Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,708,709 T→A 22.8% R597R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,7090TA22.8% 48.6 / 3.8 40R597R (CGT→CGArsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base T (4/24);  new base A (3/4);  total (8/32)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAG  >  NC_000913/1708561‑1708847
                                                                                                                                                     |                                                                                                                                          
cagcaACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTg                                                                                                                                           <  2:778749/150‑1 (MQ=255)
cagcaACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTg                                                                                                                                           <  1:1037096/150‑1 (MQ=255)
    aaCCGTTGCTGAGCCCCGTAAAACTGCCGTTGTAGCAGCTATCGCCCGCGCCAACGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGCACCACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCaa                                                                                                                                       <  2:307632/150‑1 (MQ=17)
      aacatGCCGATCCCCGCAAACCTGCAGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCTCAAGCTTGAACAGCATCAGGCTAATGCGGTACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAg                                                                                                                                     <  2:1650275/146‑1 (MQ=11)
        gTTGCTGATCCACGTAAAACTGCCTGTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcg                                                                                                                                   <  1:436907/150‑1 (MQ=255)
        gTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcg                                                                                                                                   <  2:1752443/150‑1 (MQ=255)
          tGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGGCAAAgagcg                                                                                                                                 >  1:1650275/1‑150 (MQ=255)
          tGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgcg                                                                                                                                 <  1:683630/150‑1 (MQ=255)
              taTCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAg                                                                                                                             <  1:127404/149‑1 (MQ=255)
                 ccACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCAGGTAGGAAAAGGCGCTCCGAGCGAAACAGCCGAAGAAGCAGGTTTTGCCCGTGCAACAGCGCGCaatata                                                                                                                          >  2:1492457/1‑146 (MQ=12)
                 ccACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTg                                                                                                                          <  1:705294/150‑1 (MQ=255)
                  cACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTgg                                                                                                                         <  2:1794308/150‑1 (MQ=255)
                  cACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTgg                                                                                                                         <  2:1354900/150‑1 (MQ=255)
                   ccGTAAAACTGCCTTTGAAGCAGCTAGCTCCCGCGCCAAAGCTCGCAAGCTGGAACATCATCAGGCTAATTCGGAACCAGAACAACATGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGa                                                                                                                        <  1:1311565/149‑1 (MQ=17)
                     gTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAAc                                                                                                                      <  1:1765851/150‑1 (MQ=255)
                          aCTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCCGCAGTAGCAGCGGCTATTGCCCGCGCAAACGCCCGCAACCACGCACAGCac                                                                                                                 >  1:839106/1‑149 (MQ=14)
                            tGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAAAGGTAGGATCAGCGCACGGCTGACGTCGAGTCCGGAGTTGAGCGAGGCAAAGCGCGGAAGCTGGAAAAGAAACa                                                                                                               >  1:813606/1‑150 (MQ=9)
                              ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCACGCTGGCACAGCAACAgg                                                                                                             >  1:1405249/1‑150 (MQ=255)
                                  tGATGCAGCTCTCTCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTaa                                                                                                         <  1:1207031/150‑1 (MQ=255)
                                   ctagaagcTATCTCCCGTCCCAAAGCTCGCAAGCTGGAACATCATCAGGCTAATCAGGACCCAGCACAACAGGTCGATCCCCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAAt                                                                                                        <  1:326706/145‑1 (MQ=11)
                                      gcagcaATCGCCCGCCCCTAAGCGCGCAAGCTGGAACATCAACAGGCTAATGCGGAACCAGCACAACAGGGCGATCCCCGCAAAGCCGCCGTCGAAGCCGCTATGGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCg                                                                                                     <  1:337334/150‑1 (MQ=14)
                                                cccGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCTGAAGCAGAACAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCagaag                                                                                           <  2:1440803/150‑1 (MQ=14)
                                                     gcCTAAGCTCGGAAGCTGGAACATCAACAGGCTACTCCGGAAGCAGAACAACTTGTAGATCCGCGCAAATCCGCCGTAGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAg                                                                                      <  2:259689/150‑1 (MQ=14)
                                                      ccAAACCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAgg                                                                                     <  2:1566170/150‑1 (MQ=255)
                                                        cacccgcgcAAGCTGGAACAGCTACAGGATAATGCGGAACCAGAAACCGAGGGCGATCCCCGGAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTc                                                                                   <  2:62887/146‑1 (MQ=14)
                                                        aaatgtcgcAATCTGGAACATCAACAGGATAATTCGGAACCAGAAAAACATGCCGATCCTCGCAAATCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAATCAGAAGAACAGGTc                                                                                   <  1:1253784/144‑1 (MQ=14)
                                                               gcAAGCTGGAACAGCAACAGGCTAATGAGGAACCAGAACAACAGGTCGATCCTCGTAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACATCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCcgc                                                                            <  1:1679040/150‑1 (MQ=14)
                                                                  atcTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCa                                                                         <  1:1681520/148‑1 (MQ=255)
                                                                     tGGAACAGCTCCAGGCAACTTCGGAACCAGAACAACAGGCCGATCCTCGCAAACCCGCCGGCGCAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAg                                                                      <  2:1725055/150‑1 (MQ=14)
                                                                      ggAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGGAGGCTTGCGCAGCAGACGTATTAGGAGGAGCCATAAGAACAGGTAGATACGCGAAAAga                                                                     >  2:1679664/1‑149 (MQ=2)
                                                                            gCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCaa                                                                                                                                       <  1:480163/78‑1 (MQ=21)
                                                                            gCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCaa                                                                                                                                       >  2:480163/1‑78 (MQ=21)
                                                                              aaCAGGCTAATTCGGAACCAGAACAACAGGTCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCg                                                             <  2:1048366/150‑1 (MQ=255)
                                                                                 aGGCTAATCCGGACCCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                          <  2:577992/150‑1 (MQ=255)
                                                                                                  gAACAACAGGTCGATCCTCGCAAATCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGcc                                         <  2:1396984/150‑1 (MQ=255)
                                                                                                                 tCCTCGCAAAGCTGACGGCGAAGCCGCTATAGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt                           <  2:1703678/150‑1 (MQ=11)
                                                                                                                      gaaaaCCCGCCGTAGAAGCCGCTATTTCACGGGACAAATCGCGCAAGCTGGAACAGCAACAGGATACTGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAAc                      <  2:861786/148‑1 (MQ=11)
                                                                                                                                cgTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACCACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGc            <  1:1286829/150‑1 (MQ=255)
                                                                                                                                    gAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCAGCCGTCGATGCCGATATTGACCGTGCCAAAGCGCGAAATATGGAGCAGAAACCGGCTAAt        >  2:860230/1‑150 (MQ=11)
                                                                                                                                          gCTATTTCCCGCGCCAAACCTGGCAATCTGGAACACCAACAGCAAAATCCGGAACCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg  <  1:1714725/150‑1 (MQ=11)
                                                                                                                                                     |                                                                                                                                          
CAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAG  >  NC_000913/1708561‑1708847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: