Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,660,194 A→C 10.2% K709T (AAG→ACG)  ynfE → putative selenate reductase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,660,1940AC10.2% 252.4 / 14.2 99K709T (AAG→ACG) ynfEputative selenate reductase, periplasmic
Reads supporting (aligned to +/- strand):  ref base A (45/43);  new base C (3/8);  total (48/51)
Fisher's exact test for biased strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTT  >  NC_000913/1660047‑1660339
                                                                                                                                                   |                                                                                                                                                 
aaGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAgg                                                                                                                                                 >  1:1635325/1‑150 (MQ=255)
 aGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGc                                                                                                                                                >  2:1476218/1‑150 (MQ=255)
     gAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGctgcc                                                                                                                                            >  2:1707781/1‑150 (MQ=255)
     gAAGTGATAAGCCCATTGCCAGTTTACGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGctgcc                                                                                                                                            <  1:1298096/150‑1 (MQ=255)
          gaaaatcccATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCACTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCAATTACAAATCCCGTACTCACTCCACCTACGGGAATATTGAACTCCTGAAGGCTGCCTGCCg                                                                                                                                       >  1:73737/7‑150 (MQ=255)
             aaGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCa                                                                                                                                    >  1:644124/1‑150 (MQ=255)
              aGCCCATTGCCAGTTTATGCCTAAACCTTAGAAGGCAGGAACTCCCCTGAACGTAGAACCTTCCCCCTGCAACTGTTTGGGTTCCATTACAAAACCCGTACTAACACGCCCAACGCCAATATTGAACTCCTGAAGGATGCCTGCCGTCac                                                                                                                                   >  1:1848087/1‑149 (MQ=255)
               gCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCagg                                                                                                                                  <  1:745073/150‑1 (MQ=255)
                cccATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGACGGCAGGCAGCCTTCagga                                                                                                                                 <  1:1070638/150‑1 (MQ=255)
                 ccATTGCCCGTTTATGCCTCAACCTTTGAAGGCGGGAACTCCCTTTAACTTATATACTTCCCACTGCAACTGTTTGGTTTACATTACAAATCACGTACTCACTCGACCTACGGCAATATTGCTCTCCTGACGGCAGGCAGCCTTCaggag                                                                                                                                <  2:343255/150‑1 (MQ=255)
                 ccATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGACGGCAGGCAGCCTTCaggag                                                                                                                                <  1:409824/150‑1 (MQ=255)
                  cATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCaggagg                                                                                                                               >  1:23696/1‑150 (MQ=255)
                     tGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGgtgt                                                                                                                            >  2:691438/1‑150 (MQ=255)
                     tGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGACCTCCTGACGGCAGGCAGCCTTCaggagatca                                                                                                                            <  1:426823/150‑5 (MQ=255)
                     tGCCAGTATATGCCTCAACCTTTGACGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTGTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGgtgt                                                                                                                            <  1:959172/150‑1 (MQ=255)
                       ccAGTTTATGCCTCATCCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTgg                                                                                                                          <  2:269727/150‑1 (MQ=255)
                       ccAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGACGGCAGGCAGCCTTCaggagatcaat                                                                                                                          <  1:1061650/150‑7 (MQ=255)
                        cAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGCTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGa                                                                                                                         <  1:1410483/150‑1 (MQ=255)
                           tttATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCa                                                                                                                      <  1:1517406/150‑1 (MQ=255)
                           tttATGCCTCAACCGTTGACGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCCACTCGTTGCGTTCCATTACAAATCCCCTCCTCACACGCCCTACGGCAATCATGATCTCCTGACGGCTGCCTGCCGTCAGGAGGTGTGGATCa                                                                                                                      <  2:120735/150‑1 (MQ=255)
                             tATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAAc                                                                                                                    <  1:664489/150‑1 (MQ=255)
                              aTGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTATCCATTACAAATCCCGTACTCACTCGACCTACCGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGAGGATAAAcc                                                                                                                   >  1:109998/1‑150 (MQ=255)
                                 ccacAACCATTGAAGGCTGGAACTCCCCTGAACGTACAACCTTCCCACTGCATCTGTTTGGTTTCAATTACAAATCCCGTACTCACTCGACCTACGCCAATATTGATCTCCTGAAGGATGACTGCCGACAGGAGGTGTGGATCAACCCta                                                                                                                >  2:812366/4‑150 (MQ=255)
                                 ccTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCta                                                                                                                >  1:246721/1‑150 (MQ=255)
                                 ccTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCAATCGTCCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGGGTGGATCACCCCta                                                                                                                >  1:920376/1‑150 (MQ=255)
                                   tCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCtata                                                                                                              <  1:993475/150‑1 (MQ=255)
                                    ccaCCTTTGTAGGCTGGAACTCCCGTGAACGTAGAACCTTCCCACTGCTACTGTTTGGTTTCCTTTACAAATCCCGTACTCTCTCGACATACGTCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAg                                                                                                             <  1:360948/148‑1 (MQ=255)
                                    cAACCTTTGACGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTTCTCACTCGACCTACGGCAATATTGATCTCCTCACGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAg                                                                                                             <  2:387092/150‑1 (MQ=255)
                                       ccTTTGAAGGCTGGAACTCCCCTGAACGTCGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATg                                                                                                          <  2:516937/150‑1 (MQ=255)
                                       ccTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATg                                                                                                          >  1:634516/1‑150 (MQ=255)
                                       ccTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGGGTGGATCAACCCTATAGATg                                                                                                          >  2:843399/1‑150 (MQ=255)
                                           tGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCa                                                                                                      <  2:1408047/150‑1 (MQ=255)
                                            gAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAg                                                                                                     >  2:448236/1‑150 (MQ=255)
                                                    gAACTCCCCTGAACTTAGATCCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTgg                                                                                             <  2:595295/150‑1 (MQ=255)
                                                    gAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTgg                                                                                             <  1:990408/150‑1 (MQ=255)
                                                    gAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTgg                                                                                             >  1:1385078/1‑150 (MQ=255)
                                                    gAACACCACTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTgg                                                                                             <  1:271207/150‑1 (MQ=255)
                                                     aaCTCCCCTGAACGTAGAACCTACCCACTGCAACTGTTTGGTTTCCATTACAAATCCCTTACTCACTCGACATACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTggg                                                                                            <  2:1126370/150‑1 (MQ=255)
                                                      aCTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAACGTGGGa                                                                                           >  1:689425/1‑150 (MQ=255)
                                                        tCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGAtt                                                                                         <  2:1812584/150‑1 (MQ=255)
                                                         ccccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTg                                                                                        >  2:850973/1‑150 (MQ=255)
                                                         ccccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTg                                                                                        <  1:502975/150‑1 (MQ=255)
                                                         accatGAACGTAGAACCTTCCCACTGCAACTGATTGGGTTCCATTACAACTCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGGCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTg                                                                                        <  1:277372/146‑1 (MQ=255)
                                                          cccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGc                                                                                       <  2:129880/150‑1 (MQ=255)
                                                           ccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGcc                                                                                      >  2:1456414/1‑150 (MQ=255)
                                                           ccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGcc                                                                                      <  1:691438/150‑1 (MQ=255)
                                                                aCGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACgg                                                                                 <  1:1867988/150‑1 (MQ=255)
                                                                 cGTAGAACCTTCCCCCTGCAACTGTTTGGTTTCCATTCCAACTCCCGAACTCACTCGACCTACGGCAATATTGAGCTCCTGAAGGCTGCATGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACggt                                                                                <  2:45490/150‑2 (MQ=255)
                                                                 cGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGc                                                                                >  2:1457144/1‑150 (MQ=255)
                                                                    cgAACCTGTCCAGTGCATCTGTTTGCTTTCCATTACAAATCCCGTACTCAATCTCCCTTTGGCAAAATTGTTGTCGTGTAGGCGGCCAGCCGTCAGGAGGTGGGGTTCAAGCGTAAATCTGCGCAGAAAGGTGGGATTGCCACCGGTGat                                                                             <  2:1778852/149‑1 (MQ=255)
                                                                    aGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGat                                                                             >  2:252265/1‑150 (MQ=255)
                                                                    aGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGACTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGat                                                                             >  1:105228/1‑150 (MQ=255)
                                                                     gAACCTTCCCACAGCAACTGTTTGGTTACAATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGata                                                                            <  2:1170886/150‑1 (MQ=255)
                                                                            cccACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGTCCTCCGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCg                                                                     <  1:971256/150‑1 (MQ=255)
                                                                             ccACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCgg                                                                    >  2:1612826/1‑150 (MQ=255)
                                                                                cTGCAACTGTTTGGTTTCCATTTATAATCCCGTACGCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGACTGCCGTCAGTAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGACAACGGCGATATGGTGCGGgtg                                                                 <  1:88692/150‑1 (MQ=255)
                                                                                  gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                               <  1:1062204/150‑1 (MQ=255)
                                                                                  gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                               >  1:1071185/1‑150 (MQ=255)
                                                                                  gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGTGCGGGTGttt                                                              >  1:734282/1‑150 (MQ=255)
                                                                                   cAACTGTTTGGTTTCCATTACAAATCCCTTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCGGCCTGCCGTCAGGAGGTGTGTATCAACCCTATAGATGCGCAGAAACGTGTGATTGCCAACGGCGATATGGTGCGGGTGttt                                                              <  1:1827168/150‑1 (MQ=255)
                                                                                   cAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGTTCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGttt                                                              >  2:778768/1‑150 (MQ=255)
                                                                                    aaCTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTa                                                             <  2:644124/150‑1 (MQ=255)
                                                                                      cTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAAc                                                           >  2:1543882/1‑150 (MQ=255)
                                                                                      cTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAAc                                                           <  2:1467051/150‑1 (MQ=255)
                                                                                        gTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCa                                                         >  1:783168/1‑150 (MQ=255)
                                                                                          ttGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACACTATAGATGCGCAGAAACGTGGGATTGCCGACGGCGATATGGTGCGGGTGTTTAACCATc                                                       >  1:711551/1‑150 (MQ=255)
                                                                                            ggTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGt                                                     >  2:1389759/1‑150 (MQ=255)
                                                                                             gTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTg                                                    >  2:1012863/1‑150 (MQ=255)
                                                                                              tttCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCATCCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTgg                                                   <  1:322811/150‑1 (MQ=255)
                                                                                                   aTTACAAATCCCGTACTCACTCGACCTACGGCAATATTGTTCTCCTGTAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCTGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAg                                              <  1:34294/150‑1 (MQ=255)
                                                                                                   aTTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAg                                              >  1:672788/1‑150 (MQ=255)
                                                                                                   aTTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAg                                              >  1:1060791/1‑150 (MQ=255)
                                                                                                     tACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGtt                                            >  1:189225/1‑150 (MQ=255)
                                                                                                       ctaaTCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAACCGTGGGATTGCCAACGGCGATATGGTGCGCGTGTTTAACCATCGTGGCGAAGTTCg                                          >  1:773785/3‑150 (MQ=255)
                                                                                                       cAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCg                                          >  2:825068/1‑150 (MQ=255)
                                                                                                                tACTCACTCGACCTACGGCAATATAGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGAATGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGc                                 <  2:1009364/150‑1 (MQ=255)
                                                                                                                 actcactcGACCTCCGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGGGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGcc                                <  2:372712/150‑1 (MQ=255)
                                                                                                                   tcactcGACCTCCGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCaa                              <  1:534084/150‑1 (MQ=255)
                                                                                                                    cactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCaaa                             >  1:10630/1‑150 (MQ=255)
                                                                                                                         gagcTACGGCATTATTGATCTGCTGAAGGCTGCCTGCCGTCAGGAGTTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGGGGCGAAGTTCGGCTACCAGCCAAAGTAac                        <  2:109998/147‑1 (MQ=255)
                                                                                                                         gACCTAGGGCAATATTGATCTCCTGAAGGCTGCCTTCCGTCAGGAGGTGTGGATCAACCCTATCGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAac                        <  1:702370/150‑1 (MQ=255)
                                                                                                                            cTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAAcacc                     >  1:1285901/1‑150 (MQ=255)
                                                                                                                             tACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAAcacca                    <  1:621254/150‑1 (MQ=255)
                                                                                                                               cGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACg                  >  1:385498/1‑150 (MQ=255)
                                                                                                                                  cAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTAt               <  1:1476218/150‑1 (MQ=255)
                                                                                                                                     tatTGATCTCCTGACGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATtct            <  2:1635325/150‑1 (MQ=255)
                                                                                                                                     tatTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATtct            >  1:1038668/1‑150 (MQ=255)
                                                                                                                                       cacacctcCTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTcc          >  1:967342/6‑150 (MQ=255)
                                                                                                                                         gATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCt        <  1:1543882/150‑1 (MQ=255)
                                                                                                                                         gATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCt        <  1:448236/150‑1 (MQ=255)
                                                                                                                                         cacatcCTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCt        >  2:757076/5‑150 (MQ=255)
                                                                                                                                          aTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTg       >  1:235631/1‑150 (MQ=255)
                                                                                                                                          aTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTg       >  2:1439369/1‑150 (MQ=255)
                                                                                                                                           cctcCTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACCATATAGAAGCGCAGAAACGTGAGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTgg      >  2:645895/2‑150 (MQ=255)
                                                                                                                                             tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGTATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCTGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg    <  1:811865/150‑1 (MQ=255)
                                                                                                                                             tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTTGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg    <  1:818798/150‑1 (MQ=255)
                                                                                                                                             tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg    >  1:867926/1‑150 (MQ=255)
                                                                                                                                              ccTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt   >  1:956910/1‑150 (MQ=255)
                                                                                                                                               cTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGGGGGCTTGCCACCGGCGATATGGTGCGGGTGTTTACCCATCGTGGCGAAGGTCGGCTACCCGCCAAAGTAACACCACGTCTTCTCCCTGGAGtt  >  1:1732226/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
AAGATGAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTT  >  NC_000913/1660047‑1660339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: