Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,235,687 A→T 5.2% intergenic (+30/+22) fadR → / ← ycgB fatty acid metabolism regulon transcriptional regulator/SpoVR family stationary phase protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,235,6870AT 5.2% 344.5 / 4.6 121intergenic (+30/+22)fadR/ycgBfatty acid metabolism regulon transcriptional regulator/SpoVR family stationary phase protein
Reads supporting (aligned to +/- strand):  ref base A (58/56);  new base T (4/3);  total (62/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

Only 100 of 121 total aligned reads displayed.

GAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCC  >  NC_000913/1235541‑1235833
                                                                                                                                                  |                                                                                                                                                  
gAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACg                                                                                                                                                 >  2:1463021/1‑150 (MQ=255)
  aGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAg                                                                                                                                               <  1:185264/150‑1 (MQ=255)
  aGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAg                                                                                                                                               <  1:1362434/150‑1 (MQ=255)
     cgcgcACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAgggg                                                                                                                                            >  2:1054199/1‑150 (MQ=255)
       cgcACGATCAGGTGTACGAAACAGTGTGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGtt                                                                                                                                          >  2:952759/1‑150 (MQ=255)
         cACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCGTTTGAGGGGtttg                                                                                                                                        <  1:1116607/150‑2 (MQ=255)
         cACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGtttt                                                                                                                                        >  1:1233828/1‑150 (MQ=255)
          aCGATCCGGTGTACGAAACAGGGCGTCGCTATGGGCATGAGTGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCCTTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGttttt                                                                                                                                       <  1:100321/150‑1 (MQ=255)
          aCGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCGTTTGAGGGGtttgc                                                                                                                                       <  1:1646219/150‑3 (MQ=255)
          aCGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGttttt                                                                                                                                       <  2:957562/150‑1 (MQ=255)
           cGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGttgttt                                                                                                                                      >  2:763042/1‑150 (MQ=255)
             aTCAGGGGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTttgt                                                                                                                                    <  2:1395876/150‑1 (MQ=255)
                aGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTtgttgt                                                                                                                                 <  1:1450325/150‑1 (MQ=255)
                      aCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTaa                                                                                                                           >  1:463427/1‑149 (MQ=255)
                      aCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTAc                                                                                                                           >  1:1142061/1‑150 (MQ=255)
                      aCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTAc                                                                                                                           >  1:587330/1‑150 (MQ=255)
                      aCGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTAc                                                                                                                           >  2:369198/1‑150 (MQ=255)
                      aCGAAACAGTGCGGCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTAc                                                                                                                           <  1:1554925/150‑1 (MQ=255)
                       cGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACa                                                                                                                          >  1:1583112/1‑150 (MQ=255)
                              gTGGGTCGCTATGGGCATGAGTGTGGCGAGTTTTGGCACCGGATGATGAAAAATGTGCCGGGTTATTTAGCTATTCTTGGGCGATATTCCCTTCCGTTTAAATAGCAAACCCCTCAAACTAGGGGTTTTTTGTTGTTTTTACAGATTTcc                                                                                                                   <  2:364601/150‑1 (MQ=255)
                               tGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTccc                                                                                                                  <  1:1101589/150‑1 (MQ=255)
                                gCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCa                                                                                                                 >  2:1090877/1‑150 (MQ=255)
                                   gcGCTATGGGCATGAGAGTGGCGAGAGCTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTc                                                                                                              <  1:1029392/149‑1 (MQ=255)
                                     gCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCtt                                                                                                            >  1:1067866/1‑150 (MQ=255)
                                      cTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATATGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTg                                                                                                           <  2:1220243/150‑1 (MQ=255)
                                       tATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTgg                                                                                                          <  1:1261988/150‑1 (MQ=255)
                                            gCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGc                                                                                                     <  2:812069/150‑1 (MQ=255)
                                              aTGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCATACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCaa                                                                                                   >  2:555198/1‑150 (MQ=255)
                                                gagaGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACg                                                                                                 <  2:587330/150‑1 (MQ=255)
                                                  gagTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGtt                                                                                               <  2:918940/150‑1 (MQ=255)
                                                  gagTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGtt                                                                                               <  2:1465312/150‑1 (MQ=255)
                                                      ggCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCGATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAACCGAGGGGGTTTTTTGTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAg                                                                                           >  1:328522/1‑150 (MQ=255)
                                                      ggCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCTATAATCCCTTCCGTTTATCGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTGTTGGCGGGCAACGTTCCAg                                                                                           <  2:349095/150‑1 (MQ=255)
                                                      ggCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAg                                                                                           >  2:1812438/1‑150 (MQ=255)
                                                      ggCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAg                                                                                           <  2:512673/150‑1 (MQ=255)
                                                       gCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGc                                                                                          <  2:1528418/150‑1 (MQ=255)
                                                        cGAGATTTGGCCCCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGACCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGcc                                                                                         >  1:485184/1‑149 (MQ=255)
                                                         gagaTTTGTCACCGGATGCAGAAAAATCTGCCGTGTGATTAAGACATTCAGGGGCGATAATCCCTTCCGTTTAAACAGCAAACCCCTCAAACGAGTGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCaa                                                                                        <  2:306407/150‑1 (MQ=255)
                                                         gagaTTTGGCACCGGAGGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGGGATAATCCCTTCCGTTAAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCaa                                                                                        <  2:306406/150‑1 (MQ=255)
                                                          agaTTTGGCACCGGATGAAGAAAAATCTGCAGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCATACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGAAACGTTCCAGCAAc                                                                                       <  1:276476/150‑1 (MQ=255)
                                                                ggCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGAtg                                                                                 >  2:1773354/1‑150 (MQ=255)
                                                                 gCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTATTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGAtgc                                                                                >  2:1267204/1‑150 (MQ=255)
                                                                     cGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGcc                                                                            >  2:1495622/1‑150 (MQ=255)
                                                                        aTGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGATTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTc                                                                         <  2:64398/150‑1 (MQ=255)
                                                                           cAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTc                                                                      >  1:859828/1‑150 (MQ=255)
                                                                           cAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTc                                                                      >  1:45772/1‑150 (MQ=255)
                                                                            aGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCg                                                                     >  1:705974/1‑150 (MQ=255)
                                                                             gAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGt                                                                    >  1:1615221/1‑150 (MQ=255)
                                                                              aaaaaTCTGCCGGGTGATTTAGCCATTCATGGGCGATAATCCCTTCCGTTTAAAGAGCAACCCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCAATCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGtt                                                                   <  2:352197/150‑1 (MQ=255)
                                                                                aaaTCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCTATGCTGCCGTCTTCGtttt                                                                 >  1:181507/1‑150 (MQ=255)
                                                                                aaaTCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTGTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGGTTTTACAGATTTCCCATTCTTGGCGGGCATCGGTCCAGCAACTCGATGCTGCCGTCTTCGtttt                                                                 <  2:264577/150‑1 (MQ=255)
                                                                                 aaTCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTg                                                                <  1:910912/150‑1 (MQ=255)
                                                                                  aTCTGCCGTGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGc                                                               <  2:383491/150‑1 (MQ=255)
                                                                                  aTCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGc                                                               <  1:369198/150‑1 (MQ=255)
                                                                                  aTCTGCCGGGAGATGTAGCCATTCAGGGGCGATAATCCCTTCCGTTTACAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGc                                                               <  1:1120710/150‑1 (MQ=255)
                                                                                   tCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCt                                                              <  2:55434/150‑1 (MQ=255)
                                                                                   tCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCt                                                              <  2:1652098/150‑1 (MQ=255)
                                                                                   tCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCt                                                              <  2:399632/150‑1 (MQ=255)
                                                                                    cTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTg                                                             >  1:752534/1‑150 (MQ=255)
                                                                                    cTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTg                                                             >  2:1857792/1‑150 (MQ=255)
                                                                                        cGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCg                                                         <  2:400764/150‑1 (MQ=255)
                                                                                         gggTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGa                                                        >  1:1760423/1‑150 (MQ=255)
                                                                                            tGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGca                                                     <  1:118929/150‑1 (MQ=255)
                                                                                                ttAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGcatcac                                                 <  2:1873144/150‑1 (MQ=255)
                                                                                                 tAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGCGcatcaca                                                >  1:1844911/1‑150 (MQ=255)
                                                                                                  aGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGcatcacat                                               >  2:783508/1‑150 (MQ=255)
                                                                                                    ccATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGcatcacatca                                             <  1:441358/150‑1 (MQ=255)
                                                                                                    ccATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGcatcacatca                                             <  1:546721/150‑1 (MQ=255)
                                                                                                     cATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCaa                                            >  2:1603340/1‑150 (MQ=255)
                                                                                                     cATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCaa                                            >  2:312431/1‑150 (MQ=255)
                                                                                                     cATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACACGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCaa                                            >  2:458191/1‑150 (MQ=255)
                                                                                                        tCAGGGGCGATATTCCATTTCGTTTAAAGAGCAATCCCCTCAATCTAGGGGTTTTTTGTTGTTTTTAGAGTTTTCCCATTCTTGGCGGGCAACGTTACAGCAACTCGATGCGGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATc                                         <  1:40791/150‑1 (MQ=255)
                                                                                                         cAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATcc                                        <  1:952759/150‑1 (MQ=255)
                                                                                                          aGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATccc                                       <  1:1463021/150‑1 (MQ=255)
                                                                                                           ggggCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATcccc                                      <  2:1659855/150‑1 (MQ=255)
                                                                                                           ggggCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATcccc                                      >  2:1283082/1‑150 (MQ=255)
                                                                                                            gggCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCca                                     >  2:588481/1‑150 (MQ=255)
                                                                                                                gATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAgg                                 <  2:1165965/150‑1 (MQ=255)
                                                                                                                   aaacccTTCAGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTCTTGTTTTTACAGATTTCCCATACTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCCTCTTCGTTTTGCTGTTCTAGCATCACAACAAATCACCACAGCCGa                              >  2:769244/4‑150 (MQ=255)
                                                                                                                    aTCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGAt                             >  2:227313/1‑150 (MQ=255)
                                                                                                                       ccTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGca                          >  2:631194/1‑150 (MQ=255)
                                                                                                                            cGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTAGAGATATCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGc                     <  2:1365962/150‑1 (MQ=255)
                                                                                                                             gTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACCTCAAATCCCCACAGGCGATGCACATGCt                    >  1:1510016/1‑150 (MQ=255)
                                                                                                                             gTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCt                    >  1:607876/1‑150 (MQ=255)
                                                                                                                                  aacaaaaaaCCCCTCGTTTGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCCCAGGCGATGCACATGCTTCAgt               >  2:795672/7‑149 (MQ=255)
                                                                                                                                  aaGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAgg               <  1:1426016/150‑1 (MQ=255)
                                                                                                                                  aaGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAgg               >  2:622690/1‑150 (MQ=255)
                                                                                                                                  aaGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAgg               <  1:1054199/150‑1 (MQ=255)
                                                                                                                                   agagCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACTTGCTTCAggc              >  2:913650/1‑149 (MQ=255)
                                                                                                                                   agagCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAggg              >  2:401017/1‑149 (MQ=255)
                                                                                                                                    gagCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGAc             >  2:465715/1‑150 (MQ=255)
                                                                                                                                    gagCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGAc             >  1:1396421/1‑150 (MQ=255)
                                                                                                                                      gCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACCTCAAATCCCCACAGGCGATGCACATGCTTCAGGActt           >  1:742987/1‑150 (MQ=255)
                                                                                                                                       cAAACCCCTCAACCGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTGCCAGCAACTCGATGCTGCCGGCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGActtc          <  2:1810964/150‑1 (MQ=255)
                                                                                                                                       aaaaCCCCTCGTTTGAGGGGTTTTTTGTTGTTTTTACAGATTTCACATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGCGCATCACATCAAATCCCCACAGGCGCTGCACATGCTTCAGGActtc          >  2:15019/2‑150 (MQ=255)
                                                                                                                                         aaCCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGActtctt        <  1:1447117/150‑1 (MQ=255)
                                                                                                                                         aaCCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCATCGTTTTGCTGTTCCAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGActtatt        >  2:1790741/1‑150 (MQ=255)
                                                                                                                                           ccccTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTg      >  1:1003169/1‑150 (MQ=255)
                                                                                                                                              cTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTgcgc   >  2:346482/1‑150 (MQ=255)
                                                                                                                                               tCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGcc  <  1:377067/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GAAGGCGCGCACGATCAGGTGTACGAAACAGTGCGTCGCTATGGGCATGAGAGTGGCGAGATTTGGCACCGGATGCAGAAAAATCTGCCGGGTGATTTAGCCATTCAGGGGCGATAATCCCTTCCGTTTAAAGAGCAAACCCCTCAAACGAGGGGTTTTTTGTTGTTTTTACAGATTTCCCATTCTTGGCGGGCAACGTTCCAGCAACTCGATGCTGCCGTCTTCGTTTTGCTGTTCGAGCATCACATCAAATCCCCACAGGCGATGCACATGCTTCAGGACTTCTTTGCGCC  >  NC_000913/1235541‑1235833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: