Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,233,154 T→A 10.3% intergenic (‑124/+22) dsbB ← / ← nhaB oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,233,1540TA10.3% 298.5 / 17.5 113intergenic (‑124/+22)dsbB/nhaBoxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (45/55);  new base A (6/6);  total (52/61)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 115 total aligned reads displayed.

GAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGC  >  NC_000913/1233005‑1233300
                                                                                                                                                     |                                                                                                                                                  
 aaCATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCAttt                                                                                                                                                   <  2:1047761/150‑1 (MQ=255)
  aCATTGTTTCAAAAATCGCAACAAAATCATTTCCCTGCAATATGCATAGAGTGCGGAGTTTAAACCAATTCATTCGCTGCGCCACCATGAGGCAGCAAAAATAGGACGCAATTGGACAAATATCGGGCCAAAAGGCAAACCGGGCAATta                                                                                                                                                  <  2:522555/150‑1 (MQ=255)
  aCATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTta                                                                                                                                                  <  2:1287953/150‑1 (MQ=255)
     ttGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTatat                                                                                                                                               <  2:1773275/150‑1 (MQ=255)
     ttGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTatat                                                                                                                                               >  1:386017/1‑150 (MQ=255)
       ggTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGc                                                                                                                                             <  1:1113263/150‑1 (MQ=255)
         ttCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAACCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGCCAATTATCGGGCGAAAAGGCAAACCGGGCAGTTATATGccc                                                                                                                                           >  1:512998/1‑150 (MQ=255)
         ttCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGccc                                                                                                                                           <  1:1596794/150‑1 (MQ=255)
           cAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCgg                                                                                                                                         <  2:15669/150‑1 (MQ=255)
                aTCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCCGCAAAAATAGGACGCAATTGGACAATTATCGGGCTAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgt                                                                                                                                    >  1:499055/1‑150 (MQ=255)
                 tCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGtttgcc                                                                                                                                   <  1:212978/150‑6 (MQ=255)
                 tCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGtttgcc                                                                                                                                   <  1:1468847/150‑6 (MQ=255)
                  cGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttg                                                                                                                                  <  2:1822972/150‑1 (MQ=255)
                  cGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGtttgcct                                                                                                                                  <  1:1786056/150‑7 (MQ=255)
                   gCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttgt                                                                                                                                 >  2:834786/1‑150 (MQ=255)
                    cAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttgtt                                                                                                                                <  1:968220/150‑1 (MQ=255)
                       cATAATAATTTCCATGCAATCTGCATAGAGTGAGCTGATTAAACAAATTCAATCGCTGCGCCACCAGGAGGCAGCAAAAATTCGACGCAATTGGACTATTATCGTGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTGGTTATc                                                                                                                             <  1:893779/150‑1 (MQ=255)
                        aTAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCa                                                                                                                            <  2:812858/150‑1 (MQ=255)
                         tactCATTTCACATCAATATGAATAGAGGGCGCAGTTTAAACCAATTCATTCGCTTCGCCACAATGAGGAAGCAAAAATATTACGAAATTGGACAATTCTCTGGAGAAAAGGCAAACCGGGCAGTTATATGCCCGTTAATTTGTTATCaa                                                                                                                           <  1:759022/147‑1 (MQ=255)
                            tCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGc                                                                                                                        <  2:1090450/150‑1 (MQ=255)
                             cATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCg                                                                                                                       <  1:928702/150‑1 (MQ=255)
                                ttCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTg                                                                                                                    <  2:1723859/150‑1 (MQ=255)
                                   ccTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATTGGACGCAATTGGAAAATAAACGGGCTAAAAGGCAAACCGGGCAGGTATATGCACGGTAAGATGATTACAAAGCGTTGCtt                                                                                                                 >  2:752903/1‑149 (MQ=255)
                                    cTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTAt                                                                                                                <  1:582790/150‑1 (MQ=255)
                                      gCAATATGCATAGAGTGCGCAGTTTAATCAAATTCAATCGCTGCGCCACCAGGTGGCATCATAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCCACCCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATcc                                                                                                              <  1:1170262/150‑1 (MQ=255)
                                      gCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATcc                                                                                                              >  2:1851974/1‑150 (MQ=255)
                                         atatGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGc                                                                                                           >  1:454144/1‑150 (MQ=255)
                                           atGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGGCGCAATTGGACATTTATCGGGCGAAAAGGCAAACCGGGCATTTATTTGCCCGGTATGTGGTTAGCAAAGCGTTGGTATCCTGcgc                                                                                                         >  2:1405904/1‑148 (MQ=255)
                                            tGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCa                                                                                                        >  1:1223009/1‑150 (MQ=255)
                                                tAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGAGAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTg                                                                                                    >  1:1258631/1‑150 (MQ=255)
                                                tAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTg                                                                                                    <  1:500208/150‑1 (MQ=255)
                                                tAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGAAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTg                                                                                                    <  1:1391432/150‑1 (MQ=255)
                                                  tagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGATGCAGCATAAATAGGTCGCAATTGGAAAATTATCGGGCGTAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCa                                                                                                  <  1:1142383/149‑1 (MQ=255)
                                                      gcgcAGTTTAAACCAATTCATTCGCTGCGCCCCCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCAATTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa                                                                                              >  1:1799534/1‑150 (MQ=255)
                                                      gcgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa                                                                                              >  1:564145/1‑150 (MQ=255)
                                                      gcgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa                                                                                              <  1:1557912/150‑1 (MQ=255)
                                                      gcgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa                                                                                              >  2:1870055/1‑150 (MQ=255)
                                                      gcgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTGATCAAAGCGTTGCTATCCAGCCCAGTTGCATaca                                                                                              >  1:1284730/1‑148 (MQ=255)
                                                              tAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCgg                                                                                      >  2:641088/1‑150 (MQ=255)
                                                                 aCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAAATGGACAATTATCGGGCGAAAAGGCAAACCGGGCAGTTATATGCCCGGTAAGTTTTTATCAAAGCGTTGATAGCCAGCCCCTTTGCATAAACCATTCGGGTa                                                                                   >  2:1195912/1‑150 (MQ=255)
                                                                  ccAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTAc                                                                                  <  2:1860981/150‑1 (MQ=255)
                                                                    aaTTCATTCGCTGAGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAg                                                                                <  1:718838/150‑1 (MQ=255)
                                                                       tcattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTGGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAgggg                                                                             <  2:1201382/150‑1 (MQ=255)
                                                                        cattcGCTGCGCCACCAGGGGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTTCATTAACCATTCGGTTACAGTGGc                                                                            >  2:1752499/1‑150 (MQ=255)
                                                                        cattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAACGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGc                                                                            <  1:851325/150‑1 (MQ=255)
                                                                        cattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGc                                                                            >  1:1486037/1‑150 (MQ=255)
                                                                         attcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCa                                                                           >  2:1251647/1‑150 (MQ=255)
                                                                           tcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAg                                                                         >  1:87556/1‑150 (MQ=255)
                                                                             gCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCg                                                                       >  2:1068698/1‑150 (MQ=255)
                                                                             gCTGCGCCACAAGGAGGCAGCTAAAATAAGACGCAATTTGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCg                                                                       <  2:947283/150‑1 (MQ=255)
                                                                                  gcCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTaaag                                                                  >  1:1448656/1‑149 (MQ=255)
                                                                                  gcCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAAc                                                                  <  2:548469/150‑1 (MQ=255)
                                                                                  gcCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAAc                                                                  >  1:1418039/1‑150 (MQ=255)
                                                                                  gcCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAAc                                                                  <  1:1446062/150‑1 (MQ=255)
                                                                                  gcCACCAGGAGGCAGCAAAAATAGGAAGCAAATGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGGAAGTTGTTATCAAAGCGGTGCTATCCAGCCCATGTGCATACACCATTCGGTTACAGGGGCAAGCGTAAAc                                                                  <  1:1288412/150‑1 (MQ=255)
                                                                                   ccaccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACt                                                                 <  2:1436276/150‑1 (MQ=255)
                                                                                    cacccGGAGGCAGCAAAAATAGGACGCAAGTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCACAGCGTTGATATCCAGCCGATTTGCTTAAACCATTCGGTTACAGCGGCAAGCGTAAACta                                                                >  2:1553290/1‑149 (MQ=255)
                                                                                     accaGGAGGCCGCAAAAATAGGACGCAAATGGACAATTAGCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGGTGCTATCCAGCCCATTTGCATAAACCAATCGGTTACAGGGGCAAGCGTAAACTCg                                                               <  2:1242063/150‑1 (MQ=255)
                                                                                        agcaggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCGAAGCGTTGCTATCCAGCCCATTTGCATAACCCATTCGGTTACAGGGGCACGCGTAAACTCGAcc                                                            >  1:292023/4‑149 (MQ=255)
                                                                                          gaggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCa                                                          <  1:1249621/150‑1 (MQ=255)
                                                                                             gcagcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCagag                                                       <  2:1006100/150‑1 (MQ=255)
                                                                                              cagcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGc                                                      >  2:1795082/1‑150 (MQ=255)
                                                                                              cagcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGc                                                      >  1:1747416/1‑150 (MQ=255)
                                                                                              cagcaAAAATAGGACGCAAGTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTAACCAGCCCATTTGCATAAACCAATCGGTTACAGGGGCAAGCGTAAACTCGACGCacagg                                                      >  1:263999/1‑149 (MQ=255)
                                                                                                gcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCaa                                                    >  1:648794/1‑150 (MQ=255)
                                                                                                gcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCaa                                                    >  2:328293/1‑150 (MQ=255)
                                                                                                   aaaaTAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGcc                                                 <  2:861308/150‑1 (MQ=255)
                                                                                                       tAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCTGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgacg                                             <  1:1098713/150‑1 (MQ=255)
                                                                                                       tAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgacg                                             <  2:394591/150‑1 (MQ=255)
                                                                                                        tggACTCAAATGGACAATTATGGGGCGAAAAGGCAAACCGGCCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTCCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgacga                                            <  2:541554/149‑1 (MQ=255)
                                                                                                         ggACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAg                                           <  2:1510856/150‑1 (MQ=255)
                                                                                                           aCGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAgtg                                         <  1:1851974/150‑1 (MQ=255)
                                                                                                           aCGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAgtg                                         <  2:2367/150‑1 (MQ=255)
                                                                                                            cGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGCCGCAGAGCAAGCCGACGAgtgt                                        >  2:1821903/1‑150 (MQ=255)
                                                                                                             gcacatGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCATAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTc                                       <  2:986302/145‑1 (MQ=255)
                                                                                                             gCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTc                                       <  1:1212314/150‑1 (MQ=255)
                                                                                                                aTTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAgg                                    <  1:1402915/150‑1 (MQ=255)
                                                                                                                   ggACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACg                                 <  1:79001/150‑1 (MQ=255)
                                                                                                                     aCAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCTGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAg                               <  2:792643/150‑1 (MQ=255)
                                                                                                                      cAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGATGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAgg                              >  1:1499452/1‑150 (MQ=255)
                                                                                                                        aTTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGgt                            >  1:1653107/1‑150 (MQ=255)
                                                                                                                         ttATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGgtg                           <  2:564145/150‑1 (MQ=255)
                                                                                                                           ttCGGGCGAAAAGGCAAACCGGGCATTTAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCGAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTa                         <  2:1504492/149‑1 (MQ=255)
                                                                                                                           aTCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTa                         <  1:793102/150‑1 (MQ=255)
                                                                                                                             cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAg                       >  2:426401/1‑150 (MQ=255)
                                                                                                                             cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACAAATCGGTTACAGGGGCAATCGTAAACTCGACTCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAg                       >  2:969244/1‑150 (MQ=255)
                                                                                                                              gggCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAgg                      >  1:1018745/1‑150 (MQ=255)
                                                                                                                               ggCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGc                     >  1:464987/1‑150 (MQ=255)
                                                                                                                                 cGAAAAGGCAAACCGGGCATTTATATGCCCAGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAg                   <  1:1815193/150‑1 (MQ=255)
                                                                                                                                    aaaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGTCAGGGc                >  2:1005669/1‑150 (MQ=255)
                                                                                                                                    aaaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGc                >  1:1651142/1‑150 (MQ=255)
                                                                                                                                       ggCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCAt             >  1:19679/1‑150 (MQ=255)
                                                                                                                                       ggCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCAt             >  1:1324251/1‑150 (MQ=255)
                                                                                                                                        gCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATc            >  1:1841579/1‑150 (MQ=255)
                                                                                                                                         cAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATcc           <  1:610851/150‑1 (MQ=255)
                                                                                                                                           taCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGAAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCcac         >  1:68197/2‑150 (MQ=255)
                                                                                                                                            aCCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCCGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGCGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGACATCcacc        >  1:1252553/1‑149 (MQ=255)
                                                                                                                                            aCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCCACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCcaca        >  1:990918/1‑150 (MQ=255)
                                                                                                                                             ccGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGAAAGCGTAAACTCGACTCATAGCAAGCCGACGAGTTTCAGGACGATGGTGTAAGGCAGGGCCATCCacac       >  1:1210332/1‑150 (MQ=255)
                                                                                                                                               gggCATATAAATGCCCGGTAAGTTGTTATCAACGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGGCAGGACGCGGGTGTAAGGCAGGGCCATCCCCCCCa     >  1:1143203/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GAACATTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGC  >  NC_000913/1233005‑1233300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: