Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,481,117 T→A 5.3% V70D (GTT→GAT) ‡ ynbD → putative phosphatase inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,481,1170TA 5.3% 270.4 / 5.1 95V70D (GTT→GAT) ‡ynbDputative phosphatase inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (43/47);  new base A (3/2);  total (46/49)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.39e-01

ATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCA  >  NC_000913/1480968‑1481264
                                                                                                                                                     |                                                                                                                                                   
aTCTCTTAATCAGTTCACCGAGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGTATTTTCGCTGTTCGt                                                                                                                                                     >  1:99162/1‑150 (MQ=255)
   tctTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAAAGGCTATCCCTTTTCTTCCCTGGACTATTGTTCCTTTCTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTg                                                                                                                                                  <  1:313671/150‑1 (MQ=255)
   tctTAATCAGTTCACCGCGGTTCAGGAACTTAACAGCCATGATATAACCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCGTTTCTTCCCTGGACTATTGTTCCTTACAGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTg                                                                                                                                                  >  2:293050/1‑150 (MQ=255)
    ctTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCTCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGt                                                                                                                                                 >  1:682375/1‑150 (MQ=255)
      tAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAg                                                                                                                                               >  2:1093849/1‑150 (MQ=255)
          cAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACg                                                                                                                                           >  1:1409149/1‑150 (MQ=255)
            gTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCACTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGAc                                                                                                                                         <  1:1525860/150‑1 (MQ=255)
               cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGATACGGCGAGCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACAtt                                                                                                                                      <  1:1103653/150‑1 (MQ=255)
               cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGCTCCATTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACAtt                                                                                                                                      >  1:96428/1‑150 (MQ=255)
               cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACAtt                                                                                                                                      >  1:852629/1‑150 (MQ=255)
                aCCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTCGACTATTGTTCCTTACTGGAGTCTGGATCGTTTATATTGATTTTCGCTGTTCGTATGTAGCACGACAtta                                                                                                                                     >  1:19560/1‑149 (MQ=255)
                 ccGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCg                                                                                                                                    >  1:1141152/1‑150 (MQ=255)
                 ccGCGGTTCAGGACCTTAACAGCCATGATATCACCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTAGCTGGACTATTGTTACTTACTGGAGTCTGGATCTTTTATATTGATTTTCGCTGTTCGTTTGTAGCACGACATTCg                                                                                                                                    >  2:1540920/1‑150 (MQ=255)
                  cgcgCTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGACCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGa                                                                                                                                   <  2:268707/150‑1 (MQ=255)
                    cgGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTTGGAATCGGCGATCCCTTTTCTTCCCTTGACTATTGTTCCTTACTTGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAAc                                                                                                                                 <  2:873342/150‑1 (MQ=255)
                        tCAGGACCTTAACAGCCATGATATCCCCAGTCTGGTCTTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCGGGACTATTGGTCCTTCCTGGAGTCTGGAGCTTTTATATGGATTTTCGCTGTTCGAATGTCGTGCTACAAACGAACAgcg                                                                                                                             <  1:1409704/150‑1 (MQ=255)
                           ggACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCg                                                                                                                          <  2:58138/150‑1 (MQ=255)
                           ggACCTTAACAGCCATGAAATCCCCAGGCAGGTATTCGGGGGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCg                                                                                                                          <  1:1193864/150‑1 (MQ=255)
                            gACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGAATGTCGTGCTACAAACGAACAgcgaaaa                                                                                                                         <  1:749708/150‑5 (MQ=38)
                             aCCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGAc                                                                                                                        >  2:868675/1‑150 (MQ=255)
                              ccTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACt                                                                                                                       >  1:799047/1‑150 (MQ=255)
                              ccTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACt                                                                                                                       <  1:1657105/150‑1 (MQ=255)
                                  aaCAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTc                                                                                                                   <  2:1786428/150‑1 (MQ=255)
                                     aGCCATGATATCCCCAGTCAGGTTTTCGTTGGGGAAACTGCGATCCCTTTTCTTCCCTGGTCTATTGTTACTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCAc                                                                                                                <  1:225006/150‑1 (MQ=255)
                                      gCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTTTTGTTCCTTACGGGAGTCTGGTTCTTTTAGTTGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGCTCAACGCCGTCTTGTCGAcc                                                                                                               >  2:1546683/1‑150 (MQ=255)
                                      gCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCAcc                                                                                                               >  1:1675920/1‑150 (MQ=255)
                                       ccATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCg                                                                                                              >  1:287586/1‑150 (MQ=255)
                                        cATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCgg                                                                                                             >  1:1090873/1‑150 (MQ=255)
                                          tGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCt                                                                                                           <  1:1728800/150‑1 (MQ=255)
                                           gATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCtt                                                                                                          <  1:1091924/150‑1 (MQ=255)
                                             tatCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTAt                                                                                                        <  1:947012/150‑1 (MQ=255)
                                             tatCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTGCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTAt                                                                                                        <  2:1124916/150‑1 (MQ=255)
                                               tCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTc                                                                                                      >  1:1683629/1‑150 (MQ=255)
                                                 cccAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTg                                                                                                    >  1:1465841/1‑150 (MQ=255)
                                                  ccAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTgg                                                                                                   <  2:442353/150‑1 (MQ=255)
                                                   cAGTCAGGTATTCGGTTGGGAAACGGCTATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGAGTTGTCCACCGGCTTATTCTGGc                                                                                                  <  1:524697/150‑1 (MQ=255)
                                                   cAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGc                                                                                                  >  1:974145/1‑150 (MQ=255)
                                                        aGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACAGGCTTATTCTGGCAACgg                                                                                             >  1:364001/1‑150 (MQ=255)
                                                              tCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGAATTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATgg                                                                                       <  2:880355/150‑1 (MQ=255)
                                                                  ttGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctg                                                                                   <  1:1352089/150‑1 (MQ=255)
                                                                  ttGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTAGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctg                                                                                   <  2:1625579/150‑1 (MQ=255)
                                                                   tGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctgc                                                                                  >  1:996993/1‑150 (MQ=255)
                                                                    gggAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctgct                                                                                 >  1:701572/1‑150 (MQ=255)
                                                                     ggAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTGGTCCACCGGCGTATTCTGGCAACGGTAATGGCctgcag                                                                                >  2:1649551/1‑148 (MQ=255)
                                                                      gAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTTTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGGAATGGCCtgctgc                                                                               >  2:498230/1‑150 (MQ=255)
                                                                        aaCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCgg                                                                             <  1:1492411/150‑1 (MQ=255)
                                                                          cGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtt                                                                           <  2:788225/150‑1 (MQ=255)
                                                                             cGATCCCTTTTCTTCCCTGGCCTATTGTTCCTTCCTGGAGTCTGGATCTTATATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                        <  2:1767488/150‑1 (MQ=255)
                                                                             cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGCGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGAAGCACGACATTCGAACAGCGCCGACTTGGCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTTCGGttttt                                                                        <  2:140779/150‑1 (MQ=255)
                                                                             cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                        >  2:161196/1‑150 (MQ=255)
                                                                             cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                        <  2:1211895/150‑1 (MQ=255)
                                                                             cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                        >  1:1111168/1‑150 (MQ=255)
                                                                              tataccTTTTCTTCCCTGTACTATTGTTCGTTAGTGGTGTCTGTATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACTACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATTGCCTGCTGCGGtttttt                                                                       <  1:1082754/146‑1 (MQ=255)
                                                                              gATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtttttt                                                                       <  1:1631753/150‑1 (MQ=255)
                                                                              gATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtttttt                                                                       <  1:930470/150‑1 (MQ=255)
                                                                              gATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTGCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtttttt                                                                       <  1:70589/150‑1 (MQ=255)
                                                                               aTCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTTGATCTTTTATATGGATTTTCGCTGATCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTg                                                                      <  1:293050/150‑1 (MQ=255)
                                                                                    ttttCTTCCCTGGACTATTGTTACTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTa                                                                 <  2:96428/150‑1 (MQ=255)
                                                                                       tcttcCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATGCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATcc                                                              <  1:1294167/150‑1 (MQ=255)
                                                                                          tcCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCAACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCt                                                           >  1:1661529/1‑150 (MQ=255)
                                                                                           cccTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGGGGTGTTTTGCTCTATCCGCTg                                                          >  2:1874869/1‑150 (MQ=255)
                                                                                           cccTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTg                                                          >  1:1544954/1‑150 (MQ=255)
                                                                                           cccTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTg                                                          <  2:546742/150‑1 (MQ=255)
                                                                                               ggACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAagt                                                      <  1:1093849/150‑1 (MQ=255)
                                                                                                       gTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGtttt                                              >  1:1617839/1‑150 (MQ=255)
                                                                                                          ccTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATc                                           <  2:737146/150‑1 (MQ=255)
                                                                                                              aCTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTc                                       >  2:1552729/1‑150 (MQ=255)
                                                                                                               cTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTcc                                      <  2:682375/150‑1 (MQ=255)
                                                                                                               cTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTcc                                      <  2:1683629/150‑1 (MQ=255)
                                                                                                                 ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                    >  1:1466370/1‑150 (MQ=255)
                                                                                                                 ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                    >  2:727470/1‑150 (MQ=255)
                                                                                                                 ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                    >  2:708747/1‑150 (MQ=255)
                                                                                                                 ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                    >  2:708355/1‑150 (MQ=255)
                                                                                                                 ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                    >  1:989561/1‑150 (MQ=255)
                                                                                                                     tCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAagt                                <  1:494055/150‑1 (MQ=255)
                                                                                                                       tGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAagtga                              >  1:1829618/1‑150 (MQ=255)
                                                                                                                       tGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAagtga                              >  1:9518/1‑150 (MQ=255)
                                                                                                                            cTTTTATATGGAGTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTTATGGCCTGCTGCGGTTGTTTGCTCTATCCGCTGAAGTTTAGTTTTATCGGTCCTGAAGTGAGTggg                         >  2:113209/1‑150 (MQ=255)
                                                                                                                              tttATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGt                       >  1:417055/1‑150 (MQ=255)
                                                                                                                                  tatGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACg                   >  2:1440269/1‑150 (MQ=255)
                                                                                                                                   atGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACgg                  >  1:174094/1‑150 (MQ=255)
                                                                                                                                     ggATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGGCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGa                <  1:479348/150‑1 (MQ=255)
                                                                                                                                       aTTTTCGCTGTTCGTTTGTAGCACGACATTCGAAAAGCGCCGACTTTTCAACCGGCTTATTATGGCAACGTTAATTGACTGCTGCGGTTTTTTGATCTATCAGCTGAAGTTTAGTTTTATCAGTCCTGAAGTGAGTGTGGTGACGGGTTg              >  1:1310248/1‑150 (MQ=255)
                                                                                                                                        tgttCGCTGTGCGTTTGAAGCACGACATTCGAACAGCGCCGACTTGCCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTCTCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATgg             <  1:296185/148‑1 (MQ=255)
                                                                                                                                         tttCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGc            <  2:417055/150‑1 (MQ=255)
                                                                                                                                         tttCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTGGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGc            <  1:279937/150‑1 (MQ=255)
                                                                                                                                          ggcGCTGTTCGAATGTCGTGCTACAAACGAACAGCGCCGCCTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCTCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGCCGGGATGGCt           >  2:1075390/3‑150 (MQ=37)
                                                                                                                                           gcGCTGTTCGAATGTCGTGCTACAAACGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTa          >  1:1655546/2‑150 (MQ=255)
                                                                                                                                           gcGCTGTTCGAATGTCGTGCTACAAACGAACAGCGCAGACTTGTCCGCCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCGTGAAGTGAGTGGGGTGACGGGATGGCTa          >  1:1843250/2‑150 (MQ=255)
                                                                                                                                            cGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCTGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTAt         <  2:852629/150‑1 (MQ=255)
                                                                                                                                              cTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTAttt       <  1:161196/150‑1 (MQ=255)
                                                                                                                                                gTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCTGCTTATTATTGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATACGTCCTGAAGTGAGTGGGGTGACGGGATGGATATTTTc     >  1:1697642/1‑150 (MQ=255)
                                                                                                                                                gTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTc     <  2:1465841/150‑1 (MQ=255)
                                                                                                                                                 ttCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGTGTGACGGGATGGCTATTTTCg    <  1:1649551/150‑1 (MQ=255)
                                                                                                                                                  tCGTTTGTAGCACGACATTCGTACAGCGCCGAGTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCAGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGc   <  2:1799797/150‑1 (MQ=255)
                                                                                                                                                   cGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCa  <  2:287586/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCA  >  NC_000913/1480968‑1481264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: