Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,596,503 T→G 5.1% pseudogene (1485/3861 nt) yneO ← pseudogene, AidA homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,596,5030TG 5.1% 325.2 / 5.7 99pseudogene (1485/3861 nt)yneOpseudogene, AidA homolog
Reads supporting (aligned to +/- strand):  ref base T (45/49);  new base G (3/2);  total (48/51)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 108 total aligned reads displayed.

CACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCTT  >  NC_000913/1596355‑1596651
                                                                                                                                                    |                                                                                                                                                    
 aCGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGATATATTAACTATCCCCTTACCGGAGGCATTtcc                                                                                                                                                    >  2:1051122/1‑150 (MQ=255)
 aCGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcc                                                                                                                                                    >  2:1773124/1‑150 (MQ=255)
  cgcgCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcct                                                                                                                                                   >  1:881830/1‑150 (MQ=255)
    cgcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcctcc                                                                                                                                                 >  1:1127916/1‑150 (MQ=255)
    cgcTTTAACAATACCTCCGGTGGTAATATTCAGGTCACCCGTCCCTAATACACCGACCTGTAGCAATTGTGAGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTtcctcc                                                                                                                                                 <  2:297322/150‑1 (MQ=255)
     gcTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCg                                                                                                                                                <  2:1403732/150‑1 (MQ=255)
      gtttAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGAAATGCCTCCgg                                                                                                                                               <  2:164149/149‑2 (MQ=255)
          aaCTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAGCTATCCCCTTACCGGAGGCATTTCCTCCGATaaa                                                                                                                                           >  1:52749/1‑150 (MQ=255)
           aCTATACCTCCGGTGGTAATATTCAGATCACCCGTGCCTAATACACCGACCTGTAGCAATTCTGCGTTAGTAGATGATGTCTTTAAAATCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGAAATGCCTCCGGTaagg                                                                                                                                          <  1:104082/150‑3 (MQ=255)
            cTATACCTCCGGTGGTAATATTCTGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGTAGGCATTTCCTCCGATAAATc                                                                                                                                         <  1:920037/150‑1 (MQ=255)
             tataCCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATcc                                                                                                                                        >  2:1861607/1‑150 (MQ=255)
              ataCCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCa                                                                                                                                       >  2:1605777/1‑150 (MQ=255)
                   tCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGcc                                                                                                                                  <  1:1554569/150‑1 (MQ=255)
                     cGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCg                                                                                                                                <  2:418344/150‑1 (MQ=255)
                     cGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCg                                                                                                                                <  2:461529/150‑1 (MQ=255)
                     cGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCg                                                                                                                                <  2:803733/150‑1 (MQ=255)
                       gtggtAATATTCAGTTCACCCGTGCCTAATCCCCCGACCGGTAGCAATTGTGCGGTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCAGATAAATCCATAGCCCGtt                                                                                                                              >  2:941868/1‑150 (MQ=255)
                        tggtAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTg                                                                                                                             <  1:1080288/150‑1 (MQ=255)
                          gtAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGaa                                                                                                                           <  1:1103983/150‑1 (MQ=255)
                            aaTATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAAtt                                                                                                                         >  2:236010/1‑150 (MQ=255)
                            aaTATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAAtt                                                                                                                         <  1:104829/150‑1 (MQ=255)
                                ttCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTTTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGAGTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAgg                                                                                                                     <  1:607548/150‑1 (MQ=255)
                                ttCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGCCTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAgg                                                                                                                     >  1:895785/1‑150 (MQ=255)
                                  cAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGt                                                                                                                   >  2:108837/1‑150 (MQ=255)
                                   aGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATTATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTg                                                                                                                  <  1:1486280/150‑1 (MQ=255)
                                    gTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGAATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTgg                                                                                                                 >  2:1773003/1‑150 (MQ=255)
                                      tCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCa                                                                                                               >  1:825715/1‑150 (MQ=255)
                                      tCAACCGTGCCTAATACACCGAACTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCa                                                                                                               <  2:1089468/150‑1 (MQ=255)
                                           cGTGCCTTATACACCGACCTTTAGCAATTGTACGTTATTAGATTATGTCTTTAAATTCCATATACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCAtt                                                                                                          <  2:140545/150‑1 (MQ=255)
                                           cGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCAtt                                                                                                          <  1:1596646/150‑1 (MQ=255)
                                             tGCCTAATACACCGACCTGTAGCAATTGGGCGTTAGTAGAAGATGTCTTTAAAGTCCAGAGACTGTCCGTAGAAATATTAACTAACCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTAc                                                                                                        <  2:1848769/150‑1 (MQ=255)
                                              gCCTAATACACCGACCTGTAGCAATTCTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGATGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACt                                                                                                       <  2:1168598/150‑1 (MQ=255)
                                               ccTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTg                                                                                                      <  1:1557723/150‑1 (MQ=255)
                                                cTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGa                                                                                                     >  2:1269049/1‑150 (MQ=255)
                                                cTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGa                                                                                                     <  1:1605777/150‑1 (MQ=255)
                                                cTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGa                                                                                                     <  1:439398/150‑1 (MQ=255)
                                                 tAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGAc                                                                                                    <  2:1374037/150‑1 (MQ=255)
                                                  aaTACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACa                                                                                                   <  1:1684163/150‑1 (MQ=255)
                                                  aaTACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACa                                                                                                   >  2:824230/1‑150 (MQ=255)
                                                   aTACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCGTTAAATTCCAGAGACTGTCCGTTGAAATATTACCTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTGGAATTATGGGTGGCCCAATTACTGACAt                                                                                                  >  2:1146228/1‑150 (MQ=255)
                                                      cacCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTACGGGTGGCACCATTACTGACATTTa                                                                                               >  1:413873/1‑150 (MQ=255)
                                                      cacCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTa                                                                                               >  1:561242/1‑150 (MQ=255)
                                                      cacCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATAAACTATCCCCTTACCGGAGGAATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTa                                                                                               <  2:1206198/150‑1 (MQ=255)
                                                        ccGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACg                                                                                             <  1:546603/150‑1 (MQ=255)
                                                             cTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATAcc                                                                                        >  1:592735/1‑150 (MQ=255)
                                                             cTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTAACCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGGTGAATTCAGGGTGGCACCATTACTGACATTTACGATAcc                                                                                        <  2:1127916/150‑1 (MQ=255)
                                                              tGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCAATTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCg                                                                                       >  1:777814/1‑150 (MQ=255)
                                                                 aGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTa                                                                                    <  1:382555/150‑1 (MQ=255)
                                                                 aGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTa                                                                                    >  2:1183258/1‑150 (MQ=255)
                                                                         tgCGTTAGTAGATGATGTCTTTAACTTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAAtt                                                                            >  1:1323701/1‑150 (MQ=255)
                                                                            gTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGATGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTAcc                                                                         <  1:280932/150‑1 (MQ=255)
                                                                             ttAGTTGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCg                                                                        <  1:1461735/150‑1 (MQ=255)
                                                                               agtagATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGAc                                                                      >  1:1830161/1‑150 (MQ=255)
                                                                                gtagATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCAGTGGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCGACCAAAAATACCGCCa                                                                     >  2:1464784/1‑150 (MQ=255)
                                                                                  agATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACAtt                                                                   <  1:118500/150‑1 (MQ=255)
                                                                                  agATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACAtt                                                                   >  1:1254979/1‑150 (MQ=255)
                                                                                  agATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACAtt                                                                   <  1:1783984/150‑1 (MQ=255)
                                                                                       atgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATGGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATAc                                                              >  1:1372296/1‑150 (MQ=255)
                                                                                       atgTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATAc                                                              >  1:609606/1‑150 (MQ=255)
                                                                                           cTTTATATTCCAGAGACTGTCCGTTGAAATATTTACTATCCCCTTTCCGGAGGCATTTCCTCCGATAAATCAATAGCCCGTTTAATTAAGTGTGGCACCATTACTGACATTTACTATACCGCTACCAAAATTACCGTCATTGATACCcac                                                          <  1:1125824/150‑1 (MQ=255)
                                                                                           cTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAATGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTTCCGACATTGATACCcac                                                          <  1:236010/150‑1 (MQ=255)
                                                                                           cTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATacacac                                                          >  2:1801392/1‑150 (MQ=255)
                                                                                            tttAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCcaca                                                         >  1:679962/1‑150 (MQ=255)
                                                                                               aaaTTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGATGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTa                                                      <  2:546619/150‑1 (MQ=255)
                                                                                               aaaTTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTa                                                      >  1:269003/1‑150 (MQ=255)
                                                                                                aaTTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTAt                                                     >  1:404431/1‑150 (MQ=255)
                                                                                                   tCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGa                                                  >  1:852573/1‑150 (MQ=255)
                                                                                                    ccAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGaa                                                 >  1:436884/1‑150 (MQ=255)
                                                                                                     cAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGCCATTGATACCCAGAGTATCGAAc                                                >  1:473793/1‑150 (MQ=255)
                                                                                                     cAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAAc                                                >  1:1591950/1‑150 (MQ=255)
                                                                                                      agagACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACg                                               >  1:200437/1‑150 (MQ=255)
                                                                                                        agaCTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACgat                                             <  1:35605/150‑1 (MQ=255)
                                                                                                            tGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGatgatg                                         <  1:1178741/150‑1 (MQ=255)
                                                                                                             gTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAAAGATGATGt                                        >  2:90095/1‑150 (MQ=255)
                                                                                                                cGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGc                                     <  1:1795234/150‑1 (MQ=255)
                                                                                                                   tGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGcatc                                  <  1:1773124/150‑1 (MQ=255)
                                                                                                                    gAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGcatcc                                 <  2:1525889/150‑1 (MQ=255)
                                                                                                                    gAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGcatcc                                 >  2:1837082/1‑150 (MQ=255)
                                                                                                                       atatTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGcatccatc                              >  1:474100/1‑150 (MQ=255)
                                                                                                                         atTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCCCCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAg                            >  1:1524141/1‑150 (MQ=255)
                                                                                                                         atTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCAGAGTATCGAACGATGATGTCGCTTCCATCAg                            >  1:1224397/1‑150 (MQ=255)
                                                                                                                             aCTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTc                        <  1:1183258/150‑1 (MQ=255)
                                                                                                                                atggatttATCGGAGGAAATGCCTCCGGTAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAgt                     >  2:406978/7‑150 (MQ=255)
                                                                                                                                 tCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCTCCATTACTGAGATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCTTCAGGTTCAgtg                    >  1:739468/1‑150 (MQ=255)
                                                                                                                                 tCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAgtg                    <  2:1254979/150‑1 (MQ=255)
                                                                                                                                 tCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAgtg                    <  1:1598844/150‑1 (MQ=255)
                                                                                                                                       tatcGGAGGAAATGCCTCCGGTAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGACGa              >  1:601539/4‑150 (MQ=255)
                                                                                                                                       tatcGGAGGAAATGCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCGTCCAAAATTACCGACATTGATACCCACAGTAGCGAACGAGGATGTCGCATCCATCAGGGTACGTGTGCCGa              >  1:1336483/4‑150 (MQ=255)
                                                                                                                                       tACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGGTCAGTGTGCCGa              <  2:1384212/150‑1 (MQ=255)
                                                                                                                                          cGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATAc           >  1:945157/1‑150 (MQ=255)
                                                                                                                                           ggAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATAcc          >  2:803273/1‑150 (MQ=255)
                                                                                                                                            gAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCg         <  2:206064/150‑1 (MQ=255)
                                                                                                                                              ggCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgct       <  2:399339/150‑1 (MQ=255)
                                                                                                                                              ggCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgct       <  1:1126997/150‑1 (MQ=255)
                                                                                                                                                cATTTCCTCCGATAAATCCATAGCCCGTTGAATTACGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgcttg     <  2:413873/150‑1 (MQ=255)
                                                                                                                                                cATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgcttg     >  1:124977/1‑150 (MQ=255)
                                                                                                                                                cATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgcttg     >  2:1755730/1‑150 (MQ=255)
                                                                                                                                                  tttCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgcttgct   <  2:1035074/150‑1 (MQ=255)
                                                                                                                                                  tttCCTCCGATAAATCCATAGCACGTTGAATTAAGGGTGGCACCATTAATGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCgcttgct   <  1:290409/150‑1 (MQ=255)
                                                                                                                                                   ttCCTCCGATAAATCAATAGCCCGTGGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGAGCCGATACCgcttgctt  <  2:280838/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CACGCGCTTTAACTATACCTCCGGTGGTAATATTCAGTTCACCCGTGCCTAATACACCGACCTGTAGCAATTGTGCGTTAGTAGATGATGTCTTTAAATTCCAGAGACTGTCCGTTGAAATATTAACTATCCCCTTACCGGAGGCATTTCCTCCGATAAATCCATAGCCCGTTGAATTAAGGGTGGCACCATTACTGACATTTACGATACCGCTACCAAAATTACCGACATTGATACCCACAGTATCGAACGATGATGTCGCATCCATCAGGTTCAGTGTGCCGATACCGCTTGCTT  >  NC_000913/1596355‑1596651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: