Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,322,398 C→G 5.9% V183V (GTG→GTC)  trpE ← component I of anthranilate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,322,3980CG 5.9% 369.4 / 12.8 119V183V (GTG→GTC) trpEcomponent I of anthranilate synthase
Reads supporting (aligned to +/- strand):  ref base C (50/62);  new base G (5/2);  total (55/64)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.71e-01

Only 100 of 121 total aligned reads displayed.

TGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTC  >  NC_000913/1322249‑1322545
                                                                                                                                                     |                                                                                                                                                   
tGCGTCACGGCACCCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGATCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATc                                                                                                                                                     <  2:1156479/150‑1 (MQ=255)
  cGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCAc                                                                                                                                                   <  1:1748190/150‑1 (MQ=255)
    gCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCa                                                                                                                                                 >  2:1249645/1‑150 (MQ=255)
    gCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCa                                                                                                                                                 <  1:174272/150‑1 (MQ=255)
    gCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCa                                                                                                                                                 <  1:1487418/150‑1 (MQ=255)
     cACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGTGTGCTTTTTTTCTGATGGTCAATCACCAt                                                                                                                                                <  1:1173260/150‑1 (MQ=255)
        ggAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAg                                                                                                                                             >  2:1036886/1‑150 (MQ=255)
         gAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTGATTGACCATCAga                                                                                                                                            <  2:1084500/150‑2 (MQ=255)
          aaaCCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTGATTGACCATCAgaa                                                                                                                                           <  1:1097223/150‑3 (MQ=255)
              cACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCAACATCAGCGTTTc                                                                                                                                       >  1:1380534/1‑150 (MQ=255)
                  ggCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCAATCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCg                                                                                                                                   <  2:1355432/150‑1 (MQ=255)
                  ggCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCACAGAGACGTTGTTTTTCTTCTTCATTCGGAGAAAACAGGCTGGCCTGAAAACGGGAGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCg                                                                                                                                   >  1:1873370/1‑150 (MQ=255)
                  ggCAGCGGCGGCGCGGCTTCGGTCAGTTGCTCAAGTAGTTCGTTCAGGCGAGCAGTGAGACGTAGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCATTCACCATCCGCGTTTCAGCg                                                                                                                                   >  1:316200/1‑150 (MQ=255)
                    cAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTATCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCgag                                                                                                                                 <  2:1065437/150‑1 (MQ=255)
                     aGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCgaga                                                                                                                                <  2:415867/150‑1 (MQ=255)
                      gcggcggcgCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGAt                                                                                                                               >  1:1556573/1‑150 (MQ=255)
                      gcggcggcgCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGAt                                                                                                                               >  1:636590/1‑150 (MQ=255)
                       cggcggcgCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCCGCGAGATa                                                                                                                              >  2:1251518/1‑150 (MQ=255)
                           ggcgcggcTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATaaaaa                                                                                                                          >  2:851099/1‑150 (MQ=255)
                              gcggcTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAg                                                                                                                       <  2:53726/150‑1 (MQ=255)
                               cggcTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGAGTTTCAGCGAGATAAAAACAGa                                                                                                                      <  1:1164136/150‑1 (MQ=255)
                                   ttCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATc                                                                                                                  <  2:849404/150‑1 (MQ=255)
                                      ggTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAgg                                                                                                               >  1:208707/1‑150 (MQ=255)
                                      ggTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAgg                                                                                                               <  2:527224/150‑1 (MQ=255)
                                      ggTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAgg                                                                                                               <  2:741068/150‑1 (MQ=255)
                                       gTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAggg                                                                                                              >  1:1475360/1‑150 (MQ=255)
                                        gcAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGc                                                                                                             <  1:229156/149‑1 (MQ=255)
                                         cAGTTGCTGAAGTAGTTCGTTCAGGCGAGCAGAGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGACTGAATACGGGTGCTTTTTTTCTGATGATCAAACACCATCAGCGTTTCAGAGAGATAAAAACAGAAATCAGGGCa                                                                                                            >  2:564585/1‑150 (MQ=255)
                                          aGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGGGATAAAAACAGAAATCAGGGCAg                                                                                                           >  2:199319/1‑150 (MQ=255)
                                          aGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAg                                                                                                           >  2:1532353/1‑150 (MQ=255)
                                            gtGCTGACGTAGTTCGGTCAGGCGAGCAGTGAGACGTTGTTTTTCATCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGtt                                                                                                         <  2:939818/149‑1 (MQ=255)
                                                 taCGTAGTTCGTTCAGGCGAGGAGTGAGAAGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGATTACGGGTGCTTTTTTTCTTATGGTCAATCAGCATCAGCGTTGCAGCGATATAAAAACAGATATCAGGGCAGTTAtttt                                                                                                    <  2:612985/149‑1 (MQ=255)
                                                 gACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGTGATAAAAACAGAAATCAGGGCAGTTAtttt                                                                                                    >  2:1570349/1‑150 (MQ=255)
                                                 gACGTAGGTCGGTCAGGCGAGCAGGGAGACGGTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGCCCTGAATACGGGTGCTTTTTTTCTGATGGCCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTAtttt                                                                                                    <  2:1182620/150‑1 (MQ=255)
                                                    gTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCg                                                                                                 <  2:1532577/150‑1 (MQ=255)
                                                     tAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGc                                                                                                <  2:1130344/150‑1 (MQ=255)
                                                      tgttcgttcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCt                                                                                               <  1:1614662/149‑1 (MQ=255)
                                                       gttcgttcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCATGGCAGTTATTTTCCGCTg                                                                                              <  1:1749879/150‑1 (MQ=255)
                                                          cgttcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACa                                                                                           >  2:1270610/1‑150 (MQ=255)
                                                           gttcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAg                                                                                          >  1:924524/1‑150 (MQ=255)
                                                           gttcAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAg                                                                                          >  2:1064589/1‑150 (MQ=255)
                                                                ggCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGCCAGTTGCg                                                                                     >  2:951299/1‑150 (MQ=255)
                                                                ggCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCg                                                                                     <  1:1814794/150‑1 (MQ=255)
                                                                ggCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCg                                                                                     >  2:1119674/1‑150 (MQ=255)
                                                                  cGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGGCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGt                                                                                   <  1:1757102/150‑1 (MQ=255)
                                                                     gCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTaaa                                                                                <  1:545912/150‑1 (MQ=255)
                                                                        gTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCt                                                                             <  1:1819903/150‑1 (MQ=255)
                                                                          gagaCGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTc                                                                           >  1:845404/1‑150 (MQ=255)
                                                                          gagaCGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTc                                                                           <  1:1197960/150‑1 (MQ=255)
                                                                          gagaCGGTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACACAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTc                                                                           <  1:930450/150‑1 (MQ=255)
                                                                           agaCGTTGTTTTTCTTCTTCATTCGGAGCTCACCGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGGCAATCACCATCAGCGTTTCAGCGAGATAAAACCGGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCa                                                                          <  2:688194/150‑1 (MQ=255)
                                                                           agaCGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCa                                                                          <  2:957964/150‑1 (MQ=255)
                                                                            gaCGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCaa                                                                         <  2:1465298/150‑1 (MQ=255)
                                                                               gttgttTTTCTTCTTCAATCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATc                                                                      <  1:69684/150‑1 (MQ=255)
                                                                                ttgttTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGCCAGTTGCGGTAAATCTTCAAATcc                                                                     >  2:1166813/1‑150 (MQ=255)
                                                                                 ggttTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATccc                                                                    <  2:665050/149‑1 (MQ=255)
                                                                                     tttCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCca                                                                <  1:979180/150‑1 (MQ=255)
                                                                                       tcttcttaATTCGGTGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATAAGAGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCcaca                                                              <  2:166664/150‑1 (MQ=255)
                                                                                       tattattcATTAGGATCAAACAGGCTGTCCTGAATACGGGGTCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCATCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCcaca                                                              <  2:161082/145‑1 (MQ=255)
                                                                                        cttcttcATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCCCCATCAGCGTTTCAGCGAGATAAAAACAGAAATAAGGGCAGTTAGTTTCCGCTGACAGTTGCGGGAAATCTTCAAATCCCGCcacca                                                             >  2:279209/1‑148 (MQ=255)
                                                                                         ttcttcATTCGGAGCAAACAGGCTGGCCTTAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGCGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAg                                                            <  2:1689139/150‑1 (MQ=255)
                                                                                           cttcATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGt                                                          >  1:1573625/1‑150 (MQ=255)
                                                                                           cttcATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGt                                                          >  1:919392/1‑150 (MQ=255)
                                                                                             tcATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCa                                                        <  2:1808629/150‑1 (MQ=255)
                                                                                              cATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGTGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCAt                                                       >  2:1380539/1‑150 (MQ=255)
                                                                                                 tCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAag                                                    <  2:417228/150‑1 (MQ=255)
                                                                                                   ggAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAagag                                                  >  1:505178/1‑150 (MQ=255)
                                                                                                    gAGCAAACAGGCTGGCCTGAATACGGGTGTTTTTTTTCTGATGGTCAATCACCATCAGAGTTTCAGCTAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGATGTAAATCTTCAAATCCCGCCACAAGGTCATAAgaga                                                 <  1:897972/150‑1 (MQ=255)
                                                                                                    gAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCAACAAGGTCATAAgaga                                                 >  2:836368/1‑150 (MQ=255)
                                                                                                     aGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGaa                                                >  1:1076945/1‑150 (MQ=255)
                                                                                                          aCAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGgc                                           <  2:1485007/150‑1 (MQ=255)
                                                                                                          aCAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGgc                                           >  1:427244/1‑150 (MQ=255)
                                                                                                          aCAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTGCAGCGAGATAAACACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGgc                                           <  2:1350665/150‑1 (MQ=255)
                                                                                                               cTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGccgccg                                      <  2:636590/150‑1 (MQ=255)
                                                                                                                  gCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCgaag                                   <  1:1249645/150‑1 (MQ=255)
                                                                                                                   ccTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCgaaga                                  >  2:1617539/1‑150 (MQ=255)
                                                                                                                     tGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAAc                                >  1:589581/1‑150 (MQ=255)
                                                                                                                        aTACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATg                             <  1:488639/150‑1 (MQ=255)
                                                                                                                              gTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGCACAGGCCGCCGAAGAACATGGCTtct                       >  2:813476/1‑150 (MQ=255)
                                                                                                                                  tttttttCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGtt                   >  2:618766/1‑150 (MQ=255)
                                                                                                                                   ttttttCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGttc                  <  2:208707/150‑1 (MQ=255)
                                                                                                                                   tattttCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCATCAACTCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACAAGGCTTCTCGatc                  >  1:1258989/3‑150 (MQ=255)
                                                                                                                                    tttttCTGATGGTCAACCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGttct                 <  2:1102257/150‑1 (MQ=255)
                                                                                                                                       cgcTGATGGTGATTGACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTcttcc              >  2:224695/3‑150 (MQ=255)
                                                                                                                                       cgcTGATGGTGATTGACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTcttcc              >  1:871715/3‑150 (MQ=255)
                                                                                                                                        tCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTcttcct             <  1:1064589/150‑1 (MQ=255)
                                                                                                                                        gcTGATGGTGATTGACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTcttcct             >  1:655764/2‑150 (MQ=255)
                                                                                                                                        gcTGATGGTGATTGACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTcttcct             >  1:1470156/2‑150 (MQ=255)
                                                                                                                                          tGATGGTCAATCCCCTTCAGCGTTTCCGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGCCAGTTGCGGTTAATCTTCAAATCCCGCCACAAGTTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTcttcattc           >  2:1124886/1‑150 (MQ=255)
                                                                                                                                           gATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCg          <  1:851099/150‑1 (MQ=255)
                                                                                                                                             tGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGt        >  1:1373081/1‑150 (MQ=255)
                                                                                                                                             tGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGt        <  1:1823192/150‑1 (MQ=255)
                                                                                                                                              ggTCAATCTCCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTa       <  1:1144667/150‑1 (MQ=255)
                                                                                                                                              ggTCAATCACCCTCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTa       <  2:1709542/150‑1 (MQ=255)
                                                                                                                                                tCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACa     >  1:1606441/1‑150 (MQ=255)
                                                                                                                                                tCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGGTCTTCCTTCGGTACa     >  1:1093783/1‑150 (MQ=255)
                                                                                                                                                 ctaTCACCATCAGCGTTTCAGCTAGATAAAAACAGAAATCAGTGCAGATATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGATCATGGCTTCTCGTTCTTCCTTCGGTACAt    <  2:1873370/148‑1 (MQ=255)
                                                                                                                                                 cAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCCCAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACAt    >  2:1362957/1‑150 (MQ=255)
                                                                                                                                                  aaTCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACAtt   >  2:885223/1‑150 (MQ=255)
                                                                                                                                                   aTCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTc  <  1:1532353/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGTACATTC  >  NC_000913/1322249‑1322545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑