Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,233,157 T→A 11.6% intergenic (‑127/+19) dsbB ← / ← nhaB oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,233,1570TA11.6% 287.3 / 19.1 109intergenic (‑127/+19)dsbB/nhaBoxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (45/51);  new base A (6/7);  total (51/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

TTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCA  >  NC_000913/1233010‑1233305
                                                                                                                                                   |                                                                                                                                                    
ttGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTatat                                                                                                                                                    >  1:386017/1‑150 (MQ=255)
ttGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTatat                                                                                                                                                    <  2:1773275/150‑1 (MQ=255)
  ggTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGc                                                                                                                                                  <  1:1113263/150‑1 (MQ=255)
    ttCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAACCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGCCAATTATCGGGCGAAAAGGCAAACCGGGCAGTTATATGccc                                                                                                                                                >  1:512998/1‑150 (MQ=255)
      cAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCgg                                                                                                                                              <  2:15669/150‑1 (MQ=255)
            tCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGtttgcc                                                                                                                                        <  1:212978/150‑6 (MQ=255)
            tCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGtttgcc                                                                                                                                        <  1:1468847/150‑6 (MQ=255)
             cGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttg                                                                                                                                       <  2:1822972/150‑1 (MQ=255)
             cGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATATAAATGCCCGGtttgcct                                                                                                                                       <  1:1786056/150‑7 (MQ=255)
              gCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttgt                                                                                                                                      >  2:834786/1‑150 (MQ=255)
               cAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAgttgtt                                                                                                                                     <  1:968220/150‑1 (MQ=255)
                aaCATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTa                                                                                                                                    <  2:1282286/150‑1 (MQ=255)
                  cATAATAATTTCCATGCAATCTGCATAGAGTGAGCTGATTAAACAAATTCAATCGCTGCGCCACCAGGAGGCAGCAAAAATTCGACGCAATTGGACTATTATCGTGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTGGTTATc                                                                                                                                  <  1:893779/150‑1 (MQ=255)
                   aTAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCa                                                                                                                                 <  2:812858/150‑1 (MQ=255)
                    tactCATTTCACATCAATATGAATAGAGGGCGCAGTTTAAACCAATTCATTCGCTTCGCCACAATGAGGAAGCAAAAATATTACGAAATTGGACAATTCTCTGGAGAAAAGGCAAACCGGGCAGTTATATGCCCGTTAATTTGTTATCaa                                                                                                                                <  1:759022/147‑1 (MQ=255)
                       tCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGc                                                                                                                             <  2:1090450/150‑1 (MQ=255)
                        cATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCg                                                                                                                            <  1:928702/150‑1 (MQ=255)
                           ttCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTg                                                                                                                         <  2:1723859/150‑1 (MQ=255)
                               cTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTAt                                                                                                                     <  1:582790/150‑1 (MQ=255)
                                 gCAATATGCATAGAGTGCGCAGTTTAATCAAATTCAATCGCTGCGCCACCAGGTGGCATCATAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCCACCCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATcc                                                                                                                   <  1:1170262/150‑1 (MQ=255)
                                 gCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATcc                                                                                                                   >  2:1851974/1‑150 (MQ=255)
                                    atatGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGc                                                                                                                >  1:454144/1‑150 (MQ=255)
                                      atGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATGGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGccc                                                                                                              <  2:796718/150‑1 (MQ=255)
                                      atGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGGCGCAATTGGACATTTATCGGGCGAAAAGGCAAACCGGGCATTTATTTGCCCGGTATGTGGTTAGCAAAGCGTTGGTATCCTGcgc                                                                                                              >  2:1405904/1‑148 (MQ=255)
                                        gCATAGAGTGCGCAGTTTTTACCAATTCATTCGCTGCGCTACCAGGATGCAGCATAATTAGTACGCAATTTGACTATTATCGGGCGAAAAGTCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCAt                                                                                                            <  1:1720698/150‑1 (MQ=255)
                                           tAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGAGAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTg                                                                                                         >  1:1258631/1‑150 (MQ=255)
                                           tAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTg                                                                                                         <  1:500208/150‑1 (MQ=255)
                                           tAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGAAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTg                                                                                                         <  1:1391432/150‑1 (MQ=255)
                                             tagTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGATGCAGCATAAATAGGTCGCAATTGGAAAATTATCGGGCGTAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCa                                                                                                       <  1:1142383/149‑1 (MQ=255)
                                                 gcgcAGTTTAAACCAATTCATTCGCTGCGCCCCCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCAATTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa                                                                                                   >  1:1799534/1‑150 (MQ=255)
                                                 gcgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa                                                                                                   <  1:1557912/150‑1 (MQ=255)
                                                 gcgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa                                                                                                   >  2:1870055/1‑150 (MQ=255)
                                                 gcgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATaaa                                                                                                   >  1:564145/1‑150 (MQ=255)
                                                 gcgcAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTGATCAAAGCGTTGCTATCCAGCCCAGTTGCATaca                                                                                                   >  1:1284730/1‑148 (MQ=255)
                                                         tAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCgg                                                                                           >  2:641088/1‑150 (MQ=255)
                                                            aCCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAAATGGACAATTATCGGGCGAAAAGGCAAACCGGGCAGTTATATGCCCGGTAAGTTTTTATCAAAGCGTTGATAGCCAGCCCCTTTGCATAAACCATTCGGGTa                                                                                        >  2:1195912/1‑150 (MQ=255)
                                                             ccAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTAc                                                                                       <  2:1860981/150‑1 (MQ=255)
                                                                  tcattcGCTGCGCCACCAGTAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAgggg                                                                                  <  2:102984/150‑1 (MQ=255)
                                                                  tcattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTGGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAgggg                                                                                  <  2:1201382/150‑1 (MQ=255)
                                                                   cattcGCTGCGCCACCAGGGGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTTCATTAACCATTCGGTTACAGTGGc                                                                                 >  2:1752499/1‑150 (MQ=255)
                                                                   cattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAACGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGc                                                                                 <  1:851325/150‑1 (MQ=255)
                                                                   cattcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGc                                                                                 >  1:1486037/1‑150 (MQ=255)
                                                                      tcGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAg                                                                              >  1:87556/1‑150 (MQ=255)
                                                                       cGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGc                                                                             >  1:1298894/1‑150 (MQ=255)
                                                                        gCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCg                                                                            >  2:1068698/1‑150 (MQ=255)
                                                                        gCTGCGCCACAAGGAGGCAGCTAAAATAAGACGCAATTTGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCg                                                                            <  2:947283/150‑1 (MQ=255)
                                                                             ggcaccaGGAGGCAGAAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAAc                                                                       <  1:1210638/148‑1 (MQ=255)
                                                                             gcCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTaaag                                                                       >  1:1448656/1‑149 (MQ=255)
                                                                             gcCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAAc                                                                       >  1:1418039/1‑150 (MQ=255)
                                                                             gcCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAAc                                                                       <  2:548469/150‑1 (MQ=255)
                                                                             gcCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAAc                                                                       <  1:1446062/150‑1 (MQ=255)
                                                                             gcCACCAGGAGGCAGCAAAAATAGGAAGCAAATGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGGAAGTTGTTATCAAAGCGGTGCTATCCAGCCCATGTGCATACACCATTCGGTTACAGGGGCAAGCGTAAAc                                                                       <  1:1288412/150‑1 (MQ=255)
                                                                              ccaccaGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACt                                                                      <  2:1436276/150‑1 (MQ=255)
                                                                               cacccGGAGGCAGCAAAAATAGGACGCAAGTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCACAGCGTTGATATCCAGCCGATTTGCTTAAACCATTCGGTTACAGCGGCAAGCGTAAACta                                                                     >  2:1553290/1‑149 (MQ=255)
                                                                                accaGGAGGCCGCAAAAATAGGACGCAAATGGACAATTAGCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGGTGCTATCCAGCCCATTTGCATAAACCAATCGGTTACAGGGGCAAGCGTAAACTCg                                                                    <  2:1242063/150‑1 (MQ=255)
                                                                                   agcaggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCGAAGCGTTGCTATCCAGCCCATTTGCATAACCCATTCGGTTACAGGGGCACGCGTAAACTCGAcc                                                                 >  1:292023/4‑149 (MQ=255)
                                                                                     gaggCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCa                                                               <  1:1249621/150‑1 (MQ=255)
                                                                                     cagccagaaAAAATACGACCCAATGTCACAATAATCGGGCGAAAACGCAAACCGGGCATATATAAGCCCGGTAAGTTGTTATCAACGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGCTACAGGGGCAAGCGTAAACTCGACGCa                                                               <  1:893013/146‑1 (MQ=255)
                                                                                       ggCAGCAAAAATAGGACGCAATTGGACAATTATCTGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCaga                                                             <  1:1451756/150‑1 (MQ=255)
                                                                                        gcagcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCagag                                                            <  2:1006100/150‑1 (MQ=255)
                                                                                         cagcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGc                                                           >  1:1747416/1‑150 (MQ=255)
                                                                                         cagcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGc                                                           >  2:1795082/1‑150 (MQ=255)
                                                                                         cagcaAAAATAGGACGCAAGTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTAACCAGCCCATTTGCATAAACCAATCGGTTACAGGGGCAAGCGTAAACTCGACGCacagg                                                           >  1:263999/1‑149 (MQ=255)
                                                                                           gcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCaa                                                         >  2:328293/1‑150 (MQ=255)
                                                                                           gcaAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCaa                                                         >  1:648794/1‑150 (MQ=255)
                                                                                              aaaaTAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGcc                                                      <  2:861308/150‑1 (MQ=255)
                                                                                                  tAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCTGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgacg                                                  <  1:1098713/150‑1 (MQ=255)
                                                                                                  tAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgacg                                                  <  2:394591/150‑1 (MQ=255)
                                                                                                   tggACTCAAATGGACAATTATGGGGCGAAAAGGCAAACCGGCCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTCCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCcgacga                                                 <  2:541554/149‑1 (MQ=255)
                                                                                                    ggACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAg                                                <  2:1510856/150‑1 (MQ=255)
                                                                                                      aCGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAgtg                                              <  1:1851974/150‑1 (MQ=255)
                                                                                                       cGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGCCGCAGAGCAAGCCGACGAgtgt                                             >  2:1821903/1‑150 (MQ=255)
                                                                                                        gcacatGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCATAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTc                                            <  2:986302/145‑1 (MQ=255)
                                                                                                        gCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTc                                            <  1:1212314/150‑1 (MQ=255)
                                                                                                              ggACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACg                                      <  1:79001/150‑1 (MQ=255)
                                                                                                                aCAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCTGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAg                                    <  2:792643/150‑1 (MQ=255)
                                                                                                                 cAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGATGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAgg                                   >  1:1499452/1‑150 (MQ=255)
                                                                                                                   aTTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGgt                                 >  1:1653107/1‑150 (MQ=255)
                                                                                                                    ttATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGgtg                                <  2:564145/150‑1 (MQ=255)
                                                                                                                      ttCGGGCGAAAAGGCAAACCGGGCATTTAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCGAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTa                              <  2:1504492/149‑1 (MQ=255)
                                                                                                                      aTCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTa                              <  1:793102/150‑1 (MQ=255)
                                                                                                                        cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAg                            >  2:426401/1‑150 (MQ=255)
                                                                                                                        cGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACAAATCGGTTACAGGGGCAATCGTAAACTCGACTCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAg                            >  2:969244/1‑150 (MQ=255)
                                                                                                                         gggCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAgg                           >  1:1018745/1‑150 (MQ=255)
                                                                                                                          ggCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGc                          >  1:464987/1‑150 (MQ=255)
                                                                                                                            cGAAAAGGCAAACCGGGCATTTATATGCCCAGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAg                        <  1:1815193/150‑1 (MQ=255)
                                                                                                                               aaaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGTCAGGGc                     >  2:1005669/1‑150 (MQ=255)
                                                                                                                               aaaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGc                     >  1:1651142/1‑150 (MQ=255)
                                                                                                                               aaaGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGc                     >  1:211551/1‑150 (MQ=255)
                                                                                                                                acaacttaCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGGAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTCAGGCAGGTcc                    >  2:44834/8‑150 (MQ=255)
                                                                                                                                  ggCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCAt                  >  1:1324251/1‑150 (MQ=255)
                                                                                                                                   gCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATc                 >  1:1841579/1‑150 (MQ=255)
                                                                                                                                   acttaCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCGATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATc                 >  1:482893/5‑150 (MQ=255)
                                                                                                                                    cAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATcc                <  1:610851/150‑1 (MQ=255)
                                                                                                                                      taCCGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGAAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCcac              >  1:68197/2‑150 (MQ=255)
                                                                                                                                       aCCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCCGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGCGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGACATCcacc             >  1:1252553/1‑149 (MQ=255)
                                                                                                                                        ccGGGCATATAAATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGAAAGCGTAAACTCGACTCATAGCAAGCCGACGAGTTTCAGGACGATGGTGTAAGGCAGGGCCATCCacac            >  1:1210332/1‑150 (MQ=255)
                                                                                                                                          gggCATATAAATGCCCGGTAAGTTGTTATCAACGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGGCAGGACGCGGGTGTAAGGCAGGGCCATCCCCCCCa          >  1:1143203/1‑150 (MQ=255)
                                                                                                                                             cATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTAAAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGCTGTAAGGCAGGGCCATCCACACCATGc       >  1:65254/1‑150 (MQ=255)
                                                                                                                                                  atatGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTAGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGCCGAGGGTGTCAGGCAGGGCCATCCACACCATGCGGCCa  >  2:1345936/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTGGTTCAAAAATCGCAACATAATCATTTCCCTGCAATATGCATAGAGTGCGCAGTTTAAACCAATTCATTCGCTGCGCCACCAGGAGGCAGCAAAAATAGGACGCAATTGGACAATTATCGGGCGAAAAGGCAAACCGGGCATTTATATGCCCGGTAAGTTGTTATCAAAGCGTTGCTATCCAGCCCATTTGCATAAACCATTCGGTTACAGGGGCAAGCGTAAACTCGACGCAGAGCAAGCCGACGAGTGTCAGGACGAGGGTGTAAGGCAGGGCCATCCACACCATGCGGCCA  >  NC_000913/1233010‑1233305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: