| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,708,847 | G→C | 17.4% | E643D (GAG→GAC) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,708,847 | 0 | G | C | 17.4% | 38.4 / 3.0 | 32 | E643D (GAG→GAC) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base G (16/9); new base C (2/3); total (19/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCA > NC_000913/1708698‑1708994 | gCTATTTCCCGCGCCAAACCTGGCAATCTGGAACACCAACAGCAAAATCCGGAACCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg < 1:1714725/150‑1 (MQ=11) cccggcccACAGCGCGCAAGCTGGCACAGCAACAGGCTAATGCGGAACCAGAACACCAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:1492152/144‑1 (MQ=11) aaatctcgcAACCTGGAACATCAACAGGCTAATTAGGAACCAGAATAACATGTCGATCCTCGCTAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc < 1:1516120/144‑1 (MQ=14) gcgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAGCAGGCTAATGCCGAGCCAGAAGAACAGGTAGATcc > 2:1417239/1‑150 (MQ=11) cAAGCTTGAACAGCAACAGGCTAATGCGTAAACAGTAGAACAGGTCGATCCGCGCAATCCCGCCGTAGAAGCCGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg < 2:1405249/150‑1 (MQ=14) cAAGCTGGAACAGCAACAGGATAATGCGGACCCAGAACAACATGGGGATCCACGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCTATCcgcg < 2:1429120/150‑1 (MQ=11) cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCAGTCCCAACGCGCGCAAGCTGGAACATCAACAGGCTAATGCCGAGCCAGAACCACAGGTAGATCCGCGCAAAGCAGCCGTc > 2:1368719/1‑150 (MQ=11) gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCGGGAGCAGAAACAGGCTAATGACGAACCAGAAGAACAGGTGGATCCGCGCAAAGCGGCAGTAGAAGCCGCTAtt > 2:1685544/1‑150 (MQ=11) cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCGAAAGCAGACCCACACCACAAGGTCCATACCCGCAAAGCAGCCGTCGACGCGGCTATTg > 1:1604542/1‑150 (MQ=11) cGGAACCAGAACCACAGGTGGATCCGCGTAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGAACGCAAGCTGGAACAGCAACAGGCTAATGCCGATCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTg < 2:869273/150‑1 (MQ=11) cAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCACAgcg > 1:862533/1‑150 (MQ=255) ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACCGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCACGAGCCAAAgcgcg > 2:1414647/1‑150 (MQ=255) gATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCCCCCGTCGTAGCCGCTATTGCCGTGGCCAAAgcgcgctcc > 1:1701486/1‑147 (MQ=11) ctccgTCGAAGCCGCGATCGCCCGCGCCATCTCTCGCAAGCTGGCAGACCTACAGGCAACTGCCGACCCAGAACAACAGGACGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAAcc < 1:559957/148‑1 (MQ=14) gCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCAAGAAGAACAGGTAGATCCGCGCAAATCCGCAGTAGAAGCCGCTATTGCCCGCGCTAAAGCACAAAAACACGAACAGCACCAGGATATTGCg > 2:436139/1‑150 (MQ=11) cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGACCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGACGCCGCTATTGCCCGCGCGCAGGCCCGAAAACCGGCCCAGCAGCAGGCTatagcagg > 2:750459/1‑143 (MQ=11) cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAATGCCCGAGCCAAAGCGCGCAAACCGGACCAGCACCCGGCTAATGAGGAGcacgaac > 1:957469/1‑144 (MQ=17) tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCGGCTATTGCCCGAGCCAAAGCCCCCAAACGGGAACCCAAACCGGCGAATCCGGAGCCAGAAGACCa > 1:1703111/1‑150 (MQ=17) caaacaggAACATCATCAGAAAAATTCCGACCCAGAAAACCATGCTGATCCTCGAAAATCTGCCGTCGAAGCAGATATTGCCCGAGCCAAAGCGCGCAAACGTGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcgcg < 2:463179/144‑1 (MQ=9) cAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCACACGGCTAATGAGGAGCAAGAACAACAGGTTGATCcgcg > 1:515375/1‑150 (MQ=14) tGGAAGAGCATCAGGCAAATGCGGAGCCAGCACAACAGGGCGATCCGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAg < 2:1698208/150‑1 (MQ=14) atcAACAGGATAATTCCGACGCAGCACCAGATGTTGATCCACGCAAAGCTGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCg < 1:1857993/148‑1 (MQ=14) gCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGCCAAAGCGAGAAAACGGGACCAGCAACAGGCTAATGCGGAGCAAGAAGATCAGGTTGATCAGCCCAAAGCTGCAGt > 2:643303/1‑150 (MQ=14) gCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGCCGCCGCTATTGCCCGCGCCAAGGCCAGAAAACCGGACCAGCACCAGGCTAATTCGGAGCCATAAGAGGAGGTTCGTCCGCGCAGAGCAGCCgg > 2:707660/1‑149 (MQ=9) gCAACACGCTAATGCCGAGCCAGAACTAGAGGTCTATCCGCGCATAGCCGCAGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGt < 1:860230/150‑1 (MQ=21) aactGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCTCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:1368719/147‑1 (MQ=255) gCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCGGGAACAGCACCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGAAGACGCGGCTAtt > 1:977914/1‑150 (MQ=14) gCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTAtt > 1:964738/1‑150 (MQ=17) ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTGGCAGCGGCTATTg > 2:807751/1‑150 (MQ=14) ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCACAAGCGCGCACACGGGAACAGCAACCGGCTAATGAGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTAGAAGCGGCTCTTg > 1:1232825/1‑150 (MQ=17) ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTg < 1:1417239/150‑1 (MQ=255) ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTg > 1:527847/1‑150 (MQ=255) gAGCCAGAACAACCGGGCGATCCGCGCACCGCCGCCTCCGAAGACGCGATTGCCCGAGCCACCGCTCGCAATCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCa < 1:1679664/150‑1 (MQ=21) | GCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCA > NC_000913/1708698‑1708994 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |