BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,463,241	G→C	7.9%	V373L (GTG→CTG) ‡	paaK →	phenylacetyl‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,463,241	0	G	C	7.9%	387.7 / 22.4	127	V373L (GTG→CTG) ‡	paaK	phenylacetyl‑CoA ligase
Reads supporting (aligned to +/- strand): ref base G (57/60); new base C (5/5); total (62/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.67e-01

Only 100 of 142 total aligned reads displayed.

GCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAA  >  NC_000913/1463092‑1463390

gCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCtg                                                                                                                                                       >  2:451235/1‑150 (MQ=255)

gCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATAACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCtg                                                                                                                                                       >  1:1672097/1‑150 (MQ=255)

gCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTCATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCtg                                                                                                                                                       <  1:1550758/150‑1 (MQ=255)

gCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTAAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGCGAATCTTGATTCACTTTCtg                                                                                                                                                       >  1:1854390/1‑150 (MQ=255)

 cATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCtgt                                                                                                                                                      <  1:551164/150‑1 (MQ=255)

  aTCAGCGGAAGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCtgtg                                                                                                                                                     <  2:1394755/150‑1 (MQ=255)

    cAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGaa                                                                                                                                                   >  2:10040/1‑150 (MQ=255)

    cAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGaa                                                                                                                                                   <  2:1136815/150‑1 (MQ=255)

        ggACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCACAGAAAGTg                                                                                                                                               <  2:1251827/150‑1 (MQ=255)

          aCGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGa                                                                                                                                             >  2:1415629/1‑150 (MQ=255)

           cGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGTAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAg                                                                                                                                            <  1:497343/150‑1 (MQ=255)

           cGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAg                                                                                                                                            <  2:485348/150‑1 (MQ=255)

            gCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCACAGAAAGTgaatc                                                                                                                                           <  1:1268211/150‑5 (MQ=255)

             cAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGtt                                                                                                                                          >  2:1578940/1‑150 (MQ=255)

              cgCGATGACATGTGGATTATTCGCGGTGTAAATGTCTTTCCGGCACAACTGGAAGAAGAGAGTGTCATATTCGAACATTTATCGCCGCATTCCCAACTGGAGGGGACCCGCCGGGGGCATCTTGATTCACTTTCACAGAAAGTgaatcaa                                                                                                                                         <  1:952014/149‑7 (MQ=255)

               gCGATGACATGTTGTTTATTCGCGGTGTAAATGTCTTTCCGTCACAACTTGAAGAAGAGATTGGCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGTGCATCTTGATTCACTTTCACAGAAAGTgaatcaag                                                                                                                                        <  1:906892/150‑8 (MQ=255)

               gCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAACTGGAGTTga                                                                                                                                        >  1:1561829/1‑150 (MQ=255)

                cGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTgaa                                                                                                                                       <  2:192596/150‑1 (MQ=255)

                     aCATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTgaaagaaa                                                                                                                                  <  2:411245/150‑1 (MQ=255)

                      cATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGaaagaaag                                                                                                                                 >  2:552202/1‑150 (MQ=255)

                      cATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGaaagaaag                                                                                                                                 <  2:1758565/150‑1 (MQ=255)

                      cATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGaaagaaag                                                                                                                                 <  2:874258/150‑1 (MQ=255)

                      aaTGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGaaagaaag                                                                                                                                 <  1:867153/149‑1 (MQ=255)

                            gATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCt                                                                                                                           >  1:685298/1‑150 (MQ=255)

                             attattCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTAAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTg                                                                                                                          <  2:787165/150‑1 (MQ=255)

                              ttattCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGa                                                                                                                         <  2:1451535/150‑1 (MQ=255)

                                attCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACa                                                                                                                       >  1:846412/1‑150 (MQ=255)

                                attCGCGGTGTAAATGTCTTTCAGTAACAACTTGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGTGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGCAAGTAGCCTGACa                                                                                                                       <  1:1068744/150‑1 (MQ=255)

                                 ttCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACAt                                                                                                                      >  1:1244100/1‑150 (MQ=255)

                                     cgGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGAc                                                                                                                  <  2:1839895/150‑1 (MQ=255)

                                      ggTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACg                                                                                                                 <  1:456789/150‑1 (MQ=255)

                                        tgtAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCa                                                                                                               >  1:1583527/1‑150 (MQ=255)

                                          tAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATg                                                                                                             <  2:407183/150‑1 (MQ=255)

                                          tAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATg                                                                                                             <  1:1254781/150‑1 (MQ=255)

                                            aaggTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGag                                                                                                           <  1:798976/147‑1 (MQ=255)

                                             aTGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCTTCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGagc                                                                                                          <  2:874545/150‑1 (MQ=255)

                                                      cGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGcc                                                                                                 >  1:289310/1‑150 (MQ=255)

                                                       gTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCa                                                                                                >  2:933146/1‑150 (MQ=255)

                                                       gTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCa                                                                                                >  1:1622440/1‑150 (MQ=255)

                                                         cacaACTTGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCTACTGGAGGTGAACCGCCGTGGGCATATTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAgg                                                                                              <  1:1103629/150‑1 (MQ=255)

                                                          acaACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGAGAAAGTGGAGTTGAAAGAAAGTCGCCTGACATTGACGCATGAGCAGCGTTGCCAGGt                                                                                             >  1:1153158/1‑150 (MQ=255)

                                                           caACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTa                                                                                            >  1:723191/1‑150 (MQ=255)

                                                             aCTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATg                                                                                          <  2:915098/150‑1 (MQ=255)

                                                             aCTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATg                                                                                          <  2:1001986/150‑1 (MQ=255)

                                                               tGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCCGCGTTGCCAGGTATGcc                                                                                        >  2:1813053/1‑150 (MQ=255)

                                                                 gaagaaGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGctac                                                                                      >  1:1508900/1‑150 (MQ=255)

                                                                   agaagaGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGccacca                                                                                    >  1:601836/1‑150 (MQ=255)

                                                                   agaagaGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGccacca                                                                                    >  1:1241384/1‑150 (MQ=255)

                                                                    gaagaGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAg                                                                                   >  1:389175/1‑150 (MQ=255)

                                                                    gaagaGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAg                                                                                   <  2:460263/150‑1 (MQ=255)

                                                                      agagATTGTCAAATTCGAACATTTATCGCCGCATTACAAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCt                                                                                 >  2:543144/1‑150 (MQ=255)

                                                                           ttGTCAAATTCGAACATTTATCGCCGCATTACCAACTGCAGGTGAACCGCCTGGGGCATATTGATTCACTATCTGTGAAAGTGGAGTTGAAAGCAAGTAGCCTGACATTGACGCATGAGCAGCGTAGCCAGGTATGCCACCAGCAGcccc                                                                            >  1:1011429/1‑150 (MQ=255)

                                                                              tCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATc                                                                         <  1:552202/150‑1 (MQ=255)

                                                                               cAAATTCGAACATTTATCTCCGCATTACCAACTGTAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGGGGAGTTGAAAGAAAGTAGCCTGACATAGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCg                                                                        <  1:98010/150‑1 (MQ=255)

                                                                               cAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCg                                                                        <  1:151532/150‑1 (MQ=255)

                                                                                 aaTTCGAACATTTATCGCCGCATTACCATCTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGa                                                                      <  1:846835/150‑1 (MQ=255)

                                                                                  aTTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGGGTTGCCAGGTATGCCACCAGCTGCGCCATCGGAt                                                                     <  2:762563/150‑1 (MQ=255)

                                                                                     cGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTaa                                                                  >  2:233643/1‑150 (MQ=255)

                                                                                      gAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCAGCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAg                                                                 <  2:78480/150‑1 (MQ=255)

                                                                                       aaCATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGt                                                                <  2:1066669/150‑1 (MQ=255)

                                                                                       aaCATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTCCGCCATCGGATTAAGt                                                                >  1:517439/1‑150 (MQ=255)

                                                                                         cATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCg                                                              >  2:1191804/1‑150 (MQ=255)

                                                                                          aTTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGa                                                             <  1:1578940/150‑1 (MQ=255)

                                                                                                cgccgcATTACCAACTGTAGTTGAACCCCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGAAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGAtggtgg                                                       <  2:1854390/150‑1 (MQ=255)

                                                                                                 gccgcATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTggg                                                      <  2:1404684/150‑1 (MQ=255)

                                                                                                 gccgcATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCAATCGGATTAAGTCGATGGTtgg                                                      >  1:1615341/1‑150 (MQ=255)

                                                                                                     cATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGAtc                                                  >  2:512014/1‑150 (MQ=255)

                                                                                                      aTTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGTGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGAtct                                                 >  2:820774/1‑150 (MQ=255)

                                                                                                        tACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCCTGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGAGTAAGTCGATGGTGGGGATctct                                               >  1:1638442/1‑150 (MQ=255)

                                                                                                            aaCTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGCCGCATGATCAGCGTTGCCAGGTATGCCACCCGCTGCGCCATCGGATTAAGTATATGGTGGGGATCTCTACCg                                           >  1:465181/1‑150 (MQ=255)

                                                                                                            aaCTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCg                                           >  1:1525578/1‑150 (MQ=255)

                                                                                                              cTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGAt                                         >  1:1005743/1‑150 (MQ=255)

                                                                                                               tGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGAtg                                        <  1:1817154/150‑1 (MQ=255)

                                                                                                                 gAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGAtctg                                      >  1:990840/1‑150 (MQ=255)

                                                                                                                      gAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTgatgat                                 >  2:1792790/1‑150 (MQ=255)

                                                                                                                        aCCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCg                               >  1:1301952/1‑150 (MQ=255)

                                                                                                                        aCCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCg                               >  1:1604725/1‑150 (MQ=255)

                                                                                                                        aCCGCCGGGGGCAGCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCg                               <  2:1861238/150‑1 (MQ=255)

                                                                                                                               ggggCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACtg                        <  1:1000085/150‑1 (MQ=255)

                                                                                                                                gggCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGAAGCGTTGCCAGGTATGCAACCAGCTGCGCCATAGGATGAAGTCGATGGTGGGGATCTCTACCGAAGTCATGATCGTTAAAtgt                       >  1:231387/1‑150 (MQ=255)

                                                                                                                                   cATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCTTCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGc                    <  1:477061/150‑1 (MQ=255)

                                                                                                                                   cATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGc                    <  2:765760/150‑1 (MQ=255)

                                                                                                                                     tCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAg                  >  2:1040025/1‑150 (MQ=255)

                                                                                                                                        tGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTAt               >  2:1449958/1‑150 (MQ=255)

                                                                                                                                         actccACTTTCACAGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATc              >  2:1674700/5‑150 (MQ=255)

                                                                                                                                          ctccACTTTCACAGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATcc             >  2:590763/4‑150 (MQ=255)

                                                                                                                                          ctccACTTTCACAGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATcc             >  2:598584/4‑150 (MQ=255)

                                                                                                                                           ttCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTATGTGGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATccc            >  2:1148443/1‑150 (MQ=255)

                                                                                                                                           ttCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATccc            >  2:696286/1‑150 (MQ=255)

                                                                                                                                             cACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGCCGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCcgc          <  2:685298/150‑1 (MQ=255)

                                                                                                                                             cACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCcgc          >  2:59427/1‑150 (MQ=255)

                                                                                                                                              aCTTTTTGTGAAAGTGGAGTTTAAAGAAAGTAGCCTTACTTTGACGAATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCcgcg         <  2:1241384/150‑1 (MQ=255)

                                                                                                                                               atttCTGTGAAAGTGGAGGTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGt        <  1:475783/149‑1 (MQ=255)

                                                                                                                                                   cTGTGAAAGTGGAGTTGACAGAAACTCGCCTGACTTTTAGGCTTGAGCAGCGTTGCCAGGTATGCCACCAGCAGCGCCATCGGATTAATTCGATGGTGGGGATCTCTTCCGATGTGATGCTCGTTAACTGGGGCAGTATCCCGCGTTCAg    <  1:139571/150‑1 (MQ=255)

                                                                                                                                                   cTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGCCATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAg    >  2:1553123/1‑150 (MQ=255)

                                                                                                                                                    tgtgAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGa   >  1:803055/1‑150 (MQ=255)

                                                                                                                                                    tgtgAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGa   >  1:924050/1‑150 (MQ=255)

                                                                                                                                                    tgtgAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGa   >  1:1790262/1‑150 (MQ=255)

                                                                                                                                                     gtgAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGaa  >  2:500454/1‑150 (MQ=255)

                                                                                                                                                     gtgAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGAGGCAGTATCCCGCGTTCAGaa  >  2:1658295/1‑150 (MQ=255)

GCATCAGCGGACGCAGCGATGACATGTTGATTATTCGCGGTGTAAATGTCTTTCCGTCACAACTGGAAGAAGAGATTGTCAAATTCGAACATTTATCGCCGCATTACCAACTGGAGGTGAACCGCCGGGGGCATCTTGATTCACTTTCTGTGAAAGTGGAGTTGAAAGAAAGTAGCCTGACATTGACGCATGAGCAGCGTTGCCAGGTATGCCACCAGCTGCGCCATCGGATTAAGTCGATGGTGGGGATCTCTACCGATGTGATGATCGTTAACTGTGGCAGTATCCCGCGTTCAGAA  >  NC_000913/1463092‑1463390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`