc                                                               >  1:59842/1‑150 (MQ=255)
                                                                                     gTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTAGTAGTTACGCTGCCCGGGCGGCGGAAAACCTTCGCAGCGAGCATCAAGATTGTCGGTTTAgctc                                                               >  2:278591/1‑150 (MQ=255)
                                                                                           aCCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGtt                                                         >  2:1557964/1‑150 (MQ=255)
                                                                                             cgacgaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTtta                                                       <  2:1648502/150‑1 (MQ=255)
                                                                                                 gaAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTaa                                                   <  2:577796/150‑1 (MQ=255)
                                                                                                  caGCAGGAAATTATCTGCTCCCGCTCCGTGGGTGAACGCATCACGGAGTATCCGTCGATGCGGCAGGCCATATGTAGGTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGGTTATCTCCACGTTTATTAAg                                                  <  2:850280/149‑1 (MQ=255)
                                                                                                  aaGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAg                                                  <  1:1576894/150‑1 (MQ=255)
                                                                                                      aGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGt                                              >  1:838728/1‑150 (MQ=255)
                                                                                                      aGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGt                                              >  2:1536183/1‑150 (MQ=255)
                                                                                                       ggAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTc                                             >  2:640940/1‑150 (MQ=255)
                                                                                                         aaaTTATCTGTTCCCGCTCGTTTTGTGAACGCATCAAGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCAc                                           <  2:448321/150‑1 (MQ=255)
                                                                                                             tATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCCCCAtt                                       >  2:1107449/1‑150 (MQ=255)
                                                                                                                  gTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTgcgc                                  <  2:63892/150‑1 (MQ=255)
                                                                                                                   ttCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCt                                 <  1:1508063/150‑1 (MQ=255)
                                                                                                                       cGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAAt                             >  1:359278/1‑150 (MQ=255)
                                                                                                                        gCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATg                            <  2:1327335/150‑1 (MQ=255)
                                                                                                                            gTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAAcc                        >  1:421287/1‑150 (MQ=255)
                                                                                                                               tGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCtta                     >  2:394109/1‑150 (MQ=255)
                                                                                                                               tGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCtta                     <  1:867045/150‑1 (MQ=255)
                                                                                                                               tGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCtta                     >  1:1067317/1‑150 (MQ=255)
                                                                                                                                ggTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCttat                    >  2:1588806/1‑150 (MQ=255)
                                                                                                                                  tGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCttatta                  >  2:1785092/1‑150 (MQ=255)
                                                                                                                                    gccGCATCGACGGATAATCCGTGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACg                >  2:1622705/3‑150 (MQ=255)
                                                                                                                                     ccGCATCGACGGATAATCCGTGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACgg               >  2:1604159/2‑150 (MQ=255)
                                                                                                                                      cGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGc              <  1:813328/150‑1 (MQ=255)
                                                                                                                                      cGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGc              >  2:1864015/1‑150 (MQ=255)
                                                                                                                                       gcatcgaCGGATAATCCGTGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTAGTAAGACGTCCCCATTTGCGCTCAATGACCCTTATTACGGCa             >  2:742904/7‑150 (MQ=255)
                                                                                                                                       gCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCa             >  2:48813/1‑150 (MQ=255)
                                                                                                                                        cATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCaa            >  2:1414281/1‑150 (MQ=255)
                                                                                                                                          tCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATa          >  1:774094/1‑150 (MQ=255)
                                                                                                                                           cACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTCCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAg         >  1:1437609/1‑150 (MQ=255)
                                                                                                                                              cGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAgcg       <  1:1025482/150‑1 (MQ=255)
                                                                                                                                              cGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAgcg       <  1:1028576/150‑1 (MQ=255)
                                                                                                                                               ggATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCTGTTTATCTCCACGTTTATTAAGACGTCACCATATGCGCTCAATGAACCTTATTACGGCAATAgcgc      >  2:1670066/1‑150 (MQ=255)
                                                                                                                                               ggATTATCCGTCGATGCGGCAGGCCATATGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTAATTAAGACGTCACCATTTGCGCTCAAAGAACCTTATTACGGCAATAgcgc      >  2:1662581/1‑150 (MQ=255)
                                                                                                                                                gATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGcgcg     >  1:39515/1‑150 (MQ=255)
                                                                                                                                                  ttATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTc   <  2:458334/150‑1 (MQ=255)
                                                                                                                                                  ttATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTc   <  2:170161/150‑1 (MQ=255)
                                                                                                                                                   tATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCg  <  2:1787797/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TCCGGTTTATCCGTAAACATTTTAATGTCGTGCTCGAAAGAACGGTGCGTGAACTGCGCGGCGAACCCTGTTTGCAACTGGAAGAGTTTGCACCGACGAAGCAGGAAATTATCTGTTCCCGCTCGTTTGGTGAACGCATCACGGATTATCCGTCGATGCGGCAGGCCATTTGTAGTTACGCTGCCCGGGCGGCGGAAAAACTTCGCAGCGAGCATCAATATTGTCGGTTTATCTCCACGTTTATTAAGACGTCACCATTTGCGCTCAATGAACCTTATTACGGCAATAGCGCGTCG  >  NC_000913/1231817‑1232112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: