Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,481,118 T→A 5.9% V70V (GTT→GTA) ‡ ynbD → putative phosphatase inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,481,1180TA 5.9% 269.7 / 5.2 95V70V (GTT→GTA) ‡ynbDputative phosphatase inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (42/47);  new base A (4/2);  total (46/49)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

TCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCA  >  NC_000913/1480971‑1481264
                                                                                                                                                   |                                                                                                                                                  
tctTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAAAGGCTATCCCTTTTCTTCCCTGGACTATTGTTCCTTTCTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTg                                                                                                                                                  <  1:313671/150‑1 (MQ=255)
tctTAATCAGTTCACCGCGGTTCAGGAACTTAACAGCCATGATATAACCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCGTTTCTTCCCTGGACTATTGTTCCTTACAGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTg                                                                                                                                                  >  2:293050/1‑150 (MQ=255)
 ctTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCTCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGt                                                                                                                                                 >  1:682375/1‑150 (MQ=255)
   tAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAg                                                                                                                                               >  2:1093849/1‑150 (MQ=255)
       cAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACg                                                                                                                                           >  1:1409149/1‑150 (MQ=255)
         gTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCACTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGAc                                                                                                                                         <  1:1525860/150‑1 (MQ=255)
            cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGATACGGCGAGCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACAtt                                                                                                                                      <  1:1103653/150‑1 (MQ=255)
            cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGCTCCATTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACAtt                                                                                                                                      >  1:96428/1‑150 (MQ=255)
            cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACAtt                                                                                                                                      >  1:852629/1‑150 (MQ=255)
             aCCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTCGACTATTGTTCCTTACTGGAGTCTGGATCGTTTATATTGATTTTCGCTGTTCGTATGTAGCACGACAtta                                                                                                                                     >  1:19560/1‑149 (MQ=255)
              ccGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCg                                                                                                                                    >  1:1141152/1‑150 (MQ=255)
              ccGCGGTTCAGGACCTTAACAGCCATGATATCACCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTAGCTGGACTATTGTTACTTACTGGAGTCTGGATCTTTTATATTGATTTTCGCTGTTCGTTTGTAGCACGACATTCg                                                                                                                                    >  2:1540920/1‑150 (MQ=255)
               cgcgCTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGACCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGa                                                                                                                                   <  2:268707/150‑1 (MQ=255)
                 cgGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTTGGAATCGGCGATCCCTTTTCTTCCCTTGACTATTGTTCCTTACTTGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAAc                                                                                                                                 <  2:873342/150‑1 (MQ=255)
                     tCAGGACCTTAACAGCCATGATATCCCCAGTCTGGTCTTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCGGGACTATTGGTCCTTCCTGGAGTCTGGAGCTTTTATATGGATTTTCGCTGTTCGAATGTCGTGCTACAAACGAACAgcg                                                                                                                             <  1:1409704/150‑1 (MQ=255)
                        ggACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCg                                                                                                                          <  2:58138/150‑1 (MQ=255)
                        ggACCTTAACAGCCATGAAATCCCCAGGCAGGTATTCGGGGGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCg                                                                                                                          <  1:1193864/150‑1 (MQ=255)
                         gACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGAATGTCGTGCTACAAACGAACAgcgaaaa                                                                                                                         <  1:749708/150‑5 (MQ=38)
                          aCCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGAc                                                                                                                        >  2:868675/1‑150 (MQ=255)
                           ccTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACt                                                                                                                       >  1:799047/1‑150 (MQ=255)
                           ccTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACt                                                                                                                       <  1:1657105/150‑1 (MQ=255)
                               aaCAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTc                                                                                                                   <  2:1786428/150‑1 (MQ=255)
                                  aGCCATGATATCCCCAGTCAGGTTTTCGTTGGGGAAACTGCGATCCCTTTTCTTCCCTGGTCTATTGTTACTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCAc                                                                                                                <  1:225006/150‑1 (MQ=255)
                                   gCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTTTTGTTCCTTACGGGAGTCTGGTTCTTTTAGTTGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGCTCAACGCCGTCTTGTCGAcc                                                                                                               >  2:1546683/1‑150 (MQ=255)
                                   gCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCAcc                                                                                                               >  1:1675920/1‑150 (MQ=255)
                                    ccATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCg                                                                                                              >  1:287586/1‑150 (MQ=255)
                                     cATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCgg                                                                                                             >  1:1090873/1‑150 (MQ=255)
                                       tGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCt                                                                                                           <  1:1728800/150‑1 (MQ=255)
                                        gATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCtt                                                                                                          <  1:1091924/150‑1 (MQ=255)
                                          tatCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTAt                                                                                                        <  1:947012/150‑1 (MQ=255)
                                          tatCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTGCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTAt                                                                                                        <  2:1124916/150‑1 (MQ=255)
                                            tCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTc                                                                                                      >  1:1683629/1‑150 (MQ=255)
                                              cccAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTg                                                                                                    >  1:1465841/1‑150 (MQ=255)
                                               ccAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTgg                                                                                                   <  2:442353/150‑1 (MQ=255)
                                                cAGTCAGGTATTCGGTTGGGAAACGGCTATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGAGTTGTCCACCGGCTTATTCTGGc                                                                                                  <  1:524697/150‑1 (MQ=255)
                                                cAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGc                                                                                                  >  1:974145/1‑150 (MQ=255)
                                                     aGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACAGGCTTATTCTGGCAACgg                                                                                             >  1:364001/1‑150 (MQ=255)
                                                           tCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGAATTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATgg                                                                                       <  2:880355/150‑1 (MQ=255)
                                                               ttGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctg                                                                                   <  1:1352089/150‑1 (MQ=255)
                                                               ttGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTAGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctg                                                                                   <  2:1625579/150‑1 (MQ=255)
                                                                tGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctgc                                                                                  >  1:996993/1‑150 (MQ=255)
                                                                 gggAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctgct                                                                                 >  1:701572/1‑150 (MQ=255)
                                                                  ggAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTGGTCCACCGGCGTATTCTGGCAACGGTAATGGCctgcag                                                                                >  2:1649551/1‑148 (MQ=255)
                                                                   gAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTTTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGGAATGGCCtgctgc                                                                               >  2:498230/1‑150 (MQ=255)
                                                                     aaCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCgg                                                                             <  1:1492411/150‑1 (MQ=255)
                                                                       cGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtt                                                                           <  2:788225/150‑1 (MQ=255)
                                                                          cGATCCCTTTTCTTCCCTGGCCTATTGTTCCTTCCTGGAGTCTGGATCTTATATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                        <  2:1767488/150‑1 (MQ=255)
                                                                          cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGCGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGAAGCACGACATTCGAACAGCGCCGACTTGGCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTTCGGttttt                                                                        <  2:140779/150‑1 (MQ=255)
                                                                          cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                        <  2:1211895/150‑1 (MQ=255)
                                                                          cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                        >  2:161196/1‑150 (MQ=255)
                                                                          cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                        >  1:1111168/1‑150 (MQ=255)
                                                                           tataccTTTTCTTCCCTGTACTATTGTTCGTTAGTGGTGTCTGTATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACTACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATTGCCTGCTGCGGtttttt                                                                       <  1:1082754/146‑1 (MQ=255)
                                                                           gATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtttttt                                                                       <  1:930470/150‑1 (MQ=255)
                                                                           gATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtttttt                                                                       <  1:1631753/150‑1 (MQ=255)
                                                                           gATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTGCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtttttt                                                                       <  1:70589/150‑1 (MQ=255)
                                                                            aTCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTTGATCTTTTATATGGATTTTCGCTGATCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTg                                                                      <  1:293050/150‑1 (MQ=255)
                                                                                 ttttCTTCCCTGGACTATTGTTACTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTa                                                                 <  2:96428/150‑1 (MQ=255)
                                                                                    tcttcCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATGCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATcc                                                              <  1:1294167/150‑1 (MQ=255)
                                                                                       tcCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCAACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCt                                                           >  1:1661529/1‑150 (MQ=255)
                                                                                        cccTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGGGGTGTTTTGCTCTATCCGCTg                                                          >  2:1874869/1‑150 (MQ=255)
                                                                                        cccTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTg                                                          <  2:546742/150‑1 (MQ=255)
                                                                                        cccTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTg                                                          >  1:1544954/1‑150 (MQ=255)
                                                                                            ggACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAagt                                                      <  1:1093849/150‑1 (MQ=255)
                                                                                                    gTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGtttt                                              >  1:1617839/1‑150 (MQ=255)
                                                                                                       ccTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATc                                           <  2:737146/150‑1 (MQ=255)
                                                                                                           aCTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTc                                       >  2:1552729/1‑150 (MQ=255)
                                                                                                            cTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTcc                                      <  2:682375/150‑1 (MQ=255)
                                                                                                            cTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTcc                                      <  2:1683629/150‑1 (MQ=255)
                                                                                                              ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                    >  1:1466370/1‑150 (MQ=255)
                                                                                                              ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                    >  2:727470/1‑150 (MQ=255)
                                                                                                              ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                    >  2:708747/1‑150 (MQ=255)
                                                                                                              ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                    >  2:708355/1‑150 (MQ=255)
                                                                                                              ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                    >  1:989561/1‑150 (MQ=255)
                                                                                                                  tCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAagt                                <  1:494055/150‑1 (MQ=255)
                                                                                                                    tGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAagtga                              >  1:1829618/1‑150 (MQ=255)
                                                                                                                    tGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAagtga                              >  1:9518/1‑150 (MQ=255)
                                                                                                                         cTTTTATATGGAGTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTTATGGCCTGCTGCGGTTGTTTGCTCTATCCGCTGAAGTTTAGTTTTATCGGTCCTGAAGTGAGTggg                         >  2:113209/1‑150 (MQ=255)
                                                                                                                           tttATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGt                       >  1:417055/1‑150 (MQ=255)
                                                                                                                               tatGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACg                   >  2:1440269/1‑150 (MQ=255)
                                                                                                                                atGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACgg                  >  1:174094/1‑150 (MQ=255)
                                                                                                                                  ggATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGGCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGa                <  1:479348/150‑1 (MQ=255)
                                                                                                                                    aTTTTCGCTGTTCGTTTGTAGCACGACATTCGAAAAGCGCCGACTTTTCAACCGGCTTATTATGGCAACGTTAATTGACTGCTGCGGTTTTTTGATCTATCAGCTGAAGTTTAGTTTTATCAGTCCTGAAGTGAGTGTGGTGACGGGTTg              >  1:1310248/1‑150 (MQ=255)
                                                                                                                                     tgttCGCTGTGCGTTTGAAGCACGACATTCGAACAGCGCCGACTTGCCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTCTCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATgg             <  1:296185/148‑1 (MQ=255)
                                                                                                                                      tttCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGc            <  2:417055/150‑1 (MQ=255)
                                                                                                                                      tttCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTGGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGc            <  1:279937/150‑1 (MQ=255)
                                                                                                                                       ggcGCTGTTCGAATGTCGTGCTACAAACGAACAGCGCCGCCTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCTCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGCCGGGATGGCt           >  2:1075390/3‑150 (MQ=37)
                                                                                                                                        gcGCTGTTCGAATGTCGTGCTACAAACGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTa          >  1:1655546/2‑150 (MQ=255)
                                                                                                                                        gcGCTGTTCGAATGTCGTGCTACAAACGAACAGCGCAGACTTGTCCGCCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCGTGAAGTGAGTGGGGTGACGGGATGGCTa          >  1:1843250/2‑150 (MQ=255)
                                                                                                                                         cGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCTGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTAt         <  2:852629/150‑1 (MQ=255)
                                                                                                                                           cTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTAttt       <  1:161196/150‑1 (MQ=255)
                                                                                                                                             gTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCTGCTTATTATTGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATACGTCCTGAAGTGAGTGGGGTGACGGGATGGATATTTTc     >  1:1697642/1‑150 (MQ=255)
                                                                                                                                             gTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTc     <  2:1465841/150‑1 (MQ=255)
                                                                                                                                              ttCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGTGTGACGGGATGGCTATTTTCg    <  1:1649551/150‑1 (MQ=255)
                                                                                                                                               tCGTTTGTAGCACGACATTCGTACAGCGCCGAGTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCAGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGc   <  2:1799797/150‑1 (MQ=255)
                                                                                                                                                cGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCa  <  2:287586/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCA  >  NC_000913/1480971‑1481264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: