| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,287,009 | A→T | 17.2% | intergenic (+483/+57) | narI → / ← rttR | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,287,009 | 0 | A | T | 17.2% | 77.5 / 4.9 | 43 | intergenic (+483/+57) | narI/rttR | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript |
| Reads supporting (aligned to +/- strand): ref base A (17/18); new base T (3/5); total (20/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAG > NC_000913/1286861‑1287151 | tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAt > 2:173789/1‑150 (MQ=255) gaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg > 2:1002228/1‑150 (MQ=255) ggaaGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGc > 2:955322/3‑150 (MQ=255) gtggtggGTGAATGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCtt < 2:891849/150‑1 (MQ=255) gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgag < 1:1853134/150‑1 (MQ=255) ggggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGGCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgaga < 2:150706/149‑1 (MQ=255) gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGagag < 1:370553/150‑1 (MQ=255) gggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGATTGagag > 2:776676/1‑150 (MQ=255) gggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGGCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGGGGATTCGGTTGAGAGtt > 1:1229393/1‑150 (MQ=255) aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg < 1:637921/150‑1 (MQ=255) gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa < 1:173789/150‑1 (MQ=255) gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCTAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt < 1:1204488/150‑1 (MQ=255) gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGG < 2:634038‑M1/150‑1 (MQ=255) gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTG < 1:955322‑M1/150‑1 (MQ=255) cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGACCCTTAGTCGCAGCTTCTCATCCTTACCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGG > 1:1772141‑M1/1‑150 (MQ=255) cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCTAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGG < 1:1708428‑M1/150‑1 (MQ=255) ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGTCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGG < 2:910513‑M1/150‑1 (MQ=255) tCGGGTCGTTGCCTGCGGCAACGCTCTCTAGCTGACGCTCGAGCCGAACCGTAGTCGAAGCATATCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGCTTGGGAGTTCAGGGACGTTTGAAAGTGATGGTGGTTGGGGAAg > 2:571124/1‑150 (MQ=14) tCGGGTCGTTACCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGATTGTGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAG > 1:1262777‑M1/1‑150 (MQ=255) cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCTAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGG < 2:406088‑M1/150‑1 (MQ=255) gTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGACGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGATTGAAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATT > 2:1014916‑M1/1‑150 (MQ=255) tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGTGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTa > 2:1115873/1‑150 (MQ=255) ctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg > 2:365606/1‑73 (MQ=255) ctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCg < 1:365606/73‑1 (MQ=255) cGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCtt < 2:1772141/150‑1 (MQ=255) gccGCTCCAGCCGAACCTTCGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATATTTGATTGTGTATTCTCTTGAGAGTTCAGGGACTTTTTAAAGTGATGTTGGTGGGGGAAGGATAACTCAGCGCTGCGCGCTTCGCCCTTCggg < 2:75719/148‑1 (MQ=14) caagtcgaaccTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTG < 2:184149‑M1/96‑1 (MQ=255) aCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGG < 1:1149668‑M1/104‑1 (MQ=255) ccTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg < 2:16810/35‑1 (MQ=40) ccTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg > 1:16810/1‑35 (MQ=40) ttgTCGAAGCTTATCATCATTCCCCGCATGGGCTGAATATTTGATTGCGGATTCGTTTGTGATTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGAAACg < 2:1677343/148‑1 (MQ=11) cGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg < 2:1162408/77‑1 (MQ=255) cGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg > 1:1162408/1‑77 (MQ=255) cGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGG < 1:1091198‑M1/96‑1 (MQ=255) cGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGATTGAGAATTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGG > 2:1091198‑M1/1‑96 (MQ=255) ttCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaacctgcggagtggatgccggctgagttactggctggtggcttggggcgctcggg > 2:1227948‑M1/1‑93 (MQ=255) tcATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCTACGGTCTCTCGGTgtc > 1:512553/1‑148 (MQ=255) cATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa > 2:235020/1‑65 (MQ=255) cATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATT < 1:383432‑M1/89‑1 (MQ=255) cATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa < 1:235020/65‑1 (MQ=255) cATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTagtaccatctaagaccatcacggaagatttactgtctgcaccctttggacgctcgggagcc > 2:746288‑M1/1‑89 (MQ=255) ccTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACACAGAGCTCCGAGCTACGCGCGACAGGTCGTTGCATGCGGCGTAGCTCTGTAGCTGGCCCtg > 2:1403206/1‑149 (MQ=14) tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAATCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCTCGCTGTCGCTCGaa > 2:1748561/1‑149 (MQ=255) | TGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAG > NC_000913/1286861‑1287151 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |