Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,708,709 | T→A | 22.8% | R597R (CGT→CGA) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,708,709 | 0 | T | A | 22.8% | 48.6 / 3.8 | 40 | R597R (CGT→CGA) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
Reads supporting (aligned to +/- strand): ref base T (4/24); new base A (3/4); total (8/32) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.25e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAG > NC_000913/1708561‑1708847 | cagcaACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTg < 2:778749/150‑1 (MQ=255) cagcaACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTg < 1:1037096/150‑1 (MQ=255) aaCCGTTGCTGAGCCCCGTAAAACTGCCGTTGTAGCAGCTATCGCCCGCGCCAACGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGCACCACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCaa < 2:307632/150‑1 (MQ=17) aacatGCCGATCCCCGCAAACCTGCAGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCTCAAGCTTGAACAGCATCAGGCTAATGCGGTACCAGAAGAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAg < 2:1650275/146‑1 (MQ=11) gTTGCTGATCCACGTAAAACTGCCTGTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcg < 1:436907/150‑1 (MQ=255) gTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcg < 2:1752443/150‑1 (MQ=255) tGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGGCAAAgagcg > 1:1650275/1‑150 (MQ=255) tGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcgcg < 1:683630/150‑1 (MQ=255) taTCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAg < 1:127404/149‑1 (MQ=255) ccACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCAGGTAGGAAAAGG‑CGCTCCGAGCGAAACAGCCGAAGAAGCAGGTTTTGCCCGTGCAACAGCGCGCaatata > 2:1492457/1‑146 (MQ=12) ccACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTg < 1:705294/150‑1 (MQ=255) cACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTgg < 2:1794308/150‑1 (MQ=255) cACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTgg < 2:1354900/150‑1 (MQ=255) ccGTAAAACTGCCTTTGAAGCAGCTAGCTCCCGCGCCAAAGCTCGCAAGCTGGAACATCATCAGGCTAATTCGGAACCAGAACAACATGG‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGa < 1:1311565/149‑1 (MQ=17) gTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAAc < 1:1765851/150‑1 (MQ=255) aCTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGT‑AGATCCGCGCAAAGCCGCAGTAGCAGCGGCTATTGCCCGCGCAAACGCCCGCAACCACGCACAGCac > 1:839106/1‑149 (MQ=14) tGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAA‑AGGTAGGATCAGCGCACGGCTGACGTCGAGTCCGGAGTTGAGCGAGGCAAAGCGCGGAAGCTGGAAAAGAAACa > 1:813606/1‑150 (MQ=9) ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCACGCTGGCACAGCAACAgg > 1:1405249/1‑150 (MQ=255) tGATGCAGCTCTCTCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTaa < 1:1207031/150‑1 (MQ=255) ctagaagcTATCTCCCGTCCCAAAGCTCGCAAGCTGGAACATCATCAGGCTAATCAGGACCCAGCACAACAGGT‑CGATCCCCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAAt < 1:326706/145‑1 (MQ=11) gcagcaATCGCCCGCCCCTAAGCGCGCAAGCTGGAACATCAACAGGCTAATGCGGAACCAGCACAACAGGG‑CGATCCCCGCAAAGCCGCCGTCGAAGCCGCTATGGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCg < 1:337334/150‑1 (MQ=14) cccGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCTGAAGCAGAACAACAGGG‑CGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCagaag < 2:1440803/150‑1 (MQ=14) gcCTAAGCTCGGAAGCTGGAACATCAACAGGCTACTCCGGAAGCAGAACAACTTGT‑AGATCCGCGCAAATCCGCCGTAGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAg < 2:259689/150‑1 (MQ=14) ccAAACCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAgg < 2:1566170/150‑1 (MQ=255) cacccgcgcAAGCTGGAACAGCTACAGGATAATGCGGAACCAGAAACCGAGGG‑CGATCCCCGGAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTc < 2:62887/146‑1 (MQ=14) aaatgtcgcAATCTGGAACATCAACAGGATAATTCGGAACCAGAAAAACATGC‑CGATCCTCGCAAATCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAATCAGAAGAACAGGTc < 1:1253784/144‑1 (MQ=14) gcAAGCTGGAACAGCAACAGGCTAATGAGGAACCAGAACAACAGGT‑CGATCCTCGTAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACATCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCcgc < 1:1679040/150‑1 (MQ=14) atcTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCa < 1:1681520/148‑1 (MQ=255) tGGAACAGCTCCAGGCAACTTCGGAACCAGAACAACAGGC‑CGATCCTCGCAAACCCGCCGGCGCAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAg < 2:1725055/150‑1 (MQ=14) ggAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGT‑CGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGGAGGCTTGCGCAGCAGACGTATTAGGAGGAGCCATAAGAACAGGTAGATACGCGAAAAga > 2:1679664/1‑149 (MQ=2) gCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCaa < 1:480163/78‑1 (MQ=21) gCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCaa > 2:480163/1‑78 (MQ=21) aaCAGGCTAATTCGGAACCAGAACAACAGGT‑CGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCg < 2:1048366/150‑1 (MQ=255) aGGCTAATCCGGACCCAGAACAACAGGC‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg < 2:577992/150‑1 (MQ=255) gAACAACAGGT‑CGATCCTCGCAAATCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGcc < 2:1396984/150‑1 (MQ=255) tCCTCGCAAAGCTGACGGCGAAGCCGCTATAGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCt < 2:1703678/150‑1 (MQ=11) gaaaaCCCGCCGTAGAAGCCGCTATTTCACGGGACAAATCGCGCAAGCTGGAACAGCAACAGGATACTGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAAc < 2:861786/148‑1 (MQ=11) cgTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACCACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGc < 1:1286829/150‑1 (MQ=255) gAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCAGCCGTCGATGCCGATATTGACCGTGCCAAAGCGCGAAATATGGAGCAGAAACCGGCTAAt > 2:860230/1‑150 (MQ=11) gCTATTTCCCGCGCCAAACCTGGCAATCTGGAACACCAACAGCAAAATCCGGAACCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg < 1:1714725/150‑1 (MQ=11) | CAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGT‑CGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAG > NC_000913/1708561‑1708847 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |