Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,292,474 T→C 5.2% intergenic (+109/+35) galU → / ← hns glucose‑1‑phosphate uridylyltransferase/global DNA‑binding transcriptional dual regulator H‑NS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,292,4740TC 5.2% 466.3 / 11.1 153intergenic (+109/+35)galU/hnsglucose‑1‑phosphate uridylyltransferase/global DNA‑binding transcriptional dual regulator H‑NS
Reads supporting (aligned to +/- strand):  ref base T (72/72);  new base C (3/5);  total (76/77)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.01e-02

Only 100 of 153 total aligned reads displayed.

AAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCG  >  NC_000913/1292328‑1292620
                                                                                                                                                  |                                                                                                                                                  
aaGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGc                                                                                                                                                 >  1:617404/1‑150 (MQ=255)
  gCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCgg                                                                                                                                               <  1:1562874/150‑1 (MQ=255)
  cccTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCgg                                                                                                                                               <  2:978838/149‑1 (MQ=255)
     tGGCTTGAAGAAGAGATGGGCAATAAGAAGTAAGATCGTTATCGTTGTTATCCACGAAACGGCGTTGAGAAATCGATGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGAt                                                                                                                                            <  1:263264/150‑1 (MQ=255)
      ggCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCAGCGGCGGGAtt                                                                                                                                           <  2:524769/150‑1 (MQ=255)
      ggCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCAGCGGCGGGAtt                                                                                                                                           <  2:392729/150‑1 (MQ=255)
       gCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGAttt                                                                                                                                          >  1:1837353/1‑150 (MQ=255)
        cTTGAAGAAGAGATGCGCATTAAGAAGTAACATCCGTATCGGTGTTAACCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAAAAAAAAATCCCGCCGCTGGCGGGAtttt                                                                                                                                         <  2:765774/150‑1 (MQ=255)
            aagaagAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGc                                                                                                                                     >  2:714430/1‑150 (MQ=255)
               aagaGATGGGCATTAATAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAg                                                                                                                                  <  2:912475/150‑1 (MQ=255)
                 gagaTGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATAGACGCCGATTTTATATAGCTTATTCTTATTTAATTGTCTTAAAGAGGACAATTAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTg                                                                                                                                >  2:871173/1‑150 (MQ=255)
                  agaTGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGc                                                                                                                               <  1:379732/150‑1 (MQ=255)
                   gaTGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCa                                                                                                                              >  2:1286015/1‑150 (MQ=255)
                    aTGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCaa                                                                                                                             >  1:1398339/1‑150 (MQ=255)
                     tGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAAt                                                                                                                            >  2:1001595/1‑150 (MQ=255)
                      gggCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATc                                                                                                                           <  1:1107100/150‑1 (MQ=255)
                         cATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTAc                                                                                                                        >  2:1373677/1‑150 (MQ=255)
                         cATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTAc                                                                                                                        <  2:1816373/150‑1 (MQ=255)
                           ttAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACaa                                                                                                                      <  2:1328472/150‑1 (MQ=255)
                             aataagTAACATCCGTATCGGTGTTATCCACGAAAAGGCGTTGATCATTCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACaaaa                                                                                                                    <  1:326594/147‑1 (MQ=255)
                              agaagTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAg                                                                                                                   >  2:1778441/1‑150 (MQ=255)
                               gaagTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGa                                                                                                                  >  1:507345/1‑150 (MQ=255)
                                 agTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGatt                                                                                                                >  1:1493532/1‑150 (MQ=255)
                                 agTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGatt                                                                                                                >  2:85760/1‑150 (MQ=255)
                                  gTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGatta                                                                                                               >  1:1124029/1‑150 (MQ=255)
                                   tAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGattat                                                                                                              >  2:256453/1‑150 (MQ=255)
                                    aaCATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTATTATAGCTTATTATTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGATGACGGGATTATAAGCAAGTGCAATCAACAAAAGattatt                                                                                                             >  1:164742/1‑150 (MQ=255)
                                     aCATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTg                                                                                                            <  1:1809260/150‑1 (MQ=255)
                                     aCATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTg                                                                                                            >  1:705428/1‑150 (MQ=255)
                                     aCATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTg                                                                                                            <  2:1698481/150‑1 (MQ=255)
                                      cATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGc                                                                                                           >  2:1644365/1‑150 (MQ=255)
                                      cATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGc                                                                                                           >  1:1309097/1‑150 (MQ=255)
                                       aTCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTAATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCt                                                                                                          <  2:1869047/150‑1 (MQ=255)
                                          cGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCTTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGa                                                                                                       <  2:1646228/150‑1 (MQ=255)
                                               cGGTGTTATCAACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACNGGACAATAAAAAATCCCNCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAgg                                                                                                  <  1:1862094/150‑1 (MQ=255)
                                                  tgtTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGaaa                                                                                               <  2:1837353/150‑1 (MQ=255)
                                                  tgtTATCCACGAAACGGCGTTGAGCAATCGAAGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGaaa                                                                                               <  1:1184011/150‑1 (MQ=255)
                                                     tATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAtcg                                                                                            <  2:899748/150‑1 (MQ=255)
                                                     tATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAtcg                                                                                            <  2:1321565/150‑1 (MQ=255)
                                                       tacACTAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGGTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAtcgtc                                                                                          <  2:58952/148‑1 (MQ=255)
                                                        ccACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTACATTGTCTTAACCCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAtcgtcg                                                                                         <  2:284790/150‑1 (MQ=255)
                                                         cACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGa                                                                                        >  2:1104811/1‑150 (MQ=255)
                                                         cACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGa                                                                                        >  2:1544186/1‑150 (MQ=255)
                                                         cACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGa                                                                                        >  1:962996/1‑150 (MQ=255)
                                                         cACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGa                                                                                        >  1:1239697/1‑150 (MQ=255)
                                                         cACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGa                                                                                        >  1:1378181/1‑150 (MQ=255)
                                                          aaaaaaCGGCGTCGATTGCTCAACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAg                                                                                       >  1:1364697/4‑150 (MQ=255)
                                                          aCGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAg                                                                                       >  1:486460/1‑150 (MQ=255)
                                                           cGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAgg                                                                                      <  1:1573259/150‑1 (MQ=255)
                                                            gAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAggg                                                                                     <  1:1021268/150‑1 (MQ=255)
                                                            gAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAggg                                                                                     >  2:944785/1‑150 (MQ=255)
                                                              accGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAGAAGATTATTGCTTGATCAGGAAATCGTCGAGGGAt                                                                                   <  1:1110037/148‑1 (MQ=255)
                                                              aaCGGCGTTGATCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGAt                                                                                   <  1:1504834/150‑1 (MQ=255)
                                                                        aGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCt                                                                         <  1:1286015/150‑1 (MQ=255)
                                                                         gCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTc                                                                        >  2:853118/1‑150 (MQ=255)
                                                                         gCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTc                                                                        >  1:2777/1‑150 (MQ=255)
                                                                          cAATCGACGCCGTTTTTTTATAGCTTATTCTTATTATATTGTCTTAAACCGTACAATAAAAAAACCCGCCGCTGGCGGGATTTTAAGCAAGTGCAAGCTACAAAAGATTATTGCTTGATCAGGAAATCGTCTAGGGATTTACCTTGCTCa                                                                       <  1:540293/150‑1 (MQ=255)
                                                                          cAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCa                                                                       <  2:341994/150‑1 (MQ=255)
                                                                                aCGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCAtt                                                                 <  1:403138/150‑1 (MQ=255)
                                                                                  gCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGAGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGc                                                               >  1:21405/1‑150 (MQ=255)
                                                                                    cGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTTATCATTAAATCGTCGAGGGATTTACATTGCTCATCCATTGCtt                                                             >  1:1169270/1‑150 (MQ=255)
                                                                                    cGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCtt                                                             >  2:1604088/1‑150 (MQ=255)
                                                                                    cGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCtt                                                             >  2:898479/1‑150 (MQ=255)
                                                                                     gTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCttt                                                            >  2:190665/1‑150 (MQ=255)
                                                                                        tttttATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCtttttt                                                         <  1:1226878/150‑1 (MQ=255)
                                                                                         ttttATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTg                                                        <  2:320118/150‑1 (MQ=255)
                                                                                            tataGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGAtt                                                     <  2:174332/150‑1 (MQ=255)
                                                                                                gCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCTTCGAGGGATTTACATTGCTCATCCATTGCTTTTTTGATTACAg                                                 >  1:1214091/1‑150 (MQ=255)
                                                                                                   tattcttattAAATTGTCTTAAACCGGACAATAAAAATTCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGTTTATTGCTTGTTCAGGAAATCGTCGAGGGATTTACCTTGCTCCTCCTTTGCTTTTTTGATTACAGCTg                                              <  1:1322849/150‑1 (MQ=255)
                                                                                                   tattcttattAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGTGCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTg                                              <  1:163857/150‑1 (MQ=255)
                                                                                                   tattcttattAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTg                                              >  1:240061/1‑150 (MQ=255)
                                                                                                   tattcttattAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTg                                              >  2:770369/1‑150 (MQ=255)
                                                                                                      tcttattAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAg                                           <  2:1200532/150‑1 (MQ=255)
                                                                                                          attaAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACg                                       >  2:1145351/1‑150 (MQ=255)
                                                                                                           ttaAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACgg                                      <  2:1527015/150‑1 (MQ=255)
                                                                                                              aaTTGTCTTAAACCGGACAATAAAAAATGCAGCCGCTGGCGGGATTTTAAGAAAGTTCTATCTACAAAAGATTATAGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCt                                   <  1:309318/150‑1 (MQ=255)
                                                                                                              aaTTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCTATCTACAAAAGATTATTGCTTGTTCAGGAAATCGTCTAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCt                                   <  1:45363/150‑1 (MQ=255)
                                                                                                                  gTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGc                               <  1:1874008/150‑1 (MQ=255)
                                                                                                                   tCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGcc                              <  1:714430/150‑1 (MQ=255)
                                                                                                                   tCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGAAATCTACAAAAGAGTATTGCTTGACCAGGAAATCGTCGAGGGATTTACCTTGCTCCTCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGcc                              <  2:1272681/150‑1 (MQ=255)
                                                                                                                     ttAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAg                            >  2:1666510/1‑150 (MQ=255)
                                                                                                                      tAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGt                           >  2:71950/1‑150 (MQ=255)
                                                                                                                      tAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGt                           >  1:672487/1‑150 (MQ=255)
                                                                                                                      tAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGt                           >  1:1330499/1‑150 (MQ=255)
                                                                                                                            ggACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGt                     >  2:875246/1‑150 (MQ=255)
                                                                                                                            ggACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATCACAGCTGGAGTACGGCCTTGGCCAGTCCAGGt                     <  1:1604088/150‑1 (MQ=255)
                                                                                                                               cAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGtttt                  <  2:413163/150‑1 (MQ=255)
                                                                                                                               cAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGtttt                  <  2:1122515/150‑1 (MQ=255)
                                                                                                                               cAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGtttt                  >  2:1876409/1‑150 (MQ=255)
                                                                                                                                   aaaaaaTCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATAAGGAAATCGTCTAGGGATTTACCTTGCTCATCCATTTCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCATGTTTTAttt              >  2:638120/1‑150 (MQ=255)
                                                                                                                                    aaaaaTCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAGAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCCTTGCTTTTTTGCTTACAGCTGGAGTACGGCCTTGTCCTGTCCAGGTTTTAGttt             >  1:1123996/1‑150 (MQ=255)
                                                                                                                                      aaaTCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCg           <  2:1398339/150‑1 (MQ=255)
                                                                                                                                      aaaTCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCg           <  1:1761555/150‑1 (MQ=255)
                                                                                                                                       aaTCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGc          >  2:1806641/1‑150 (MQ=255)
                                                                                                                                         tCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCg        <  1:85760/150‑1 (MQ=255)
                                                                                                                                         tCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCg        <  1:619141/150‑1 (MQ=255)
                                                                                                                                             gccgtTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGTCAGTCCAGGTTTTAGTTTCGCCGtttt    <  1:1192501/150‑1 (MQ=255)
                                                                                                                                               cgcTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCg  <  1:1778441/150‑1 (MQ=255)
                                                                                                                                               cgcTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCg  <  2:1309097/150‑1 (MQ=255)
                                                                                                                                               cgcTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAAGCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCg  <  1:1001595/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
AAGCCTGGCTTGAAGAAGAGATGGGCATTAAGAAGTAACATCCGTATCGGTGTTATCCACGAAACGGCGTTGAGCAATCGACGCCGTTTTTTTATAGCTTATTCTTATTAAATTGTCTTAAACCGGACAATAAAAAATCCCGCCGCTGGCGGGATTTTAAGCAAGTGCAATCTACAAAAGATTATTGCTTGATCAGGAAATCGTCGAGGGATTTACCTTGCTCATCCATTGCTTTTTTGATTACAGCTGGAGTACGGCCTTGGCCAGTCCAGGTTTTAGTTTCGCCGTTTTCG  >  NC_000913/1292328‑1292620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑