Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,471,102 G→C 5.4% pseudogene (586/3497 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,471,1020GC 5.4% 287.6 / 7.6 93pseudogene (586/3497 nt)ydbApseudogene, autotransporter homolog; interrupted by IS2 and IS30
Reads supporting (aligned to +/- strand):  ref base G (48/40);  new base C (3/2);  total (51/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.10e-01

ATAGCTAGCAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTTATCAATATCA  >  NC_000913/1470954‑1471251
                                                                                                                                                    |                                                                                                                                                     
ataGCTAGCAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGgc                                                                                                                                                      <  1:1703482/150‑1 (MQ=255)
 tagctagcAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGgcg                                                                                                                                                     >  2:758898/1‑150 (MQ=255)
   gctagcAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGcc                                                                                                                                                   >  1:1320195/1‑150 (MQ=255)
    ctagcAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCa                                                                                                                                                  <  1:912709/150‑1 (MQ=255)
     tagcAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAg                                                                                                                                                 <  1:1093644/150‑1 (MQ=255)
     tagcAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGGAGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAg                                                                                                                                                 <  2:367496/150‑1 (MQ=255)
       gcAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGCCATAGGCCGCCatac                                                                                                                                               <  1:784316/150‑4 (MQ=255)
        cAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCaa                                                                                                                                              >  2:421205/1‑150 (MQ=255)
        cAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCaa                                                                                                                                              >  2:1649796/1‑150 (MQ=255)
         aaCAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGTAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAAt                                                                                                                                             <  1:811900/150‑1 (MQ=255)
          aCAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTTTAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCTCCAGCAATa                                                                                                                                            >  1:61597/1‑150 (MQ=255)
          aCAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGCCATAGGCCGCCatacctg                                                                                                                                            <  1:1265118/150‑7 (MQ=255)
           catcgcCATTGACTTCGGTACACGTGTGGGAGTTTGTACTGATAGTTATTGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGGTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATAc                                                                                                                                           <  2:704261/147‑1 (MQ=255)
           cAGCGCCATTGACTTCGGTACAGGTGTGGGTGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCCGCAATAc                                                                                                                                           >  2:1054084/1‑150 (MQ=255)
            aGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACa                                                                                                                                          >  2:749182/1‑150 (MQ=255)
             gcgcCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAg                                                                                                                                         >  1:1728654/1‑150 (MQ=255)
             gcgcCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAg                                                                                                                                         <  1:1282131/150‑1 (MQ=255)
                       cTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCa                                                                                                                               >  1:1087601/1‑150 (MQ=255)
                        ttCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAg                                                                                                                              <  2:562591/150‑1 (MQ=255)
                         tCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAgg                                                                                                                             >  1:1170552/1‑150 (MQ=255)
                               cAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGa                                                                                                                       >  2:1697313/1‑150 (MQ=255)
                               cAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGa                                                                                                                       >  2:90072/1‑150 (MQ=255)
                                aGGTGTGGGAGTTTGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGAt                                                                                                                      <  1:78511/150‑1 (MQ=255)
                                 ggTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGagt                                                                                                                     >  1:1405264/1‑148 (MQ=255)
                                 ggTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAATGCAACAGCAATTAAGCAATTGGGCGGTGTTATAACTATTTATAACGCATGCGAAGGTATGGCGGCCTATGGCGCCAGCTATACAGTTATTATCCCGGGGACGATTa                                                                                                                    >  2:1154544/1‑150 (MQ=255)
                                   tgtgGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTaa                                                                                                                   >  1:833451/1‑150 (MQ=255)
                                     tgGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGGGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAAcc                                                                                                                 <  2:868533/150‑1 (MQ=255)
                                     ggggAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAAcc                                                                                                                 <  1:522613/149‑1 (MQ=255)
                                      gggAGTTGGTACTGATAGTTATAGTGGTGCAGGGTAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCt                                                                                                                <  1:831815/150‑1 (MQ=255)
                                        gAGTTGGTACTGATAGTTATAGTGGTGCAGTGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCg                                                                                                              <  2:1663340/150‑1 (MQ=255)
                                           ttGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGaaa                                                                                                           >  1:1849945/1‑150 (MQ=255)
                                            tGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTAATTAACCAGGGGACGATTAACCTCGaaaa                                                                                                          >  1:400026/1‑150 (MQ=255)
                                            tGGTACTGATAGATATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCCCAGGTATGGCGGCATATGGCGCAAGCAATACAGAAATTAACCAGGGGACGATTACCCTCGaaaa                                                                                                          >  1:342079/1‑150 (MQ=255)
                                             ggTACTGATAGTAATAGTCGTGCAGGGAAAAATGCAACACCAATTAACCAAGTGGGCGGTGTTTTAACTATTAATAACGCCGGCGCAGGTATGGAGGCCTATGGCGCCAGCAAAACAGTTAATAACCAGGGGACGATTACCCTCGaaaaa                                                                                                         >  2:978563/1‑150 (MQ=255)
                                               tACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGaaaaaaa                                                                                                       >  1:1527738/1‑150 (MQ=255)
                                                aCTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAAt                                                                                                      >  1:1228292/1‑150 (MQ=255)
                                                aCTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAAt                                                                                                      <  1:614001/150‑1 (MQ=255)
                                                       gTTATAGTGGTTCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAAtt                                                                                               <  1:1432987/150‑1 (MQ=255)
                                                       gTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAAtt                                                                                               <  1:1563834/150‑1 (MQ=255)
                                                           aaGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGa                                                                                           <  1:1762468/149‑1 (MQ=255)
                                                                gtgCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCTGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAAGCAGGGGACGATTAACCTCGAAAATAATGGTAATTATGAAGAtt                                                                                      >  2:382085/1‑149 (MQ=255)
                                                                gtgCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATa                                                                                      >  1:889198/1‑150 (MQ=255)
                                                                  gCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGt                                                                                    >  1:1175359/1‑150 (MQ=255)
                                                                       gAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGgc                                                                               >  1:1146703/1‑150 (MQ=255)
                                                                       gAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGgc                                                                               <  1:90072/150‑1 (MQ=255)
                                                                         aaaaTGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGgcag                                                                             <  1:1581605/150‑1 (MQ=255)
                                                                               cAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATa                                                                       >  2:704257/1‑150 (MQ=255)
                                                                               cAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATa                                                                       >  2:439578/1‑150 (MQ=255)
                                                                                 aCAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATAca                                                                     >  1:797835/1‑149 (MQ=255)
                                                                                  cAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTATTTATGACGATAGTCTGGCAGCAAATActc                                                                    >  2:892053/1‑150 (MQ=255)
                                                                                  cAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATActc                                                                    >  1:60441/1‑150 (MQ=255)
                                                                                  cAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATAct                                                                     <  2:1495947/149‑1 (MQ=255)
                                                                                  cAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATAct                                                                     >  1:1495947/1‑149 (MQ=255)
                                                                                   aGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATActct                                                                   >  2:696465/1‑150 (MQ=255)
                                                                                    gCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTg                                                                  >  2:1676489/1‑150 (MQ=255)
                                                                                       aTTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTATTTATGACGATAGTCTGGCAGCAAATACTCTGGTa                                                               >  2:980246/1‑150 (MQ=255)
                                                                                          aaCCAATTGGGCGGTGTTATAACTATTTATACCGCCGGAGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGGTATTACCCAGGGGACGATTTCCCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAggg                                                            >  2:1638567/1‑150 (MQ=255)
                                                                                          aaCCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAggg                                                            <  1:1837308/150‑1 (MQ=255)
                                                                                          aaCCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAggg                                                            >  2:1829087/1‑150 (MQ=255)
                                                                                          aaCCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAggg                                                            >  1:1504144/1‑150 (MQ=255)
                                                                                          aaCCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATAAAGTTATTAACCAGGGGACGATTACCCACGAATAAAATGGTAATAATGACGATAGTCTTGCAGCAAATAGTCTGGTAggg                                                            >  2:155879/1‑150 (MQ=255)
                                                                                          aaCCAATTCGGCGGTGTTTTAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAATAAATGGTAATTATGACGATAGTCTGCAAGCAAATACTCTGGTAggg                                                            >  2:826957/1‑150 (MQ=255)
                                                                                           aCCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGa                                                           <  1:1690426/150‑1 (MQ=255)
                                                                                           aCCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGa                                                           <  2:1042843/150‑1 (MQ=255)
                                                                                           aCCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGAt                                                          <  1:1386063/150‑1 (MQ=255)
                                                                                            ccAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGAt                                                          >  2:1639067/1‑150 (MQ=255)
                                                                                             cAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATg                                                         >  1:955831/1‑150 (MQ=255)
                                                                                             cAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATg                                                         >  2:1163071/1‑150 (MQ=255)
                                                                                               aTTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGc                                                       <  2:1469806/150‑1 (MQ=255)
                                                                                                 tGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTg                                                     >  2:490649/1‑150 (MQ=255)
                                                                                                  gggCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGt                                                    <  2:1788430/150‑1 (MQ=255)
                                                                                                       gtgtTTTAACTATTTATAACGCCGGGGCTGGTATGGCGTACTATGGCGCCAGCAATACAGTTATTAAGCAGGTGACGATTAACCTCGAATAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTTGGGATGGCTGTTTATg                                               <  2:980947/150‑1 (MQ=255)
                                                                                                         tttATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAg                                             <  1:779305/149‑1 (MQ=255)
                                                                                                         gtTATAACTATTTATAACTCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAg                                             <  1:1697313/150‑1 (MQ=255)
                                                                                                         gtTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAg                                             <  1:664176/150‑1 (MQ=255)
                                                                                                           tataACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCa                                           <  1:1649796/150‑1 (MQ=255)
                                                                                                                       taACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTATTTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTAt                               >  1:1347915/1‑150 (MQ=255)
                                                                                                                        aaCGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATc                              <  2:1728654/150‑1 (MQ=255)
                                                                                                                         aCGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCTTGGTACTGCTATCa                             >  1:1028046/1‑150 (MQ=255)
                                                                                                                          cGCCTGCTCAGGTATGGCGGCCTATGGCGGCAGCAATTCAGTTATTACCCAGGGGACGATTAACATCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGAAAATACTCTTGTAGTGATGTCTGTTTATGAGCATGGTACTGCTATaaa                            <  1:1154544/150‑1 (MQ=255)
                                                                                                                            ccGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACg                          <  1:47156/150‑1 (MQ=255)
                                                                                                                              ggCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGAc                        <  2:1452853/150‑1 (MQ=255)
                                                                                                                              ggCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGAc                        >  2:1357934/1‑150 (MQ=255)
                                                                                                                                 gcAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAATAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAg                     <  1:749182/150‑1 (MQ=255)
                                                                                                                                   attgctGGCGCCATAGGCCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGAc                   >  1:509162/6‑150 (MQ=255)
                                                                                                                                    ttgctGGCGCCATAGGCCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGAAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACg                  >  2:1547402/5‑150 (MQ=255)
                                                                                                                                    ggTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACg                  >  1:378974/1‑150 (MQ=255)
                                                                                                                                      gctGGCGCCATAGGCCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACggg                >  1:1672625/3‑150 (MQ=255)
                                                                                                                                        tGGCGGCCTATGGAGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGgtg              <  1:421205/150‑1 (MQ=255)
                                                                                                                                         ggcggcCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGgtgt             >  2:822460/1‑150 (MQ=255)
                                                                                                                                         ggcggcCTATGGCGCCAGAAATACAGTTATTAACCAGGGGACTATTAACCTCGAAAAAAATTGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGgtgt             <  2:61597/150‑1 (MQ=255)
                                                                                                                                            ggcCTTTTGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGGGTtat          <  1:1054084/150‑1 (MQ=255)
                                                                                                                                            ggcCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTtat          >  1:860480/1‑150 (MQ=255)
                                                                                                                                              ccTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTtatca        <  2:860456/150‑1 (MQ=255)
                                                                                                                                                tATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTtatcaat      <  2:1087601/150‑1 (MQ=255)
                                                                                                                                                    gcgcCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTtatcaatatca  >  2:1299255/1‑150 (MQ=255)
                                                                                                                                                    gcgcCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTtatcaatatca  <  1:1370005/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATAGCTAGCAACAGCGCCATTGACTTCGGTACAGGTGTGGGAGTTGGTACTGATAGTTATAGTGGTGCAGGGAAAAATGCAACAGCAATTAACCAATTGGGCGGTGTTATAACTATTTATAACGCCGGCGCAGGTATGGCGGCCTATGGCGCCAGCAATACAGTTATTAACCAGGGGACGATTAACCTCGAAAAAAATGGTAATTATGACGATAGTCTGGCAGCAAATACTCTGGTAGGGATGGCTGTTTATGAGCATGGTACTGCTATCAACGACCAGACGGGTGTTATCAATATCA  >  NC_000913/1470954‑1471251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: