Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,279,508 T→C 5.1% intergenic (+160/‑356) narK → / → narG nitrate/nitrite transporter/nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,279,5080TC 5.1% 353.5 / 6.8 120intergenic (+160/‑356)narK/narGnitrate/nitrite transporter/nitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (56/55);  new base C (2/4);  total (60/60)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.81e-01

Only 100 of 122 total aligned reads displayed.

GTCATACTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGCATAAAAATCTTAATAGTTTAAATAACTA  >  NC_000913/1279359‑1279657
                                                                                                                                                      |                                                                                                                                                     
gTCATACTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGt                                                                                                                                                       <  2:884989/150‑1 (MQ=255)
    tACTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATTGAGTAAAATTTTTGATTATCCTTTGCGCGGCATGATGTcgcg                                                                                                                                                   >  1:449725/1‑150 (MQ=255)
    tACTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTcgcg                                                                                                                                                   <  2:427754/150‑1 (MQ=255)
      cTTCGGGTTACATGTGCTGCGGCTGCGTTAATTAACCCCACTCACTTAAGTTAGTTAGCGCCGGGGATTGATTAACTTGGCGCCGTCCTGTTAACCGAATTTTATATAGTTAAAAATTTTTATTTTCATTTCGCGGGATGATGTCgcgcg                                                                                                                                                 >  2:1395008/1‑149 (MQ=255)
       ttCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTGCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGAGTTTGCTTTGCGCGGCAGGATGTCgcgcgt                                                                                                                                                >  1:914510/1‑148 (MQ=255)
        tCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGACATCATGCCgcgcaaa                                                                                                                                               <  1:1015110/150‑4 (MQ=255)
        tCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGACATCATGCCgcgcaaa                                                                                                                                               <  1:744154/150‑4 (MQ=255)
          gggTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATTTAGAGTAATATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCttttt                                                                                                                                             >  1:145388/1‑150 (MQ=255)
          gggTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCttttt                                                                                                                                             <  2:1559909/150‑1 (MQ=255)
          gggTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGATATCATGCCgcgcaaagg                                                                                                                                             <  2:349833/150‑6 (MQ=37)
            gTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCttttttt                                                                                                                                           <  2:452725/150‑1 (MQ=255)
              tACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTAt                                                                                                                                         <  1:1332697/150‑1 (MQ=255)
               aCATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATg                                                                                                                                        <  1:864125/150‑1 (MQ=255)
               aCATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATg                                                                                                                                        <  2:1800942/150‑1 (MQ=255)
                cATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGc                                                                                                                                       >  1:1225865/1‑150 (MQ=255)
                 aTGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCg                                                                                                                                      >  2:1464974/1‑150 (MQ=255)
                 aTGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCg                                                                                                                                      <  2:1247398/150‑1 (MQ=255)
                  tgtgCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTGCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGt                                                                                                                                     >  1:273556/1‑150 (MQ=255)
                  tgtgCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTTATTATCCTTTGCGCGGCATGATGTAGCGCTTTTTTTATGCGt                                                                                                                                     >  2:1166054/1‑150 (MQ=255)
                  tgtgCTGCGGCTGCGTTAATTCACCCCAGTCACTTTCTTTAGTAAGCTCCTGGGATTCATTCACTTGGCGCCTTCCGGTAAAACGAATTATATAGAGTAAAAAATTTGATTATGCTTTGCGCGGCATGATGTCGCGCTTTTTTTTTgggg                                                                                                                                     >  1:1184718/1‑147 (MQ=255)
                    tgctgcGGCTGCGTTCATTCACCCCAGTCCCTTACTTTAGTAAGCTCCTGGGATTCAATCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTATTTATGAGTCa                                                                                                                                   >  2:1134051/1‑150 (MQ=255)
                     gctgcggctgcgTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCAt                                                                                                                                  >  1:435137/1‑150 (MQ=255)
                       tgcggctgcgTTCATTCACCCCAGTCAATTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATTTAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCAttt                                                                                                                                <  1:1148563/150‑1 (MQ=255)
                         cggccgcgTGCATTCACCCCAGTCACTTCCTTTAGTAAGCTCCTGGGATTCATTCACTTTCCGCCTTCCTGTAAACCGCATTATATAGAGGAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAg                                                                                                                              <  2:1151678/150‑1 (MQ=255)
                          ggctgcgTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGt                                                                                                                             <  2:568780/150‑1 (MQ=255)
                          ggctgcgTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGt                                                                                                                             <  2:1504531/150‑1 (MQ=255)
                            ctgcgTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTa                                                                                                                           >  2:436184/1‑150 (MQ=255)
                             tgcgTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTac                                                                                                                          <  1:1535945/150‑1 (MQ=255)
                             tgcgTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTac                                                                                                                          >  2:398262/1‑150 (MQ=255)
                               cgTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTacaa                                                                                                                        >  2:1127813/1‑150 (MQ=255)
                               cgTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGACATCATGCCgcgcaaa                                                                                                                                               >  2:1489865/1‑124 (MQ=255)
                               cgTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGACATCATGCCgcgcaaa                                                                                                                                               <  1:1489865/127‑4 (MQ=255)
                                 ttcattcaCCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATCTTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTacaaca                                                                                                                      <  2:263582/150‑1 (MQ=255)
                                  tcattcaCCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACAt                                                                                                                     <  1:1539790/150‑1 (MQ=255)
                                    attcaCCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATAc                                                                                                                   >  1:1408628/1‑150 (MQ=255)
                                    attcaCCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGACGCGCTTTTTTTATGAGTCAATTAGTTACAACATaa                                                                                                                   >  1:165559/1‑149 (MQ=255)
                                     ttcaCCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACt                                                                                                                  <  2:889438/150‑1 (MQ=255)
                                        aCCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAAt                                                                                                               >  1:163520/1‑150 (MQ=255)
                                         ccccATTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATg                                                                                                              <  1:1769083/150‑1 (MQ=255)
                                         ccccAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATg                                                                                                              <  2:1559274/150‑1 (MQ=255)
                                          cccATTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCCTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGt                                                                                                             <  1:344445/150‑1 (MQ=255)
                                           ccAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGtt                                                                                                            <  2:1548996/150‑1 (MQ=255)
                                                cACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATg                                                                                                       <  1:1051271/150‑1 (MQ=255)
                                                    tacttTAGTGAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGttt                                                                                                   >  2:509529/1‑150 (MQ=255)
                                                      cttTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTAt                                                                                                 >  1:393370/1‑150 (MQ=255)
                                                        tcaGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTAttt                                                                                               >  1:1511331/3‑150 (MQ=255)
                                                               gCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGa                                                                                        <  1:55184/150‑1 (MQ=255)
                                                                   cTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTc                                                                                    <  1:1769517/150‑1 (MQ=255)
                                                                      ggATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCAtt                                                                                 <  2:436318/150‑1 (MQ=255)
                                                                       gATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTa                                                                                >  2:743468/1‑150 (MQ=255)
                                                                          tcattcaCTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAaga                                                                             <  1:1805092/150‑1 (MQ=255)
                                                                           cattcaCTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAagag                                                                            >  1:142787/1‑150 (MQ=255)
                                                                            attcaCTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGcc                                                                          >  1:187417/1‑150 (MQ=255)
                                                                             ttcaCTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGcc                                                                          >  2:932302/1‑150 (MQ=255)
                                                                                    gccgccTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAata                                                                   >  2:593301/1‑150 (MQ=255)
                                                                                            ccTGTAAACCGAATTATATATAGTAAAATATTTGATTATCCATTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTAAATCGCCGGATTTCATTAAGAGCCATTAATATGTTAccc                                                           <  1:1494379/150‑1 (MQ=255)
                                                                                             cTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCa                                                          >  2:1093556/1‑150 (MQ=255)
                                                                                             cTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCa                                                          >  1:90719/1‑150 (MQ=255)
                                                                                             cTGTAAAACGAATTATATAGAGTAAAATATTTGAGTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTAAGTTACAACATACTAATGTTATATCGTTTATTTAGCCGGATTTCATTAACAGCCATTAATATGTTAcaca                                                          >  1:90722/1‑150 (MQ=255)
                                                                                              tGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCAt                                                         >  1:277787/1‑150 (MQ=255)
                                                                                               gTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTAACCAtt                                                        >  1:1326703/1‑149 (MQ=255)
                                                                                               gTAAACCGAATTAAATAGAGTAAAATATTTGATTATCCATTGCGCGCCATGATGTCGCGCTTTATTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCTGATATCATTAATAGCCATTAATATGTTACCCATg                                                        >  1:1126341/1‑150 (MQ=255)
                                                                                                 aaaCCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATTTTACCCATggg                                                      >  2:1314852/1‑150 (MQ=255)
                                                                                                  aaCAGAATTATATACAGTAAAATATTTGATTATCCTTTGCGCGGCATGCTGTCGCGCTTTTTTTATGCGTCATTTAGTTACAAAATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATgggg                                                     <  2:958391/150‑1 (MQ=255)
                                                                                                   aCCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTATTATGTTACCCATGGGGa                                                    <  1:993703/150‑1 (MQ=255)
                                                                                                    ccGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGaa                                                   >  1:1088254/1‑150 (MQ=255)
                                                                                                    ccGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGaa                                                   >  1:551080/1‑150 (MQ=255)
                                                                                                    ccGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGaa                                                   >  2:772270/1‑150 (MQ=255)
                                                                                                      gAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGATTa                                                 >  2:450441/1‑150 (MQ=255)
                                                                                                      gAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATa                                                 >  1:1316832/1‑150 (MQ=255)
                                                                                                      gAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACGAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGtata                                                 >  2:343045/1‑150 (MQ=255)
                                                                                                      gAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGCCATGATGACGCCCTATATAAATCCGACATATACTTACAACAAACAAATCATATATGGATTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATa                                                 >  1:1805127/1‑150 (MQ=255)
                                                                                                        aTTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACt                                               <  2:1547050/150‑1 (MQ=255)
                                                                                                         ttATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTc                                              <  2:77895/150‑1 (MQ=255)
                                                                                                          tatataGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTcc                                             <  2:1474355/150‑1 (MQ=255)
                                                                                                            tataGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCtt                                           <  1:1627254/150‑1 (MQ=255)
                                                                                                            tataGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCtt                                           <  2:399067/150‑1 (MQ=255)
                                                                                                             ataGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTa                                          <  1:398262/150‑1 (MQ=255)
                                                                                                               agagTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCTTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAAt                                        <  2:833162/150‑1 (MQ=255)
                                                                                                               agagTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAAt                                        <  2:787805/150‑1 (MQ=255)
                                                                                                                gagTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATa                                       >  2:554835/1‑150 (MQ=255)
                                                                                                                  gTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCTCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCTCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGTGAATAATCCTTAATAcc                                     >  1:202080/1‑150 (MQ=255)
                                                                                                                  gTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTTCAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATAcc                                     >  2:509763/1‑150 (MQ=255)
                                                                                                                         tatTTGATTATCCTTTGCGCGGCATGAAGTCGCGCTTTTTTTATGCGGCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGTTTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTg                               <  2:925526/150‑1 (MQ=255)
                                                                                                                          atTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGc                              >  2:507470/1‑150 (MQ=255)
                                                                                                                          atTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGc                              <  2:1511331/150‑1 (MQ=255)
                                                                                                                                 tATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGCATaaaaa                       <  1:1642456/150‑1 (MQ=255)
                                                                                                                                    ccTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGCATAAAAATCt                    <  2:949596/150‑1 (MQ=255)
                                                                                                                                     cTTTGCGCGGCATGCTGGCGCGCTTTTTTTATGCGTCATTTAGGTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGCATAAAAATCtt                   <  2:163520/150‑1 (MQ=255)
                                                                                                                                      tttGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGCATAAAAATCTTa                  <  2:234569/150‑1 (MQ=255)
                                                                                                                                       ttGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGCATAAAAATCTTaa                 <  1:1437115/150‑1 (MQ=255)
                                                                                                                                         gcgcGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGCATAAAAATCTTAATa               <  1:36432/150‑1 (MQ=255)
                                                                                                                                          cgcgGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCCTCTGCATAAAAATCTTAATAg              >  2:267703/1‑150 (MQ=255)
                                                                                                                                          cgcgGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGCATAAAAATCTTAATAg              <  1:1806408/150‑1 (MQ=255)
                                                                                                                                          cgcgGCATGATGTCGCGCTTTTTTTATGCGTCAGTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAATAGCCAATAATATGTTACCCATGGGGATTACTCCTTAATACCCATCTGCATAAAATTCTTAATAg              >  2:136327/1‑150 (MQ=255)
                                                                                                                                            cgGCATGATTTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGTATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGCATAAAAATCTTAATAGtt            <  1:555680/150‑1 (MQ=255)
                                                                                                                                              acatcatGCCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGCATAAAAATCTTAATAGTTTa          >  2:533673/2‑150 (MQ=255)
                                                                                                                                                 tgatgTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGCATAAAAATCTTAATAGTTTAAAt       >  1:953444/1‑150 (MQ=255)
                                                                                                                                                    tgTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGCATAAAAATCTTAATAGTTTAAATAAc    <  2:1511617/150‑1 (MQ=255)
                                                                                                                                                      tCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGTTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGCATAAAAATCTTAATAGTTTAAATAACTa  <  1:986060/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GTCATACTTCGGGTTACATGTGCTGCGGCTGCGTTCATTCACCCCAGTCACTTACTTTAGTAAGCTCCTGGGATTCATTCACTTGCCGCCTTCCTGTAAACCGAATTATATAGAGTAAAATATTTGATTATCCTTTGCGCGGCATGATGTCGCGCTTTTTTTATGCGTCATTTAGTTACAACATACTAATGTTATATGGTTTATTTCGCCGGATTTCATTAAGAGCCATTAATATGTTACCCATGGGGAATACTCCTTAATACCCATCTGCATAAAAATCTTAATAGTTTAAATAACTA  >  NC_000913/1279359‑1279657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: