Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,708,847 G→C 17.4% E643D (GAG→GAC rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,8470GC17.4% 38.4 / 3.0 32E643D (GAG→GACrsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base G (16/9);  new base C (2/3);  total (19/13)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCA  >  NC_000913/1708698‑1708994
                                                                                                                                                     |                                                                                                                                                   
gCTATTTCCCGCGCCAAACCTGGCAATCTGGAACACCAACAGCAAAATCCGGAACCAGAACAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg                                                                                                                                                     <  1:1714725/150‑1 (MQ=11)
       cccggcccACAGCGCGCAAGCTGGCACAGCAACAGGCTAATGCGGAACCAGAACACCAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac                                                                                                                                              <  1:1492152/144‑1 (MQ=11)
               aaatctcgcAACCTGGAACATCAACAGGCTAATTAGGAACCAGAATAACATGTCGATCCTCGCTAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc                                                                                                                                      <  1:1516120/144‑1 (MQ=14)
                    gcgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAGCAGGCTAATGCCGAGCCAGAAGAACAGGTAGATcc                                                                                                                                 >  2:1417239/1‑150 (MQ=11)
                       cAAGCTTGAACAGCAACAGGCTAATGCGTAAACAGTAGAACAGGTCGATCCGCGCAATCCCGCCGTAGAAGCCGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg                                                                                                                              <  2:1405249/150‑1 (MQ=14)
                       cAAGCTGGAACAGCAACAGGATAATGCGGACCCAGAACAACATGGGGATCCACGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCTATCcgcg                                                                                                                              <  2:1429120/150‑1 (MQ=11)
                                    cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCAGTCCCAACGCGCGCAAGCTGGAACATCAACAGGCTAATGCCGAGCCAGAACCACAGGTAGATCCGCGCAAAGCAGCCGTc                                                                                                                 >  2:1368719/1‑150 (MQ=11)
                                                gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCGGGAGCAGAAACAGGCTAATGACGAACCAGAAGAACAGGTGGATCCGCGCAAAGCGGCAGTAGAAGCCGCTAtt                                                                                                     >  2:1685544/1‑150 (MQ=11)
                                                 cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCGAAAGCAGACCCACACCACAAGGTCCATACCCGCAAAGCAGCCGTCGACGCGGCTATTg                                                                                                    >  1:1604542/1‑150 (MQ=11)
                                                 cGGAACCAGAACCACAGGTGGATCCGCGTAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGAACGCAAGCTGGAACAGCAACAGGCTAATGCCGATCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTg                                                                                                    <  2:869273/150‑1 (MQ=11)
                                                               cAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCACAgcg                                                                                      >  1:862533/1‑150 (MQ=255)
                                                                 ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACCGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCACGAGCCAAAgcgcg                                                                                    >  2:1414647/1‑150 (MQ=255)
                                                                     gATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCCCCCGTCGTAGCCGCTATTGCCGTGGCCAAAgcgcgctcc                                                                                >  1:1701486/1‑147 (MQ=11)
                                                                                   ctccgTCGAAGCCGCGATCGCCCGCGCCATCTCTCGCAAGCTGGCAGACCTACAGGCAACTGCCGACCCAGAACAACAGGACGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAAcc                                                                  <  1:559957/148‑1 (MQ=14)
                                                                                             gCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCAAGAAGAACAGGTAGATCCGCGCAAATCCGCAGTAGAAGCCGCTATTGCCCGCGCTAAAGCACAAAAACACGAACAGCACCAGGATATTGCg                                                        >  2:436139/1‑150 (MQ=11)
                                                                                               cGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGACCCAGAACAACAGGTAGATCCGCGCAAAGCCGCAGTAGACGCCGCTATTGCCCGCGCGCAGGCCCGAAAACCGGCCCAGCAGCAGGCTatagcagg                                                      >  2:750459/1‑143 (MQ=11)
                                                                                                       cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAATGCCCGAGCCAAAGCGCGCAAACCGGACCAGCACCCGGCTAATGAGGAGcacgaac                                              >  1:957469/1‑144 (MQ=17)
                                                                                                           tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCGGCTATTGCCCGAGCCAAAGCCCCCAAACGGGAACCCAAACCGGCGAATCCGGAGCCAGAAGACCa                                          >  1:1703111/1‑150 (MQ=17)
                                                                                                                       caaacaggAACATCATCAGAAAAATTCCGACCCAGAAAACCATGCTGATCCTCGAAAATCTGCCGTCGAAGCAGATATTGCCCGAGCCAAAGCGCGCAAACGTGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcgcg                              <  2:463179/144‑1 (MQ=9)
                                                                                                                       cAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCACACGGCTAATGAGGAGCAAGAACAACAGGTTGATCcgcg                              >  1:515375/1‑150 (MQ=14)
                                                                                                                            tGGAAGAGCATCAGGCAAATGCGGAGCCAGCACAACAGGGCGATCCGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAg                         <  2:1698208/150‑1 (MQ=14)
                                                                                                                                  atcAACAGGATAATTCCGACGCAGCACCAGATGTTGATCCACGCAAAGCTGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCg                   <  1:1857993/148‑1 (MQ=14)
                                                                                                                                   gCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGCCAAAGCGAGAAAACGGGACCAGCAACAGGCTAATGCGGAGCAAGAAGATCAGGTTGATCAGCCCAAAGCTGCAGt                  >  2:643303/1‑150 (MQ=14)
                                                                                                                                   gCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGCCGCCGCTATTGCCCGCGCCAAGGCCAGAAAACCGGACCAGCACCAGGCTAATTCGGAGCCATAAGAGGAGGTTCGTCCGCGCAGAGCAGCCgg                  >  2:707660/1‑149 (MQ=9)
                                                                                                                                   gCAACACGCTAATGCCGAGCCAGAACTAGAGGTCTATCCGCGCATAGCCGCAGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGt                  <  1:860230/150‑1 (MQ=21)
                                                                                                                                            aactGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCTCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc         <  1:1368719/147‑1 (MQ=255)
                                                                                                                                                gCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCGGGAACAGCACCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGAAGACGCGGCTAtt     >  1:977914/1‑150 (MQ=14)
                                                                                                                                                gCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTAtt     >  1:964738/1‑150 (MQ=17)
                                                                                                                                                 ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTGGCAGCGGCTATTg    >  2:807751/1‑150 (MQ=14)
                                                                                                                                                 ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCACAAGCGCGCACACGGGAACAGCAACCGGCTAATGAGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTAGAAGCGGCTCTTg    >  1:1232825/1‑150 (MQ=17)
                                                                                                                                                 ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTg    <  1:1417239/150‑1 (MQ=255)
                                                                                                                                                 ccGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTg    >  1:527847/1‑150 (MQ=255)
                                                                                                                                                   gAGCCAGAACAACCGGGCGATCCGCGCACCGCCGCCTCCGAAGACGCGATTGCCCGAGCCACCGCTCGCAATCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCa  <  1:1679664/150‑1 (MQ=21)
                                                                                                                                                     |                                                                                                                                                   
GCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCA  >  NC_000913/1708698‑1708994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: