Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,481,125 A→G 5.3% T73A (ACG→GCG) ‡ ynbD → putative phosphatase inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,481,1250AG 5.3% 271.7 / 5.0 95T73A (ACG→GCG) ‡ynbDputative phosphatase inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (43/47);  new base G (3/2);  total (46/49)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.54e-01

CAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAAC  >  NC_000913/1480978‑1481272
                                                                                                                                                   |                                                                                                                                                   
cAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACg                                                                                                                                                   >  1:1409149/1‑150 (MQ=255)
  gTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCACTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGAc                                                                                                                                                 <  1:1525860/150‑1 (MQ=255)
     cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGATACGGCGAGCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACAtt                                                                                                                                              <  1:1103653/150‑1 (MQ=255)
     cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGCTCCATTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACAtt                                                                                                                                              >  1:96428/1‑150 (MQ=255)
     cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACAtt                                                                                                                                              >  1:852629/1‑150 (MQ=255)
      aCCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTCGACTATTGTTCCTTACTGGAGTCTGGATCGTTTATATTGATTTTCGCTGTTCGTATGTAGCACGACAtta                                                                                                                                             >  1:19560/1‑149 (MQ=255)
       ccGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCg                                                                                                                                            >  1:1141152/1‑150 (MQ=255)
       ccGCGGTTCAGGACCTTAACAGCCATGATATCACCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTAGCTGGACTATTGTTACTTACTGGAGTCTGGATCTTTTATATTGATTTTCGCTGTTCGTTTGTAGCACGACATTCg                                                                                                                                            >  2:1540920/1‑150 (MQ=255)
        cgcgCTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGACCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGa                                                                                                                                           <  2:268707/150‑1 (MQ=255)
          cgGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTTGGAATCGGCGATCCCTTTTCTTCCCTTGACTATTGTTCCTTACTTGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAAc                                                                                                                                         <  2:873342/150‑1 (MQ=255)
              tCAGGACCTTAACAGCCATGATATCCCCAGTCTGGTCTTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCGGGACTATTGGTCCTTCCTGGAGTCTGGAGCTTTTATATGGATTTTCGCTGTTCGAATGTCGTGCTACAAACGAACAgcg                                                                                                                                     <  1:1409704/150‑1 (MQ=255)
                 ggACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCg                                                                                                                                  <  2:58138/150‑1 (MQ=255)
                 ggACCTTAACAGCCATGAAATCCCCAGGCAGGTATTCGGGGGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCg                                                                                                                                  <  1:1193864/150‑1 (MQ=255)
                  gACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGAATGTCGTGCTACAAACGAACAgcgaaaa                                                                                                                                 <  1:749708/150‑5 (MQ=38)
                   aCCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGAc                                                                                                                                >  2:868675/1‑150 (MQ=255)
                    ccTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACt                                                                                                                               >  1:799047/1‑150 (MQ=255)
                    ccTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACt                                                                                                                               <  1:1657105/150‑1 (MQ=255)
                        aaCAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTc                                                                                                                           <  2:1786428/150‑1 (MQ=255)
                           aGCCATGATATCCCCAGTCAGGTTTTCGTTGGGGAAACTGCGATCCCTTTTCTTCCCTGGTCTATTGTTACTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCAc                                                                                                                        <  1:225006/150‑1 (MQ=255)
                            gCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTTTTGTTCCTTACGGGAGTCTGGTTCTTTTAGTTGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGCTCAACGCCGTCTTGTCGAcc                                                                                                                       >  2:1546683/1‑150 (MQ=255)
                            gCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCAcc                                                                                                                       >  1:1675920/1‑150 (MQ=255)
                             ccATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCg                                                                                                                      >  1:287586/1‑150 (MQ=255)
                              cATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCgg                                                                                                                     >  1:1090873/1‑150 (MQ=255)
                                tGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCt                                                                                                                   <  1:1728800/150‑1 (MQ=255)
                                 gATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCtt                                                                                                                  <  1:1091924/150‑1 (MQ=255)
                                   tatCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTAt                                                                                                                <  1:947012/150‑1 (MQ=255)
                                   tatCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTGCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTAt                                                                                                                <  2:1124916/150‑1 (MQ=255)
                                     tCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTc                                                                                                              >  1:1683629/1‑150 (MQ=255)
                                       cccAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTg                                                                                                            >  1:1465841/1‑150 (MQ=255)
                                        ccAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTgg                                                                                                           <  2:442353/150‑1 (MQ=255)
                                         cAGTCAGGTATTCGGTTGGGAAACGGCTATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGAGTTGTCCACCGGCTTATTCTGGc                                                                                                          <  1:524697/150‑1 (MQ=255)
                                         cAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGc                                                                                                          >  1:974145/1‑150 (MQ=255)
                                              aGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACAGGCTTATTCTGGCAACgg                                                                                                     >  1:364001/1‑150 (MQ=255)
                                                    tCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGAATTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATgg                                                                                               <  2:880355/150‑1 (MQ=255)
                                                        ttGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctg                                                                                           <  1:1352089/150‑1 (MQ=255)
                                                        ttGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTAGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctg                                                                                           <  2:1625579/150‑1 (MQ=255)
                                                         tGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctgc                                                                                          >  1:996993/1‑150 (MQ=255)
                                                          gggAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCctgct                                                                                         >  1:701572/1‑150 (MQ=255)
                                                           ggAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTGGTCCACCGGCGTATTCTGGCAACGGTAATGGCctgcag                                                                                        >  2:1649551/1‑148 (MQ=255)
                                                            gAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTTTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGGAATGGCCtgctgc                                                                                       >  2:498230/1‑150 (MQ=255)
                                                              aaCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCgg                                                                                     <  1:1492411/150‑1 (MQ=255)
                                                                cGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtt                                                                                   <  2:788225/150‑1 (MQ=255)
                                                                   cGATCCCTTTTCTTCCCTGGCCTATTGTTCCTTCCTGGAGTCTGGATCTTATATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                                <  2:1767488/150‑1 (MQ=255)
                                                                   cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGCGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGAAGCACGACATTCGAACAGCGCCGACTTGGCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTTCGGttttt                                                                                <  2:140779/150‑1 (MQ=255)
                                                                   cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                                >  1:1111168/1‑150 (MQ=255)
                                                                   cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                                <  2:1211895/150‑1 (MQ=255)
                                                                   cGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGttttt                                                                                >  2:161196/1‑150 (MQ=255)
                                                                    tataccTTTTCTTCCCTGTACTATTGTTCGTTAGTGGTGTCTGTATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACTACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATTGCCTGCTGCGGtttttt                                                                               <  1:1082754/146‑1 (MQ=255)
                                                                    gATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtttttt                                                                               <  1:1631753/150‑1 (MQ=255)
                                                                    gATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtttttt                                                                               <  1:930470/150‑1 (MQ=255)
                                                                    gATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTGCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGtttttt                                                                               <  1:70589/150‑1 (MQ=255)
                                                                     aTCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTTGATCTTTTATATGGATTTTCGCTGATCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTg                                                                              <  1:293050/150‑1 (MQ=255)
                                                                          ttttCTTCCCTGGACTATTGTTACTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTa                                                                         <  2:96428/150‑1 (MQ=255)
                                                                             tcttcCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATGCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATcc                                                                      <  1:1294167/150‑1 (MQ=255)
                                                                                tcCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCAACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCt                                                                   >  1:1661529/1‑150 (MQ=255)
                                                                                 cccTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGGGGTGTTTTGCTCTATCCGCTg                                                                  >  2:1874869/1‑150 (MQ=255)
                                                                                 cccTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTg                                                                  >  1:1544954/1‑150 (MQ=255)
                                                                                 cccTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTg                                                                  <  2:546742/150‑1 (MQ=255)
                                                                                     ggACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAagt                                                              <  1:1093849/150‑1 (MQ=255)
                                                                                             gTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGtttt                                                      >  1:1617839/1‑150 (MQ=255)
                                                                                                ccTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATc                                                   <  2:737146/150‑1 (MQ=255)
                                                                                                    aCTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTc                                               >  2:1552729/1‑150 (MQ=255)
                                                                                                     cTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTcc                                              <  2:682375/150‑1 (MQ=255)
                                                                                                     cTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTcc                                              <  2:1683629/150‑1 (MQ=255)
                                                                                                       ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                            >  2:708355/1‑150 (MQ=255)
                                                                                                       ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                            >  2:708747/1‑150 (MQ=255)
                                                                                                       ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                            >  2:727470/1‑150 (MQ=255)
                                                                                                       ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                            >  1:1466370/1‑150 (MQ=255)
                                                                                                       ggAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTg                                            >  1:989561/1‑150 (MQ=255)
                                                                                                           tCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAagt                                        <  1:494055/150‑1 (MQ=255)
                                                                                                             tGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAagtga                                      >  1:9518/1‑150 (MQ=255)
                                                                                                             tGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAagtga                                      >  1:1829618/1‑150 (MQ=255)
                                                                                                                  cTTTTATATGGAGTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTTATGGCCTGCTGCGGTTGTTTGCTCTATCCGCTGAAGTTTAGTTTTATCGGTCCTGAAGTGAGTggg                                 >  2:113209/1‑150 (MQ=255)
                                                                                                                    tttATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGt                               >  1:417055/1‑150 (MQ=255)
                                                                                                                        tatGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACg                           >  2:1440269/1‑150 (MQ=255)
                                                                                                                         atGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACgg                          >  1:174094/1‑150 (MQ=255)
                                                                                                                           ggATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGGCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGa                        <  1:479348/150‑1 (MQ=255)
                                                                                                                             aTTTTCGCTGTTCGTTTGTAGCACGACATTCGAAAAGCGCCGACTTTTCAACCGGCTTATTATGGCAACGTTAATTGACTGCTGCGGTTTTTTGATCTATCAGCTGAAGTTTAGTTTTATCAGTCCTGAAGTGAGTGTGGTGACGGGTTg                      >  1:1310248/1‑150 (MQ=255)
                                                                                                                              tgttCGCTGTGCGTTTGAAGCACGACATTCGAACAGCGCCGACTTGCCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTCTCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATgg                     <  1:296185/148‑1 (MQ=255)
                                                                                                                               tttCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGc                    <  2:417055/150‑1 (MQ=255)
                                                                                                                               tttCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTGGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGc                    <  1:279937/150‑1 (MQ=255)
                                                                                                                                ggcGCTGTTCGAATGTCGTGCTACAAACGAACAGCGCCGCCTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCTCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGCCGGGATGGCt                   >  2:1075390/3‑150 (MQ=37)
                                                                                                                                 gcGCTGTTCGAATGTCGTGCTACAAACGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTa                  >  1:1655546/2‑150 (MQ=255)
                                                                                                                                 gcGCTGTTCGAATGTCGTGCTACAAACGAACAGCGCAGACTTGTCCGCCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCGTGAAGTGAGTGGGGTGACGGGATGGCTa                  >  1:1843250/2‑150 (MQ=255)
                                                                                                                                  cGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCTGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTAt                 <  2:852629/150‑1 (MQ=255)
                                                                                                                                    cTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTAttt               <  1:161196/150‑1 (MQ=255)
                                                                                                                                      gTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCTGCTTATTATTGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATACGTCCTGAAGTGAGTGGGGTGACGGGATGGATATTTTc             >  1:1697642/1‑150 (MQ=255)
                                                                                                                                      gTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTc             <  2:1465841/150‑1 (MQ=255)
                                                                                                                                       ttCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGTGTGACGGGATGGCTATTTTCg            <  1:1649551/150‑1 (MQ=255)
                                                                                                                                        tCGTTTGTAGCACGACATTCGTACAGCGCCGAGTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCAGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGc           <  2:1799797/150‑1 (MQ=255)
                                                                                                                                         cGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCa          <  2:287586/150‑1 (MQ=255)
                                                                                                                                               tAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGa    >  1:1579900/1‑150 (MQ=255)
                                                                                                                                               tAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGa    >  1:532914/1‑150 (MQ=255)
                                                                                                                                                 gCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGAGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAAc  >  1:174516/1‑150 (MQ=255)
                                                                                                                                                 gCACGACATTCGAACAGCGCCGACTTGCCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTGAGTTTTATCCGTCCTGAAGTGAGGGGGGTGACGGGATGGCTATTTTAGCAACTTGAAc  <  2:99162/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTAATGGCCTGCTGCGGTTTTTTGCTCTATCCGCTGAAGTTTAGTTTTATCCGTCCTGAAGTGAGTGGGGTGACGGGATGGCTATTTTCGCAACTTGAAC  >  NC_000913/1480978‑1481272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: