Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,596,501 T→A 5.3% pseudogene (1487/3861 nt) yneO ← pseudogene, AidA homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,596,5010TA 5.3% 306.4 / 4.7 105pseudogene (1487/3861 nt)yneOpseudogene, AidA homolog
Reads supporting (aligned to +/- strand):  ref base T (49/50);  new base A (4/2);  total (53/52)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 108 total aligned reads displayed.

TATCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCT  >  NC_000913/1596352‑1596650
                                                                                                                                                     |                                                                                                                                                     
tATCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCAt                                                                                                                                                       >  2:118500/1‑150 (MQ=255)
    aCGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGATATATTAACTATCCCCTTACCGGAGGCATTtcc                                                                                                                                                   >  2:1051122/1‑150 (MQ=255)
    aCGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcc                                                                                                                                                   >  2:1773124/1‑150 (MQ=255)
     cgcgCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcct                                                                                                                                                  >  1:881830/1‑150 (MQ=255)
       cgcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcctcc                                                                                                                                                >  1:1127916/1‑150 (MQ=255)
       cgcTTTAACAATACCTCCGGTGGTAATATTCAGGTCACCCGTCCCTAATACACCGACCTGTAGCAATTGTGAGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcctcc                                                                                                                                                <  2:297322/150‑1 (MQ=255)
        gcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCg                                                                                                                                               <  2:1403732/150‑1 (MQ=255)
         gtttAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGAAATGCCTCCgg                                                                                                                                              <  2:164149/149‑2 (MQ=255)
             aaCTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAGCTATCCCCTTACCGGAGGCATTTCCTCCGATaaa                                                                                                                                          >  1:52749/1‑150 (MQ=255)
              aCTATACCTCCGGTGGTAATATTCAGATCACCCGTGCCTAATACACCGACCTGTAGCAATTCTGCGTTAGTAGATGATGTCTTTAAAATCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGAAATGCCTCCGGTaagg                                                                                                                                         <  1:104082/150‑3 (MQ=255)
               cTATACCTCCGGTGGTAATATTCTGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGTAGGCATTTCCTCCGATAAATc                                                                                                                                        <  1:920037/150‑1 (MQ=255)
                tataCCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATcc                                                                                                                                       >  2:1861607/1‑150 (MQ=255)
                 ataCCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCa                                                                                                                                      >  2:1605777/1‑150 (MQ=255)
                      tCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGcc                                                                                                                                 <  1:1554569/150‑1 (MQ=255)
                        cGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCg                                                                                                                               <  2:418344/150‑1 (MQ=255)
                        cGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCg                                                                                                                               <  2:461529/150‑1 (MQ=255)
                        cGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCg                                                                                                                               <  2:803733/150‑1 (MQ=255)
                          gtggtAATATTCAGTTCACCCGTGCCTAATCCCCCGACCGGTAGCAATTGTGCGGTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCAGATAAATCCATAGCCCGtt                                                                                                                             >  2:941868/1‑150 (MQ=255)
                           tggtAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTg                                                                                                                            <  1:1080288/150‑1 (MQ=255)
                             gtAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGaa                                                                                                                          <  1:1103983/150‑1 (MQ=255)
                               aaTATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAAtt                                                                                                                        >  2:236010/1‑150 (MQ=255)
                               aaTATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAAtt                                                                                                                        <  1:104829/150‑1 (MQ=255)
                                   ttCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTTTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGAGTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAgg                                                                                                                    <  1:607548/150‑1 (MQ=255)
                                   ttCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGCCTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAgg                                                                                                                    >  1:895785/1‑150 (MQ=255)
                                     cAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGt                                                                                                                  >  2:108837/1‑150 (MQ=255)
                                      aGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATTATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTg                                                                                                                 <  1:1486280/150‑1 (MQ=255)
                                       gTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGAATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTgg                                                                                                                >  2:1773003/1‑150 (MQ=255)
                                         tCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCa                                                                                                              >  1:825715/1‑150 (MQ=255)
                                         tCAACCGTGCCTAATACACCGAACTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCa                                                                                                              <  2:1089468/150‑1 (MQ=255)
                                              cGTGCCTTATACACCGACCTTTAGCAATTGTACGTTATTAGATTATGTCTTTAAATTCCATATACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCAtt                                                                                                         <  2:140545/150‑1 (MQ=255)
                                              cGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCAtt                                                                                                         <  1:1596646/150‑1 (MQ=255)
                                                tGCCTAATACACCGACCTGTAGCAATTGGGCGTTAGTAGAAGATGTCTTTAAAGTCCAGAGACTGTCCGTAGAAATATTAACTAACCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTAc                                                                                                       <  2:1848769/150‑1 (MQ=255)
                                                 gCCTAATACACCGACCTGTAGCAATTCTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGATGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACt                                                                                                      <  2:1168598/150‑1 (MQ=255)
                                                  ccTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTg                                                                                                     <  1:1557723/150‑1 (MQ=255)
                                                   cTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGa                                                                                                    <  1:1605777/150‑1 (MQ=255)
                                                   cTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGa                                                                                                    <  1:439398/150‑1 (MQ=255)
                                                   cTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGa                                                                                                    >  2:1269049/1‑150 (MQ=255)
                                                    tAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGAc                                                                                                   <  2:1374037/150‑1 (MQ=255)
                                                     aaTACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACa                                                                                                  <  1:1684163/150‑1 (MQ=255)
                                                     aaTACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACa                                                                                                  >  2:824230/1‑150 (MQ=255)
                                                      aTACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCGTTAAATTCCAGAGACTGTCCGTTGAAATATTACCTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTGGAATTATGGGTGGCCCAATTACTGACAt                                                                                                 >  2:1146228/1‑150 (MQ=255)
                                                         cacCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTACGGGTGGCACCATTACTGACATTTa                                                                                              >  1:413873/1‑150 (MQ=255)
                                                         cacCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTa                                                                                              >  1:561242/1‑150 (MQ=255)
                                                         cacCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATAAACTATCCCCTTACCGGAGGAATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTa                                                                                              <  2:1206198/150‑1 (MQ=255)
                                                           ccGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACg                                                                                            <  1:546603/150‑1 (MQ=255)
                                                              aCCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCACCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATa                                                                                         >  2:1484564/1‑150 (MQ=255)
                                                                cTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATAcc                                                                                       >  1:592735/1‑150 (MQ=255)
                                                                cTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTAACCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGGTGAATTCAGGGTGGCACCATTACTGACATTTACGATAcc                                                                                       <  2:1127916/150‑1 (MQ=255)
                                                                 tGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCAATTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCg                                                                                      >  1:777814/1‑150 (MQ=255)
                                                                    aGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTa                                                                                   >  2:1183258/1‑150 (MQ=255)
                                                                    aGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTa                                                                                   <  1:382555/150‑1 (MQ=255)
                                                                            tgCGTTAGTAGATGATGTCTTTAACTTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAAtt                                                                           >  1:1323701/1‑150 (MQ=255)
                                                                               gTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGATGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTAcc                                                                        <  1:280932/150‑1 (MQ=255)
                                                                                ttAGTTGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCg                                                                       <  1:1461735/150‑1 (MQ=255)
                                                                                  agtagATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGAc                                                                     >  1:1830161/1‑150 (MQ=255)
                                                                                     agATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACAtt                                                                  <  1:118500/150‑1 (MQ=255)
                                                                                     agATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACAtt                                                                  >  1:1254979/1‑150 (MQ=255)
                                                                                     agATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACAtt                                                                  <  1:1783984/150‑1 (MQ=255)
                                                                                          atgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATGGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATAc                                                             >  1:1372296/1‑150 (MQ=255)
                                                                                          atgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATAc                                                             >  1:609606/1‑150 (MQ=255)
                                                                                              cTTTATATTCCAGAGACTGTCCGTTGAAATATTTACTATCCCCTTTCCGGAGGCATTTCCTCCGATAAATCAATAGCCCGTTTAATTAAGTGTGGCACCATTACTGACATTTACTATACCGCTACCAAAATTACCGTCATTGATACCcac                                                         <  1:1125824/150‑1 (MQ=255)
                                                                                              cTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAATGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTTCCGACATTGATACCcac                                                         <  1:236010/150‑1 (MQ=255)
                                                                                              cTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATacacac                                                         >  2:1801392/1‑150 (MQ=255)
                                                                                               tttAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCcaca                                                        >  1:679962/1‑150 (MQ=255)
                                                                                                  aaaTTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGATGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTa                                                     <  2:546619/150‑1 (MQ=255)
                                                                                                  aaaTTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTa                                                     >  1:269003/1‑150 (MQ=255)
                                                                                                   aaTTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTAt                                                    >  1:404431/1‑150 (MQ=255)
                                                                                                      tCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGa                                                 >  1:852573/1‑150 (MQ=255)
                                                                                                       ccAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGaa                                                >  1:436884/1‑150 (MQ=255)
                                                                                                        cAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGCCATTGATACCCAGAGTATCGAAc                                               >  1:473793/1‑150 (MQ=255)
                                                                                                        cAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAAc                                               >  1:1591950/1‑150 (MQ=255)
                                                                                                         agagACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACg                                              >  1:200437/1‑150 (MQ=255)
                                                                                                           agaCTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgat                                            <  1:35605/150‑1 (MQ=255)
                                                                                                               tGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGatgatg                                        <  1:1178741/150‑1 (MQ=255)
                                                                                                                gTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAAAGATGATGt                                       >  2:90095/1‑150 (MQ=255)
                                                                                                                   cGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGc                                    <  1:1795234/150‑1 (MQ=255)
                                                                                                                      tGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGcatc                                 <  1:1773124/150‑1 (MQ=255)
                                                                                                                       gAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGcatcc                                <  2:1525889/150‑1 (MQ=255)
                                                                                                                       gAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGcatcc                                >  2:1837082/1‑150 (MQ=255)
                                                                                                                          atatTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGcatccatc                             >  1:474100/1‑150 (MQ=255)
                                                                                                                            atTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCCCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAg                           >  1:1524141/1‑150 (MQ=255)
                                                                                                                            atTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCAGAGTATCGAACGATGATGTCGCTTCCATCAg                           >  1:1224397/1‑150 (MQ=255)
                                                                                                                                aCTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTc                       <  1:1183258/150‑1 (MQ=255)
                                                                                                                                   atggatttATCGGAGGAAATGCCTCCGGTAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAgt                    >  2:406978/7‑150 (MQ=255)
                                                                                                                                    tCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCTCCATTACTGAGATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCTTCAGGTTCAgtg                   >  1:739468/1‑150 (MQ=255)
                                                                                                                                    tCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAgtg                   <  1:1598844/150‑1 (MQ=255)
                                                                                                                                    tCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAgtg                   <  2:1254979/150‑1 (MQ=255)
                                                                                                                                          tatcGGAGGAAATGCCTCCGGTAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGACGa             >  1:601539/4‑150 (MQ=255)
                                                                                                                                          tatcGGAGGAAATGCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCGTCCAAAATTACCGACATTGATACCCACAGTAGCGAACGAGGATGTCGCATCCATCAGGGTACGTGTGCCGa             >  1:1336483/4‑150 (MQ=255)
                                                                                                                                          tACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGGTCAGTGTGCCGa             <  2:1384212/150‑1 (MQ=255)
                                                                                                                                             cGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATAc          >  1:945157/1‑150 (MQ=255)
                                                                                                                                              ggAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATAcc         >  2:803273/1‑150 (MQ=255)
                                                                                                                                               gAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCg        <  2:206064/150‑1 (MQ=255)
                                                                                                                                                 ggCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgct      <  2:399339/150‑1 (MQ=255)
                                                                                                                                                 ggCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgct      <  1:1126997/150‑1 (MQ=255)
                                                                                                                                                   cATTTCCTCCGATAAATCCATAGCCCGTTGAATTACGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgcttg    <  2:413873/150‑1 (MQ=255)
                                                                                                                                                   cATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgcttg    >  1:124977/1‑150 (MQ=255)
                                                                                                                                                   cATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgcttg    >  2:1755730/1‑150 (MQ=255)
                                                                                                                                                     tttCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgcttgct  <  2:1035074/150‑1 (MQ=255)
                                                                                                                                                     tttCCTCCGATAAATCCATAGCACGTTGAATTAAGGGTGGCACCATTAATGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgcttgct  <  1:290409/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TATCACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCT  >  NC_000913/1596352‑1596650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: