Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,292,473 C→G 5.4% intergenic (+108/+36) galU → / ← hns glucose‑1‑phosphate uridylyltransferase/global DNA‑binding transcriptional dual regulator H‑NS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,292,4730CG 5.4% 453.0 / 14.8 148intergenic (+108/+36)galU/hnsglucose‑1‑phosphate uridylyltransferase/global DNA‑binding transcriptional dual regulator H‑NS
Reads supporting (aligned to +/- strand):  ref base C (71/67);  new base G (3/5);  total (76/72)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-01

Only 100 of 154 total aligned reads displayed.

TTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCG  >  NC_000913/1292324‑1292620
                                                                                                                                                     |                                                                                                                                                   
      gCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCgg                                                                                                                                               <  1:1562874/150‑1 (MQ=255)
      cccTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCgg                                                                                                                                               <  2:978838/149‑1 (MQ=255)
         tGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGAt                                                                                                                                            <  1:1415443/150‑1 (MQ=255)
         tGGCTTGAAGAAGAGATGGGCAATAAGAAGTAAGATCGTTATCGTTGTTATCCACGAAACGGCGTTGAGAAATCGATGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGAt                                                                                                                                            <  1:263264/150‑1 (MQ=255)
          ggCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCAGCGGCGGGAtt                                                                                                                                           <  2:392729/150‑1 (MQ=255)
          ggCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCAGCGGCGGGAtt                                                                                                                                           <  2:524769/150‑1 (MQ=255)
            cTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGAtttt                                                                                                                                         >  1:413163/1‑150 (MQ=255)
            cTTGAAGAAGAGATGCGCATTAAGAAGTAACATCCGTATCGGTGTTAACCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAAAAAAAAATCCCGCCGCTGGCGGGAtttt                                                                                                                                         <  2:765774/150‑1 (MQ=255)
              tGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCAGCGGCGGGAtttttt                                                                                                                                       <  1:1321429/150‑3 (MQ=255)
               gaagaaGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCAGCGGCGGGAtttttta                                                                                                                                      <  1:62516/150‑4 (MQ=255)
               gaagaaGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTCAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAg                                                                                                                                      <  1:1822796/150‑1 (MQ=255)
                  gaagaGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCaa                                                                                                                                   >  2:524984/1‑150 (MQ=255)
                   aagaGATGGGCATTAATAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAg                                                                                                                                  <  2:912475/150‑1 (MQ=255)
                     gagaTGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATAGACGCCGATTTTATATAGCTTATTCTTATTTAATTGTCTTAAAGAGGACAATTAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTg                                                                                                                                >  2:871173/1‑150 (MQ=255)
                          gggCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATc                                                                                                                           <  1:1107100/150‑1 (MQ=255)
                             cATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTAc                                                                                                                        <  2:1816373/150‑1 (MQ=255)
                             cATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTAc                                                                                                                        <  2:1823507/150‑1 (MQ=255)
                               ttAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACaa                                                                                                                      <  2:1328472/150‑1 (MQ=255)
                                 aagaagTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACaaaa                                                                                                                    <  1:1249000/150‑1 (MQ=255)
                                  agaagTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAg                                                                                                                   >  2:1778441/1‑150 (MQ=255)
                                    aagTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGAGGGATTTTAAGCAAGTGCAATCTACAAAAgtt                                                                                                                 >  1:223256/1‑148 (MQ=255)
                                     agTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGatt                                                                                                                >  1:1493532/1‑150 (MQ=255)
                                     agTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGatt                                                                                                                >  2:85760/1‑150 (MQ=255)
                                     agTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGATGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGatt                                                                                                                >  2:1192501/1‑150 (MQ=255)
                                      gTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGatta                                                                                                               >  1:1124029/1‑150 (MQ=255)
                                       tAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGattat                                                                                                              >  2:256453/1‑150 (MQ=255)
                                         aCATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTg                                                                                                            >  1:705428/1‑150 (MQ=255)
                                         aCATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTg                                                                                                            <  2:1698481/150‑1 (MQ=255)
                                         aCATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTg                                                                                                            <  1:1809260/150‑1 (MQ=255)
                                          cATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGc                                                                                                           >  1:1309097/1‑150 (MQ=255)
                                          cATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGc                                                                                                           >  2:1644365/1‑150 (MQ=255)
                                           aTCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTAATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCt                                                                                                          <  2:1869047/150‑1 (MQ=255)
                                              cGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCTTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGa                                                                                                       <  2:1646228/150‑1 (MQ=255)
                                              cGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGa                                                                                                       >  1:1593874/1‑150 (MQ=255)
                                                 aTCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCa                                                                                                    <  1:293600/150‑1 (MQ=255)
                                                   cGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAgg                                                                                                  <  1:1215766/150‑1 (MQ=255)
                                                   cGGTGTTATCAACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACNGGACAATAAAAAATCCCNCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAgg                                                                                                  <  1:1862094/150‑1 (MQ=255)
                                                         tATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAtcg                                                                                            <  2:1110583/150‑1 (MQ=255)
                                                         tATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAtcg                                                                                            <  2:899748/150‑1 (MQ=255)
                                                           ataaaaaaaCGGCGTCGATTGCTCAACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAACtcgtc                                                                                          >  1:1167915/7‑150 (MQ=255)
                                                            ccACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTACATTGTCTTAACCCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAtcgtcg                                                                                         <  2:284790/150‑1 (MQ=255)
                                                             cACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGa                                                                                        >  2:1544186/1‑150 (MQ=255)
                                                             cACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGa                                                                                        >  2:1104811/1‑150 (MQ=255)
                                                             cACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGa                                                                                        >  1:962996/1‑150 (MQ=255)
                                                             cACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGa                                                                                        >  1:1378181/1‑150 (MQ=255)
                                                              aCGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAg                                                                                       >  1:486460/1‑150 (MQ=255)
                                                                gAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAggg                                                                                     <  1:1021268/150‑1 (MQ=255)
                                                                gAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAggg                                                                                     >  2:944785/1‑150 (MQ=255)
                                                                  accGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAGAAGATTATTGCTTGATCAGGAAATCGTCGAGGGAt                                                                                   <  1:1110037/148‑1 (MQ=255)
                                                                             gCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTc                                                                        >  2:853118/1‑150 (MQ=255)
                                                                              cAATCGACGCCGTTTTTTTATAGCTTATTCTTATTATATTGTCTTAAACCGTACAATAAAAAAACCCGCCGCTGGCGGGATTTTAAGCAAGTGCAAGCTACAAAAGATTATTGCTTGATCAGGAAATCGTCTAGGGATTTACCTTGCTCa                                                                       <  1:540293/150‑1 (MQ=255)
                                                                              cAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCa                                                                       <  2:341994/150‑1 (MQ=255)
                                                                                    aCGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCAtt                                                                 <  1:1354869/150‑1 (MQ=255)
                                                                                    aCGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCAtt                                                                 <  1:403138/150‑1 (MQ=255)
                                                                                    aCGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCAtt                                                                 <  1:15488/150‑1 (MQ=255)
                                                                                      gCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTgg                                                               >  1:1192484/1‑149 (MQ=255)
                                                                                      gCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGAGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGc                                                               >  1:21405/1‑150 (MQ=255)
                                                                                        cGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTTATCATTAAATCGTCGAGGGATTTACATTGCTCATCCATTGCtt                                                             >  1:1169270/1‑150 (MQ=255)
                                                                                        cGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCtt                                                             >  2:1604088/1‑150 (MQ=255)
                                                                                        cGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCtt                                                             >  2:898479/1‑150 (MQ=255)
                                                                                         gTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCttt                                                            >  2:190665/1‑150 (MQ=255)
                                                                                            tttttATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCtttttt                                                         <  1:1226878/150‑1 (MQ=255)
                                                                                            tttttATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCtttttt                                                         <  1:706001/150‑1 (MQ=255)
                                                                                             ttttATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTg                                                        <  2:320118/150‑1 (MQ=255)
                                                                                                  taGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTAc                                                   >  2:638233/1‑150 (MQ=255)
                                                                                                    gCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAg                                                 >  1:1370410/1‑150 (MQ=255)
                                                                                                       tattcttattAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTg                                              >  2:770369/1‑150 (MQ=255)
                                                                                                            ttattaAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTa                                         >  1:378542/1‑150 (MQ=255)
                                                                                                              attaAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACg                                       >  2:1145351/1‑150 (MQ=255)
                                                                                                               ttaAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACgg                                      <  2:1527015/150‑1 (MQ=255)
                                                                                                                 aaaTTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGcc                                    >  2:1705843/1‑150 (MQ=255)
                                                                                                                  aaTTGTCTTAAACCGGACAATAAAAAATGCAGCCGCTGGCGGGATTTTAAGAAAGTTCTATCTACAAAAGATTATAGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCt                                   <  1:309318/150‑1 (MQ=255)
                                                                                                                    ttGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTg                                 <  1:187033/150‑1 (MQ=255)
                                                                                                                       tCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGAAATCTACAAAAGAGTATTGCTTGACCAGGAAATCGTCGAGGGATTTACCTTGCTCCTCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGcc                              <  2:1272681/150‑1 (MQ=255)
                                                                                                                       gcTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGcc                              <  2:164742/149‑1 (MQ=255)
                                                                                                                        cTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCa                             >  1:1769429/1‑150 (MQ=255)
                                                                                                                          tAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGt                           >  2:71950/1‑150 (MQ=255)
                                                                                                                          tAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGt                           >  1:1330499/1‑150 (MQ=255)
                                                                                                                           aaaCCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTc                          >  2:1311832/1‑150 (MQ=255)
                                                                                                                              ccGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAg                       >  2:1787303/1‑150 (MQ=255)
                                                                                                                                ggACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGt                     >  2:875246/1‑150 (MQ=255)
                                                                                                                                ggACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATCACAGCTGGAGTACGGCCTTGGCCAGTCCAGGt                     <  1:1604088/150‑1 (MQ=255)
                                                                                                                                   cAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGtttt                  <  2:413163/150‑1 (MQ=255)
                                                                                                                                   cAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGtttt                  >  2:1876409/1‑150 (MQ=255)
                                                                                                                                   cAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGtttt                  >  1:494967/1‑150 (MQ=255)
                                                                                                                                    aaTAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGAGCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTCCGGCCTTGGCCAGTCCAGGTTTTa                 <  1:247659/150‑1 (MQ=255)
                                                                                                                                     aTAAAAAATCCCGCCGCGGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCCGTCCAGGTTTTAg                >  1:1638579/1‑150 (MQ=255)
                                                                                                                                       ttaaaaTCCCGCCAGCGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGtt              >  1:590002/3‑150 (MQ=255)
                                                                                                                                       ttaaaaTCCCGCCAGCGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGtt              >  1:968361/3‑150 (MQ=255)
                                                                                                                                       aaaaaaTCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATAAGGAAATCGTCTAGGGATTTACCTTGCTCATCCATTTCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCATGTTTTAttt              >  2:638120/1‑150 (MQ=255)
                                                                                                                                        aaaaaTCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAGAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCCTTGCTTTTTTGCTTACAGCTGGAGTACGGCCTTGTCCTGTCCAGGTTTTAGttt             >  1:1123996/1‑150 (MQ=255)
                                                                                                                                          aaaTCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCg           <  1:1761555/150‑1 (MQ=255)
                                                                                                                                          aaaTCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCg           <  2:1398339/150‑1 (MQ=255)
                                                                                                                                            aTCCCGGCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGAGCAGGAAATCGGCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGcc         <  2:127285/150‑1 (MQ=255)
                                                                                                                                             tCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCg        <  1:619141/150‑1 (MQ=255)
                                                                                                                                             tCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCg        <  1:85760/150‑1 (MQ=255)
                                                                                                                                                cgccgcTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGttt     <  2:1364697/150‑1 (MQ=255)
                                                                                                                                                 gccgtTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGTCAGTCCAGGTTTTAGTTTCGCCGtttt    <  1:1192501/150‑1 (MQ=255)
                                                                                                                                                   cgcTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCg  <  2:1309097/150‑1 (MQ=255)
                                                                                                                                                   cgcTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAGCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCg  <  1:1001595/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTTAAAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCG  >  NC_000913/1292324‑1292620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: