Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,481,122 A→C 5.3% S72R (AGC→CGC) ‡ ynbD → putative phosphatase inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,481,1220AC 5.3% 274.1 / 4.3 94S72R (AGC→CGC) ‡ynbDputative phosphatase inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (43/46);  new base C (3/2);  total (46/48)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01

TAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGA  >  NC_000913/1480974‑1481270
                                                                                                                                                    |                                                                                                                                                    
tAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAg                                                                                                                                                     >  2:1093849/1‑150 (MQ=255)
    cAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACg                                                                                                                                                 >  1:1409149/1‑150 (MQ=255)
      gTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCACTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGAc                                                                                                                                               <  1:1525860/150‑1 (MQ=255)
         cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGATACGGCGAGCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACAtt                                                                                                                                            <  1:1103653/150‑1 (MQ=255)
         cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGCTCCATTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACAtt                                                                                                                                            >  1:96428/1‑150 (MQ=255)
         cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACAtt                                                                                                                                            >  1:852629/1‑150 (MQ=255)
          aCCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTCGACTATTGTTCCTTACTGGAGTCTGGATCGTTTATATTGATTTTCGCTGTTCGTATGTAGCACGACAtta                                                                                                                                           >  1:19560/1‑149 (MQ=255)
           ccGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCg                                                                                                                                          >  1:1141152/1‑150 (MQ=255)
           ccGCGGTTCAGGACCTTAACAGCCATGATATCACCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTAGCTGGACTATTGTTACTTACTGGAGTCTGGATCTTTTATATTGATTTTCGCTGTTCGTTTGTAGCACGACATTCg                                                                                                                                          >  2:1540920/1‑150 (MQ=255)
            cgcgCTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGACCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGa                                                                                                                                         <  2:268707/150‑1 (MQ=255)
              cgGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTTGGAATCGGCGATCCCTTTTCTTCCCTTGACTATTGTTCCTTACTTGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAAc                                                                                                                                       <  2:873342/150‑1 (MQ=255)
                  tCAGGACCTTAACAGCCATGATATCCCCAGTCTGGTCTTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCGGGACTATTGGTCCTTCCTGGAGTCTGGAGCTTTTATATGGATTTTCGCTGTTCGAATGTCGTGCTACAAACGAACAgcg                                                                                                                                   <  1:1409704/150‑1 (MQ=255)
                     ggACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCg                                                                                                                                <  2:58138/150‑1 (MQ=255)
                     ggACCTTAACAGCCATGAAATCCCCAGGCAGGTATTCGGGGGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCg                                                                                                                                <  1:1193864/150‑1 (MQ=255)
                      gACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGAATGTCGTGCTACAAACGAACAgcgaaaa                                                                                                                               <  1:749708/150‑5 (MQ=38)
                       aCCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGAc                                                                                                                              >  2:868675/1‑150 (MQ=255)
                        ccTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACt                                                                                                                             >  1:799047/1‑150 (MQ=255)
                        ccTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACt                                                                                                                             <  1:1657105/150‑1 (MQ=255)
                            aaCAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTc                                                                                                                         <  2:1786428/150‑1 (MQ=255)
                               aGCCATGATATCCCCAGTCAGGTTTTCGTTGGGGAAACTGCGATCCCTTTTCTTCCCTGGTCTATTGTTACTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCAc                                                                                                                      <  1:225006/150‑1 (MQ=255)
                                gCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTTTTGTTCCTTACGGGAGTCTGGTTCTTTTAGTTGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGCTCAACGCCGTCTTGTCGAcc                                                                                                                     >  2:1546683/1‑150 (MQ=255)
                                gCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCAcc                                                                                                                     >  1:1675920/1‑150 (MQ=255)
                                 ccATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCg                                                                                                                    >  1:287586/1‑150 (MQ=255)
                                  cATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCgg                                                                                                                   >  1:1090873/1‑150 (MQ=255)
                                    tGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCt                                                                                                                 <  1:1728800/150‑1 (MQ=255)
                                     gATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCtt                                                                                                                <  1:1091924/150‑1 (MQ=255)
                                       tatCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTAt                                                                                                              <  1:947012/150‑1 (MQ=255)
                                       tatCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTGCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTAt                                                                                                              <  2:1124916/150‑1 (MQ=255)
                                         tCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTc                                                                                                            >  1:1683629/1‑150 (MQ=255)
                                           cccAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTg                                                                                                          >  1:1465841/1‑150 (MQ=255)
                                            ccAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTgg                                                                                                         <  2:442353/150‑1 (MQ=255)
                                             cAGTCAGGTATTCGGTTGGGAAACGGCTATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGAGTTGTCCACCGGCTTATTCTGGc                                                                                                        <  1:524697/150‑1 (MQ=255)
                                             cAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGc                                                                                                        >  1:974145/1‑150 (MQ=255)
                                                  aGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACAGGCTTATTCTGGCAACgg                                                                                                   >  1:364001/1‑150 (MQ=255)
                                                        tCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGAATTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATgg                                                                                             <  2:880355/150‑1 (MQ=255)
                                                            ttGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctg                                                                                         <  1:1352089/150‑1 (MQ=255)
                                                            ttGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTAGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctg                                                                                         <  2:1625579/150‑1 (MQ=255)
                                                             tGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctgc                                                                                        >  1:996993/1‑150 (MQ=255)
                                                              gggAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctgct                                                                                       >  1:701572/1‑150 (MQ=255)
                                                               ggAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTGGTCCACCGGCGTATTCTGGCAACGGTAATGGCctgcag                                                                                      >  2:1649551/1‑148 (MQ=255)
                                                                gAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTTTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGGAATGGCCtgctgc                                                                                     >  2:498230/1‑150 (MQ=255)
                                                                  aaCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCgg                                                                                   <  1:1492411/150‑1 (MQ=255)
                                                                    cGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtt                                                                                 <  2:788225/150‑1 (MQ=255)
                                                                       cGATCCCTTTTCTTCCCTGGCCTATTGTTCCTTCCTGGAGTCTGGATCTTATATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                              <  2:1767488/150‑1 (MQ=255)
                                                                       cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGCGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGAAGCACGACATTCGAACAGCGCCGACTTGGCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTTCGGttttt                                                                              <  2:140779/150‑1 (MQ=255)
                                                                       cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                              <  2:1211895/150‑1 (MQ=255)
                                                                       cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                              >  2:161196/1‑150 (MQ=255)
                                                                       cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                              >  1:1111168/1‑150 (MQ=255)
                                                                        tataccTTTTCTTCCCTGTACTATTGTTCGTTAGTGGTGTCTGTATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACTACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATTGCCTGCTGCGGtttttt                                                                             <  1:1082754/146‑1 (MQ=255)
                                                                        gATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtttttt                                                                             <  1:1631753/150‑1 (MQ=255)
                                                                        gATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtttttt                                                                             <  1:930470/150‑1 (MQ=255)
                                                                        gATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTGCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtttttt                                                                             <  1:70589/150‑1 (MQ=255)
                                                                         aTCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTTGATCTTTTATATGGATTTTCGCTGATCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTg                                                                            <  1:293050/150‑1 (MQ=255)
                                                                              ttttCTTCCCTGGACTATTGTTACTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTa                                                                       <  2:96428/150‑1 (MQ=255)
                                                                                 tcttcCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATGCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATcc                                                                    <  1:1294167/150‑1 (MQ=255)
                                                                                    tcCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCAACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCt                                                                 >  1:1661529/1‑150 (MQ=255)
                                                                                     cccTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGGGGTGTTTTGCTCTATCCGCTg                                                                >  2:1874869/1‑150 (MQ=255)
                                                                                     cccTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTg                                                                <  2:546742/150‑1 (MQ=255)
                                                                                     cccTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTg                                                                >  1:1544954/1‑150 (MQ=255)
                                                                                         ggACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAagt                                                            <  1:1093849/150‑1 (MQ=255)
                                                                                                 gTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGtttt                                                    >  1:1617839/1‑150 (MQ=255)
                                                                                                    ccTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATc                                                 <  2:737146/150‑1 (MQ=255)
                                                                                                        aCTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTc                                             >  2:1552729/1‑150 (MQ=255)
                                                                                                         cTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTcc                                            <  2:682375/150‑1 (MQ=255)
                                                                                                         cTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTcc                                            <  2:1683629/150‑1 (MQ=255)
                                                                                                           ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                          >  1:989561/1‑150 (MQ=255)
                                                                                                           ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                          >  2:708355/1‑150 (MQ=255)
                                                                                                           ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                          >  2:708747/1‑150 (MQ=255)
                                                                                                           ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                          >  2:727470/1‑150 (MQ=255)
                                                                                                           ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                          >  1:1466370/1‑150 (MQ=255)
                                                                                                               tCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAagt                                      <  1:494055/150‑1 (MQ=255)
                                                                                                                 tGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAagtga                                    >  1:1829618/1‑150 (MQ=255)
                                                                                                                 tGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAagtga                                    >  1:9518/1‑150 (MQ=255)
                                                                                                                      cTTTTATATGGAGTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTTATGGCCTGCTGCGGTTGTTTGCTCTATCCGCTGAAGTTTAGTTTTATCGGTCCTGAAGTGAGTggg                               >  2:113209/1‑150 (MQ=255)
                                                                                                                        tttATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGt                             >  1:417055/1‑150 (MQ=255)
                                                                                                                            tatGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACg                         >  2:1440269/1‑150 (MQ=255)
                                                                                                                             atGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACgg                        >  1:174094/1‑150 (MQ=255)
                                                                                                                               ggATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGGCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGa                      <  1:479348/150‑1 (MQ=255)
                                                                                                                                 aTTTTCGCTGTTCGTTTGTAGCACGACATTCGAAAAGCGCCGACTTTTCAACCGGCTTATTATGGCAACGTTAATTGACTGCTGCGGTTTTTTGATCTATCAGCTGAAGTTTAGTTTTATCAGTCCTGAAGTGAGTGTGGTGACGGGTTg                    >  1:1310248/1‑150 (MQ=255)
                                                                                                                                  tgttCGCTGTGCGTTTGAAGCACGACATTCGAACAGCGCCGACTTGCCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTCTCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATgg                   <  1:296185/148‑1 (MQ=255)
                                                                                                                                   tttCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGc                  <  2:417055/150‑1 (MQ=255)
                                                                                                                                   tttCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTGGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGc                  <  1:279937/150‑1 (MQ=255)
                                                                                                                                    ggcGCTGTTCGAATGTCGTGCTACAAACGAACAGCGCCGCCTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCTCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGCCGGGATGGCt                 >  2:1075390/3‑150 (MQ=37)
                                                                                                                                     gcGCTGTTCGAATGTCGTGCTACAAACGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTa                >  1:1655546/2‑150 (MQ=255)
                                                                                                                                     gcGCTGTTCGAATGTCGTGCTACAAACGAACAGCGCAGACTTGTCCGCCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCGTGAAGTGAGTGGGGTGACGGGATGGCTa                >  1:1843250/2‑150 (MQ=255)
                                                                                                                                      cGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCTGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTAt               <  2:852629/150‑1 (MQ=255)
                                                                                                                                        cTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTAttt             <  1:161196/150‑1 (MQ=255)
                                                                                                                                          gTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCTGCTTATTATTGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATACGTCCTGAAGTGAGTGGGGTGACGGGATGGATATTTTc           >  1:1697642/1‑150 (MQ=255)
                                                                                                                                          gTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTc           <  2:1465841/150‑1 (MQ=255)
                                                                                                                                           ttCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGTGTGACGGGATGGCTATTTTCg          <  1:1649551/150‑1 (MQ=255)
                                                                                                                                            tCGTTTGTAGCACGACATTCGTACAGCGCCGAGTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCAGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGc         <  2:1799797/150‑1 (MQ=255)
                                                                                                                                             cGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCa        <  2:287586/150‑1 (MQ=255)
                                                                                                                                                   tAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGa  >  1:532914/1‑150 (MQ=255)
                                                                                                                                                   tAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGa  >  1:1579900/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGA  >  NC_000913/1480974‑1481270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: