Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,536,879 T→A 5.4% E144V (GAG→GTG)  narW ← nitrate reductase 2 (NRZ), delta subunit (assembly subunit)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,536,8790TA 5.4% 423.5 / 8.9 140E144V (GAG→GTG) narWnitrate reductase 2 (NRZ), delta subunit (assembly subunit)
Reads supporting (aligned to +/- strand):  ref base T (62/70);  new base A (4/4);  total (66/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01

Only 100 of 142 total aligned reads displayed.

ACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGG  >  NC_000913/1536730‑1537025
                                                                                                                                                     |                                                                                                                                                   
aCTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCt                                                                                                                                                     <  2:1305618/150‑1 (MQ=255)
      ccTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgtt                                                                                                                                               <  1:1056346/150‑1 (MQ=255)
      ccTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgtt                                                                                                                                               >  2:1552737/1‑150 (MQ=255)
        tGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgttgt                                                                                                                                             <  1:1806710/150‑1 (MQ=255)
         gTTCCGCTTCCCACACTTCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCgttgtt                                                                                                                                            <  1:1212955/150‑1 (MQ=255)
          ttCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGttt                                                                                                                                           <  1:1266496/150‑1 (MQ=255)
          ttCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGttt                                                                                                                                           <  1:619143/150‑1 (MQ=255)
          ttCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGAGGCACCGTGGTACGCGTTGttt                                                                                                                                           <  2:1796288/150‑1 (MQ=255)
           tCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGAGGCACCGTGGTACGCGTTGtttg                                                                                                                                          <  1:863982/150‑2 (MQ=255)
             ctctTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACGTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTGGTTTTaa                                                                                                                                        >  1:778186/1‑150 (MQ=255)
              tctTCCCACACTGCATCAAGCGCCTGGCGGGAGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAg                                                                                                                                       <  1:450654/150‑1 (MQ=255)
                ttCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGAGGCACCGTGGTACGCGTTGtttgatgct                                                                                                                                     <  2:753387/150‑7 (MQ=38)
                  cccACACTGCATCAAGCGCCTGGCGGGTGTCCTCGCGCTCTTCGCTGTTGACTTTTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGCGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGAc                                                                                                                                   >  2:1371763/1‑150 (MQ=255)
                    cacaCTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCg                                                                                                                                 <  2:891678/150‑1 (MQ=255)
                    cacaCTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCg                                                                                                                                 >  2:12216/1‑150 (MQ=255)
                        cTGCATCAAGCGCCTGGCGGGTGTCAACGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCaa                                                                                                                             >  2:365814/1‑150 (MQ=255)
                          gCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGc                                                                                                                           >  1:1115342/1‑150 (MQ=255)
                          gCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGc                                                                                                                           <  1:1627835/150‑1 (MQ=255)
                            aTCAAGCGCCTGGCGGGTGTCAGCGCGCTCTACGCTGTTTAATTGTTTTGGGACACTGTCACGTGAAAGGGAGCGTCCCGCCAGTGGCAGCAGAGCAGCAACCAACCCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAg                                                                                                                         <  1:81595/150‑1 (MQ=255)
                               aaGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGc                                                                                                                      >  2:511094/1‑150 (MQ=255)
                                   gcCTTGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGAAACTGTCACTTGAAAGGGAGCGTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAgg                                                                                                                  <  1:122579/150‑1 (MQ=255)
                                    ccTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGa                                                                                                                 >  1:939700/1‑150 (MQ=255)
                                       ggCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCg                                                                                                              <  2:98635/150‑1 (MQ=255)
                                       ggCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCg                                                                                                              <  1:1208831/150‑1 (MQ=255)
                                       ggCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCg                                                                                                              >  1:1637593/1‑150 (MQ=255)
                                        gCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACTCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCgg                                                                                                             <  2:1143121/150‑1 (MQ=255)
                                        gCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCCCGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCgg                                                                                                             <  1:719362/150‑1 (MQ=255)
                                          gggTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGgcg                                                                                                           <  1:155556/150‑1 (MQ=255)
                                          gggTGTCACCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGgcg                                                                                                           <  1:962853/150‑1 (MQ=255)
                                           ggTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGgcgc                                                                                                          <  1:714462/150‑1 (MQ=255)
                                               tCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACa                                                                                                      >  1:740742/1‑150 (MQ=255)
                                                    gcgcTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACTGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAg                                                                                                 <  2:1262432/150‑1 (MQ=255)
                                                     cgcTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGc                                                                                                <  1:1339590/150‑1 (MQ=255)
                                                         ctTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATc                                                                                            >  2:628494/1‑150 (MQ=255)
                                                              cTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCcttct                                                                                       <  1:1348333/150‑1 (MQ=255)
                                                                     cTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCt                                                                                >  1:1516763/1‑150 (MQ=255)
                                                                       tGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGa                                                                              <  2:1401199/150‑1 (MQ=255)
                                                                       tGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGa                                                                              >  2:382685/1‑150 (MQ=255)
                                                                        ttcaagtgACAGTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGAt                                                                             >  1:1005198/6‑150 (MQ=255)
                                                                        gTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGAt                                                                             >  2:1077019/1‑150 (MQ=255)
                                                                         ttttGTGACAGTGTCACTTGAATGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAAGAACGCGTACCTCGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGTTCGGCGCAACTTTGAGCAATCCTTCTTTCGCCTgttt                                                                            <  1:245008/150‑4 (MQ=255)
                                                                            tgtgACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTc                                                                         <  2:1388397/150‑1 (MQ=255)
                                                                                    tggcACTTGAACGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACACCGCGTCCCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTa                                                                 <  2:778186/147‑1 (MQ=255)
                                                                                     gTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTAc                                                                >  2:1646573/1‑150 (MQ=255)
                                                                                      tCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACg                                                               <  2:1516763/150‑1 (MQ=255)
                                                                                      tCACTTGAAAGGGAGCATCCCGCCAGTAGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACg                                                               <  1:918215/150‑1 (MQ=255)
                                                                                       cACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGc                                                              <  1:1408948/150‑1 (MQ=255)
                                                                                        aCTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCt                                                             >  2:1306077/1‑150 (MQ=255)
                                                                                        aCTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCt                                                             >  2:806260/1‑150 (MQ=255)
                                                                                          ttGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTa                                                           <  1:74283/150‑1 (MQ=255)
                                                                                          ttGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTCTTTTAAGCGACCGCCAAGCAGGGCGAGGCTCGGCGCCACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTTCGCGTa                                                           >  1:1686079/1‑150 (MQ=255)
                                                                                            gAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTaaa                                                         >  2:1226398/1‑150 (MQ=255)
                                                                                            gAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTaaa                                                         <  2:1781957/150‑1 (MQ=255)
                                                                                             aaaGGGAGCTTCCCGCCAGTTGCAGCAGAGTATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAAt                                                        <  1:1767108/150‑1 (MQ=255)
                                                                                             aaaGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAAt                                                        <  1:1504241/150‑1 (MQ=255)
                                                                                             aaaGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAAt                                                        >  2:1486566/1‑150 (MQ=255)
                                                                                             aaaGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCGTAAAt                                                        >  2:535882/1‑150 (MQ=255)
                                                                                                gggAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACt                                                     >  2:1529578/1‑150 (MQ=255)
                                                                                                    gCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAg                                                 >  1:28488/1‑150 (MQ=255)
                                                                                                     cTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGCGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGa                                                >  1:548947/1‑150 (MQ=255)
                                                                                                      ttCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGTTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGAt                                               <  1:385890/150‑1 (MQ=255)
                                                                                                      ttCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGCTCGTCAGGCAGTACGCTTAAATACTCCAGAt                                               >  1:54549/1‑150 (MQ=255)
                                                                                                      ttCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGAt                                               <  1:592665/150‑1 (MQ=255)
                                                                                                      ttCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGAt                                               <  1:94029/150‑1 (MQ=255)
                                                                                                       tCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATa                                              <  2:1264973/150‑1 (MQ=255)
                                                                                                             cAGTGGCAGCAGAGCATCAAACAACGCGTACCACGGGGCCTCGCGTTGTTTTAAGCGACCGCCACGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCgg                                        <  2:1146172/150‑1 (MQ=255)
                                                                                                               gTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTa                                      <  1:173627/150‑1 (MQ=255)
                                                                                                                ttGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGTGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAg                                     <  1:540233/150‑1 (MQ=255)
                                                                                                                ttGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAg                                     <  2:1005198/150‑1 (MQ=255)
                                                                                                                ttGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAg                                     <  1:806260/150‑1 (MQ=255)
                                                                                                                 tGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCGTTCGCCTGCTCGTCAGGCAGTCCGCTTAAATACGCCAGATACAGCGGTAGa                                   >  2:182464/1‑150 (MQ=255)
                                                                                                                  gcagcaGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGCTCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGAt                                  >  1:1221721/1‑150 (MQ=255)
                                                                                                                   cagcagAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACAGTGAGCACTCCTTGTTTCGCCTGATCGGCAGGCAGTACGCTTAAATACTCCAGATAGAGCGGTAGATa                                 >  2:1174623/1‑150 (MQ=255)
                                                                                                                     gcagAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCCAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAAt                               >  2:1417930/1‑150 (MQ=255)
                                                                                                                       agagCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCg                             >  1:507935/1‑150 (MQ=255)
                                                                                                                       agagCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCg                             <  1:1415084/150‑1 (MQ=255)
                                                                                                                        gagCATCTAACAACGCGTACCTCGGTGGCTCGCGTTGTTTTAAGCGTCCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTAGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCgg                            <  1:365427/150‑1 (MQ=255)
                                                                                                                        gagCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCgg                            <  2:1223/150‑1 (MQ=255)
                                                                                                                         agCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGTATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCggg                           <  2:1672879/150‑1 (MQ=255)
                                                                                                                           cATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCATTCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCa                         >  1:415950/1‑150 (MQ=255)
                                                                                                                           cATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCa                         >  2:1813731/1‑150 (MQ=255)
                                                                                                                            agcAAACAACGCGTACACCGGTGCCTCTCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAg                        <  1:1146147/148‑1 (MQ=255)
                                                                                                                            aTCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGCTACAGCGGTAGATAATCGGGCAg                        >  2:505054/1‑150 (MQ=255)
                                                                                                                            aTCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAg                        >  2:1279588/1‑150 (MQ=255)
                                                                                                                             taaaaCAACGCGAGGCACCGTGGTACGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGCGCAATCCTTCTTTCGCCTGATCGTAAGGCAGGGCGCTTAAATACTCCTGATTCAGCGGTAGATAATCGGGCCGt                       >  2:145290/3‑150 (MQ=255)
                                                                                                                              cAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGtt                      >  2:678025/1‑150 (MQ=255)
                                                                                                                              cAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGtt                      >  1:803209/1‑150 (MQ=255)
                                                                                                                               aaaCAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTc                     <  2:740742/150‑1 (MQ=255)
                                                                                                                                 acaacGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTccc                   <  1:1022823/150‑1 (MQ=255)
                                                                                                                                    acGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGAc                <  1:152731/150‑1 (MQ=255)
                                                                                                                                     cgcgTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTATTTCGCCTGATCTTCAGGCAGTTCGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGGTACCGCCa               >  2:1174843/1‑150 (MQ=255)
                                                                                                                                       cgTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAAt             <  1:148780/150‑1 (MQ=255)
                                                                                                                                           ccACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAg         >  1:1761143/1‑150 (MQ=255)
                                                                                                                                            cACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGCCAATCCAGc        >  2:635079/1‑150 (MQ=255)
                                                                                                                                            cACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGc        <  1:769038/150‑1 (MQ=255)
                                                                                                                                            cACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGc        <  1:365814/150‑1 (MQ=255)
                                                                                                                                             aCGGTGCCTCGAGTTGTTTTAACCGTCCGCCAAGCAGGGCGAGGATCGGCGCAACATGGAGAAAACCTTCGTTCGCCGGATCGTCTGGCAGGACGCTGAAAGACTGCAGCTTCAGCGGTAGCATAACGGGCAGTTCCGGACAGTCCAGcg       >  2:263438/1‑149 (MQ=255)
                                                                                                                                               ggTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCTGGGCGAGGAGCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCTGTTCCCGACACTCCAGCTGc     >  1:73075/1‑150 (MQ=255)
                                                                                                                                                gTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCa    <  1:511094/150‑1 (MQ=255)
                                                                                                                                                  gCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAgg  >  1:1394655/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ACTTCACCTGTTCCTCTTCCCACACTGCATCAAGCGCCTGGCGGGTGTCATCGCGCTCTTCGCTGTTTACTTGTTTTGTGACACTGTCACTTGAAAGGGAGCTTCCCGCCAGTTGCAGCAGAGCATCAAACAACGCGTACCACGGTGCCTCGCGTTGTTTTAAGCGACCGCCAAGCAGGGCGAGGATCGGCGCAACATTGAGCAATCCTTCTTTCGCCTGATCGTCAGGCAGTACGCTTAAATACTCCAGATACAGCGGTAGATAATCGGGCAGTTCCCGACAATCCAGCTGCAGG  >  NC_000913/1536730‑1537025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: