Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,666,470 C→A 5.2% L386I (CTA→ATA)  clcB → H(+)/Cl(‑) exchange transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,666,4700CA 5.2% 383.5 / 7.3 128L386I (CTA→ATA) clcBH(+)/Cl(‑) exchange transporter
Reads supporting (aligned to +/- strand):  ref base C (50/71);  new base A (3/4);  total (53/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 129 total aligned reads displayed.

TGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCAGATAAGG  >  NC_000913/1666322‑1666619
                                                                                                                                                    |                                                                                                                                                     
tGGTCGTATCTTGGGATGATGGCTCCCTGATGTCGAAGATATTACACGTTTCCTCGGATTGACCGGGATGGAGACTCTGGAGCCGGCTACCACGCACGCGCCGATTATGTAGACGGTGAGGATATGTGAAATGACCTGGGAGTATCAGCt                                                                                                                                                      <  1:1163040/150‑1 (MQ=255)
 ggTCTTAGACTGGGATTATGGTTACCTGATGCCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGGGACACTGTTGGCGGCGACCACGCACGCTCCGATTATGTCGACGTTGATGATATGAGAAATGACCGGGGAGTATCAGCTa                                                                                                                                                     >  1:1145002/1‑150 (MQ=255)
  gTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTAc                                                                                                                                                    >  2:963206/1‑150 (MQ=255)
   tCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACt                                                                                                                                                   <  1:999697/150‑1 (MQ=255)
        gCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCCGCTACTCCCCg                                                                                                                                              >  1:901759/1‑150 (MQ=255)
        gCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCg                                                                                                                                              >  1:1049287/1‑150 (MQ=255)
        gCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCg                                                                                                                                              >  1:127611/1‑150 (MQ=255)
          cTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGt                                                                                                                                            >  1:1435961/1‑150 (MQ=255)
           tGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGtt                                                                                                                                           <  1:391356/150‑1 (MQ=255)
           tGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGtt                                                                                                                                           >  1:528732/1‑150 (MQ=255)
           tGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGtt                                                                                                                                           <  1:1599774/150‑1 (MQ=255)
           tGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGtt                                                                                                                                           <  1:79615/150‑1 (MQ=255)
                ttATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCTACGACGCACGCGCCGATTATGTCGACGTTGATGATATGTGATATGACCGGGGAGTATCAGCTACTCCCCGGTTTattg                                                                                                                                      <  1:1241107/150‑1 (MQ=255)
                  aTGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattgat                                                                                                                                    <  2:630178/150‑1 (MQ=255)
                  aTGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattgat                                                                                                                                    >  2:565012/1‑150 (MQ=255)
                  aTGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGAGATGTGAAATGACAGGGGAGTATCAGCTACTCCCCGGTGTattgat                                                                                                                                    >  2:127233/1‑150 (MQ=255)
                   tGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattgatt                                                                                                                                   <  1:185538/150‑1 (MQ=255)
                   tGGTTCCCTGATGGCGAAGAAATTACACTTTAACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTAGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTaatgatt                                                                                                                                   >  1:640225/1‑150 (MQ=255)
                    ggTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattgattg                                                                                                                                  <  2:611981/150‑1 (MQ=255)
                    ggTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCAGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTattgattg                                                                                                                                  >  1:18799/1‑150 (MQ=255)
                     gTTCCCTGATGGCGAAGAAA‑TACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTTCTCCCCGGTTTATTGATTGcc                                                                                                                                >  1:444868/1‑150 (MQ=255)
                      ttCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGcc                                                                                                                                <  1:14586/150‑1 (MQ=255)
                          cTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATGAGCTACTCCCCGGTTTATTGATTGCCTGCg                                                                                                                            <  1:102213/150‑1 (MQ=255)
                              tGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGGTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAAt                                                                                                                        >  2:414811/1‑150 (MQ=255)
                                 cGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGc                                                                                                                     >  2:557015/1‑150 (MQ=255)
                                      aaaTTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCgg                                                                                                                <  2:1610983/150‑1 (MQ=255)
                                         ttACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTaa                                                                                                             >  2:470237/1‑150 (MQ=255)
                                                tttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgcg                                                                                                      <  1:535598/150‑1 (MQ=255)
                                                tttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgcg                                                                                                      <  2:1424461/150‑1 (MQ=255)
                                                tttACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTcgcg                                                                                                      <  1:1362497/150‑1 (MQ=255)
                                                      cGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGtt                                                                                                <  1:337901/150‑1 (MQ=255)
                                                         aTTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTaca                                                                                             >  2:1747011/1‑150 (MQ=255)
                                                           tGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACAcc                                                                                           >  1:1097650/1‑150 (MQ=255)
                                                              ccGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTg                                                                                        <  1:1650762/150‑1 (MQ=255)
                                                               cGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTAGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTAGCGGACGTTACACCGTgc                                                                                       >  1:1357857/1‑149 (MQ=255)
                                                                 ggATGGCGCCACTTTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGAct                                                                                     <  2:292535/150‑1 (MQ=255)
                                                                   aTGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGGTGATGATAAGTGAAATGCCCCGCGAGGATCAGCTACTCCCCGGTTTATTGATTGCATGCGTAATTGCGTCGGGAAGTAGGCGGCCGTTACCCCGTGActct                                                                                   >  2:371970/1‑150 (MQ=255)
                                                                    tGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAATTGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTAACCCGTGACtcta                                                                                  >  2:1109825/1‑150 (MQ=255)
                                                                    tGGCGACACTGTTGGCGGCGACCACGCACGCGCCGAGTATGTCGACGGTGAGGATATGTGAAATGACCGGGGAGTATCAGCGACTCCCCGGTTTATTGATTGCCTGCGTTATTGCGTCGGGAAGTTCGCGGACGTTACACCGTGACtcta                                                                                  >  2:1103235/1‑150 (MQ=255)
                                                                     ggCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctat                                                                                 <  1:988088/150‑1 (MQ=255)
                                                                     ggCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctat                                                                                 <  1:1617118/150‑1 (MQ=255)
                                                                     ggCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctat                                                                                 <  1:609080/150‑1 (MQ=255)
                                                                     ggCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACgctat                                                                                 <  1:1231335/150‑1 (MQ=255)
                                                                      gggACAGTGTTGGCGGCGACCACTCACGCGCTGTTTTTGTCGACGTTGATGATATGTGAAATTACGGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTTACtctaac                                                                                <  1:1737276/148‑3 (MQ=255)
                                                                       cGACACTGTTGGCGGCGACCACGCACGCGCCTATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatct                                                                               <  2:1115238/150‑1 (MQ=255)
                                                                       cGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACtctatct                                                                               <  2:8967/150‑1 (MQ=255)
                                                                         acacTTTTGGCGTCGACCAAGCACTCGCCGATTATGTCGAAGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTCCTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTAc                                                                             <  2:1561796/150‑1 (MQ=255)
                                                                           acTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCg                                                                           <  1:670289/150‑1 (MQ=255)
                                                                             tGTTGGCCGCTACCACGCACGCGCCGATTATTTCGACCTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGcc                                                                         <  2:338600/150‑1 (MQ=255)
                                                                              gTTGGCGGCGACCACGCACGCGCGGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTGGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCa                                                                        >  1:96312/1‑150 (MQ=255)
                                                                              gTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCa                                                                        <  2:127611/150‑1 (MQ=255)
                                                                               ttGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATCTGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAg                                                                       <  1:906652/150‑1 (MQ=255)
                                                                                tGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGTAGTATCAGCGACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGc                                                                      <  2:898872/150‑1 (MQ=255)
                                                                                tGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGc                                                                      <  2:1606936/150‑1 (MQ=255)
                                                                                     gcgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACt                                                                 >  1:1843540/1‑150 (MQ=255)
                                                                                     gcgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTACCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCCATCTACCGCCAGCACACt                                                                 >  1:1461724/1‑150 (MQ=255)
                                                                                      cgACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTg                                                                >  2:955235/1‑150 (MQ=255)
                                                                                      cgACCACGCACGCGCCGATTATGTCGACGGTGATGATATGTGAAATGACCGGGGAGTATCAGCAACTCCACGGATTATTGATTCCCAGCGTAATTGCGTCGGTAATTTCGCGGACTTTACGCCGAGACACGAACAACCGCCCCCacacgg                                                                >  2:132353/1‑148 (MQ=255)
                                                                                         ccACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTgcgc                                                             <  1:963206/150‑1 (MQ=255)
                                                                                          cacgcacgCGCCGATTATGGCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgca                                                            <  2:498565/150‑1 (MQ=255)
                                                                                           acgcacgcGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCgcag                                                           <  1:22283/150‑1 (MQ=255)
                                                                                                cgcgCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATGTACCGCCCGCACACTGCGCAGCATAg                                                      >  1:477526/1‑150 (MQ=255)
                                                                                                   gcCGAATATGTCGACGTTGATGATATGTGAAATGACCGGGGAGGATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTACTTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCTCCAGCACACTGCGCAGCATAGCTa                                                   <  1:127233/150‑1 (MQ=255)
                                                                                                    ccGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTAGCGGCCGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTaa                                                  >  1:955461/1‑150 (MQ=255)
                                                                                                      gATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGAGTAATTCCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGc                                                >  2:331577/1‑150 (MQ=255)
                                                                                                      gATTATGTCGACGGTGATGATCTGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGc                                                <  2:537119/150‑1 (MQ=255)
                                                                                                       aTTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCg                                               <  1:1654034/150‑1 (MQ=255)
                                                                                                        ttATGTCGACGTTGATGATATGTGAAATGACCGGGTAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCgg                                              <  1:1043925/150‑1 (MQ=255)
                                                                                                         tATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGa                                             <  2:1305152/150‑1 (MQ=255)
                                                                                                         tATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGa                                             <  1:407931/150‑1 (MQ=255)
                                                                                                         tATGTCGACGTTGAAGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAAATTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGa                                             <  1:1503250/150‑1 (MQ=255)
                                                                                                           tGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATg                                           <  2:503213/150‑1 (MQ=255)
                                                                                                           tGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATg                                           <  1:1689650/150‑1 (MQ=255)
                                                                                                           tGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATg                                           <  1:1747011/150‑1 (MQ=255)
                                                                                                                aCGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTg                                      <  1:954826/150‑1 (MQ=255)
                                                                                                                    tgatgaTATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGcccc                                  <  2:161096/150‑1 (MQ=255)
                                                                                                                    tgatgaTATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGcccc                                  <  2:1393789/150‑1 (MQ=255)
                                                                                                                    tgatgaTATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGcccc                                  <  2:671397/150‑1 (MQ=255)
                                                                                                                      ttgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATATCTAAGCGGATGTATTGCCCCAg                                <  1:645826/149‑1 (MQ=255)
                                                                                                                      atgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAg                                >  2:488507/1‑150 (MQ=255)
                                                                                                                       tgatATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGt                               <  1:1720805/150‑1 (MQ=255)
                                                                                                                         ttatGGGAACTGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGGGGACGTTACACCGTGACTCTATCTCCCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTc                             <  1:1208035/149‑1 (MQ=255)
                                                                                                                         atatGGGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGTCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTc                             <  2:1692547/150‑1 (MQ=255)
                                                                                                                           atGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCAc                           <  2:1049287/150‑1 (MQ=255)
                                                                                                                            tgtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCAcc                          >  1:1119527/1‑149 (MQ=255)
                                                                                                                            tgtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACg                          <  2:901759/150‑1 (MQ=255)
                                                                                                                            tgtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACg                          >  1:612525/1‑150 (MQ=255)
                                                                                                                             gtgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGc                         >  1:343601/1‑150 (MQ=255)
                                                                                                                              tgAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCt                        <  2:945000/150‑1 (MQ=255)
                                                                                                                              tgAAATCTCCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCt                        <  1:275543/150‑1 (MQ=255)
                                                                                                                                   aaaCCGGGGAGTAGCTGATACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCg                   >  1:1024051/3‑150 (MQ=255)
                                                                                                                                    gACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCgg                  >  2:678326/1‑150 (MQ=255)
                                                                                                                                      ccGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGgcg                <  1:470237/150‑1 (MQ=255)
                                                                                                                                      ccGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCGGTTCGgcg                >  1:677256/1‑150 (MQ=255)
                                                                                                                                           gAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATCGCGTCGGGAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCAGAGCTAAGCGGATGTATTGCCCCAGTTCACGCAGTTCGGCGCGCgg           >  2:294467/1‑150 (MQ=255)
                                                                                                                                             gTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTATTTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCa         <  2:1706387/150‑1 (MQ=255)
                                                                                                                                              tATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCAg        <  1:691573/150‑1 (MQ=255)
                                                                                                                                              tATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCAg        <  2:18799/150‑1 (MQ=255)
                                                                                                                                               aTCAGCTACTCCCCCGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCACAGTTCACGCTCTTCGGCGCGCGGCAGa       >  1:1499802/1‑150 (MQ=255)
                                                                                                                                                   gCTACTCCCCGTTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTACGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCAGATAAg   <  2:1145002/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGGTCGTAGCCTGGGATTATGGTTCCCTGATGGCGAAGAAATTACACTTTTACTCGGATTGACCGGGATGGCGACACTGTTGGCGGCGACCACGCACGCGCCGATTATGTCGACGTTGATGATATGTGAAATGACCGGGGAGTATCAGCTACTCCCCGGTTTATTGATTGCCTGCGTAATTGCGTCGGTAATTTCGCGGACGTTACACCGTGACTCTATCTACCGCCAGCACACTGCGCAGCATAGCTAAGCGGATGTATTGCCCCAGTTCACGCTGTTCGGCGCGCGGCAGATAAGG  >  NC_000913/1666322‑1666619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑