Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,660,200 C→G 8.8% A711G (GCC→GGC)  ynfE → putative selenate reductase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,660,2000CG 8.8% 292.9 / 12.8 97A711G (GCC→GGC) ynfEputative selenate reductase, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (42/44);  new base G (3/6);  total (46/51)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

GAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGG  >  NC_000913/1660052‑1660349
                                                                                                                                                    |                                                                                                                                                     
gAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGctgcc                                                                                                                                                      >  2:1707781/1‑150 (MQ=255)
gAAGTGATAAGCCCATTGCCAGTTTACGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGctgcc                                                                                                                                                      <  1:1298096/150‑1 (MQ=255)
     gaaaatcccATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCACTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCAATTACAAATCCCGTACTCACTCCACCTACGGGAATATTGAACTCCTGAAGGCTGCCTGCCg                                                                                                                                                 >  1:73737/7‑150 (MQ=255)
        aaGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCa                                                                                                                                              >  1:644124/1‑150 (MQ=255)
         aGCCCATTGCCAGTTTATGCCTAAACCTTAGAAGGCAGGAACTCCCCTGAACGTAGAACCTTCCCCCTGCAACTGTTTGGGTTCCATTACAAAACCCGTACTAACACGCCCAACGCCAATATTGAACTCCTGAAGGATGCCTGCCGTCac                                                                                                                                             >  1:1848087/1‑149 (MQ=255)
          gCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCagg                                                                                                                                            <  1:745073/150‑1 (MQ=255)
           cccATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGACGGCAGGCAGCCTTCagga                                                                                                                                           <  1:1070638/150‑1 (MQ=255)
            ccATTGCCCGTTTATGCCTCAACCTTTGAAGGCGGGAACTCCCTTTAACTTATATACTTCCCACTGCAACTGTTTGGTTTACATTACAAATCACGTACTCACTCGACCTACGGCAATATTGCTCTCCTGACGGCAGGCAGCCTTCaggag                                                                                                                                          <  2:343255/150‑1 (MQ=255)
            ccATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGACGGCAGGCAGCCTTCaggag                                                                                                                                          <  1:409824/150‑1 (MQ=255)
             cATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCaggagg                                                                                                                                         >  1:23696/1‑150 (MQ=255)
                tGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGgtgt                                                                                                                                      >  2:691438/1‑150 (MQ=255)
                tGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGACCTCCTGACGGCAGGCAGCCTTCaggagatca                                                                                                                                      <  1:426823/150‑5 (MQ=255)
                tGCCAGTATATGCCTCAACCTTTGACGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTGTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGgtgt                                                                                                                                      <  1:959172/150‑1 (MQ=255)
                  ccAGTTTATGCCTCATCCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTgg                                                                                                                                    <  2:269727/150‑1 (MQ=255)
                  ccAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGACGGCAGGCAGCCTTCaggagatcaat                                                                                                                                    <  1:1061650/150‑7 (MQ=255)
                   cAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGCTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGa                                                                                                                                   <  1:1410483/150‑1 (MQ=255)
                      tttATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCa                                                                                                                                <  1:1517406/150‑1 (MQ=255)
                      tttATGCCTCAACCGTTGACGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCCACTCGTTGCGTTCCATTACAAATCCCCTCCTCACACGCCCTACGGCAATCATGATCTCCTGACGGCTGCCTGCCGTCAGGAGGTGTGGATCa                                                                                                                                <  2:120735/150‑1 (MQ=255)
                        tATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAAc                                                                                                                              <  1:664489/150‑1 (MQ=255)
                         aTGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTATCCATTACAAATCCCGTACTCACTCGACCTACCGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGAGGATAAAcc                                                                                                                             >  1:109998/1‑150 (MQ=255)
                            ccacAACCATTGAAGGCTGGAACTCCCCTGAACGTACAACCTTCCCACTGCATCTGTTTGGTTTCAATTACAAATCCCGTACTCACTCGACCTACGCCAATATTGATCTCCTGAAGGATGACTGCCGACAGGAGGTGTGGATCAACCCta                                                                                                                          >  2:812366/4‑150 (MQ=255)
                            ccTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCAATCGTCCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGGGTGGATCACCCCta                                                                                                                          >  1:920376/1‑150 (MQ=255)
                              tCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCtata                                                                                                                        <  1:993475/150‑1 (MQ=255)
                               ccaCCTTTGTAGGCTGGAACTCCCGTGAACGTAGAACCTTCCCACTGCTACTGTTTGGTTTCCTTTACAAATCCCGTACTCTCTCGACATACGTCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAg                                                                                                                       <  1:360948/148‑1 (MQ=255)
                               cAACCTTTGACGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTTCTCACTCGACCTACGGCAATATTGATCTCCTCACGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAg                                                                                                                       <  2:387092/150‑1 (MQ=255)
                                  ccTTTGAAGGCTGGAACTCCCCTGAACGTCGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATg                                                                                                                    <  2:516937/150‑1 (MQ=255)
                                  ccTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATg                                                                                                                    >  1:634516/1‑150 (MQ=255)
                                  ccTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGGGTGGATCAACCCTATAGATg                                                                                                                    >  2:843399/1‑150 (MQ=255)
                                      tGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCa                                                                                                                <  2:1408047/150‑1 (MQ=255)
                                       gAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAg                                                                                                               >  2:448236/1‑150 (MQ=255)
                                               gAACTCCCCTGAACTTAGATCCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTgg                                                                                                       <  2:595295/150‑1 (MQ=255)
                                               gAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTgg                                                                                                       <  1:990408/150‑1 (MQ=255)
                                               gAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTgg                                                                                                       >  1:1385078/1‑150 (MQ=255)
                                               gAACACCACTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTgg                                                                                                       <  1:271207/150‑1 (MQ=255)
                                                aaCTCCCCTGAACGTAGAACCTACCCACTGCAACTGTTTGGTTTCCATTACAAATCCCTTACTCACTCGACATACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTggg                                                                                                      <  2:1126370/150‑1 (MQ=255)
                                                 aCTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAACGTGGGa                                                                                                     >  1:689425/1‑150 (MQ=255)
                                                   tCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGAtt                                                                                                   <  2:1812584/150‑1 (MQ=255)
                                                    ccccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTg                                                                                                  <  1:502975/150‑1 (MQ=255)
                                                    accatGAACGTAGAACCTTCCCACTGCAACTGATTGGGTTCCATTACAACTCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGGCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTg                                                                                                  <  1:277372/146‑1 (MQ=255)
                                                     cccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGc                                                                                                 <  2:129880/150‑1 (MQ=255)
                                                      ccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGcc                                                                                                <  1:691438/150‑1 (MQ=255)
                                                      ccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGcc                                                                                                >  2:1456414/1‑150 (MQ=255)
                                                           aCGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACgg                                                                                           <  1:1867988/150‑1 (MQ=255)
                                                            cGTAGAACCTTCCCCCTGCAACTGTTTGGTTTCCATTCCAACTCCCGAACTCACTCGACCTACGGCAATATTGAGCTCCTGAAGGCTGCATGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACggt                                                                                          <  2:45490/150‑2 (MQ=255)
                                                            cGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGc                                                                                          >  2:1457144/1‑150 (MQ=255)
                                                               cgAACCTGTCCAGTGCATCTGTTTGCTTTCCATTACAAATCCCGTACTCAATCTCCCTTTGGCAAAATTGTTGTCGTGTAGGCGGCCAGCCGTCAGGAGGTGGGGTTCAAGCGTAAATCTGCGCAGAAAGGTGGGATTGCCACCGGTGat                                                                                       <  2:1778852/149‑1 (MQ=255)
                                                               aGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGat                                                                                       >  2:252265/1‑150 (MQ=255)
                                                               aGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGACTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGat                                                                                       >  1:105228/1‑150 (MQ=255)
                                                                gAACCTTCCCACAGCAACTGTTTGGTTACAATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGata                                                                                      <  2:1170886/150‑1 (MQ=255)
                                                                       cccACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGTCCTCCGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCg                                                                               <  1:971256/150‑1 (MQ=255)
                                                                           cTGCAACTGTTTGGTTTCCATTTATAATCCCGTACGCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGACTGCCGTCAGTAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGACAACGGCGATATGGTGCGGgtg                                                                           <  1:88692/150‑1 (MQ=255)
                                                                             gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                         >  1:1071185/1‑150 (MQ=255)
                                                                             gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                         <  1:1062204/150‑1 (MQ=255)
                                                                             gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGTGCGGGTGttt                                                                        >  1:734282/1‑150 (MQ=255)
                                                                              cAACTGTTTGGTTTCCATTACAAATCCCTTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCGGCCTGCCGTCAGGAGGTGTGTATCAACCCTATAGATGCGCAGAAACGTGTGATTGCCAACGGCGATATGGTGCGGGTGttt                                                                        <  1:1827168/150‑1 (MQ=255)
                                                                              cAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGTTCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGttt                                                                        >  2:778768/1‑150 (MQ=255)
                                                                               aaCTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTa                                                                       <  2:644124/150‑1 (MQ=255)
                                                                                 cTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAAc                                                                     >  2:1543882/1‑150 (MQ=255)
                                                                                 cTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAAc                                                                     <  2:1467051/150‑1 (MQ=255)
                                                                                     ttGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACACTATAGATGCGCAGAAACGTGGGATTGCCGACGGCGATATGGTGCGGGTGTTTAACCATc                                                                 >  1:711551/1‑150 (MQ=255)
                                                                                       ggTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGt                                                               >  2:1389759/1‑150 (MQ=255)
                                                                                        gTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTg                                                              >  2:1012863/1‑150 (MQ=255)
                                                                                         tttCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCATCCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTgg                                                             <  1:322811/150‑1 (MQ=255)
                                                                                              aTTACAAATCCCGTACTCACTCGACCTACGGCAATATTGTTCTCCTGTAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCTGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAg                                                        <  1:34294/150‑1 (MQ=255)
                                                                                              aTTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAg                                                        >  1:1060791/1‑150 (MQ=255)
                                                                                              aTTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAg                                                        >  1:672788/1‑150 (MQ=255)
                                                                                                tACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGtt                                                      >  1:189225/1‑150 (MQ=255)
                                                                                                  ctaaTCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAACCGTGGGATTGCCAACGGCGATATGGTGCGCGTGTTTAACCATCGTGGCGAAGTTCg                                                    >  1:773785/3‑150 (MQ=255)
                                                                                                  cAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCg                                                    >  2:825068/1‑150 (MQ=255)
                                                                                                           tACTCACTCGACCTACGGCAATATAGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGAATGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGc                                           <  2:1009364/150‑1 (MQ=255)
                                                                                                            actcactcGACCTCCGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGGGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGcc                                          <  2:372712/150‑1 (MQ=255)
                                                                                                              tcactcGACCTCCGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCaa                                        <  1:534084/150‑1 (MQ=255)
                                                                                                               cactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCaaa                                       >  1:10630/1‑150 (MQ=255)
                                                                                                                    gagcTACGGCATTATTGATCTGCTGAAGGCTGCCTGCCGTCAGGAGTTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGGGGCGAAGTTCGGCTACCAGCCAAAGTAac                                  <  2:109998/147‑1 (MQ=255)
                                                                                                                    gACCTAGGGCAATATTGATCTCCTGAAGGCTGCCTTCCGTCAGGAGGTGTGGATCAACCCTATCGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAac                                  <  1:702370/150‑1 (MQ=255)
                                                                                                                       cTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAAcacc                               >  1:1285901/1‑150 (MQ=255)
                                                                                                                        tACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAAcacca                              <  1:621254/150‑1 (MQ=255)
                                                                                                                          cGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACg                            >  1:385498/1‑150 (MQ=255)
                                                                                                                             cAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTAt                         <  1:1476218/150‑1 (MQ=255)
                                                                                                                                tatTGATCTCCTGACGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATtct                      <  2:1635325/150‑1 (MQ=255)
                                                                                                                                tatTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATtct                      >  1:1038668/1‑150 (MQ=255)
                                                                                                                                  cacacctcCTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTcc                    >  1:967342/6‑150 (MQ=255)
                                                                                                                                    gATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCt                  <  1:448236/150‑1 (MQ=255)
                                                                                                                                    gATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCt                  <  1:1543882/150‑1 (MQ=255)
                                                                                                                                    cacatcCTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCt                  >  2:757076/5‑150 (MQ=255)
                                                                                                                                     aTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTg                 >  2:1439369/1‑150 (MQ=255)
                                                                                                                                     aTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTg                 >  1:235631/1‑150 (MQ=255)
                                                                                                                                      cctcCTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACCATATAGAAGCGCAGAAACGTGAGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTgg                >  2:645895/2‑150 (MQ=255)
                                                                                                                                        tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGTATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCTGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg              <  1:811865/150‑1 (MQ=255)
                                                                                                                                        tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTTGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg              <  1:818798/150‑1 (MQ=255)
                                                                                                                                        tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg              >  1:867926/1‑150 (MQ=255)
                                                                                                                                         ccTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt             >  1:956910/1‑150 (MQ=255)
                                                                                                                                          cTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGGGGGCTTGCCACCGGCGATATGGTGCGGGTGTTTACCCATCGTGGCGAAGGTCGGCTACCCGCCAAAGTAACACCACGTCTTCTCCCTGGAGtt            >  1:1732226/1‑150 (MQ=255)
                                                                                                                                                gCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTTCCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCt      <  2:189225/150‑1 (MQ=255)
                                                                                                                                                gCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCt      >  1:1626019/1‑150 (MQ=255)
                                                                                                                                                 ctgcctgcCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTa     >  1:1494937/1‑150 (MQ=255)
                                                                                                                                                   gcctgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGTTTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATg   <  2:23696/150‑1 (MQ=255)
                                                                                                                                                   gcctgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATg   <  1:719856/150‑1 (MQ=255)
                                                                                                                                                   gcctgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATg   <  2:246721/150‑1 (MQ=255)
                                                                                                                                                    cctgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATgg  >  2:1729856/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GAAGTGATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGG  >  NC_000913/1660052‑1660349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: