Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,311,830 A→G 7.9% intergenic (+22/+18) tonB → / ← yciA membrane spanning protein in TonB‑ExbB‑ExbD transport complex/acyl‑CoA esterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,311,8300AG 7.9% 305.7 / 14.0 115intergenic (+22/+18)tonB/yciAmembrane spanning protein in TonB‑ExbB‑ExbD transport complex/acyl‑CoA esterase
Reads supporting (aligned to +/- strand):  ref base A (52/52);  new base G (5/4);  total (59/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.55e-01

Only 100 of 116 total aligned reads displayed.

TGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAAT  >  NC_000913/1311682‑1311979
                                                                                                                                                    |                                                                                                                                                     
tGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGAc                                                                                                                                                      >  1:251442/1‑150 (MQ=255)
tGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGAc                                                                                                                                                      >  1:613213/1‑150 (MQ=255)
  cGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGTTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCg                                                                                                                                                    <  1:1275567/150‑1 (MQ=255)
  cGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCg                                                                                                                                                    >  1:732074/1‑150 (MQ=255)
     aCATGATGGAGCGTGAGCCGAAAACTGCGAAGCGCACATGGCGTTAGGAGCCGGGTAAGCCAGGCCGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAACGTCAAAAGCCTCCGACCGGAg                                                                                                                                                 <  2:366460/150‑1 (MQ=255)
      cATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAgg                                                                                                                                                >  1:1838998/1‑150 (MQ=255)
       aTGTTTGAGCGTGAGTTGAAAAATGCGATGCGCAGATGTCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGc                                                                                                                                               <  1:1132000/150‑1 (MQ=255)
          tttGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCttt                                                                                                                                            >  2:171337/1‑150 (MQ=255)
           ttGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCtttt                                                                                                                                           >  2:1638448/1‑150 (MQ=255)
           ttGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCtttt                                                                                                                                           >  1:1493167/1‑150 (MQ=255)
            tGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGACTCCGGTCGGAGGCTTTTg                                                                                                                                          <  2:881990/150‑1 (MQ=255)
             gAGCGTGAGGTGAAAACTGCGATGCGCAGATGGCCTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGa                                                                                                                                         <  1:762926/150‑1 (MQ=255)
             gAGCGTGAGGGGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGGAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGa                                                                                                                                         <  1:210040/150‑1 (MQ=255)
              cgCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGAc                                                                                                                                        <  1:560199/149‑1 (MQ=255)
              cgCGTGAGGTGAAAAATGCGATGCGCAGATGGCGGTATGAGCCGGGTAAGCCAGGCAGTGGGATGGTGGGGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGGTCGGAGGCTTTTGAc                                                                                                                                        <  1:1126529/149‑1 (MQ=255)
              aGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGAc                                                                                                                                        >  1:988350/1‑150 (MQ=255)
              aGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGAc                                                                                                                                        >  1:110248/1‑150 (MQ=255)
               gCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACt                                                                                                                                       <  2:1509776/150‑1 (MQ=255)
                cGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTa                                                                                                                                      >  2:779175/1‑150 (MQ=255)
                    aGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGATTATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGCGCGGAGGCTTTTGACTATTAc                                                                                                                                  >  2:1128499/1‑150 (MQ=255)
                     ggTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACt                                                                                                                                 >  1:1714806/1‑150 (MQ=255)
                     ggTGAAAAATGCGAGGCGCAGATGGCGTTATGAGAAGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCCCCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCAGACCGGAGGCTTTTGACTATTACt                                                                                                                                 >  2:938545/1‑150 (MQ=255)
                      gTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCGTTTGACTATTACTc                                                                                                                                >  1:1421508/1‑150 (MQ=255)
                       ggAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCa                                                                                                                               <  1:1839257/149‑1 (MQ=255)
                        gAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCaa                                                                                                                              >  2:1211203/1‑150 (MQ=255)
                         aaaaaTGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTATAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAAc                                                                                                                             <  1:774250/150‑1 (MQ=255)
                          aaaaTGCGATGCGGAGTTGTCGTTATGAGGCGGTAAAGGCAGGCAGTGGGATTGTGGTGAATATGCTTTTTAAAATTACCGGCACCAGCGATTTTCAGTAAGCACAAAGTTTAAATCCTCCTACCGGAGGCTTTTGACTATTACTCAACa                                                                                                                            <  2:699417/150‑1 (MQ=255)
                              tgcgatgcgCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTa                                                                                                                        >  2:1203983/1‑150 (MQ=255)
                                cgatgcgCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAg                                                                                                                      >  1:1231619/1‑150 (MQ=255)
                                     cgcAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGcgcg                                                                                                                 <  2:569208/150‑1 (MQ=255)
                                          aTGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGtt                                                                                                            <  1:505226/150‑1 (MQ=255)
                                            ggCGTTATGAGCCGGGTAAGACAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACTGCACCACCGAAATTCAGTAAGCTGAAAGTCTAAAGGCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGtttt                                                                                                          <  1:77678/150‑1 (MQ=255)
                                              ggTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTcc                                                                                                        <  2:1547128/149‑1 (MQ=255)
                                              cGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTcc                                                                                                        <  1:1725805/150‑1 (MQ=255)
                                              cGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTcc                                                                                                        <  1:1711616/150‑1 (MQ=255)
                                               gTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCt                                                                                                       <  2:201425/150‑1 (MQ=255)
                                                 tATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTc                                                                                                     <  2:1053120/150‑1 (MQ=255)
                                                    gAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAgg                                                                                                  <  2:1231619/150‑1 (MQ=255)
                                                    gAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAgg                                                                                                  >  1:1135649/1‑150 (MQ=255)
                                                    gAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAgg                                                                                                  <  2:1580256/150‑1 (MQ=255)
                                                      gCCGGGTAAGCCAGGCAGTGGGATTGTGGTTAATATCCTGTTTATAATTAACGGCACCACCGAAATTCAGTAAGCATAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGAt                                                                                                <  2:1408701/150‑1 (MQ=255)
                                                      gCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGAt                                                                                                <  1:1511208/150‑1 (MQ=255)
                                                          ggTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAAc                                                                                            >  1:869392/1‑150 (MQ=255)
                                                            tAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACcg                                                                                          >  2:560/1‑150 (MQ=255)
                                                               gCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGa                                                                                       >  2:1694472/1‑150 (MQ=255)
                                                               gCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGa                                                                                       >  2:1625994/1‑150 (MQ=255)
                                                                 cAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACa                                                                                     >  2:701420/1‑150 (MQ=255)
                                                                 cAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACa                                                                                     >  2:619206/1‑150 (MQ=255)
                                                                           taTTGTGGGGAATATCCTGTATAAATTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGGCAAAAGCCTCCGACCGGAGGCTTTTGACTATTAATCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAaata                                                                           <  2:327783/149‑1 (MQ=255)
                                                                           gATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAaata                                                                           <  1:176631/150‑1 (MQ=255)
                                                                            aTTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAaataa                                                                          >  2:787869/1‑150 (MQ=255)
                                                                            aTTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAaataa                                                                          >  2:1520699/1‑150 (MQ=255)
                                                                               gtggtgAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGc                                                                       <  2:1274619/150‑1 (MQ=255)
                                                                               gtggtgAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGc                                                                       <  1:1028134/150‑1 (MQ=255)
                                                                                  gtgAAAATCCTGTTTAAAATATACGGCACCACCGAAATTCAGTAAGCAGAAAGTCTAAAGCCTTCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTc                                                                    <  1:1148529/150‑1 (MQ=255)
                                                                                          ccTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCtt                                                            >  1:453962/1‑150 (MQ=255)
                                                                                             gTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTtat                                                         >  2:819989/1‑150 (MQ=255)
                                                                                              tttAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTtata                                                        <  2:1685060/150‑1 (MQ=255)
                                                                                                tAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTTAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGc                                                      >  1:1692687/1‑150 (MQ=255)
                                                                                                 aaaaTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACATGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCg                                                     >  2:1846547/1‑150 (MQ=255)
                                                                                                  aaaTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGt                                                    >  2:635483/1‑150 (MQ=255)
                                                                                                  aaaTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGt                                                    <  2:1197391/150‑1 (MQ=255)
                                                                                                   aaTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGtt                                                   <  2:251442/150‑1 (MQ=255)
                                                                                                    aTTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTACTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTg                                                  >  1:738635/1‑150 (MQ=255)
                                                                                                     ttAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGc                                                 <  2:487877/150‑1 (MQ=255)
                                                                                                      tAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGcc                                                <  1:695118/150‑1 (MQ=255)
                                                                                                       aaCGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTAGCTTTATAGCGTTGccc                                               >  1:1343517/1‑150 (MQ=255)
                                                                                                          ggCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAAt                                            <  1:332719/150‑1 (MQ=255)
                                                                                                            caccacCGAAATTCAGTAAGCATAAAGTCAAAATCCTCCTAACTGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTg                                          <  2:895341/150‑1 (MQ=255)
                                                                                                            caccacCGAAAATCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTAGCCCAATTg                                          >  2:1002726/1‑150 (MQ=255)
                                                                                                               caccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGtt                                       <  1:1565489/150‑1 (MQ=255)
                                                                                                                 ccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACAAACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCa                                     >  2:111250/1‑150 (MQ=255)
                                                                                                                 ccGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCCACCGGAGGCTTTTGACTATTACTCAACAGGGAAGGCGCGACGTTTTCCTTCAGGATCAACCGCGACATACATAAATAAAGCTTCTGTCGCTTTATAGCGTTGCCCAAATGGttaa                                     >  1:866137/1‑148 (MQ=255)
                                                                                                                     aaTTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACg                                 >  2:685956/1‑150 (MQ=255)
                                                                                                                       ttCAGTAAGCAGATAGTCTAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATTATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCt                               <  1:514610/150‑1 (MQ=255)
                                                                                                                       ttCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCt                               <  1:779175/150‑1 (MQ=255)
                                                                                                                          aGTAAGCAGAAAGTCAAAAGCCTCCGCCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGACGTTTTCCTTCAGGATCAACCGCCACATACTTAAATAAAGCTTCTTTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACt                            >  2:423466/1‑150 (MQ=255)
                                                                                                                            tAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACttt                          <  1:1780031/150‑1 (MQ=255)
                                                                                                                              aGCAGAAAGTCAAAAGCCTCCGACCTGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACttttt                        <  1:300429/150‑1 (MQ=255)
                                                                                                                              aGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGGTTTCCTTCAGGATCAACCGCGCCATACTTAAATAATGCTTCTGTCGCTTTATACCGTTGCCCATTTGGTTCAGACGCTACttttt                        >  2:177687/1‑150 (MQ=255)
                                                                                                                                cAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTc                      <  1:1047691/150‑1 (MQ=255)
                                                                                                                                cAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTc                      >  1:482912/1‑150 (MQ=255)
                                                                                                                                cAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACATAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACtttttta                      >  1:161031/1‑149 (MQ=255)
                                                                                                                                    aaGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCAccc                  <  2:1752159/150‑1 (MQ=255)
                                                                                                                                    aaGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCAccc                  >  1:216720/1‑150 (MQ=255)
                                                                                                                                      gTCAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCcac                >  1:275932/1‑150 (MQ=255)
                                                                                                                                      gTCAAAAGCCTCCGACCGTAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCcac                <  1:1592823/150‑1 (MQ=255)
                                                                                                                                       tCAAAAGCCTCCGGTCGGAGGCTTTTGACTGTTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCGcccgcc               >  1:1255034/1‑147 (MQ=255)
                                                                                                                                        cAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCCCCCacac              >  2:115028/1‑150 (MQ=255)
                                                                                                                                        cAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCacac              >  1:9661/1‑150 (MQ=255)
                                                                                                                                        cAAAAGCCTCCGGTCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCacac              >  1:1573698/1‑150 (MQ=255)
                                                                                                                                        cAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCacac              <  2:1493167/150‑1 (MQ=255)
                                                                                                                                        cAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCacac              >  2:207518/1‑150 (MQ=255)
                                                                                                                                         aaaaGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACt             >  2:488512/1‑150 (MQ=255)
                                                                                                                                          aaaGCCTCCGACCGGAGGCATTTGACTATTACTCAACAGGTAAGGCGCTAGGTTTTCCTTCAGGATCAACCGCGCCATACTTAAATAATGCATCTGTCGCTTTATTGCGTTGCCCAATTGGTTAAGACGCTAGTTTTTTCACCAacacat            >  2:111808/1‑148 (MQ=255)
                                                                                                                                              ccTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAat        <  1:619206/150‑1 (MQ=255)
                                                                                                                                               gttcGACCGGAGGCTTTTGACTATTACTCAACAGGTATGGCGCCAGGTTTTCCTTCAGGATCAAGCGCGACATACTTTAATAATGCTTCTGTGGCTTTATAGCGTTGCCCAAATGGTTCAGACGCTAATTTTTTCACCCACACTTCAata       <  1:1456641/147‑1 (MQ=255)
                                                                                                                                                 ccGACCGCAGGCTTTAGACTATTACTCAACAGGTAAGGCCCGAGGTTTTCCTTCAGGATCAACCGAGACAAACTAAAATAAAGCTTCTCTCGCTTTATAGCGTTGCCCAATTGGTTCAGCCGCAACTTTTTTCACCCACACATCAatata     >  2:1684347/1‑149 (MQ=255)
                                                                                                                                                   gACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTaa   <  2:74360/150‑1 (MQ=255)
                                                                                                                                                    aCCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAAt  <  1:803081/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGCGAACATGTTTGAGCGTGAGGTGAAAAATGCGATGCGCAGATGGCGTTATGAGCCGGGTAAGCCAGGCAGTGGGATTGTGGTGAATATCCTGTTTAAAATTAACGGCACCACCGAAATTCAGTAAGCAGAAAGTCAAAAGCCTCCGACCGGAGGCTTTTGACTATTACTCAACAGGTAAGGCGCGAGGTTTTCCTTCAGGATCAACCGCGACATACTTAAATAATGCTTCTGTCGCTTTATAGCGTTGCCCAATTGGTTCAGACGCTACTTTTTTCACCCACACTTCAATATTAAT  >  NC_000913/1311682‑1311979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: