Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,555,784 G→C 5.0% intergenic (‑115/+42) maeA ← / ← sra malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme/stationary‑phase‑induced ribosome‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,555,7840GC 5.0% 351.6 / 9.4 119intergenic (‑115/+42)maeA/sramalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme/stationary‑phase‑induced ribosome‑associated protein
Reads supporting (aligned to +/- strand):  ref base G (50/63);  new base C (2/4);  total (52/67)
Fisher's exact test for biased strand distribution p-value = 6.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01

Only 100 of 123 total aligned reads displayed.

TAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAA  >  NC_000913/1555635‑1555932
                                                                                                                                                     |                                                                                                                                                    
taAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTTAATATCAATCACTGGGGGGCTTTTATCGTCTTTGCTTTAACGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATAGCTCAg                                                                                                                                                      >  2:1436758/1‑150 (MQ=255)
 aaaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAgg                                                                                                                                                     <  1:42768/150‑1 (MQ=255)
  aaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGc                                                                                                                                                    <  1:1682259/150‑1 (MQ=255)
        aCGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGgt                                                                                                                                              <  2:771935/150‑1 (MQ=255)
         cGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGgtg                                                                                                                                             <  1:1672323/150‑1 (MQ=255)
           cTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGtt                                                                                                                                           <  2:438080/150‑1 (MQ=255)
             gTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTCTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTa                                                                                                                                         >  1:1296259/1‑150 (MQ=255)
              tttttttGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTaa                                                                                                                                        <  2:638705/150‑1 (MQ=255)
               ttttttGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAATTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGGTGtttttg                                                                                                                                       <  2:1089769/150‑4 (MQ=255)
                 ttttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTAATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAACCACCATTGCGCAGGCAATGGTGTTTAATCg                                                                                                                                     <  2:1475433/150‑1 (MQ=255)
                  tttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCACTGGGGGGCTTTTATCGTCATTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGt                                                                                                                                    >  2:931670/1‑150 (MQ=255)
                   ttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGGTGtttttgtttt                                                                                                                                   <  2:966437/150‑8 (MQ=255)
                    tgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAATCAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCa                                                                                                                                  >  1:1209585/1‑150 (MQ=255)
                      tttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCTTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCAtt                                                                                                                                >  1:1015507/1‑150 (MQ=255)
                      tttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCAtt                                                                                                                                >  1:1560491/1‑150 (MQ=255)
                       ttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCGTCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTg                                                                                                                               <  2:853584/150‑1 (MQ=255)
                       ttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCTCAGGCAATGGTGTTTAATCGTCAttt                                                                                                                               >  1:445321/1‑149 (MQ=255)
                       ttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTg                                                                                                                               <  1:1306014/150‑1 (MQ=255)
                            ggtCCAAGTCACTCACTCTTTTTTGAATATCCCTCCCTGGGGGGCTTATATCGTCTGTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCTATGGTGTTTAATCGTCATTGAGGAc                                                                                                                          <  2:896214/148‑1 (MQ=255)
                            gTTCCATGTCACTCACTCTCTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCAATGCGCAGGCAATGGTGTTTAATCGTCATTGAGGAc                                                                                                                          >  2:1171954/1‑150 (MQ=255)
                             ttCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACt                                                                                                                         <  1:1782006/150‑1 (MQ=255)
                                cATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGAt                                                                                                                      <  1:1168408/150‑1 (MQ=255)
                                cATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGAt                                                                                                                      <  1:447638/150‑1 (MQ=255)
                                 aTGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATg                                                                                                                     <  1:647709/150‑1 (MQ=255)
                                  tGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATgg                                                                                                                    <  1:1467270/150‑1 (MQ=255)
                                  tGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATgg                                                                                                                    <  1:918171/150‑1 (MQ=255)
                                  tGTCACTCACTCTTTTTTGAATATCCATCCCTGGGAGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATgg                                                                                                                    <  2:935369/150‑1 (MQ=255)
                                   gTCTCTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGTGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGt                                                                                                                   <  2:1271632/150‑1 (MQ=255)
                                   gTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGt                                                                                                                   <  2:1278739/150‑1 (MQ=255)
                                      actcactcTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATg                                                                                                               <  2:1034659/150‑1 (MQ=255)
                                         cactcTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGTCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGAGTTTAATCGTCATTGAGGACTGATGGTTATGat                                                                                                             >  1:112502/1‑149 (MQ=255)
                                         cactcTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGaa                                                                                                             <  2:970471/150‑1 (MQ=255)
                                         cactcTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGaa                                                                                                             >  1:1770376/1‑150 (MQ=255)
                                          actcTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAAt                                                                                                            <  2:22257/150‑1 (MQ=255)
                                           ctctTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAAtt                                                                                                           <  2:1707934/150‑1 (MQ=255)
                                             ggtttttGCCTATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGGAAGGGCTTAGGACTCAAGTATAAAGCTGATTAAAAAAAAAACTCCATTGCGCAGGCCAGGGGGTTTAATCGTCATTGAGGTCTGATGGTTATGATTTAc                                                                                                         >  1:96069/3‑150 (MQ=255)
                                             ctTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTAc                                                                                                         >  2:648101/1‑150 (MQ=255)
                                                ttttGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACttt                                                                                                      <  1:1699885/150‑1 (MQ=255)
                                                   tGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCa                                                                                                   <  1:1094580/150‑1 (MQ=255)
                                                    taaTATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAg                                                                                                  <  2:543571/149‑1 (MQ=255)
                                                        atccatccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCggg                                                                                              <  2:1649709/150‑1 (MQ=255)
                                                          ccatccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGc                                                                                            <  1:766493/150‑1 (MQ=255)
                                                            atccCTGGGGTGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGt                                                                                          <  1:1867602/150‑1 (MQ=255)
                                                             tccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGtt                                                                                         >  1:13150/1‑150 (MQ=255)
                                                             tccCTGGGGGGCTTTTATAGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGtt                                                                                         >  1:1441936/1‑150 (MQ=255)
                                                              cccTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACATCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGttt                                                                                        <  1:1628881/150‑1 (MQ=255)
                                                              cccTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGttt                                                                                        <  2:1295318/150‑1 (MQ=255)
                                                                  ggggggCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTg                                                                                    <  2:195409/150‑1 (MQ=255)
                                                                     gggCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCg                                                                                 <  2:29800/150‑1 (MQ=255)
                                                                       gCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGCGAATGGAGTTTAATCGTAATTTAGGAATGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGACGGt                                                                               >  2:289996/1‑150 (MQ=255)
                                                                        cTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGtt                                                                              >  2:1409256/1‑150 (MQ=255)
                                                                           ttATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTggg                                                                           <  1:577442/150‑1 (MQ=255)
                                                                           ttATCGTCATTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTggg                                                                           <  1:1650992/150‑1 (MQ=255)
                                                                            tATCGTCTTTGCTTTACCGCCAGGGCGTCGGGCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGt                                                                          <  1:187092/150‑1 (MQ=255)
                                                                               cGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTTATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAt                                                                       >  2:456739/1‑150 (MQ=255)
                                                                               cGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAt                                                                       <  2:1377882/150‑1 (MQ=255)
                                                                                gTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCTTTGAGGTCTGATGGGTATGAATTAGTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAtt                                                                      >  2:1734873/1‑150 (MQ=255)
                                                                                gTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAtt                                                                      <  1:454748/150‑1 (MQ=255)
                                                                                 tCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAttt                                                                     <  2:513948/150‑1 (MQ=255)
                                                                                     tGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTtacta                                                                 <  2:789605/150‑1 (MQ=255)
                                                                                       cTTTACCGCCAGGGCGTCGGCCTCATGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCAGTGAGGACTGATGGTTATGTATTACTTTTCAGCGGGGCGTTTTCGGGCGGTTGGGTTATTTACTTCg                                                               >  1:252963/1‑150 (MQ=255)
                                                                                       cTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATAGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACg                                                               >  1:1821665/1‑150 (MQ=255)
                                                                                        tttACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGc                                                              >  2:557741/1‑150 (MQ=255)
                                                                                         ttACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCt                                                             >  2:1345096/1‑150 (MQ=255)
                                                                                          tACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACGGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGACGGTTGGGTTAGTTACTACGCTg                                                            >  2:215003/1‑150 (MQ=255)
                                                                                           aCCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTgg                                                           <  2:1347283/150‑1 (MQ=255)
                                                                                             cGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAGTTACTACGCTGGAt                                                         >  2:187054/1‑150 (MQ=255)
                                                                                              gCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGAtt                                                        >  1:748028/1‑150 (MQ=255)
                                                                                              gCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGAtt                                                        >  2:1193668/1‑150 (MQ=255)
                                                                                                 aGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGt                                                     >  2:418282/1‑150 (MQ=255)
                                                                                                  gggCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTc                                                    >  1:590668/1‑150 (MQ=255)
                                                                                                  gggCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACGGATGGTTATGAATTACTTTTCAGCGTGGCGGTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTc                                                    <  2:1638242/150‑1 (MQ=255)
                                                                                                   ggCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGGAATGGTGTTTAATCGTCATTGAGGCCTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCa                                                   <  1:972696/150‑1 (MQ=255)
                                                                                                      gTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCt                                                >  1:742771/1‑150 (MQ=255)
                                                                                                         ggCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCg                                             >  2:685370/1‑150 (MQ=255)
                                                                                                          gCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCgg                                            <  2:1296259/150‑1 (MQ=255)
                                                                                                          gCCTCAAGTATAAAGAAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTATAAACGACATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGATTGGGTTATTTACTACGCTGGATTTGTCACATTCgg                                            >  1:1398265/1‑150 (MQ=255)
                                                                                                           ccTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGt                                           >  2:1602078/1‑150 (MQ=255)
                                                                                                              cAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAAc                                        >  1:1461280/1‑150 (MQ=255)
                                                                                                              cAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAAc                                        >  1:721329/1‑150 (MQ=255)
                                                                                                              cAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAAc                                        <  2:1723126/150‑1 (MQ=255)
                                                                                                                aGTATAAATCAGATAAAAACAAAAACACCATTTCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGGTATTTACTACGCTGGATTTGTCACCTTCGGTAACTa                                      <  2:1442984/150‑1 (MQ=255)
                                                                                                                    taAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTAtttt                                  <  2:1015507/150‑1 (MQ=255)
                                                                                                                      aaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTGTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTgc                                >  2:811634/1‑150 (MQ=255)
                                                                                                                      aaGCAGATAAAAACAAAAACACCATTGAGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATTGTTATGAATTACTTTTCAGCGGTGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTgc                                <  2:309682/150‑1 (MQ=255)
                                                                                                                        gCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGTTTATGAATAACTTTTCAGCGGGGCGTTTTCTGCCTGTTGGTTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTgcgc                              <  1:167081/150‑1 (MQ=255)
                                                                                                                        gCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTgcgc                              <  1:1455543/150‑1 (MQ=255)
                                                                                                                           gATAAAAACAAAAACACCATTGCGCAGGCAATTGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTgg                           <  2:1553687/150‑1 (MQ=255)
                                                                                                                               aaaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAg                       >  2:1115960/1‑150 (MQ=255)
                                                                                                                                aaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCGGGTTGGTTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCTCTGGTTAGa                      >  2:1432188/1‑150 (MQ=255)
                                                                                                                                  acaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGaaa                    <  2:1770376/150‑1 (MQ=255)
                                                                                                                                    aaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAtt                  <  2:748028/150‑1 (MQ=255)
                                                                                                                                      aaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAtttt                <  2:1582717/150‑1 (MQ=255)
                                                                                                                                      aaacaCCATTGCCTGCGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAtttt                >  2:319729/1‑150 (MQ=255)
                                                                                                                                        acacCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTAt              <  1:741978/150‑1 (MQ=255)
                                                                                                                                            cATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtc          >  2:873201/1‑150 (MQ=255)
                                                                                                                                            cATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtc          >  2:830233/1‑150 (MQ=255)
                                                                                                                                             aTTGCGCAGGCAAGGGTGTTTAATCGTCATTGAGGACTGATGGTTATGATTTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAAGTAATTTGCGCTGGTTAGAAATTTTATGgtca         >  2:863886/1‑149 (MQ=255)
                                                                                                                                                gcgcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtccagt      <  1:1844976/150‑1 (MQ=255)
                                                                                                                                                    aGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCaa  >  1:180358/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCCAA  >  NC_000913/1555635‑1555932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: