Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,660,206 G→T 7.1% R713L (CGT→CTT)  ynfE → putative selenate reductase, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,660,2060GT 7.1% 302.3 / 9.5 106R713L (CGT→CTT) ynfEputative selenate reductase, periplasmic
Reads supporting (aligned to +/- strand):  ref base G (48/50);  new base T (3/5);  total (51/55)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.55e-01

Only 100 of 107 total aligned reads displayed.

GATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAG  >  NC_000913/1660057‑1660354
                                                                                                                                                     |                                                                                                                                                    
gaaaatcccATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCACTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCAATTACAAATCCCGTACTCACTCCACCTACGGGAATATTGAACTCCTGAAGGCTGCCTGCCg                                                                                                                                                      >  1:73737/7‑150 (MQ=255)
   aaGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCa                                                                                                                                                   >  1:644124/1‑150 (MQ=255)
    aGCCCATTGCCAGTTTATGCCTAAACCTTAGAAGGCAGGAACTCCCCTGAACGTAGAACCTTCCCCCTGCAACTGTTTGGGTTCCATTACAAAACCCGTACTAACACGCCCAACGCCAATATTGAACTCCTGAAGGATGCCTGCCGTCac                                                                                                                                                  >  1:1848087/1‑149 (MQ=255)
     gCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCagg                                                                                                                                                 <  1:745073/150‑1 (MQ=255)
      cccATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGACGGCAGGCAGCCTTCagga                                                                                                                                                <  1:1070638/150‑1 (MQ=255)
       ccATTGCCCGTTTATGCCTCAACCTTTGAAGGCGGGAACTCCCTTTAACTTATATACTTCCCACTGCAACTGTTTGGTTTACATTACAAATCACGTACTCACTCGACCTACGGCAATATTGCTCTCCTGACGGCAGGCAGCCTTCaggag                                                                                                                                               <  2:343255/150‑1 (MQ=255)
       ccATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGACGGCAGGCAGCCTTCaggag                                                                                                                                               <  1:409824/150‑1 (MQ=255)
        cATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCaggagg                                                                                                                                              >  1:23696/1‑150 (MQ=255)
           tGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGgtgt                                                                                                                                           >  2:691438/1‑150 (MQ=255)
           tGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGACCTCCTGACGGCAGGCAGCCTTCaggagatca                                                                                                                                           <  1:426823/150‑5 (MQ=255)
             ccAGTTTATGCCTCATCCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTgg                                                                                                                                         <  2:269727/150‑1 (MQ=255)
             ccAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGACGGCAGGCAGCCTTCaggagatcaat                                                                                                                                         <  1:1061650/150‑7 (MQ=255)
              cAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGCTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGa                                                                                                                                        <  1:1410483/150‑1 (MQ=255)
                 tttATGCCTCAACCGTTGACGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCCACTCGTTGCGTTCCATTACAAATCCCCTCCTCACACGCCCTACGGCAATCATGATCTCCTGACGGCTGCCTGCCGTCAGGAGGTGTGGATCa                                                                                                                                     <  2:120735/150‑1 (MQ=255)
                   tATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAAc                                                                                                                                   <  1:664489/150‑1 (MQ=255)
                    aTGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTATCCATTACAAATCCCGTACTCACTCGACCTACCGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGAGGATAAAcc                                                                                                                                  >  1:109998/1‑150 (MQ=255)
                       ccacAACCATTGAAGGCTGGAACTCCCCTGAACGTACAACCTTCCCACTGCATCTGTTTGGTTTCAATTACAAATCCCGTACTCACTCGACCTACGCCAATATTGATCTCCTGAAGGATGACTGCCGACAGGAGGTGTGGATCAACCCta                                                                                                                               >  2:812366/4‑150 (MQ=255)
                       ccTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCta                                                                                                                               >  1:246721/1‑150 (MQ=255)
                       ccTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCAATCGTCCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGGGTGGATCACCCCta                                                                                                                               >  1:920376/1‑150 (MQ=255)
                         tCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCtata                                                                                                                             <  1:993475/150‑1 (MQ=255)
                          ccaCCTTTGTAGGCTGGAACTCCCGTGAACGTAGAACCTTCCCACTGCTACTGTTTGGTTTCCTTTACAAATCCCGTACTCTCTCGACATACGTCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAg                                                                                                                            <  1:360948/148‑1 (MQ=255)
                          cAACCTTTGACGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTTCTCACTCGACCTACGGCAATATTGATCTCCTCACGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAg                                                                                                                            <  2:387092/150‑1 (MQ=255)
                             ccTTTGAAGGCTGGAACTCCCCTGAACGTCGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATg                                                                                                                         <  2:516937/150‑1 (MQ=255)
                             ccTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATg                                                                                                                         >  1:634516/1‑150 (MQ=255)
                             ccTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGGGTGGATCAACCCTATAGATg                                                                                                                         >  2:843399/1‑150 (MQ=255)
                                 tGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCa                                                                                                                     <  2:1408047/150‑1 (MQ=255)
                                  gAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAg                                                                                                                    >  2:448236/1‑150 (MQ=255)
                                          gAACTCCCCTGAACTTAGATCCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTgg                                                                                                            <  2:595295/150‑1 (MQ=255)
                                          gAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTgg                                                                                                            <  1:990408/150‑1 (MQ=255)
                                          gAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTgg                                                                                                            >  1:1385078/1‑150 (MQ=255)
                                          gAACACCACTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTgg                                                                                                            <  1:271207/150‑1 (MQ=255)
                                           aaCTCCCCTGAACGTAGAACCTACCCACTGCAACTGTTTGGTTTCCATTACAAATCCCTTACTCACTCGACATACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTggg                                                                                                           <  2:1126370/150‑1 (MQ=255)
                                            aCTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCACCCCTATAGATGCGCAGAAACGTGGGa                                                                                                          >  1:689425/1‑150 (MQ=255)
                                              tCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGAtt                                                                                                        <  2:1812584/150‑1 (MQ=255)
                                               ccccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTg                                                                                                       <  1:502975/150‑1 (MQ=255)
                                               accatGAACGTAGAACCTTCCCACTGCAACTGATTGGGTTCCATTACAACTCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGGCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTg                                                                                                       <  1:277372/146‑1 (MQ=255)
                                                cccTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGc                                                                                                      <  2:129880/150‑1 (MQ=255)
                                                      aCGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACgg                                                                                                <  1:1867988/150‑1 (MQ=255)
                                                       cGTAGAACCTTCCCCCTGCAACTGTTTGGTTTCCATTCCAACTCCCGAACTCACTCGACCTACGGCAATATTGAGCTCCTGAAGGCTGCATGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACggt                                                                                               <  2:45490/150‑2 (MQ=255)
                                                       cGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGc                                                                                               >  2:1457144/1‑150 (MQ=255)
                                                          cgAACCTGTCCAGTGCATCTGTTTGCTTTCCATTACAAATCCCGTACTCAATCTCCCTTTGGCAAAATTGTTGTCGTGTAGGCGGCCAGCCGTCAGGAGGTGGGGTTCAAGCGTAAATCTGCGCAGAAAGGTGGGATTGCCACCGGTGat                                                                                            <  2:1778852/149‑1 (MQ=255)
                                                          aGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGat                                                                                            >  2:252265/1‑150 (MQ=255)
                                                          aGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGACTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGat                                                                                            >  1:105228/1‑150 (MQ=255)
                                                           gAACCTTCCCACAGCAACTGTTTGGTTACAATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGata                                                                                           <  2:1170886/150‑1 (MQ=255)
                                                                  cccACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGTCCTCCGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCg                                                                                    <  1:971256/150‑1 (MQ=255)
                                                                   ccACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCgg                                                                                   >  2:1612826/1‑150 (MQ=255)
                                                                      cTGCAACTGTTTGGTTTCCATTTATAATCCCGTACGCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGACTGCCGTCAGTAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGACAACGGCGATATGGTGCGGgtg                                                                                <  1:88692/150‑1 (MQ=255)
                                                                        gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                              <  1:1062204/150‑1 (MQ=255)
                                                                        gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGtt                                                                              >  1:1071185/1‑150 (MQ=255)
                                                                        gCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGTGCGGGTGttt                                                                             >  1:734282/1‑150 (MQ=255)
                                                                         cAACTGTTTGGTTTCCATTACAAATCCCTTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCGGCCTGCCGTCAGGAGGTGTGTATCAACCCTATAGATGCGCAGAAACGTGTGATTGCCAACGGCGATATGGTGCGGGTGttt                                                                             <  1:1827168/150‑1 (MQ=255)
                                                                         cAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGTTCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGttt                                                                             >  2:778768/1‑150 (MQ=255)
                                                                          aaCTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTa                                                                            <  2:644124/150‑1 (MQ=255)
                                                                            cTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAAc                                                                          <  2:1467051/150‑1 (MQ=255)
                                                                            cTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAAc                                                                          >  2:1543882/1‑150 (MQ=255)
                                                                              gTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCa                                                                        >  1:783168/1‑150 (MQ=255)
                                                                                ttGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACACTATAGATGCGCAGAAACGTGGGATTGCCGACGGCGATATGGTGCGGGTGTTTAACCATc                                                                      >  1:711551/1‑150 (MQ=255)
                                                                                  ggTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGt                                                                    >  2:1389759/1‑150 (MQ=255)
                                                                                   gTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTg                                                                   >  2:1012863/1‑150 (MQ=255)
                                                                                         aTTACAAATCCCGTACTCACTCGACCTACGGCAATATTGTTCTCCTGTAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCTGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAg                                                             <  1:34294/150‑1 (MQ=255)
                                                                                         aTTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAg                                                             >  1:672788/1‑150 (MQ=255)
                                                                                         aTTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAg                                                             >  1:1060791/1‑150 (MQ=255)
                                                                                           tACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGtt                                                           >  1:189225/1‑150 (MQ=255)
                                                                                             ctaaTCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAACCGTGGGATTGCCAACGGCGATATGGTGCGCGTGTTTAACCATCGTGGCGAAGTTCg                                                         >  1:773785/3‑150 (MQ=255)
                                                                                             cAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCg                                                         >  2:825068/1‑150 (MQ=255)
                                                                                                      tACTCACTCGACCTACGGCAATATAGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGAATGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGc                                                <  2:1009364/150‑1 (MQ=255)
                                                                                                         tcactcGACCTCCGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCaa                                             <  1:534084/150‑1 (MQ=255)
                                                                                                          cactcGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCaaa                                            >  1:10630/1‑150 (MQ=255)
                                                                                                               gagcTACGGCATTATTGATCTGCTGAAGGCTGCCTGCCGTCAGGAGTTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGGGGCGAAGTTCGGCTACCAGCCAAAGTAac                                       <  2:109998/147‑1 (MQ=255)
                                                                                                               gACCTAGGGCAATATTGATCTCCTGAAGGCTGCCTTCCGTCAGGAGGTGTGGATCAACCCTATCGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAac                                       <  1:702370/150‑1 (MQ=255)
                                                                                                                  cTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAAcacc                                    >  1:1285901/1‑150 (MQ=255)
                                                                                                                   tACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAAcacca                                   <  1:621254/150‑1 (MQ=255)
                                                                                                                     cGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACg                                 >  1:385498/1‑150 (MQ=255)
                                                                                                                        cAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTAt                              <  1:1476218/150‑1 (MQ=255)
                                                                                                                           tatTGATCTCCTGACGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATtct                           <  2:1635325/150‑1 (MQ=255)
                                                                                                                           tatTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATtct                           >  1:1038668/1‑150 (MQ=255)
                                                                                                                             cacacctcCTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTcc                         >  1:967342/6‑150 (MQ=255)
                                                                                                                               gATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCt                       <  1:448236/150‑1 (MQ=255)
                                                                                                                               gATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCt                       <  1:1543882/150‑1 (MQ=255)
                                                                                                                               cacatcCTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCt                       >  2:757076/5‑150 (MQ=255)
                                                                                                                                aTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTg                      >  2:1439369/1‑150 (MQ=255)
                                                                                                                                aTCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTg                      >  1:235631/1‑150 (MQ=255)
                                                                                                                                 cctcCTGACGGCAGGCAGCCTTCAGGAGGTGTGGATCAACCATATAGAAGCGCAGAAACGTGAGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTgg                     >  2:645895/2‑150 (MQ=255)
                                                                                                                                   tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGTATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCTGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg                   <  1:811865/150‑1 (MQ=255)
                                                                                                                                   tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTTGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg                   <  1:818798/150‑1 (MQ=255)
                                                                                                                                   tcCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAg                   >  1:867926/1‑150 (MQ=255)
                                                                                                                                    ccTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGt                  >  1:956910/1‑150 (MQ=255)
                                                                                                                                     cTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGGGGGCTTGCCACCGGCGATATGGTGCGGGTGTTTACCCATCGTGGCGAAGGTCGGCTACCCGCCAAAGTAACACCACGTCTTCTCCCTGGAGtt                 >  1:1732226/1‑150 (MQ=255)
                                                                                                                                           gCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTTCCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCt           <  2:189225/150‑1 (MQ=255)
                                                                                                                                           gCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCt           >  1:1626019/1‑150 (MQ=255)
                                                                                                                                            ctgcctgcCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTa          >  1:1494937/1‑150 (MQ=255)
                                                                                                                                              gcctgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGTTTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATg        <  2:23696/150‑1 (MQ=255)
                                                                                                                                              gcctgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATg        <  2:246721/150‑1 (MQ=255)
                                                                                                                                              gcctgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATg        <  1:719856/150‑1 (MQ=255)
                                                                                                                                               cctgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATgg       >  2:1729856/1‑150 (MQ=255)
                                                                                                                                                 tgccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGAGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGc     >  1:278427/1‑150 (MQ=255)
                                                                                                                                                  gccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGAGATGGTGCGGGGGTTTAACCATCGTGGCGAAGTTCGTCTACCAGCCAAAGTAACCCCACGGATTCTCCCAGGAGTGAGCGCTATGGGcc    >  2:463736/1‑150 (MQ=255)
                                                                                                                                                   ccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCa   >  1:1243515/1‑150 (MQ=255)
                                                                                                                                                   ccGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCa   >  1:1727764/1‑150 (MQ=255)
                                                                                                                                                    cGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAg  <  1:792991/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GATAAGCCCATTGCCAGTTTATGCCTCAACCTTTGAAGGCTGGAACTCCCCTGAACGTAGAACCTTCCCACTGCAACTGTTTGGTTTCCATTACAAATCCCGTACTCACTCGACCTACGGCAATATTGATCTCCTGAAGGCTGCCTGCCGTCAGGAGGTGTGGATCAACCCTATAGATGCGCAGAAACGTGGGATTGCCAACGGCGATATGGTGCGGGTGTTTAACCATCGTGGCGAAGTTCGGCTACCAGCCAAAGTAACACCACGTATTCTCCCTGGAGTTAGCGCTATGGGCCAG  >  NC_000913/1660057‑1660354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: